FastQCFastQC Report
Sat 18 Jun 2016
SRR3549432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165348
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20471.2379950165711107No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG8500.5140673004814089No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.48503761763069403No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC7520.45479836466119944No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC6380.3858528678907516No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2850.17236374192611947No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC2830.1711541718073397No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2790.1687350315697801No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2620.1584536855601519No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT2410.14575319931296415No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT2360.1427292740160147No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT2280.13789099354089557No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.1372862084815057No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC2260.13668142342211578No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.12035222681858868No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1850.11188523598713017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCG253.876278E-545.00000436
GCAAGAT253.876278E-545.00000437
CTCGTAC253.876278E-545.00000429
GAATGAC253.876278E-545.0000041
GTCTCGT253.876278E-545.00000427
CACGCGG253.876278E-545.00000437
CGGGGTT253.876278E-545.0000046
AGGCCGG253.876278E-545.0000042
TGTCTCG253.876278E-545.00000426
GTACTGG253.876278E-545.0000042
AGATTAG253.876278E-545.0000041
CGGGACT253.876278E-545.0000046
GGCATAA253.876278E-545.0000048
ATTGCGG253.876278E-545.0000042
TTACACG253.876278E-545.00000434
AGTACGG253.876278E-545.0000042
TCCGGGG253.876278E-545.0000044
GTTGATT207.015752E-445.029
CGTGGCG207.015752E-445.027
CCGGGTG207.015752E-445.05