##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	194537
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41845510108617	31.0	31.0	34.0	30.0	34.0
2	31.457373147524635	31.0	31.0	34.0	30.0	34.0
3	30.713571197252964	31.0	31.0	34.0	26.0	34.0
4	35.1023250075821	37.0	35.0	37.0	32.0	37.0
5	35.11433300606054	35.0	35.0	37.0	33.0	37.0
6	35.16373748952641	36.0	35.0	37.0	32.0	37.0
7	32.10971177719406	35.0	35.0	37.0	17.0	37.0
8	33.57626055711767	37.0	35.0	37.0	19.0	37.0
9	36.596796496296335	39.0	35.0	39.0	32.0	39.0
10	36.51033479492333	38.0	35.0	39.0	32.0	39.0
11	36.44643435439017	38.0	35.0	39.0	32.0	39.0
12	36.23383212448017	38.0	35.0	39.0	32.0	39.0
13	32.414723163202886	37.0	33.0	39.0	10.0	39.0
14	34.924286896580085	38.0	33.0	40.0	18.0	41.0
15	36.28743118275701	38.0	34.0	40.0	30.0	41.0
16	36.9660989940217	38.0	35.0	40.0	32.0	41.0
17	37.05349625007068	39.0	35.0	40.0	32.0	41.0
18	36.910515737366154	39.0	35.0	40.0	32.0	41.0
19	36.83979911276518	38.0	35.0	40.0	32.0	41.0
20	36.70145525015807	38.0	35.0	40.0	32.0	41.0
21	36.64977870533626	38.0	35.0	40.0	32.0	41.0
22	36.67205724360918	38.0	35.0	40.0	32.0	41.0
23	36.62056575355845	38.0	35.0	40.0	32.0	41.0
24	36.42924996273202	38.0	35.0	40.0	31.0	41.0
25	36.26043374782175	38.0	35.0	40.0	31.0	41.0
26	36.09985761063448	38.0	35.0	40.0	31.0	41.0
27	36.05917126305022	38.0	35.0	40.0	31.0	41.0
28	35.974724602517774	38.0	35.0	40.0	30.0	41.0
29	35.97476058538992	38.0	35.0	40.0	30.0	41.0
30	35.742455162771094	37.0	34.0	40.0	30.0	41.0
31	35.57308892395791	37.0	34.0	40.0	29.0	41.0
32	35.31405850815012	37.0	34.0	40.0	28.0	41.0
33	34.97373764373872	37.0	34.0	40.0	25.0	41.0
34	34.721276672303986	37.0	34.0	40.0	24.0	41.0
35	34.40705367102402	37.0	34.0	40.0	23.0	41.0
36	34.125585364223774	37.0	33.0	40.0	21.0	41.0
37	33.926409886037106	37.0	33.0	40.0	20.0	41.0
38	33.83602091118913	37.0	33.0	40.0	19.0	41.0
39	33.750926558957936	37.0	33.0	40.0	18.0	41.0
40	33.609919963811514	37.0	33.0	40.0	18.0	41.0
41	33.55920467571722	37.0	33.0	40.0	18.0	41.0
42	33.52503636840292	37.0	33.0	40.0	18.0	41.0
43	33.454083284927805	37.0	33.0	40.0	18.0	41.0
44	33.23440271002431	36.0	32.0	40.0	16.0	41.0
45	33.239440312125716	36.0	32.0	40.0	17.0	41.0
46	33.09383304975403	36.0	32.0	40.0	15.0	41.0
47	32.894426253103525	36.0	32.0	40.0	15.0	41.0
48	32.776592627623536	36.0	31.0	40.0	15.0	41.0
49	32.73434873571608	36.0	31.0	40.0	15.0	41.0
50	32.553154412785226	35.0	31.0	40.0	13.0	41.0
51	30.64464343543902	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	12.0
11	8.0
12	4.0
13	3.0
14	7.0
15	21.0
16	21.0
17	60.0
18	148.0
19	285.0
20	437.0
21	753.0
22	1092.0
23	1674.0
24	2532.0
25	3617.0
26	4595.0
27	4900.0
28	4860.0
29	5036.0
30	5863.0
31	7294.0
32	9280.0
33	12563.0
34	17852.0
35	20487.0
36	17825.0
37	24035.0
38	27015.0
39	22252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.481872343050426	21.44733392619399	24.17997604568797	19.89081768506762
2	33.82132961853015	29.190848013488434	24.977767725419845	12.01005464256157
3	28.415674139109782	27.944298513907377	32.019615805733615	11.620411541249224
4	23.450037782015762	24.91145643245244	38.79724679623928	12.841258989292525
5	24.84874342670032	28.902985036265594	32.234484956589235	14.013786580444851
