##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329432
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.583222030646688	31.0	31.0	34.0	30.0	34.0
2	31.668304839845554	31.0	31.0	34.0	30.0	34.0
3	31.075332693848807	31.0	31.0	34.0	27.0	34.0
4	35.307923941815005	37.0	35.0	37.0	33.0	37.0
5	35.267548386313415	37.0	35.0	37.0	33.0	37.0
6	35.32934566162364	37.0	35.0	37.0	33.0	37.0
7	32.1329105854926	36.0	35.0	37.0	16.0	37.0
8	33.64537142718376	37.0	35.0	37.0	19.0	37.0
9	36.69550316909104	39.0	35.0	39.0	32.0	39.0
10	36.59454758493407	38.0	35.0	39.0	32.0	39.0
11	36.536614536535616	38.0	35.0	39.0	32.0	39.0
12	36.458905024405645	38.0	35.0	39.0	32.0	39.0
13	32.66293195560844	37.0	34.0	39.0	8.0	39.0
14	35.27936569610724	39.0	34.0	40.0	18.0	41.0
15	36.651691396099956	39.0	35.0	41.0	30.0	41.0
16	37.29520508025936	39.0	35.0	41.0	32.0	41.0
17	37.40397107749095	39.0	36.0	41.0	32.0	41.0
18	37.31784708225066	39.0	36.0	40.0	32.0	41.0
19	37.27477294251924	39.0	36.0	40.0	32.0	41.0
20	37.16673850749168	39.0	35.0	40.0	32.0	41.0
21	37.14414203841764	39.0	35.0	40.0	32.0	41.0
22	37.17077272396124	39.0	35.0	40.0	32.0	41.0
23	37.08405983632434	39.0	35.0	40.0	32.0	41.0
24	36.94213069768571	38.0	35.0	40.0	32.0	41.0
25	36.78100791665655	38.0	35.0	40.0	32.0	41.0
26	36.63730906530027	38.0	35.0	40.0	31.0	41.0
27	36.618276305884066	38.0	35.0	40.0	31.0	41.0
28	36.56355181038879	38.0	35.0	40.0	31.0	41.0
29	36.508177711940554	38.0	35.0	40.0	31.0	41.0
30	36.346921367687415	38.0	35.0	40.0	31.0	41.0
31	36.19991986206561	38.0	35.0	40.0	30.0	41.0
32	35.999414143131204	38.0	35.0	40.0	30.0	41.0
33	35.747999587168216	38.0	35.0	40.0	29.0	41.0
34	35.51606401321062	38.0	35.0	40.0	27.0	41.0
35	35.35441305034119	38.0	34.0	40.0	26.0	41.0
36	35.06505136113067	38.0	34.0	40.0	25.0	41.0
37	34.98949403822336	38.0	34.0	40.0	24.0	41.0
38	34.82113152334928	38.0	34.0	40.0	23.0	41.0
39	34.656193083853424	38.0	34.0	40.0	23.0	41.0
40	34.52379550256199	38.0	34.0	40.0	22.0	41.0
41	34.530913815294205	38.0	34.0	40.0	23.0	41.0
42	34.49006471745307	38.0	34.0	40.0	22.0	41.0
43	34.4141856285971	38.0	33.0	40.0	22.0	41.0
44	34.19768571359188	38.0	33.0	40.0	21.0	41.0
45	34.15374341290464	38.0	33.0	40.0	20.0	41.0
46	33.98535357828019	37.0	33.0	40.0	20.0	41.0
47	33.8129841666869	37.0	33.0	40.0	20.0	41.0
48	33.742098521090846	37.0	33.0	40.0	19.0	41.0
49	33.67185640739212	37.0	33.0	40.0	19.0	41.0
50	33.52512202821827	37.0	33.0	40.0	18.0	41.0
51	31.718937443842734	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	9.0
10	18.0
11	9.0
12	5.0
13	5.0
14	13.0
15	14.0
16	32.0
17	71.0
18	183.0
19	331.0
20	523.0
21	884.0
22	1359.0
23	2204.0
24	3414.0
25	4787.0
26	6380.0
27	6842.0
28	6922.0
29	7593.0
30	8613.0
31	10693.0
32	13614.0
33	18940.0
34	26596.0
35	31876.0
36	31885.0
37	44779.0
38	53161.0
39	47663.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.86941159328784	22.577952354355375	24.714963937929525	17.837672114427257
2	33.80940527938998	27.795114014424826	26.158053862405595	12.237426843779597
3	30.02713761868914	27.430243570752083	30.5031083804852	12.039510430073582
