##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549433_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275176
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32432334215193	31.0	31.0	33.0	30.0	34.0
2	31.325893246504055	31.0	31.0	34.0	28.0	34.0
3	30.46039988952525	31.0	31.0	34.0	26.0	34.0
4	34.951500130825366	35.0	35.0	37.0	32.0	37.0
5	34.99680931476582	35.0	35.0	37.0	32.0	37.0
6	35.072575369945056	35.0	35.0	37.0	32.0	37.0
7	32.14866485449313	35.0	35.0	37.0	19.0	37.0
8	33.608795825217314	37.0	35.0	37.0	19.0	37.0
9	36.57466494171003	39.0	35.0	39.0	32.0	39.0
10	36.39561953077303	38.0	35.0	39.0	32.0	39.0
11	36.375625054510564	38.0	35.0	39.0	32.0	39.0
12	36.2188999040614	37.0	35.0	39.0	32.0	39.0
13	32.47101491409135	37.0	33.0	39.0	10.0	39.0
14	34.92404497485246	38.0	33.0	40.0	18.0	41.0
15	36.29868157106724	38.0	34.0	40.0	30.0	41.0
16	36.98277829461872	38.0	35.0	40.0	32.0	41.0
17	37.10160043027008	39.0	35.0	40.0	32.0	41.0
18	36.94328357124168	39.0	35.0	40.0	32.0	41.0
19	36.80551719626712	38.0	35.0	40.0	32.0	41.0
20	36.57785926098206	38.0	35.0	40.0	32.0	41.0
21	36.52466784894032	38.0	35.0	40.0	31.0	41.0
22	36.49787045381865	38.0	35.0	40.0	31.0	41.0
23	36.417943425298716	38.0	35.0	40.0	31.0	41.0
24	36.23235674622787	38.0	35.0	40.0	31.0	41.0
25	36.00526208680989	37.0	34.0	40.0	31.0	41.0
26	35.82005698171352	37.0	34.0	40.0	30.0	41.0
27	35.772062243800335	37.0	34.0	40.0	30.0	41.0
28	35.62805259179579	37.0	34.0	40.0	30.0	41.0
29	35.53099834287874	37.0	34.0	40.0	30.0	41.0
30	35.33265255690903	37.0	34.0	40.0	29.0	41.0
31	35.10739672064424	37.0	34.0	40.0	27.0	41.0
32	34.74859362735122	37.0	33.0	40.0	25.0	41.0
33	34.317229700264555	37.0	33.0	40.0	23.0	41.0
34	33.97166177282902	37.0	33.0	40.0	21.0	41.0
35	33.62426229031602	37.0	33.0	40.0	18.0	41.0
36	33.1904272174899	36.0	32.0	40.0	16.0	41.0
37	33.08094819315638	36.0	32.0	40.0	15.0	41.0
38	32.7764158211472	36.0	31.0	40.0	15.0	41.0
39	32.59760298863273	36.0	31.0	40.0	13.0	41.0
40	32.38807890222984	36.0	31.0	40.0	12.0	41.0
41	32.360467482629296	36.0	31.0	40.0	12.0	41.0
42	32.280038956885775	36.0	31.0	40.0	11.0	41.0
43	32.13696325260924	35.0	30.0	40.0	10.0	41.0
44	31.947684391080617	35.0	30.0	40.0	10.0	41.0
45	31.91515975230398	35.0	30.0	40.0	10.0	41.0
46	31.709066197633515	35.0	29.0	40.0	10.0	41.0
47	31.466388783905572	35.0	29.0	39.0	10.0	41.0
48	31.36672529581068	35.0	28.0	39.0	10.0	41.0
49	31.270677675378668	35.0	28.0	39.0	9.0	41.0
50	31.079785301043696	35.0	28.0	39.0	8.0	40.0
51	29.108857603860802	34.0	23.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	7.0
11	8.0
12	5.0
13	2.0
14	9.0
15	17.0
16	35.0
17	100.0
18	236.0
19	523.0
20	901.0
21	1505.0
22	2214.0
23	3347.0
24	4915.0
25	6812.0
26	8120.0
27	8374.0
28	8395.0
29	8190.0
30	8844.0
31	10562.0
32	13287.0
33	17724.0
34	24590.0
35	27970.0
36	25458.0
37	32518.0
38	34550.0
39	25946.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.79355757769573	22.009186847690206	23.68011745210338	21.517138122510683
2	34.31149518853388	28.683097363142135	25.071953949472338	11.933453498851645
3	28.353490129953197	26.728711806262172	33.26198505683635	11.65581300694828
4	24.131464953338956	24.23576183969532	38.63091257958543	13.001860627380296
