##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	289863
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.662164539799836	31.0	31.0	34.0	30.0	34.0
2	31.807736758399656	31.0	31.0	34.0	30.0	34.0
3	31.48074780154763	31.0	31.0	34.0	30.0	34.0
4	35.4993186436351	37.0	35.0	37.0	33.0	37.0
5	35.347012209216075	37.0	35.0	37.0	33.0	37.0
6	35.371775631936465	37.0	35.0	37.0	33.0	37.0
7	32.11169759507077	37.0	35.0	37.0	10.0	37.0
8	33.68944984354678	37.0	35.0	37.0	19.0	37.0
9	36.64971383032674	39.0	35.0	39.0	32.0	39.0
10	36.50764671586232	38.0	35.0	39.0	32.0	39.0
11	36.59000976323298	38.0	35.0	39.0	32.0	39.0
12	36.59390815661192	38.0	35.0	39.0	32.0	39.0
13	32.86955216774821	38.0	35.0	39.0	10.0	39.0
14	35.43141760072862	39.0	35.0	40.0	18.0	41.0
15	36.81722399892363	39.0	35.0	40.0	31.0	41.0
16	37.407499404891276	39.0	36.0	40.0	32.0	41.0
17	37.55402034754349	39.0	36.0	40.0	33.0	41.0
18	37.49899435250446	39.0	36.0	40.0	33.0	41.0
19	37.51357365376057	39.0	36.0	40.0	33.0	41.0
20	37.45369019157326	39.0	35.0	40.0	33.0	41.0
21	37.409821191390414	39.0	35.0	40.0	33.0	41.0
22	37.397028941258455	39.0	35.0	40.0	33.0	41.0
23	37.31581126256197	39.0	35.0	40.0	32.0	41.0
24	37.211075577082966	39.0	35.0	40.0	32.0	41.0
25	37.04689456743358	39.0	35.0	40.0	32.0	41.0
26	36.93788789876597	39.0	35.0	40.0	32.0	41.0
27	36.934776083874105	39.0	35.0	40.0	32.0	41.0
28	36.82587636228149	39.0	35.0	40.0	31.0	41.0
29	36.77545254137299	39.0	35.0	40.0	31.0	41.0
30	36.60470636128102	38.0	35.0	40.0	31.0	41.0
31	36.50314803890114	38.0	35.0	40.0	31.0	41.0
32	36.33663489303567	38.0	35.0	40.0	30.0	41.0
33	36.143095186346656	38.0	35.0	40.0	30.0	41.0
34	35.98379234327941	38.0	35.0	40.0	30.0	41.0
35	35.767717852916725	38.0	35.0	40.0	29.0	41.0
36	35.53887181185595	38.0	35.0	40.0	27.0	41.0
37	35.454421571570016	38.0	35.0	40.0	26.0	41.0
38	35.1957648958301	38.0	34.0	40.0	25.0	41.0
39	35.06032160020423	38.0	34.0	40.0	24.0	41.0
40	34.890551743409816	38.0	34.0	40.0	23.0	41.0
41	34.77388628421013	38.0	34.0	40.0	23.0	41.0
42	34.75711974277503	38.0	34.0	40.0	23.0	41.0
43	34.68795603440246	38.0	34.0	40.0	23.0	41.0
44	34.4646367421851	38.0	33.0	40.0	22.0	41.0
45	34.40934855431704	38.0	33.0	40.0	22.0	41.0
46	34.22654495399551	38.0	33.0	40.0	21.0	41.0
47	34.08076229115134	38.0	33.0	40.0	20.0	41.0
48	33.92981166965083	38.0	33.0	40.0	20.0	41.0
49	33.832431183007145	37.0	33.0	40.0	19.0	41.0
50	33.6208829688508	37.0	33.0	40.0	18.0	41.0
51	31.580339677709816	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	13.0
11	5.0
12	2.0
13	3.0
14	1.0
15	6.0
16	16.0
17	41.0
18	119.0
19	247.0
20	452.0
21	747.0
22	1184.0
23	1905.0
24	2721.0
25	3743.0
26	4584.0
27	5211.0
28	5470.0
29	6053.0
30	7355.0
31	8912.0
32	11577.0
33	15592.0
34	22167.0
35	27101.0
36	29577.0
37	42069.0
38	49801.0
39	43172.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.376840093423446	22.99293114333323	26.365213911399522	14.265014851843802
2	31.825724566433106	27.096593908156613	28.757378485698414	12.320303039711863