6	19.463649588510155	40.555267121421636	31.549782303623473	8.431300986444738
7	66.39353953232548	14.442496800094585	16.63025542698818	2.533708240591764
8	71.77400700123884	8.828654703218412	15.752273346458514	3.645064949084236
9	66.2696556439135	8.615327675455054	17.15149303217383	7.9635236484576195
10	37.9537054647702	25.05538792106386	24.30591609822296	12.684990515942982
11	33.51804541038466	22.849123817063077	29.603108920154007	14.029721852398257
12	31.216683715694188	20.654682656769662	33.10475642165758	15.023877205878572
13	17.590484072438663	37.49415278327516	31.852552470738217	13.062810673547961
14	14.910274138081704	34.75225792522759	33.59977793427472	16.73769000241599
15	15.06191624215445	24.36657293985206	46.96947110318346	13.602039714810035
16	15.835033952410082	23.68803877925536	42.23823745611375	18.238689812220812
17	15.2089319769504	23.514806951890897	36.06049234849926	25.215768722659444
18	17.64291625757568	26.67307504485008	38.06987873772085	17.614129959853393
19	22.75608239049641	26.163146342341047	32.63440887851669	18.446362388645863
20	24.14964762487342	25.256377964089094	34.94502331176074	15.648951099276745
21	18.949094516724323	28.94924872903355	35.34443319265744	16.757223561584684
22	18.32761891054144	24.9073441042064	32.94797390727728	23.817063077974883
23	16.930455388949145	29.99326606249711	33.56071081593733	19.515567732616418
24	18.307057269311237	25.00552594108062	38.428165336157136	18.259251453451014
25	15.65460555061505	30.212761582629525	35.59528521566592	18.53734765108951
26	15.23926039776495	30.615769750741507	33.69333340187214	20.451636449621407
27	17.48099333288783	32.04069148799457	32.89759788626328	17.580717292854313
28	13.43446233878388	30.13102905873947	38.85173514549932	17.582773456977336
29	17.004477297377875	25.848039190488187	36.538550507101476	20.608933005032462
30	16.045276733988906	32.316217480479295	34.808288397579894	16.830217387951908
31	22.26671532921758	29.313189778808145	30.404498887101166	18.015596004873107
32	23.4084004585246	28.21365601402304	32.47865444619789	15.899289081254468
33	21.37074181261148	29.166688085042946	29.921300318191395	19.54126978415417
34	17.2753769205858	27.723260870682697	32.835398921541916	22.165963287189584
35	18.21915625305212	27.030847602255616	33.47383788173972	21.27615826295255
36	23.280404241866588	28.881395312973883	29.480253113803546	18.35794733135599
37	16.741288289631278	32.367107542524046	34.19400936582758	16.697594802017097
38	19.061155461428932	31.694741874296405	28.904527159357862	20.3395755049168
39	21.12348807681829	27.38862015966114	32.39897808643086	19.088913677089707
40	20.49224569105106	26.053655602790215	33.40495638361854	20.049142322540185
41	17.93077923479852	27.131085603252853	30.671800223093808	24.26633493885482
42	18.93829965507847	26.36927679567383	32.84979207040306	21.84263147884464
43	21.843145519875396	25.803317620812493	30.419406076993067	21.934130782319045
44	17.681469334882312	29.163603838858414	31.419729922842443	21.73519690341683
45	16.45702359962372	31.706050776973015	29.308563409531345	22.52836221387191
46	20.040917666048106	32.29514179821834	28.57399877658235	19.089941759151216
