##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571688
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51534053539693	31.0	31.0	34.0	30.0	34.0
2	31.608503239529252	31.0	31.0	34.0	30.0	34.0
3	31.123691594016318	31.0	31.0	34.0	28.0	34.0
4	35.28836008452163	37.0	35.0	37.0	33.0	37.0
5	35.193777025230546	37.0	35.0	37.0	33.0	37.0
6	35.238306558822295	37.0	35.0	37.0	32.0	37.0
7	32.07530331229622	36.0	35.0	37.0	11.0	37.0
8	33.63036306516841	37.0	35.0	37.0	19.0	37.0
9	36.67710009655617	39.0	35.0	39.0	32.0	39.0
10	36.48536964218245	38.0	35.0	39.0	32.0	39.0
11	36.41236478638698	38.0	35.0	39.0	32.0	39.0
12	36.52759547165587	38.0	35.0	39.0	32.0	39.0
13	32.896891311344646	38.0	34.0	39.0	10.0	39.0
14	35.470529379661635	39.0	35.0	40.0	18.0	41.0
15	36.75194511691692	39.0	35.0	40.0	30.0	41.0
16	37.341738850561846	39.0	36.0	40.0	32.0	41.0
17	37.44360560305621	39.0	36.0	40.0	32.0	41.0
18	37.355973887854915	39.0	36.0	40.0	32.0	41.0
19	37.35676452890388	39.0	36.0	40.0	32.0	41.0
20	37.29761513272974	39.0	35.0	40.0	32.0	41.0
21	37.29595163795637	39.0	35.0	40.0	32.0	41.0
22	37.33245056744238	39.0	35.0	40.0	32.0	41.0
23	37.25439575432753	39.0	35.0	40.0	32.0	41.0
24	37.13267201690432	39.0	35.0	40.0	32.0	41.0
25	36.97388260729629	39.0	35.0	40.0	32.0	41.0
26	36.822170484599994	39.0	35.0	40.0	31.0	41.0
27	36.84224087264382	39.0	35.0	40.0	31.0	41.0
28	36.76449217055457	39.0	35.0	40.0	31.0	41.0
29	36.72400330250066	38.0	35.0	40.0	31.0	41.0
30	36.60683799555002	38.0	35.0	40.0	31.0	41.0
31	36.477758847483244	38.0	35.0	40.0	30.0	41.0
32	36.27058640377269	38.0	35.0	40.0	30.0	41.0
33	36.08316074502176	38.0	35.0	40.0	30.0	41.0
34	35.86912441751445	38.0	35.0	40.0	29.0	41.0
35	35.7303494213627	38.0	35.0	40.0	28.0	41.0
36	35.51963308657869	38.0	35.0	40.0	27.0	41.0
37	35.409454107835046	38.0	35.0	40.0	26.0	41.0
38	35.28961076671191	38.0	34.0	40.0	25.0	41.0
39	35.08426449391976	38.0	34.0	40.0	24.0	41.0
40	35.067134520927496	38.0	34.0	40.0	24.0	41.0
41	34.9826811127748	38.0	34.0	40.0	24.0	41.0
42	35.00853962301115	38.0	34.0	40.0	24.0	41.0
43	34.946694700605924	38.0	34.0	40.0	24.0	41.0
44	34.77863275073117	38.0	34.0	40.0	23.0	41.0
45	34.78053238829571	38.0	34.0	40.0	23.0	41.0
46	34.672574900994945	38.0	34.0	40.0	23.0	41.0
47	34.47464351184562	38.0	33.0	40.0	23.0	41.0
48	34.33076783140454	38.0	33.0	40.0	22.0	41.0
49	34.24842221631379	38.0	33.0	40.0	22.0	41.0
50	34.07497446159444	37.0	33.0	40.0	21.0	41.0
51	32.095823246246205	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	22.0
11	10.0
12	5.0
13	3.0
14	9.0
15	16.0
16	40.0
17	103.0
18	186.0
19	440.0
20	754.0
21	1195.0
22	1992.0
23	3082.0
24	4914.0
25	7186.0
26	9082.0
27	10216.0
28	10821.0
29	12028.0
30	14340.0
31	18045.0
32	23647.0
33	31129.0
34	42815.0
35	52658.0
36	62333.0
37	86493.0
38	99249.0
39	78865.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.47719735240201	22.00465988441248	27.63517862890248	15.882964134283037
2	30.960069128615608	29.070751878647094	27.629231328976644	12.339947663760654
3	28.709890709617834	27.618911014399462	31.438651852059163	12.232546423923539
4	24.481885224108254	25.613446495291136	36.39135332559018	13.513314955010426
5	24.57161948475392	30.453674031989475	30.4258616588069	14.5488448244497
6	21.080029666531395	41.33828241978142	28.08192580568422	9.499762108002967
7	72.3329858244357	13.239739158422076	12.067771231860736	2.359503785281482
8	78.8041729055009	6.539056268454122	11.350246987867509	3.3065238381774673
9	73.04508753026127	7.331096682106324	12.834273239949063	6.789542547683351
10	32.507941394606846	37.58221267544535	19.01316802171814	10.896677908229664
11	23.684947034046544	24.347371293432783	35.462874854815915	16.50480681770476
12	24.18049705433733	21.945886567498356	36.00180518044807	17.871811197716237
13	18.644260505730397	32.07658722939785	33.72608835588643	15.55306390898532
14	16.69389597122906	29.968444326275872	34.03324890499713	19.304410797497937
15	15.47330012174473	28.080701361581845	40.61271182882971	15.83328668784372
16	18.700584934439764	27.6894040105792	35.50275674843621	18.107254306544828