5	22.64114603017705	28.341497805041136	34.85151321336163	14.165842951420181
6	19.199348780416898	38.98523127016891	32.77575079222025	9.039669157193941
7	64.97805041137309	15.358534174491961	17.191542867110503	2.4718725470244496
8	70.17399773236038	9.158138791173648	17.173009274064597	3.4948542024013722
9	65.43448556560165	7.947277378841178	19.862924092217344	6.755312963339827
10	36.540250603250286	24.732534814082623	26.08766752914498	12.63954705352211
11	30.144343983486934	24.851367851847545	30.332587144227695	14.67170102043783
12	26.89987498909789	21.844928336773556	36.0096810768381	15.245515597290463
13	17.126856993342443	37.21763525888886	33.18930429979359	12.466203447975115
14	14.258147512864493	34.485202197866094	35.52926127278542	15.727389016483995
15	13.865308021048348	25.19115039102247	47.528127452975546	13.415414134953629
16	15.01657121260575	24.171075965927262	43.769805506294155	17.042547315172836
17	15.27749513038928	23.56128441433846	36.14232345844115	25.01889699683112
18	17.347806494752447	25.72862458935372	39.571764979504024	17.3518039363898
19	20.665683053754687	27.197502689188006	34.57132889496177	17.565485362095533
20	21.68757449777597	25.77950111928366	35.700424455621125	16.83249992731924
21	17.48735354827456	28.261912376079305	36.73939587754746	17.511338198098674
22	17.384510277059047	25.25074861179754	35.17784981248365	22.186891298659766
23	16.14857400354682	28.85317033462221	34.83334302410094	20.164912637730033
24	17.61854231473675	25.168982760124432	38.721400122103674	18.491074803035147
25	15.591112597028811	29.183504375381574	36.5144489345001	18.710934093089513
26	15.828778672558652	29.84199203418903	34.97470709654912	19.3545221967032
27	17.387054103555542	29.920850655580427	34.337296857284066	18.35479838357996
28	14.221443730557898	28.819010378812106	39.54669011832427	17.412855772305726
29	16.86629647934413	26.068407128528655	37.361179754048315	19.704116638078904
30	15.632177224757973	29.587245980754133	36.0780009884583	18.702575806029596
31	20.605358025409192	28.8662528708899	31.898130650928856	18.630258452772043
32	20.901168706573248	28.08420792510975	33.042125766781986	17.972497601535018
33	18.14366078437073	29.34848969386865	31.645928423990465	20.861921097770153
34	17.27839637177661	28.477047416925892	32.45740907637294	21.787147134924556
35	16.33754397185801	27.024159083641013	33.87686426141815	22.761432683082827
36	21.08759484838794	29.77912317934703	31.39736023490421	17.735921737360815
37	16.91862662441492	29.641393144750992	33.45131842893276	19.988661801901326
38	16.2110794546036	31.85379538913277	29.685728406547085	22.249396749716546
39	18.650245660958806	29.083568334447772	31.14152397011367	21.12466203447975
40	18.624443992208622	26.980550629415355	33.53817193359886	20.856833444777163
41	16.04718434747216	27.943934064017213	31.10700061051836	24.901880977992267
42	19.055077477687007	27.422086228450155	32.449414193098235	21.073422100764603
43	19.389045556298516	27.41772538302759	31.29815100154083	21.895078059133063
44	17.580748321074513	29.270357880047676	30.527371573102307	22.621522225775504
45	16.163110154955373	30.144343983486934	29.69045265575486	24.002093205802833
46	20.151830101462338	29.776942756635755	29.516018838852226	20.555208303049685