3	30.454387072513565	26.794727164212055	29.956565687928432	12.794320075345938
4	26.167879308500915	27.88972721596064	31.353087493057064	14.58930598248138
5	23.51283192404688	32.22246371561738	29.457019350520763	14.80768500981498
6	21.211399868213604	42.21546040715786	27.079689370495718	9.493450354132813
7	75.24175213807902	14.27605454990806	8.2773586142419	2.204834697771016
8	81.78932806187751	5.873464360749733	8.63131893342717	3.7058886439455883
9	74.4531037076136	8.123147831906797	12.002221739235432	5.421526721244174
10	30.595488213397363	37.92274281298407	18.14719367425301	13.33457529936556
11	28.116731007406948	27.698947433787684	29.02957604109528	15.154745517710092
12	27.14834249283282	24.17797373241842	31.825379575868602	16.84830419888016
13	19.526465951156236	36.572794734064026	29.7067925192246	14.193946795555142
14	16.89798284016932	34.54873509209523	31.091239654595448	17.46204241314
15	16.432935559212456	27.43709959532607	40.31076750050886	15.819197344952615
16	18.069915787803204	27.015521125497216	36.231944056330065	18.68261903036952
17	17.967108599579802	25.399930311905973	32.4246281864191	24.20833290209513
18	19.939419656872385	26.86752017332326	34.787813553299316	18.40524661650504
19	23.511451961788847	27.649958773627542	30.408503327433994	18.430085937149617
20	24.192808326692266	26.474920910913085	30.79696270307007	18.535308059324578
21	21.460483055788423	28.557973939412758	31.456584662409483	18.524958342389336
22	19.08280808519887	27.184566502106165	30.218068535825545	23.514556876869417
23	17.81462277006724	29.490483435278048	30.889075183793725	21.805818610860996
24	17.609353384184942	27.147652511703807	35.85452437875824	19.388469725353012
25	17.763564166520045	30.08007231002232	31.668753859581937	20.487609663875695
26	17.217789093468294	30.84008652363358	31.644259529501866	20.29786485339626
27	18.94584683108917	30.803517523795726	30.3574447238868	19.893190921228303
28	16.767576406785274	29.619509906404062	34.10990709404098	19.503006592769687
29	18.686413926579107	26.998271597271817	33.263645239302704	21.051669236846372
30	19.64307276195996	29.191031625285046	32.28042213045473	18.88547348230026
31	22.120450005692344	28.689760335054835	29.50186812390681	19.68792153534601
32	22.664845116486067	27.312558001538655	32.06031815029859	17.96227873167669
33	22.435426391088203	27.108668577914397	30.365724497434993	20.090180533562407
34	19.781068987763184	26.658110900666866	32.526055412384466	21.034764699185477
35	21.668857356751293	25.940185535925593	31.618385237163764	20.77257187015935
36	22.253271373027946	27.953205479830128	31.52454780361757	18.268975343524353
37	21.671962271831866	29.805114830109396	30.691050599766097	17.83187229829264
38	19.786243846230807	29.426660180844056	30.597213166219905	20.189882806705235
39	21.872746780375557	27.707227207335876	31.30202888950987	19.117997122778693
40	21.207259981439506	26.201343393258192	34.10542221670237	18.485974408599926
