FastQCFastQC Report
Sat 18 Jun 2016
SRR3549436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289863
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.47505200732759956No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.1966446217695946No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG5560.19181475386648175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4020.1386862069322404No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC3870.13351134846461948No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3380.11660681080372451No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC3190.11005199007807136No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT253.883565E-545.028
CTAGACG207.0245506E-445.01
CGAACGG351.207718E-745.02
CGACGGT253.883565E-545.028
GGGTCCG253.883565E-545.07
CGGTTTA551.8189894E-1245.01
GACCGTG207.0245506E-445.012
CGGCAGG207.0245506E-445.02
TTGTCGC253.883565E-545.038
CACGACG253.883565E-545.026
ACGGGTG207.0245506E-445.05
ACGGGTA207.0245506E-445.05
TCGGCAG207.0245506E-445.01
ATCGTGA253.883565E-545.030
GTTGTCG253.883565E-545.037
CTAGTAG207.0245506E-445.01
GCGGGTA351.207718E-745.05
ACGTGCA253.883565E-545.027
CGGGCAT253.883565E-545.06
GTCCCGA207.0245506E-445.038