4	26.176267029311056	25.420420602734406	34.71034993564681	13.692962432307729
5	23.938779474975107	31.39919619223391	30.612994487481483	14.049029845309501
6	20.431530634546736	41.53118094174215	29.114050851162	8.923237572549114
7	70.47099249617523	14.482503217659486	12.985988003594064	2.0605162825712133
8	76.75696350081353	7.772772529687462	12.604118604142888	2.866145365356128
9	70.63278612885209	8.684037980524053	14.422096214089706	6.261079676534156
10	36.398103402219576	29.033305811214454	22.16420991281964	12.404380873746328
11	30.39413293183419	24.941717865902525	30.15220136477331	14.511947837489982
12	28.987165788387287	23.506823866533914	32.04333519512373	15.462675149955075
13	17.902632409723402	36.645195366570334	30.289103669346023	15.163068554360231
14	16.123206003059813	33.39839481289006	32.596711916268	17.881687267782123
15	16.690849704946697	27.00162704291022	41.2667864688312	15.040736783311882
16	18.435063988926395	25.553073168362516	37.58256635663809	18.429296486073
17	17.49101483765997	25.988671410184804	34.1335996503072	22.38671410184803
18	19.02759901891741	27.77781150586464	35.08220209329998	18.11238738191797
19	23.36506471745307	27.08480050511183	31.462335170839506	18.087799606595595
20	23.698365671823016	25.64960295296146	34.418028606814154	16.23400276840137
21	20.065142426965203	29.32198450666602	33.31613200903373	17.296741057335048
22	19.024259938318075	26.272189708346488	32.464362903421645	22.239187449913793
23	18.350372762815997	29.19540299667306	33.08816386993371	19.366060370577237
24	18.198899924718912	26.49681876684718	37.08534690011899	18.21893440831492
25	17.348952135797372	30.497947983195317	33.95025377012555	18.202846110881758
26	16.54939410864761	31.301452196507928	32.620996138808614	19.528157556035843
27	18.089013817722627	31.814759950460186	32.08127929284344	18.01494693897375
28	15.17126447946769	30.540445372641393	36.32434007625246	17.963950071638457
29	17.45458850384905	28.499963573666186	34.488756404963695	19.556691517521067
30	17.00320551737536	31.647805920493454	33.433606935573955	17.915381626557224
31	22.521187984166687	29.579093712814785	30.447861774205297	17.45185652881323
32	23.01021151557833	28.91158114572962	30.98150756453532	17.096699774156733
33	19.916098011122173	30.10970397532723	29.56998712936205	20.404210884188544
34	17.365647538794047	30.194698754219385	31.720051482551785	20.719602224434787
35	19.14871657883873	27.460295296146093	32.14077563806795	21.25021248694723
36	20.41119259816897	32.1447218242308	29.547827776293744	17.896257801306493
37	17.7505524660628	32.37299351611258	31.660858690109034	18.215595327715583
38	17.414519536657032	33.347094392773016	28.98261249666092	20.255773573909032
39	20.272468976905706	29.380266640763498	30.73957599747444	19.60768838485636
40	19.119879064571748	29.725102600840238	31.89095169868137	19.26406663590665
41	16.271643313339325	28.222819883921417	31.52243862162753	23.983098181111732
42	17.965467835547244	28.171823016586124	31.79047572791957	22.07223341994706
43	19.764928725806843	27.80543480900459	30.126095825542144	22.30354063964642
