##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549432_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 165348 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.456225657401358 31.0 31.0 34.0 30.0 34.0 2 31.550045963664513 31.0 31.0 34.0 30.0 34.0 3 30.96764399932264 31.0 31.0 34.0 27.0 34.0 4 35.212358177903575 37.0 35.0 37.0 32.0 37.0 5 35.14181000072574 37.0 35.0 37.0 33.0 37.0 6 35.19109998306602 37.0 35.0 37.0 32.0 37.0 7 32.09299175073179 36.0 35.0 37.0 16.0 37.0 8 33.59195152042964 37.0 35.0 37.0 19.0 37.0 9 36.60482134649346 39.0 35.0 39.0 32.0 39.0 10 36.43768294748047 38.0 35.0 39.0 32.0 39.0 11 36.38019812758546 38.0 35.0 39.0 32.0 39.0 12 36.227719718412075 38.0 35.0 39.0 32.0 39.0 13 32.519207973486225 37.0 33.0 39.0 10.0 39.0 14 34.94077944698454 38.0 33.0 40.0 18.0 41.0 15 36.338226044463795 38.0 34.0 40.0 30.0 41.0 16 37.014666037690205 38.0 35.0 40.0 32.0 41.0 17 37.1619735346058 39.0 35.0 40.0 32.0 41.0 18 37.060708324261554 39.0 36.0 40.0 32.0 41.0 19 37.03819217650047 39.0 35.0 40.0 32.0 41.0 20 36.903155768439895 38.0 35.0 40.0 32.0 41.0 21 36.83084766673924 38.0 35.0 40.0 32.0 41.0 22 36.84943271161429 38.0 35.0 40.0 32.0 41.0 23 36.79108909693495 38.0 35.0 40.0 32.0 41.0 24 36.61724363161333 38.0 35.0 40.0 31.0 41.0 25 36.465013184314294 38.0 35.0 40.0 31.0 41.0 26 36.324043834821104 38.0 35.0 40.0 31.0 41.0 27 36.28929893315915 38.0 35.0 40.0 31.0 41.0 28 36.235624259138305 38.0 35.0 40.0 31.0 41.0 29 36.21608970172001 38.0 35.0 40.0 31.0 41.0 30 36.04362919418438 38.0 35.0 40.0 30.0 41.0 31 35.858837119287806 38.0 35.0 40.0 30.0 41.0 32 35.64496093088516 38.0 34.0 40.0 29.0 41.0 33 35.33971986356049 38.0 34.0 40.0 27.0 41.0 34 35.14568667295643 38.0 34.0 40.0 26.0 41.0 35 34.906173645886255 38.0 34.0 40.0 25.0 41.0 36 34.59944480731548 38.0 34.0 40.0 23.0 41.0 37 34.49882671698478 38.0 34.0 40.0 23.0 41.0 38 34.339326753271884 38.0 33.0 40.0 22.0 41.0 39 34.23477756005516 37.0 33.0 40.0 22.0 41.0 40 34.130059027021794 37.0 33.0 40.0 21.0 41.0 41 34.091395118175 37.0 33.0 40.0 21.0 41.0 42 34.13356678036626 37.0 33.0 40.0 21.0 41.0 43 34.0654256477248 37.0 33.0 40.0 21.0 41.0 44 33.888761884026415 37.0 33.0 40.0 20.0 41.0 45 33.82536226625057 37.0 33.0 40.0 20.0 41.0 46 33.734203014248735 37.0 33.0 40.0 20.0 41.0 47 33.54459685027941 37.0 33.0 40.0 19.0 41.0 48 33.494472264557174 37.0 33.0 40.0 19.0 41.0 49 33.43579601809517 36.0 32.0 40.0 18.0 41.0 50 33.188033722814914 36.0 32.0 40.0 18.0 41.0 51 31.434453395263322 35.0 29.0 38.0 13.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 9.0 10 4.0 11 5.0 12 4.0 13 2.0 14 3.0 15 10.0 16 20.0 17 44.0 18 125.0 19 189.0 20 350.0 21 597.0 22 863.0 23 1292.0 24 1885.0 25 2748.0 26 3262.0 27 3464.0 28 3741.0 29 3946.0 30 4534.0 31 5587.0 32 7381.0 33 10266.0 34 14426.0 35 17284.0 36 16216.0 37 22208.0 38 25218.0 39 19662.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.14766432010063 22.7441517284757 26.417616179209908 17.690567772213754 2 32.491472530662605 28.06505068098798 26.99034763045214 12.453129157897283 3 29.23168106055108 27.133681689527545 31.031521397295403 12.603115852625976 4 25.543701768391514 25.045963664513632 35.58736724967947 13.82296731741539 5 23.399133947794954 31.55163654837071 30.455766020756226 14.593463483078114 6 20.855407988001065 40.16921885961729 29.481457290079106 9.493915862302538 7 69.67063405665627 14.409608825023588 13.414737402327212 2.505019715992936 8 74.89295304448798 7.990420204659264 13.42078525292111 3.695841497931635 9 69.68393932796285 8.255920845731426 15.426857294917387 6.633282531388344 10 37.95389118707212 28.533154316955756 22.332293103031184 11.18066139294095 11 31.631468176210177 23.58419817596826 30.469676077122195 14.314657570699374 12 31.108933884897304 21.467450468103635 31.75242518808815 15.671190458910903 13 18.895904395577812 36.7878655925684 29.462104168178627 14.85412584367516 14 14.604954399206521 35.145874156324844 31.750006047850594 18.499165396618043 15 14.112054575803759 