FastQCFastQC Report
Sat 18 Jun 2016
SRR3549433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275176
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52331.9016920080239554No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.6984620751809751No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG13060.47460534348925776No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC12450.4524377125912143No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC9340.3394191353897142No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.22603715440300026No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5000.1817018926069134No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.18061168125127192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4500.16353170334622205No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC4080.14826874436724133TruSeq Adapter, Index 13 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3670.13336918917347443No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT3660.1330057853882606No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC3600.13082536267697764No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT3330.12101346047620433No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3280.11919644155013519No Hit
AATGTGAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTA2960.10756752042329273No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT2790.10138965607465768Illumina PCR Primer Index 1 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC2780.10102625228944385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC302.1594642E-645.0000046
CGTGGCG650.045.00000427
CAGCGCG253.883047E-545.0000041
TGGACGG253.883047E-545.0000042
GCGCGAC600.045.0000049
GGCGTAC502.1827873E-1145.00000425
TGGCGAC502.1827873E-1145.00000429
GGCCGAT302.1594642E-645.0000048
ATAGCCA253.883047E-545.00000435
CGCGAGG253.883047E-545.0000042
CGCGACC650.045.00000410
ACGGGAT302.1594642E-645.0000045
AGACGTG650.045.00000424
TGCGCGC253.883047E-545.00000415
TGCGCAG253.883047E-545.0000041
GGGCGAA302.1594642E-645.0000047
GCGGGTC253.883047E-545.0000045
ACGTGGC650.045.00000426
CCAAGCG650.045.00000420
GGCGCGA600.045.0000048
Produced by FastQC (version 0.11.5)