44	17.831297506010348	29.94517836761456	30.545605769931278	21.67791835644382
45	16.970725369727287	32.85291046407149	29.199652735617672	20.97671143058355
46	18.885232764273052	32.642548386313415	28.7646615993589	19.707557250054638
47	17.927220185045776	28.31843901017509	32.8765875810486	20.87775322373054
48	18.952621481823257	28.417397217028096	32.45070302824255	20.179278272906092
49	18.106316326282816	28.517569635008133	32.9439762985988	20.43213774011025
50	17.392360183588725	29.4003011243595	32.534180043225916	20.673158648825858
51	17.538976177177688	28.32359940746497	30.13064911726851	24.00677529808883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	276.0
1	508.5
2	741.0
3	4142.0
4	7543.0
5	6566.0
6	5589.0
7	4178.5
8	2768.0
9	2703.5
10	2639.0
11	2523.5
12	2408.0
13	2399.0
14	2390.0
15	2319.5
16	2249.0
17	2140.5
18	2032.0
19	2063.0
20	2094.0
21	2168.5
22	2243.0
23	2432.0
24	2621.0
25	3178.5
26	4491.5
27	5247.0
28	6355.5
29	7464.0
30	8513.0
31	9562.0
32	10501.5
33	11441.0
34	12350.0
35	13259.0
36	13402.0
37	13545.0
38	15131.5
39	16718.0
40	19944.0
41	23170.0
42	24892.0
43	26614.0
44	27080.0
45	27546.0
46	31029.5
47	34513.0
48	30517.0
49	26521.0
50	24143.5
51	21766.0
52	18658.0
53	15550.0
54	13474.5
55	11399.0
56	9612.5
57	7826.0
58	7007.5
59	6189.0
60	5296.5
61	4404.0
62	3533.0
63	2662.0
64	2137.5
65	1613.0
66	1338.5
67	1064.0
68	893.0
69	722.0
70	612.5
71	503.0
72	434.5
73	366.0
74	291.5
75	157.5
76	98.0
77	78.0
78	58.0
79	42.0
80	26.0
81	19.5
82	13.0
83	13.5
84	14.0
85	10.0
86	6.0
87	6.0
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.78777931033396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95166737493643	33.69542628575942
2	9.039297217957692	7.916215034008942
3	3.7338567469313597	4.904918856330955
4	2.032735020189001	3.5603580984169283
5	1.2573345246769998	2.7527943342910057
6	0.9006441931286854	2.36623254995116
7	0.6966330587844756	2.1352810246836262
8	0.6019472689402456	2.1086347335050974
9	0.5097145096525992	2.0087339813948795
>10	4.194438938049895	30.520099828112972
>50	0.04464306335225371	1.3629610746940966
>100	0.029533103448413995	2.387888555399631
>500	0.00412089815559265	1.1049247547277385
>1k	0.0034340817963272085	3.175530888723514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4235	1.2855460307438258	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	1816	0.5512518516719687	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1687	0.5120935428252265	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	1456	0.4419728502391996	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	1365	0.41434954709924965	RNA PCR Primer, Index 40 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	692	0.21005852497632285	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	669	0.20307681099589595	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	620	0.18820272468976906	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	609	0.18486364409043443	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	543	0.16482916049442678	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	541	