FastQCFastQC Report
Sat 18 Jun 2016
SRR3549430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480095
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.7723471396286151No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16420.3420156427373749No Hit
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT5420.11289432299857319RNA PCR Primer, Index 16 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC5260.1095616492569179No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG5220.10872848082150406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCG302.1628512E-645.0000041
TCGCCTC207.029178E-445.024
CGAACGG207.029178E-445.02
GCGCGAG253.8874008E-545.01
GTTAGCG253.8874008E-545.01
ATCGTAC207.029178E-445.012
TACGCGG207.029178E-445.02
TGTTCCC207.029178E-445.039
GCGATGC253.8874008E-545.09
TCGTACT207.029178E-445.013
TATACGG253.8874008E-545.02
GCTCACG253.8874008E-545.044
TCCGGTA207.029178E-445.034
CGGTTTA2400.043.1250041
CGTTTTT21550.041.763341
CGGTTTT7500.041.71
CTCGTCC1300.041.5384637
CGCGAGG650.041.538462
CGGGCGA650.041.538466
TCGTCCC1300.041.5384638
Produced by FastQC (version 0.11.5)