##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549430_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480095
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46421854008061	31.0	31.0	34.0	30.0	34.0
2	31.56904570970329	31.0	31.0	34.0	30.0	34.0
3	31.103083764671574	31.0	31.0	34.0	28.0	34.0
4	35.262860475530886	37.0	35.0	37.0	33.0	37.0
5	35.15901852758308	37.0	35.0	37.0	33.0	37.0
6	35.203078557368855	37.0	35.0	37.0	32.0	37.0
7	32.0991366292088	36.0	35.0	37.0	11.0	37.0
8	33.67021110405232	37.0	35.0	37.0	19.0	37.0
9	36.68761390974703	39.0	35.0	39.0	32.0	39.0
10	36.30950957622971	38.0	35.0	39.0	32.0	39.0
11	36.30647267728262	38.0	35.0	39.0	32.0	39.0
12	36.396098688801175	38.0	35.0	39.0	32.0	39.0
13	32.80957102240182	37.0	34.0	39.0	10.0	39.0
14	35.343240400337436	38.0	34.0	40.0	18.0	41.0
15	36.66779699851071	39.0	35.0	40.0	30.0	41.0
16	37.23573251127381	39.0	36.0	40.0	32.0	41.0
17	37.34341536570887	39.0	36.0	40.0	32.0	41.0
18	37.279026026099004	39.0	36.0	40.0	32.0	41.0
19	37.278290754954746	39.0	36.0	40.0	32.0	41.0
20	37.217723575542344	39.0	35.0	40.0	32.0	41.0
21	37.15865818223477	39.0	35.0	40.0	32.0	41.0
22	37.17662545954447	39.0	35.0	40.0	32.0	41.0
23	37.072056572136766	39.0	35.0	40.0	32.0	41.0
24	36.95795623782793	39.0	35.0	40.0	32.0	41.0
25	36.79682354533998	38.0	35.0	40.0	31.0	41.0
26	36.661644049615184	38.0	35.0	40.0	31.0	41.0
27	36.65859881898374	38.0	35.0	40.0	31.0	41.0
28	36.58106624730522	38.0	35.0	40.0	31.0	41.0
29	36.548268571845156	38.0	35.0	40.0	31.0	41.0
30	36.36838542371822	38.0	35.0	40.0	30.0	41.0
31	36.26899676105771	38.0	35.0	40.0	30.0	41.0
32	36.11145919036857	38.0	35.0	40.0	30.0	41.0
33	35.8698695049938	38.0	35.0	40.0	29.0	41.0
34	35.64037325945907	38.0	35.0	40.0	27.0	41.0
35	35.44646996948521	38.0	34.0	40.0	26.0	41.0
36	35.26358949791187	38.0	34.0	40.0	25.0	41.0
37	35.16408210874931	38.0	34.0	40.0	25.0	41.0
38	35.091213197388015	38.0	34.0	40.0	25.0	41.0
39	34.939682771118214	38.0	34.0	40.0	24.0	41.0
40	34.9028067361668	38.0	34.0	40.0	24.0	41.0
41	34.825253335277395	38.0	34.0	40.0	24.0	41.0
42	34.816702944208956	38.0	34.0	40.0	24.0	41.0
43	34.79032691446485	38.0	34.0	40.0	24.0	41.0
44	34.648292525437675	38.0	34.0	40.0	23.0	41.0
45	34.610208396254905	38.0	34.0	40.0	23.0	41.0
46	34.49929493121153	38.0	33.0	40.0	23.0	41.0
47	34.310978035597124	38.0	33.0	40.0	23.0	41.0
48	34.196284068778056	38.0	33.0	40.0	22.0	41.0
49	34.12266530582489	37.0	33.0	40.0	22.0	41.0
50	33.9332361303492	37.0	33.0	40.0	21.0	41.0
51	32.09078619856487	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	18.0
10	15.0
11	8.0
12	15.0
13	5.0
14	9.0
15	15.0
16	37.0
17	93.0
18	195.0
19	369.0
20	662.0
21	1182.0
22	1783.0
23	2977.0
24	4390.0
25	6510.0
26	8205.0
27	8694.0
28	9025.0
29	9966.0
30	12064.0
31	15183.0
32	20048.0
33	27158.0
34	37967.0
35	46306.0
36	55005.0
37	72977.0
38	80400.0
39	58803.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.264655953509205	24.29539986877597	28.628917193472127	14.8110269842427
2	31.60332850789948	26.47142752996803	28.812630833480874	13.11261312865162
3	28.767431445859675	25.135858527999666	33.00138514252387	13.095324883616785
4	24.79863360376592	26.996323644278736	34.3196658994574	13.885376852497943
5	23.221654047636406	30.92013039086014	31.779960216207208	14.078255345296242
6	21.083327258146824	40.10435434653558	28.88761599266812	9.924702402649475
7	74.69521657172018	13.43192493152397	9.600183297055791	2.272675199700059
8	81.8777533613139	4.9154854768327105	10.302544288109646	2.904216873743738
9	76.52610420854205	7.001739239108927	12.275070558951874	4.19708599339714
10	34.64085233130943	29.55560878576115	21.829846176277613	13.973692706651807
11	26.501629885751775	28.573511492517106	28.005498911673733	16.919359710057385