17	18.293544730692265	27.46760470746281	34.375043730146515	19.863806831698408
18	17.477540196750674	29.070751878647094	34.99006451071214	18.461643413890094
19	20.587103455031418	28.50103552986944	33.15374819831796	17.758112816781182
20	21.81224723975315	28.103615958354904	35.06860385385035	15.015532948041589
21	20.19440673934034	28.65199199563398	34.523026545948134	16.63057471907754
22	18.711080169603	25.40371671261247	34.340759295279945	21.544443822504583
23	18.257860931137262	28.706392297896755	34.974496578553335	18.061250192412643
24	18.45482151103399	26.90296105568072	35.566952603518004	19.075264829767285
25	17.33183134856775	30.913365332139207	32.84256447572802	18.91223884356502
26	16.651390268817956	30.743692363666895	33.302605617049856	19.30231175046529
27	18.143637788444046	30.411693091336534	33.037251088006045	18.407418032213375
28	15.581226123340002	28.978743650382725	36.87833223716433	18.561697989112943
29	17.652460782804607	26.681511593736445	35.559605938903736	20.10642168455521
30	17.877933418228125	28.362148584542616	34.31697009557661	19.442947901652648
31	18.968563272274388	27.762170974377632	34.01820573459649	19.251060018751488
32	19.739088453841955	29.848623724828926	32.83591749345797	17.576370327871146
33	19.51588978603714	28.358475252235486	31.885049187668795	20.240585774058577
34	17.761961069674367	27.116189250080463	35.979415345433175	19.142434334811995
35	18.095534627279214	26.76057569863282	33.78328738752606	21.3606022865619
36	19.830746840934214	27.67173703138775	34.247001861155034	18.250514266523
37	17.438707826646702	29.667755838849164	34.07085683099872	18.82267950350541
38	18.747288730916164	28.844579560879364	32.63510866066805	19.773023047536416
39	20.286764808776816	27.978372818740294	32.702802927470934	19.032059445011964
40	19.9257287191615	26.439946264395964	35.2244580960244	18.409866920418132
41	17.60225857460713	27.636403073004857	34.504659884412476	20.25667846797554
42	18.84139600621318	27.236534613285567	34.501336393277455	19.4207329872238
43	20.7978827612264	26.582156700857812	32.0452764444942	20.574684093421585
44	18.55907418032213	28.184954030869985	32.42607856033361	20.829893228474273
45	17.59298778354627	27.792782076937073	32.04929961797344	22.564930521543218
46	20.171492142567274	28.473398077272915	31.71082828395908	19.644281496200726
47	17.258364702425098	26.41545738234841	35.91731853738403	20.408859377842457
48	18.166027623458948	26.780341724856914	34.10706539231189	20.946565259372246
49	18.564846559661913	25.593855389653097	35.56083038300611	20.280467667678874
50	18.249989504764837	25.85746071283637	33.95435972068681	21.938190061711982
51	17.177726312254237	25.377478624704388	32.809504484963824	24.635290578077555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	235.0
1	571.0
2	907.0
3	5640.0
4	10373.0
5	8967.5
6	7562.0
7	5819.0
8	4076.0
9	3951.5
10	3827.0
11	3666.0
12	3505.0
13	3539.0
14	3573.0
15	3522.0
16	3471.0
17	3421.0
18	3371.0
19	3580.0
20	3789.0
21	4199.0
22	4609.0
23	5059.5
24	5510.0
25	6764.5
26	9093.5
27	10168.0
28	12271.0
29	14374.0
30	16520.5
31	18667.0
32	20773.0
33	22879.0
34	24055.5
35	25232.0
36	26649.5
37	28067.0
38	30044.0
39	32021.0
40	35672.0
41	39323.0
42	43703.5
43	48084.0
44	57256.5
45	66429.0
46	60376.0
47	54323.0
48	49503.0
49	44683.0
50	38067.0
51	31451.0
52	27355.5
53	23260.0
54	19673.5
55	16087.0
56	13872.5
57	11658.0
58	10118.5
59	8579.0
60	7424.5
61	6270.0
62	4841.5
63	3413.0
64	2532.0
65	1651.0
66	1253.0
67	855.0
68	678.5
69	502.0
70	432.5
71	363.0
72	287.0
73	211.0
74	184.0
75	112.0
76	67.0
77	51.5
78	36.0
79	29.0
80	22.0
81	16.5
82	11.0
83	10.0
84	9.0
85	6.0
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	571688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.59688212634197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29828661357713	36.018591499010505
2	9.942439914170805	9.265734014577088
3	3.736710392819463	5.223571611434565
4	1.9049096825072394	3.5505140774846935
5	1.1377776006202494	2.6508444371046975
6	0.7616005185070347	2.129292575293993