47	17.267494258220193	27.277814925720268	33.53599151088758	21.918699305171963
48	18.43801785039393	26.645492339448207	32.43124400383755	22.48524580632032
49	18.10441317556764	25.385208012326654	34.193025554554175	22.31735325755153
50	16.392054539640085	27.428990900369215	33.53381108817629	22.645143471814404
51	16.091883013053465	27.52420269209524	30.095647876268277	26.288266418583017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	550.0
1	638.5
2	727.0
3	4580.5
4	8434.0
5	7189.0
6	5944.0
7	4619.5
8	3295.0
9	3200.0
10	3105.0
11	3115.5
12	3126.0
13	3084.5
14	3043.0
15	2897.5
16	2752.0
17	2638.0
18	2524.0
19	2487.5
20	2451.0
21	2420.0
22	2389.0
23	2484.5
24	2580.0
25	2971.0
26	3519.0
27	3676.0
28	4201.0
29	4726.0
30	5431.5
31	6137.0
32	6734.5
33	7332.0
34	7625.0
35	7918.0
36	8450.0
37	8982.0
38	10113.0
39	11244.0
40	14038.5
41	16833.0
42	19219.5
43	21606.0
44	23014.5
45	24423.0
46	27877.0
47	31331.0
48	28112.0
49	24893.0
50	22362.0
51	19831.0
52	17115.5
53	14400.0
54	12036.0
55	9672.0
56	8098.0
57	6524.0
58	5373.5
59	4223.0
60	3541.0
61	2859.0
62	2310.0
63	1761.0
64	1369.5
65	978.0
66	767.0
67	556.0
68	446.5
69	337.0
70	291.5
71	246.0
72	219.0
73	192.0
74	143.0
75	72.5
76	51.0
77	41.5
78	32.0
79	22.0
80	12.0
81	12.5
82	13.0
83	10.5
84	8.0
85	6.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	275176.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.34203385283658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87182433620103	40.71252118473684
2	8.054134335978556	8.109230067942518
3	3.041132526621555	4.592903898184342
4	1.6540749007586775	3.3307797859648254
5	1.0687825099827055	2.6902342649434496
6	0.7317765231972256	2.2103471102103467
7	0.5933204004945277	2.0908268981091847
8	0.49630465539323837	1.998798861050144
9	0.40660963765685376	1.8422600529429851
>10	3.0239563176870226	24.55096303993406
>50	0.030834639620154924	1.0930928399996056
>100	0.022946708554533894	2.7125163803689025
>500	0.0014341692846583684	0.5617082097223866
>1k	0.002151253926987553	1.614730605455164
>5k	7.170846423291842E-4	1.88908680043525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5233	1.9016920080239554	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1922	0.6984620751809751	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	1306	0.47460534348925776	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	1245	0.4524377125912143	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	934	0.3394191353897142	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	622	0.22603715440300026	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	500	0.1817018926069134	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.18061168125127192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	450	0.16353170334622205	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	408	0.14826874436724133	TruSeq Adapter, Index 13 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	367	0.13336918917347443	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	366	0.1330057853882606	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	360	0.13082536267697764	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	333	0.12101346047620433	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	328	0.11919644155013519	No Hit
AATGTGAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTA	296	0.10756752042329273	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	279	0.10138965607465768	Illumina PCR Primer Index 1 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	278	0.10102625228944385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.634037852138268E-4	0.0	0.0	0.21113759920923336	0.0
2	3.634037852138268E-4	0.0	0.0	0.7129982265895282	0.0
3	3.634037852138268E-4	0.0	0.0	0.9317673052882519	0.0
4	3.634037852138268E-4	0.0	0.0	1.4121871093409308	0.0
5	3.634037852138268E-4	0.0	0.0	2.922493240689595	0.0
6	3.634037852138268E-4	0.0	0.0	3.513751199232491	0.0
7	3.634037852138268E-4	0.0	0.0	4.075573451173067	0.0
8	3.634037852138268E-4	0.0	0.0	5.121812948803675	0.0
9	3.634037852138268E-4	0.0	0.0	5.4154432072564465	0.0
10	3.634037852138268E-4	0.0	0.0	6.8556124080588425	0.0
11	3.634037852138268E-4	0.0	0.0	7.755036776463063	0.0
12	3.634037852138268E-4	0.0	0.0	9.109442683954995	0.0
13	3.634037852138268E-4	0.0	0.0	9.542619995929877	0.0
14	3.634037852138268E-4	0.0	0.0	9.72613890746286	0.0
15	3.634037852138268E-4	0.0	0.0	10.228362938628369	0.0
16	3.634037852138268E-4	0.0	0.0	10.793092420850655	0.0
17	3.634037852138268E-4	0.0	0.0	11.408698433002877	0.0
18	3.634037852138268E-4	0.0	0.0	12.198374858272524	0.0
19	3.634037852138268E-4	0.0	0.0	12.567593104049772	0.0
20	3.634037852138268E-4	0.0	0.0	13.043652062679884	0.0
21	3.634037852138268E-4	0.0	0.0	13.572041166380789	0.0
22	3.634037852138268E-4	0.0	0.0	14.131319591824868	0.0
23	3.634037852138268E-4	0.0	0.0	14.688417594557665	0.0
24	3.634037852138268E-4	0.0	0.0	15.158662092624358	0.0
25	3.634037852138268E-4	0.0	0.0	15.541326278454516	0.0
26	3.634037852138268E-4	0.0	0.0	15.913451754513474	0.0
27	3.634037852138268E-4	0.0	0.0	16.281579788935083	0.0
28	3.634037852138268E-4	0.0	0.0	16.65552228392011	0.0
29	3.634037852138268E-4	0.0	0.0	17.089789807250632	0.0
30	3.634037852138268E-4	0.0	0.0	17.58401895514144	0.0
31	3.634037852138268E-4	0.0	0.0	18.108047213419773	0.0
32	3.634037852138268E-4	0.0	0.0	18.605546995377505	0.0
33	3.634037852138268E-4	0.0	0.0	19.035817077070675	0.0
34	3.634037852138268E-4	0.0	0.0	19.506788382707793	0.0
35	3.634037852138268E-4	0.0	0.0	20.063159577870163	0.0
36	3.634037852138268E-4	0.0	0.0	20.594092508067565	0.0
37	3.634037852138268E-4	0.0	0.0	21.115213536064193	0.0
38	3.634037852138268E-4	0.0	0.0	21.621071605081838	0.0
39	3.634037852138268E-4	0.0	0.0	22.094586737215455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	30	2.1594642E-6	45.000004	6
CGTGGCG	65	0.0	45.000004	27
CAGCGCG	25	3.883047E-5	45.000004	1
TGGACGG	25	3.883047E-5	45.000004	2
GCGCGAC	60	0.0	45.000004	9
GGCGTAC	50	2.1827873E-11	45.000004	25
TGGCGAC	50	2.1827873E-11	45.000004	29
GGCCGAT	30	2.1594642E-6	45.000004	8
ATAGCCA	25	3.883047E-5	45.000004	35
CGCGAGG	25	3.883047E-5	45.000004	2
CGCGACC	65	0.0	45.000004	10
ACGGGAT	30	2.1594642E-6	45.000004	5
AGACGTG	65	0.0	45.000004	24
TGCGCGC	25	3.883047E-5	45.000004	15
TGCGCAG	25	3.883047E-5	45.000004	1
GGGCGAA	30	2.1594642E-6	45.000004	7
GCGGGTC	25	3.883047E-5	45.000004	5
ACGTGGC	65	0.0	45.000004	26
CCAAGCG	65	0.0	45.000004	20
GGCGCGA	60	0.0	45.000004	8
>>END_MODULE