12	25.19084764473698	24.445161895041608	32.32818504670951	18.03580541351191
13	19.27618492173424	34.79228069444589	30.012185088367925	15.919349295451942
14	18.31200074985159	30.72850165071496	32.555848321686334	18.403649277747114
15	18.023724471198406	29.001968360428666	36.55214072214874	16.422166446224185
16	20.137264499734428	27.38208062987534	34.38715254272592	18.093502327664314
17	19.506764286235015	28.821587394161575	31.235484643664275	20.436163675939138
18	20.252241743821536	28.360636957268877	33.47899894812485	17.90812235078474
19	21.801726741582396	31.106343536175128	29.348774721669667	17.743155000572802
20	23.173330278382405	28.126100042699882	32.021579062477215	16.678990616440494
21	21.11540424291026	29.85429966985701	31.482727376873328	17.547568710359407
22	20.266405607223568	26.798654432976804	31.483768837417593	21.45117112238203
23	19.02248513315073	29.837219716931024	31.34962871931597	19.79066643060228
24	19.033941199137672	28.27169622678845	34.079505097949365	18.614857476124516
25	18.395942469719532	30.865766150449392	31.15383413699372	19.584457242837356
26	17.962694883304344	31.870150699340755	30.859100802966076	19.308053614388818
27	18.242014601276832	31.54500671742051	30.826607233984944	19.386371447317718
28	16.69086326664514	30.281923369333153	33.2138430935544	19.813370270467303
29	18.092044282902343	28.915527135254482	32.126141701121654	20.866286880721525
30	18.10620814630438	31.021568647871778	31.347129214009726	19.52509399181412
31	19.234318207854695	30.959497599433444	29.914496089315655	19.891688103396202
32	19.357835428404798	30.455638988116934	29.735573167810536	20.450952415667732
33	19.059561128526646	30.91596454868307	29.081952530228396	20.942521792561887
34	17.12265280829836	30.253803934637936	30.361699247024028	22.26184401003968
35	17.734198439892104	29.905331236526106	30.727668482279547	21.632801841302243
36	19.126214603359752	32.0565721367646	28.5910080296608	20.226205230214852
37	17.534862891719346	32.88307522469511	29.622887136920816	19.959174746664722
38	17.72899113717077	30.794946833439212	29.557066830523127	21.91899519886689
39	18.915839573417763	30.46438725668878	29.18901467417907	21.43075849571439
40	19.22619481560941	29.6220539684854	30.186942167695978	20.96480904820921
41	17.75627740343057	30.195065559941263	30.17069538320541	21.87796165342276
42	18.91354836022037	29.7666086920297	29.745571189035502	21.57427175871442
43	18.91229860756725	29.70391276726481	29.6337183265812	21.75007029858674
44	17.795644612003876	30.445849259000823	28.472281527614324	23.286224601380976
45	17.70149657880211	31.1042606150866	28.225455378622982	22.96878742748831
46	18.685260208916983	31.832449827638282	28.088607463106257	21.393682500338475
47	17.313656672116977	30.833480873577106	30.17465293327362	21.678209521032297
48	17.800435330507504	29.503119174330077	30.531249023630743	22.165196471531676
49	18.443641362646975	28.52602089169852	30.478967704308523	22.551370041345983
50	17.286995282183735	29.507493308616002	30.340453451920972	22.86505795727929
51	16.916443620533435	28.82575323633864	29.115904143971505	25.141898999156414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	273.0
1	469.5
2	666.0
3	3805.5
4	6945.0
5	6330.0
6	5715.0
7	4523.0
8	3331.0
9	3260.5
10	3190.0
11	3259.5
12	3329.0
13	3316.0
14	3303.0
15	3189.0
16	3075.0
17	3045.5
18	3016.0
19	2966.0
20	2916.0
21	3036.5
22	3157.0
23	3404.0
24	3651.0
25	4250.5
26	5554.5
27	6259.0
28	7813.5
29	9368.0
30	10801.0
31	12234.0
32	13920.5
33	15607.0
34	17916.5
35	20226.0
36	21760.5
37	23295.0
38	25356.0
39	27417.0
40	29846.0
41	32275.0
42	34549.5
43	36824.0
44	39284.0
45	41744.0
46	44176.0
47	46608.0
48	45765.5
49	44923.0
50	41203.0
51	37483.0
52	32338.5
53	27194.0