41	18.60706609674225	27.106598634527344	32.224188668439915	22.062146600290482
42	20.190227797269745	27.22700034154066	32.01753932029959	20.56523254089001
43	20.695638974274054	26.084046601325454	32.15139565932872	21.068918765071775
44	18.829240020285447	28.33476504417604	31.881958028447922	20.954036907090593
45	19.021054774151928	29.83685396204414	29.94311105591262	21.198980207891314
46	20.55246789000321	29.1931015686721	30.478536412029133	19.775894129295562
47	20.39756712653909	26.438006920510727	32.67957621359056	20.48484973935963
48	19.629273139379638	26.386948316963533	33.10839948527408	20.875379058382755
49	20.18367297654409	25.890506894636435	33.55792219082808	20.367897937991398
50	18.797155897786197	28.07533213966598	31.905417386834472	21.222094575713353
51	19.142491452858764	26.61981694800647	31.058465550967178	23.179226048167585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	293.0
1	396.5
2	500.0
3	1640.0
4	2780.0
5	2537.5
6	2295.0
7	1910.5
8	1526.0
9	1494.5
10	1463.0
11	1486.5
12	1510.0
13	1493.0
14	1476.0
15	1454.5
16	1433.0
17	1347.0
18	1261.0
19	1359.0
20	1457.0
21	1611.0
22	1765.0
23	1873.0
24	1981.0
25	2692.5
26	4115.0
27	4826.0
28	5673.0
29	6520.0
30	6851.0
31	7182.0
32	8232.0
33	9282.0
34	10499.5
35	11717.0
36	13098.0
37	14479.0
38	15478.5
39	16478.0
40	18314.5
41	20151.0
42	22232.0
43	24313.0
44	25492.0
45	26671.0
46	28463.5
47	30256.0
48	28902.5
49	27549.0
50	24672.0
51	21795.0
52	18701.0
53	15607.0
54	13095.0
55	10583.0
56	8695.5
57	6808.0
58	5713.0
59	4618.0
60	3906.0
61	3194.0
62	2535.5
63	1877.0
64	1484.5
65	1092.0
66	836.5
67	581.0
68	492.5
69	404.0
70	321.5
71	239.0
72	213.5
73	188.0
74	169.5
75	110.0
76	69.0
77	53.0
78	37.0
79	32.0
80	27.0
81	20.0
82	13.0
83	10.0
84	7.0
85	4.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	289863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.55571232860011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83827276228456	37.79492780400599
2	8.747837848699096	8.495149961573468
3	3.7828165488208567	5.510320564092401
4	2.0541527972413567	3.9896340920736217
5	1.2851597114140054	3.1200922621862595
6	0.8863915422703841	2.582362364219097
7	0.6663218284048598	2.264761171280525
8	0.5756784645325881	2.2361982334091652
9	0.48599167914990615	2.123790495020655
>10	3.6385861744098276	28.69038942097535
>50	0.020453248007327673	0.6814871836532203
>100	0.016221541523052982	1.6537194019404022
>500	0.0014105688280915637	0.38560531095152056
>1k	7.052844140457819E-4	0.47156173461833384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1377	0.47505200732759956	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	570	0.1966446217695946	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	556	0.19181475386648175	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	402	0.1386862069322404	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	387	0.13351134846461948	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	338	0.11660681080372451	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	319	0.11005199007807136	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4499056450806073E-4	0.0	0.0	0.13282136733560337	0.0