47	17.861383695646587	26.99949109937955	34.48855487645024	20.650570328523624
48	19.28373522774588	26.815464410369234	33.005032461691094	20.895767900193793
49	17.79764260783296	25.897901170471428	36.01731290191583	20.287143319779783
50	17.458889568565365	28.22033854742286	33.00297629756807	21.31779558644371
51	17.61772824706868	27.472922888704975	30.203508844075934	24.705840020150408
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	370.5
2	489.0
3	2913.0
4	5337.0
5	4632.5
6	3928.0
7	3045.0
8	2162.0
9	2079.5
10	1997.0
11	1962.0
12	1927.0
13	1836.5
14	1746.0
15	1625.0
16	1504.0
17	1444.5
18	1385.0
19	1348.0
20	1311.0
21	1421.5
22	1532.0
23	1469.0
24	1406.0
25	1756.0
26	2469.5
27	2833.0
28	3261.5
29	3690.0
30	4313.5
31	4937.0
32	5237.5
33	5538.0
34	5856.5
35	6175.0
36	6315.0
37	6455.0
38	7447.0
39	8439.0
40	10390.5
41	12342.0
42	13889.5
43	15437.0
44	16433.5
45	17430.0
46	20691.0
47	23952.0
48	20408.0
49	16864.0
50	15082.0
51	13300.0
52	11368.0
53	9436.0
54	7821.5
55	6207.0
56	5373.0
57	4539.0
58	3939.5
59	3340.0
60	2905.5
61	2471.0
62	2030.5
63	1590.0
64	1249.0
65	908.0
66	741.0
67	574.0
68	476.0
69	378.0
70	306.5
71	235.0
72	192.5
73	150.0
74	113.0
75	74.0
76	72.0
77	57.5
78	43.0
79	34.5
80	26.0
81	19.0
82	12.0
83	7.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	194537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.273973588571835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75805376582981	36.4444809984733
2	8.467007331704066	7.836041472830361
3	3.4914463452566094	4.8468928789896015
4	1.8529215729837814	3.4296817571978595
5	1.1719617862697178	2.71156643723302
6	0.877582759386803	2.436554485779055
7	0.7287269495667629	2.3604764132273037
8	0.5576538546989558	2.0643887795123805
9	0.4910019995556543	2.044855220343688
>10	3.534770051099756	26.765088389355242
>50	0.033325927571650746	1.0080344613107017
>100	0.02999333481448567	3.383932105460658
>500	0.0	0.0
>1k	0.00555432126194179	4.6680066002868355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3087	1.586844661940916	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCG	1988	1.0219135691410888	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTC	1562	0.8029320900394269	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.6332985498902522	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGC	1212	0.6230177292751506	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCT	462	0.2374869562088446	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTC	431	0.22155168425543728	No Hit
GAACTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCT	421	0.2164112739478865	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.20870065848656041	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	405	0.20818661745580533	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	389	0.1999619609637241	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	385	0.19790579684070383	No Hit
GCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC	345	0.17734415561050085	TruSeq Adapter, Index 13 (95% over 21bp)
