SRR3549429_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571688
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5913	1.034305425336897	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGC	3828	0.6695960034144499	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG	3718	0.6503547389485174	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	2960	0.5177649347196372	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2301	0.4024922685100964	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC	1013	0.17719455367263262	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT	983	0.1719469360910147	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	872	0.15253075103902827	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	858	0.15008186283427324	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTA	731	0.12786694840542395	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	687	0.12017044261905097	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	684	0.11964568086088914	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	627	0.10967520745581505	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	594	0.10390282811603532	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATCG	20	7.0303096E-4	45.0	13
CGCGGGT	25	3.8883372E-5	45.0	4
CGACGGG	45	3.8380676E-10	45.0	3
CGATTCA	20	7.0303096E-4	45.0	10
CGCGACC	45	3.8380676E-10	45.0	10
ACGGGCG	45	3.8380676E-10	45.0	5
TGTTGCG	20	7.0303096E-4	45.0	1
AATGCGG	70	0.0	45.0	2
GCCGATG	20	7.0303096E-4	45.0	9
ACGATCC	20	7.0303096E-4	45.0	10
TGCGCGG	55	1.8189894E-12	45.0	2
GTCACGG	20	7.0303096E-4	45.0	2
GCGGGTT	35	1.210501E-7	45.0	5
GGACGTA	20	7.0303096E-4	45.0	8
GCGATAA	20	7.0303096E-4	45.0	9
CTCGATC	25	3.8883372E-5	45.0	16
GGATTCG	25	3.8883372E-5	45.0	8
CGTGGCG	30	2.1635806E-6	44.999996	27
CGTGCGG	30	2.1635806E-6	44.999996	2
TAAACGG	30	2.1635806E-6	44.999996	2