28.023320511890077 43.478602704598785 14.38602220770738 16 16.451363185523864 26.320850569707527 39.147132109248375 18.080654135520234 17 15.615550233447035 26.604494762561387 33.46336212110216 24.31659288288942 18 16.486440718968478 28.398287248711807 37.34245349202893 17.77281854029078 19 21.38157098967027 28.827079855819242 31.393787647869946 18.39756150664054 20 23.58298860584948 26.5198248542468 34.06451847013571 15.832668069768005 21 18.478602704598785 30.36988654232286 33.68713259307642 17.464378160001935 22 17.966349759295547 26.055349928635362 32.58158550451169 23.396714807557395 23 18.05101966761013 29.62297699397634 32.917845997532474 19.40815734088105 24 16.967244841183444 26.736337905508385 38.359097176863344 17.93732007644483 25 15.74497423615647 30.88153470256671 34.458233543798535 18.915257517478288 26 15.943343735636356 32.52655006410722 32.417083968357645 19.113022231898782 27 16.91825725137286 32.85192442605898 32.01490190386337 18.214916418704792 28 13.796356774802234 30.618453201732105 37.636983815951815 17.94820620751385 29 16.99869366427172 26.247066792461958 36.36209691075792 20.392142632508406 30 15.879841304400417 32.03788373612018 32.97832450347147 19.103950456007933 31 20.43689672690326 29.214142293828772 31.768149599632288 18.58081137963568 32 21.410600672520985 29.506253477514093 31.223843046181386 17.859302803783535 33 18.89832353581537 31.003096499504075 29.921740813314944 20.176839151365606 34 17.069453516220335 28.67709316109055 32.3656772383095 21.887776084379613 35 17.975421535186396 26.568207658997995 32.88942109974115 22.56694970607446 36 21.022933449452065 30.005201151510754 30.940803638386917 18.031061760650267 37 16.791857173960377 32.18182258025497 33.34542903452113 17.680891211263518 38 16.090911290127487 31.31395602003048 31.44700873309626 21.148123956745774 39 18.16895275419116 31.581875801340203 32.441275370733244 17.807896073735392 40 19.93492512760965 28.594842393013526 32.31064179790503 19.159590681471805 41 15.608897597793744 29.12765803033602 31.714928514405983 23.54851585746426 42 17.916152599366185 26.964341872898373 31.86854391949101 23.25096160824443 43 19.272080702518323 27.124609913636693 31.416164695067373 22.187144688777607 44 17.403294869003556 29.026054140358514 31.72400029029683 21.8466507003411 45 16.34794494036819 32.247744151728476 29.268572949173866 22.135737958729468 46 19.008394416624334 32.35297675206232 29.027263710477296 19.611365120836055 47 17.194644023514044 28.00215303481143 33.370830007015506 21.43237293465902 48 17.65851416406609 27.848537629726394 33.6224205917217 20.870527614485813 49 18.243946101555508 26.221061034908193 34.42980864600721 21.10518421752909 50 16.435638773979726 29.16817862931514 33.80567046471684 20.59051213198829 51 16.627960422865716 28.01727266129618 29.97012361806614 25.38464329777197 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 177.0 1 271.0 2 365.0 3 1949.0 4 3533.0 5 3143.5 6 2754.0 7 2160.0 8 1566.0 9 1489.0 10 1412.0 11 1425.0 12 1438.0 13 1393.0 14 1348.0 15 1297.0 16 1246.0 17 1181.0 18 1116.0 19 1220.5 20 1325.0 21 1255.5 22 1186.0 23 1313.0 24 1440.0 25 1809.5 26 2525.5 27 2872.0 28 3052.5 29 3233.0 30 3736.0 31 4239.0 32 4846.5 33 5454.0 34 5701.0 35 5948.0 36 6317.5 37 6687.0 38 7593.5 39 8500.0 40 9896.0 41 11292.0 42 12995.5 43 14699.0 44 17181.0 45 19663.0 46 18002.5 47 16342.0 48 14966.5 49 13591.0 50 11556.5 51 9522.0 52 8215.0 53 6908.0 54 5972.0 55 5036.0 56 4243.5 57 3451.0 58 3003.0 59 2555.0 60 2233.0 61 1911.0 62 1485.5 63 1060.0 64 791.5 65 523.0 66 418.5 67 314.0 68 242.0 69 170.0 70 144.5 71 119.0 72 97.0 73 75.0 74 54.5 75 32.0 76 30.0 77 23.0 78 16.0 79 12.5 80 9.0 81 7.0 82 5.0 83 4.0 84 3.0 85 1.5 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 165348.