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	322	0.1655212119031341	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	285	0.14650169376519634	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	279	0.14341744758066588	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	216	0.11103286264309617	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	201	0.10332224718177005	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAATGCTGTCGTA	199	0.1022941651202599	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.140410307550748E-4	0.0	0.0	0.13365066799631947	0.0
2	5.140410307550748E-4	0.0	0.0	0.5947454725836216	0.0
3	5.140410307550748E-4	0.0	0.0	0.7314803867644716	0.0
4	5.140410307550748E-4	0.0	0.0	1.1894909451672433	0.0
5	5.140410307550748E-4	0.0	0.0	2.697687329402633	0.0
6	5.140410307550748E-4	0.0	0.0	3.069338994638552	0.0
7	5.140410307550748E-4	0.0	0.0	3.6419807028997053	0.0
8	5.140410307550748E-4	0.0	0.0	4.491690526737845	0.0
9	5.140410307550748E-4	0.0	0.0	4.710157964808751	0.0
10	5.140410307550748E-4	0.0	0.0	6.427569048561456	0.0
11	5.140410307550748E-4	0.0	0.0	7.0726905421590756	0.0
12	5.140410307550748E-4	0.0	0.0	8.452376668705696	0.0
13	5.140410307550748E-4	0.0	0.0	8.760287246127985	0.0
14	5.140410307550748E-4	0.0	0.0	8.92478037596961	0.0
15	5.140410307550748E-4	0.0	0.0	9.274328276883061	0.0
16	5.140410307550748E-4	0.0	0.0	9.684018978394855	0.0
17	5.140410307550748E-4	0.0	0.0	10.094223720937405	0.0
18	5.140410307550748E-4	0.0	0.0	10.613919203030786	0.0
19	5.140410307550748E-4	0.0	0.0	10.91617532911477	0.0
20	5.140410307550748E-4	0.0	0.0	11.210720839737428	0.0
21	0.0010280820615101497	0.0	0.0	11.59470948971147	0.0
22	0.0010280820615101497	0.0	0.0	12.017765258022896	0.0
23	0.0010280820615101497	0.0	0.0	12.468065200964341	0.0
24	0.0010280820615101497	0.0	0.0	12.798593583739853	0.0
25	0.0010280820615101497	0.0	0.0	13.101877791885348	0.0
26	0.0010280820615101497	0.0	0.0	13.384086317769885	0.0
27	0.0010280820615101497	0.0	0.0	13.650359571701014	0.0
28	0.0010280820615101497	0.0	0.0	13.93256809758555	0.0
29	0.0010280820615101497	0.0	0.0	14.260526275207287	0.0
30	0.0010280820615101497	0.0	0.0	14.62755157116641	0.0
31	0.0010280820615101497	0.0	0.0	15.016166590417248	0.0
32	0.0010280820615101497	0.0	0.0	15.36879873751523	0.0
33	0.0010280820615101497	0.0	0.0	15.753301428520025	0.0
34	0.0010280820615101497	0.0	0.0	16.09205446778762	0.0
35	0.0010280820615101497	0.0	0.0	16.515110236099044	0.0
36	0.0010280820615101497	0.0	0.0	16.945362578841042	0.0
37	0.0010280820615101497	0.0	0.0	17.373044716429266	0.0
38	0.0010280820615101497	0.0	0.0	17.783249458971817	0.0
39	0.0010280820615101497	0.0	0.0	18.177518929560957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGT	25	3.8788225E-5	45.000004	6
GCGCGAC	30	2.1561773E-6	45.000004	9
CGACCTC	30	2.1561773E-6	45.000004	12
CGACCCG	30	2.1561773E-6	45.000004	32
CGCGACC	30	2.1561773E-6	45.000004	10
TCGATGG	25	3.8788225E-5	45.000004	2
GTGGCGA	30	2.1561773E-6	45.000004	28
ACGGGTA	25	3.8788225E-5	45.000004	5
TAGCATA	60	0.0	45.000004	30
GGCGATC	30	2.1561773E-6	45.000004	8
GGCTAGA	30	2.1561773E-6	45.000004	8
ATGGGTA	40	6.7648216E-9	45.0	5
CGTGGCG	35	1.2049713E-7	45.0	27
GATCGTG	20	7.018825E-4	45.0	29
CGACGGT	20	7.018825E-4	45.0	28
GGGTCTA	35	1.2049713E-7	45.0	7
CGTGAGT	20	7.018825E-4	45.0	32
CACGACG	20	7.018825E-4	45.0	26
ACGGGAA	20	7.018825E-4	45.0	5
ATCGTGA	20	7.018825E-4	45.0	30
>>END_MODULE