FastQCFastQC Report
Sat 18 Jun 2016
SRR3549437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194537
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30871.586844661940916No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCG19881.0219135691410888No Hit
GAATCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTC15620.8029320900394269No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.6332985498902522No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGC12120.6230177292751506No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCT4620.2374869562088446No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTC4310.22155168425543728No Hit
GAACTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCT4210.2164112739478865No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.20870065848656041No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4050.20818661745580533No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3890.1999619609637241No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3850.19790579684070383No Hit
GCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC3450.17734415561050085TruSeq Adapter, Index 13 (95% over 21bp)
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.1655212119031341No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2850.14650169376519634No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2790.14341744758066588No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2160.11103286264309617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.10332224718177005No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAATGCTGTCGTA1990.1022941651202599No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT253.8788225E-545.0000046
GCGCGAC302.1561773E-645.0000049
CGACCTC302.1561773E-645.00000412
CGACCCG302.1561773E-645.00000432
CGCGACC302.1561773E-645.00000410
TCGATGG253.8788225E-545.0000042
GTGGCGA302.1561773E-645.00000428
ACGGGTA253.8788225E-545.0000045
TAGCATA600.045.00000430
GGCGATC302.1561773E-645.0000048
GGCTAGA302.1561773E-645.0000048
ATGGGTA406.7648216E-945.05
CGTGGCG351.2049713E-745.027
GATCGTG207.018825E-445.029
CGACGGT207.018825E-445.028
GGGTCTA351.2049713E-745.07
CGTGAGT207.018825E-445.032
CACGACG207.018825E-445.026
ACGGGAA207.018825E-445.05
ATCGTGA207.018825E-445.030
Produced by FastQC (version 0.11.5)