0.16422205493091138	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	416	0.1262779572111999	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	415	0.1259744044294422	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT	380	0.11535005706792298	No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	352	0.1068505791787076	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC	342	0.10381505136113067	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	332	0.10077952354355375	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	331	0.10047597076179605	TruSeq Adapter, Index 19 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0913693873090653	0.0
2	0.0	0.0	0.0	0.39765414410257655	0.0
3	0.0	0.0	0.0	0.48325602855824573	0.0
4	0.0	0.0	0.0	0.7634352461205954	0.0
5	0.0	0.0	0.0	1.7053595279147138	0.0
6	0.0	0.0	0.0	1.997984409529129	0.0
7	0.0	0.0	0.0	2.345855897423444	0.0
8	0.0	0.0	0.0	2.9059107797663857	0.0
9	0.0	0.0	0.0	3.0482770344107433	0.0
10	0.0	0.0	0.0	4.124978751305277	0.0
11	0.0	0.0	0.0	4.586075426795211	0.0
12	0.0	0.0	0.0	5.573229073071226	0.0
13	0.0	0.0	0.0	5.783287598047549	0.0
14	0.0	0.0	0.0	5.8867990966269215	0.0
15	0.0	0.0	0.0	6.131766191505379	0.0
16	0.0	0.0	0.0	6.455353456859078	0.0
17	0.0	0.0	0.0	6.787743752883752	0.0
18	0.0	0.0	0.0	7.193290269312028	0.0
19	3.03552781757692E-4	0.0	0.0	7.450399475460793	0.0
20	3.03552781757692E-4	0.0	0.0	7.706901576046043	0.0
21	3.03552781757692E-4	0.0	0.0	8.045059374924111	0.0
22	3.03552781757692E-4	0.0	0.0	8.407804949124554	0.0
23	3.03552781757692E-4	0.0	0.0	8.794227640302095	0.0
24	3.03552781757692E-4	0.0	0.0	9.116600694528765	0.0
25	3.03552781757692E-4	0.0	0.0	9.395565700964084	0.0
26	3.03552781757692E-4	0.0	0.0	9.642050559751329	0.0
27	3.03552781757692E-4	0.0	0.0	9.895213579737245	0.0
28	3.03552781757692E-4	0.0	0.0	10.171750163918501	0.0
29	3.03552781757692E-4	0.0	0.0	10.49685519318099	0.0
30	6.07105563515384E-4	0.0	0.0	10.873564195342286	0.0
31	6.07105563515384E-4	0.0	0.0	11.231756477816363	0.0
32	6.07105563515384E-4	0.0	0.0	11.568396512785643	0.0
33	9.10658345273076E-4	0.0	0.0	11.924463925787416	0.0
34	9.10658345273076E-4	0.0	0.0	12.312100828091989	0.0
35	9.10658345273076E-4	0.0	0.0	12.702166152650623	0.0
36	9.10658345273076E-4	0.0	0.0	13.089499502173437	0.0
37	9.10658345273076E-4	0.0	0.0	13.48624298793074	0.0
38	9.10658345273076E-4	0.0	0.0	13.891182398795502	0.0
39	9.10658345273076E-4	0.0	0.0	14.30188931251366	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.2084092E-7	45.000004	1
GCAATAG	35	1.2084092E-7	45.000004	1
CGGTTTA	175	0.0	45.0	1
TATGGGC	75	0.0	45.0	4
TCGATTG	20	7.025952E-4	45.0	1
GCGGGAG	85	0.0	45.0	5
TCGTCCC	40	6.7902874E-9	45.0	38
GGCGACT	55	1.8189894E-12	45.0	10
CCGCATG	20	7.025952E-4	45.0	1
CGCATGG	50	2.1827873E-11	44.999996	2
TACGGGT	25	3.8847276E-5	44.999996	4
GACGGGT	30	2.1607702E-6	44.999996	4
TTAACGG	30	2.1607702E-6	44.999996	2
TTACCGG	25	3.8847276E-5	44.999996	2
TAGGGCA	80	0.0	42.1875	5
GGCCTAT	75	0.0	42.0	8
TTAATCG	65	0.0	41.53846	20
CGGGATC	65	0.0	41.53846	6
TACGGCT	245	0.0	41.32653	7
ACGGGTA	60	3.6379788E-12	41.249996	5
>>END_MODULE