54	22761.5
55	18329.0
56	15137.5
57	11946.0
58	10052.0
59	8158.0
60	6781.5
61	5405.0
62	4341.5
63	3278.0
64	2538.5
65	1799.0
66	1330.0
67	861.0
68	716.5
69	572.0
70	458.0
71	344.0
72	276.0
73	208.0
74	177.0
75	114.5
76	83.0
77	62.0
78	41.0
79	37.5
80	34.0
81	21.5
82	9.0
83	5.0
84	1.0
85	1.5
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.79040477954703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.18245952468406	43.93229413577086
2	9.016881508803921	9.880771754331594
3	3.341111078033157	5.491824851365963
4	1.6843949230494801	3.6915471856998
5	1.0072858772820579	2.7594800472502548
6	0.7229493128948337	2.3766411293162
7	0.5674353968202438	2.1763010554616837
8	0.4711833898828748	2.0653062925665466
9	0.4081626078804184	2.0127055051479292
>10	2.5659238859633944	22.34637421280116
>50	0.021845255044484584	0.8136961826413658
>100	0.008515946864454615	1.0451664737574655
>500	0.001110775677972341	0.3225571997815159
>1k	7.405171186482275E-4	1.0853339741076167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3708	0.7723471396286151	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1642	0.3420156427373749	No Hit
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT	542	0.11289432299857319	RNA PCR Primer, Index 16 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTC	526	0.1095616492569179	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCG	522	0.10872848082150406	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16371759755881649	0.0
2	0.0	0.0	0.0	0.49698497172434625	0.0
3	0.0	0.0	0.0	0.6784073985357065	0.0
4	0.0	0.0	0.0	0.9687665982774243	0.0
5	0.0	0.0	0.0	1.6675866234807695	0.0
6	0.0	0.0	0.0	2.1785271664982973	0.0
7	0.0	0.0	0.0	2.577614847061519	0.0
8	0.0	0.0	0.0	3.475978712546475	0.0
9	0.0	0.0	0.0	3.7948739312011166	0.0
10	0.0	0.0	0.0	4.594299044980681	0.0
11	0.0	0.0	0.0	5.524115018902509	0.0
12	0.0	0.0	0.0	6.3662400149970315	0.0
13	0.0	0.0	0.0	6.717628802632812	0.0
14	0.0	0.0	0.0	6.881971276518189	0.0
15	0.0	0.0	0.0	7.145044210000104	0.0
16	0.0	0.0	0.0	7.6799383455357795	0.0
17	0.0	0.0	0.0	8.343765296451744	0.0
18	0.0	0.0	0.0	9.080911069684126	0.0
19	0.0	0.0	0.0	9.568939480727773	0.0
20	0.0	0.0	0.0	10.033639175579832	0.0
21	0.0	0.0	0.0	10.671221320780262	0.0
22	0.0	0.0	0.0	11.367125256459659	0.0
23	0.0	0.0	0.0	12.151761630510627	0.0
24	0.0	0.0	0.0	12.728314187816995	0.0
25	0.0	0.0	0.0	13.23842156239911	0.0
26	0.0	0.0	0.0	13.713535862693842	0.0
27	0.0	0.0	0.0	14.197190139451568	0.0
28	0.0	0.0	0.0	14.774575865193347	0.0
29	0.0	0.0	0.0	15.326549953655006	0.0
30	2.082921088534561E-4	0.0	0.0	15.919974171778502	0.0
31	2.082921088534561E-4	0.0	0.0	16.5585977775232	0.0
32	2.082921088534561E-4	0.0	0.0	17.151188827211282	0.0
33	2.082921088534561E-4	0.0	0.0	17.726491631864526	0.0
34	2.082921088534561E-4	0.0	0.0	18.300961268082357	0.0
35	2.082921088534561E-4	0.0	0.0	18.923754673554193	0.0
36	2.082921088534561E-4	0.0	0.0	19.566335829367105	0.0
37	2.082921088534561E-4	0.0	0.0	20.17788146096085	0.0
38	2.082921088534561E-4	0.0	0.0	20.810672887657653	0.0
39	2.082921088534561E-4	0.0	0.0	21.47345837802935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGCG	30	2.1628512E-6	45.000004	1
TCGCCTC	20	7.029178E-4	45.0	24
CGAACGG	20	7.029178E-4	45.0	2
GCGCGAG	25	3.8874008E-5	45.0	1
GTTAGCG	25	3.8874008E-5	45.0	1
ATCGTAC	20	7.029178E-4	45.0	12
TACGCGG	20	7.029178E-4	45.0	2
TGTTCCC	20	7.029178E-4	45.0	39
GCGATGC	25	3.8874008E-5	45.0	9
TCGTACT	20	7.029178E-4	45.0	13
TATACGG	25	3.8874008E-5	45.0	2
GCTCACG	25	3.8874008E-5	45.0	44
TCCGGTA	20	7.029178E-4	45.0	34
CGGTTTA	240	0.0	43.125004	1
CGTTTTT	2155	0.0	41.76334	1
CGGTTTT	750	0.0	41.7	1
CTCGTCC	130	0.0	41.53846	37
CGCGAGG	65	0.0	41.53846	2
CGGGCGA	65	0.0	41.53846	6
TCGTCCC	130	0.0	41.53846	38
>>END_MODULE