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.95490722597189 #Duplication Level Percentage of deduplicated Percentage of total 1 78.67566558044282 36.94208578271282 2 8.672187946779324 8.144035609744297 3 3.4274011772433957 4.827999129109514 4 1.8019294426770052 3.3843771923458403 5 1.1179948221898788 2.624767157752135 6 0.7831115805200994 2.206255896654329 7 0.6118059222813277 2.010910322471394 8 0.556421386159018 2.0901371652514698 9 0.4920207627609835 2.0792510341824517 >10 3.784180630868507 29.082903935941168 >50 0.05023248625046691 1.4853521058615768 >100 0.020608199487371037 2.0441735007378377 >500 0.005152049871842759 1.8397561506640538 >1k 0.0012880124679606898 1.2379950165711107 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2047 1.2379950165711107 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG 850 0.5140673004814089 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 802 0.48503761763069403 No Hit GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC 752 0.45479836466119944 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC 638 0.3858528678907516 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 285 0.17236374192611947 No Hit GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC 283 0.1711541718073397 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 279 0.1687350315697801 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 262 0.1584536855601519 No Hit GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT 241 0.14575319931296415 No Hit CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT 236 0.1427292740160147 No Hit GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT 228 0.13789099354089557 No Hit CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 227 0.1372862084815057 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC 226 0.13668142342211578 No Hit CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.12035222681858868 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 185 0.11188523598713017 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.22558482715243003 0.0 2 0.0 0.0 0.0 0.7063889493673948 0.0 3 0.0 0.0 0.0 0.9235067856883663 0.0 4 0.0 0.0 0.0 1.376490795171396 0.0 5 0.0 0.0 0.0 2.588480054188741 0.0 6 0.0 0.0 0.0 3.0626315407504174 0.0 7 0.0 0.0 0.0 3.588794542419624 0.0 8 0.0 0.0 0.0 4.688293780390449 0.0 9 0.0 0.0 0.0 4.982219319253937 0.0 10 0.0 0.0 0.0 6.248034448556983 0.0 11 0.0 0.0 0.0 7.119529719137819 0.0 12 0.0 0.0 0.0 8.369015651837337 0.0 13 0.0 0.0 0.0 8.776035996806735 0.0 14 0.0 0.0 0.0 8.97017200087089 0.0 15 0.0 0.0 0.0 9.341510027336284 0.0 16 0.0 0.0 0.0 9.905169702687665 0.0 17 0.0 0.0 0.0 10.461571957326367 0.0 18 0.0 0.0 0.0 11.218158066623122 0.0 19 0.0 0.0 0.0 11.607034859810824 0.0 20 0.0 0.0 0.0 11.988049447226455 0.0 21 0.0 0.0 0.0 12.53175121561797 0.0 22 0.0 0.0 0.0 13.09420132085057 0.0 23 0.0 0.0 0.0 13.678423688221207 0.0 24 0.0 0.0 0.0 14.089677528606334 0.0 25 0.0 0.0 0.0 14.456177274596609 0.0 26 0.0 0.0 0.0 14.803928683745797 0.0 27 0.0 0.0 0.0 15.126279120400609 0.0 28 0.0 0.0 0.0 15.513341558410142 0.0 29 0.0 0.0 0.0 15.928828894210998 0.0 30 0.0 0.0 0.0 16.400561240535115 0.0 31 0.0 0.0 0.0 16.851730894839974 0.0 32 0.0 0.0 0.0 17.288385707719478 0.0 33 0.0 0.0 0.0 17.677867285966567 0.0 34 0.0 0.0 0.0 18.095773762004985 0.0 35 0.0 0.0 0.0 18.56206304279459 0.0 36 0.0 0.0 0.0 19.01141834192128 0.0 37 0.0 0.0 0.0 19.488593753779906 0.0 38 0.0 0.0 0.0 19.879889687205168 0.0 39 0.0 0.0 0.0 20.357065099063792 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGCG 25 3.876278E-5 45.000004 36 GCAAGAT 25 3.876278E-5 45.000004 37 CTCGTAC 25 3.876278E-5 45.000004 29 GAATGAC 25 3.876278E-5 45.000004 1 GTCTCGT 25 3.876278E-5 45.000004 27 CACGCGG 25 3.876278E-5 45.000004 37 CGGGGTT 25 3.876278E-5 45.000004 6 AGGCCGG 25 3.876278E-5 45.000004 2 TGTCTCG 25 3.876278E-5 45.000004 26 GTACTGG 25 3.876278E-5 45.000004 2 AGATTAG 25 3.876278E-5 45.000004 1 CGGGACT 25 3.876278E-5 45.000004 6 GGCATAA 25 3.876278E-5 45.000004 8 ATTGCGG 25 3.876278E-5 45.000004 2 TTACACG 25 3.876278E-5 45.000004 34 AGTACGG 25 3.876278E-5 45.000004 2 TCCGGGG 25 3.876278E-5 45.000004 4 GTTGATT 20 7.015752E-4 45.0 29 CGTGGCG 20 7.015752E-4 45.0 27 CCGGGTG 20 7.015752E-4 45.0 5 >>END_MODULE