2	3.4499056450806073E-4	0.0	0.0	0.5478450164388003	0.0
3	3.4499056450806073E-4	0.0	0.0	0.7231002232088952	0.0
4	3.4499056450806073E-4	0.0	0.0	1.029451844492053	0.0
5	3.4499056450806073E-4	0.0	0.0	1.8615690860854957	0.0
6	3.4499056450806073E-4	0.0	0.0	2.3897496403473366	0.0
7	3.4499056450806073E-4	0.0	0.0	2.8368574119497834	0.0
8	3.4499056450806073E-4	0.0	0.0	4.020520038776939	0.0
9	3.4499056450806073E-4	0.0	0.0	4.473147659411515	0.0
10	3.4499056450806073E-4	0.0	0.0	5.712353767124469	0.0
11	3.4499056450806073E-4	0.0	0.0	6.5351562634761935	0.0
12	3.4499056450806073E-4	0.0	0.0	7.480775400792789	0.0
13	3.4499056450806073E-4	0.0	0.0	7.839565587881172	0.0
14	3.4499056450806073E-4	0.0	0.0	7.9972262758613555	0.0
15	3.4499056450806073E-4	0.0	0.0	8.257349161500432	0.0
16	3.4499056450806073E-4	0.0	0.0	8.822788696729145	0.0
17	3.4499056450806073E-4	0.0	0.0	9.401337873409162	0.0
18	3.4499056450806073E-4	0.0	0.0	10.242769860244323	0.0
19	3.4499056450806073E-4	0.0	0.0	10.585345490800826	0.0
20	3.4499056450806073E-4	0.0	0.0	10.97242490417887	0.0
21	3.4499056450806073E-4	0.0	0.0	11.408147987152551	0.0
22	3.4499056450806073E-4	0.0	0.0	11.898724569883013	0.0
23	3.4499056450806073E-4	0.0	0.0	12.391371096000524	0.0
24	3.4499056450806073E-4	0.0	0.0	12.76396090566923	0.0
25	3.4499056450806073E-4	0.0	0.0	13.092736913645412	0.0
26	3.4499056450806073E-4	0.0	0.0	13.41495810089594	0.0
27	3.4499056450806073E-4	0.0	0.0	13.743389118307615	0.0
28	3.4499056450806073E-4	0.0	0.0	14.056640550880934	0.0
29	3.4499056450806073E-4	0.0	0.0	14.399216181437438	0.0
30	3.4499056450806073E-4	0.0	0.0	14.83114436820153	0.0
31	3.4499056450806073E-4	0.0	0.0	15.21339391367646	0.0
32	3.4499056450806073E-4	0.0	0.0	15.605993176086635	0.0
33	3.4499056450806073E-4	0.0	0.0	15.994797542287218	0.0
34	3.4499056450806073E-4	0.0	0.0	16.370837257601004	0.0
35	3.4499056450806073E-4	0.0	0.0	16.7858609067042	0.0
36	3.4499056450806073E-4	0.0	0.0	17.192604782259206	0.0
37	3.4499056450806073E-4	0.0	0.0	17.594863780475603	0.0
38	3.4499056450806073E-4	0.0	0.0	17.961243759983166	0.0
39	3.4499056450806073E-4	0.0	0.0	18.363157767635055	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	25	3.883565E-5	45.0	28
CTAGACG	20	7.0245506E-4	45.0	1
CGAACGG	35	1.207718E-7	45.0	2
CGACGGT	25	3.883565E-5	45.0	28
GGGTCCG	25	3.883565E-5	45.0	7
CGGTTTA	55	1.8189894E-12	45.0	1
GACCGTG	20	7.0245506E-4	45.0	12
CGGCAGG	20	7.0245506E-4	45.0	2
TTGTCGC	25	3.883565E-5	45.0	38
CACGACG	25	3.883565E-5	45.0	26
ACGGGTG	20	7.0245506E-4	45.0	5
ACGGGTA	20	7.0245506E-4	45.0	5
TCGGCAG	20	7.0245506E-4	45.0	1
ATCGTGA	25	3.883565E-5	45.0	30
GTTGTCG	25	3.883565E-5	45.0	37
CTAGTAG	20	7.0245506E-4	45.0	1
GCGGGTA	35	1.207718E-7	45.0	5
ACGTGCA	25	3.883565E-5	45.0	27
CGGGCAT	25	3.883565E-5	45.0	6
GTCCCGA	20	7.0245506E-4	45.0	38
>>END_MODULE