7	0.5603211447437096	1.8276452834163743
8	0.5142473006000715	1.916985667988086
9	0.43317048598199215	1.8165954668321838
>10	3.650618210816823	28.280933755778936
>50	0.0358047397695245	1.1244602951456755
>100	0.01826772415609395	1.6326380213201996
>500	0.003653544831218789	1.2027724317650894
>1k	0.0018267724156093945	2.35276928619866
>5k	3.653544831218789E-4	1.0066515766492532
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5913	1.034305425336897	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGC	3828	0.6695960034144499	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG	3718	0.6503547389485174	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	2960	0.5177649347196372	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2301	0.4024922685100964	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC	1013	0.17719455367263262	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT	983	0.1719469360910147	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	872	0.15253075103902827	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	858	0.15008186283427324	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTA	731	0.12786694840542395	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	687	0.12017044261905097	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	684	0.11964568086088914	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	627	0.10967520745581505	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	594	0.10390282811603532	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7492058605393152E-4	0.0	0.0	0.20728089447390885	0.0
2	1.7492058605393152E-4	0.0	0.0	0.6638236240746701	0.0
3	1.7492058605393152E-4	0.0	0.0	0.8679559479996082	0.0
4	1.7492058605393152E-4	0.0	0.0	1.328346930493556	0.0
5	1.7492058605393152E-4	0.0	0.0	2.623109108464757	0.0
6	1.7492058605393152E-4	0.0	0.0	3.065308350009096	0.0
7	1.7492058605393152E-4	0.0	0.0	3.5771259848028993	0.0
8	3.4984117210786305E-4	0.0	0.0	4.51679937308462	0.0
9	3.4984117210786305E-4	0.0	0.0	4.793873581394047	0.0
10	3.4984117210786305E-4	0.0	0.0	6.161577643749737	0.0
11	3.4984117210786305E-4	0.0	0.0	7.107723093715453	0.0
12	3.4984117210786305E-4	0.0	0.0	8.718042008927947	0.0
13	3.4984117210786305E-4	0.0	0.0	9.100068568869734	0.0
14	3.4984117210786305E-4	0.0	0.0	9.303151369278348	0.0
15	3.4984117210786305E-4	0.0	0.0	9.748849022543766	0.0
16	3.4984117210786305E-4	0.0	0.0	10.339206000475784	0.0
17	3.4984117210786305E-4	0.0	0.0	11.005478512755209	0.0
18	3.4984117210786305E-4	0.0	0.0	11.739095450665397	0.0
19	3.4984117210786305E-4	0.0	0.0	12.244266103189153	0.0
20	3.4984117210786305E-4	0.0	0.0	12.747162788094204	0.0
21	3.4984117210786305E-4	0.0	0.0	13.364457536278529	0.0
22	3.4984117210786305E-4	0.0	0.0	14.101747806495851	0.0
23	3.4984117210786305E-4	0.0	0.0	14.790759994962286	0.0
24	3.4984117210786305E-4	0.0	0.0	15.310623976714572	0.0
25	3.4984117210786305E-4	0.0	0.0	15.753347980017072	0.0
26	3.4984117210786305E-4	0.0	0.0	16.178230083542072	0.0
27	3.4984117210786305E-4	0.0	0.0	16.602937266481018	0.0
28	3.4984117210786305E-4	0.0	0.0	17.092714907432025	0.0
29	3.4984117210786305E-4	0.0	0.0	17.598410321713942	0.0
30	3.4984117210786305E-4	0.0	0.0	18.158331117672578	0.0
31	3.4984117210786305E-4	0.0	0.0	18.70933096374246	0.0
32	3.4984117210786305E-4	0.0	0.0	19.23339303956004	0.0
33	3.4984117210786305E-4	0.0	0.0	19.753257021312326	0.0
34	3.4984117210786305E-4	0.0	0.0	20.271721638376178	0.0
35	3.4984117210786305E-4	0.0	0.0	20.816599263934172	0.0
36	3.4984117210786305E-4	0.0	0.0	21.418326079959698	0.0
37	3.4984117210786305E-4	0.0	0.0	22.023551307706303	0.0
38	3.4984117210786305E-4	0.0	0.0	22.586795594799963	0.0
39	3.4984117210786305E-4	0.0	0.0	23.181525587383327	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATCG	20	7.0303096E-4	45.0	13
CGCGGGT	25	3.8883372E-5	45.0	4
CGACGGG	45	3.8380676E-10	45.0	3
CGATTCA	20	7.0303096E-4	45.0	10
CGCGACC	45	3.8380676E-10	45.0	10
ACGGGCG	45	3.8380676E-10	45.0	5
TGTTGCG	20	7.0303096E-4	45.0	1
AATGCGG	70	0.0	45.0	2
GCCGATG	20	7.0303096E-4	45.0	9
ACGATCC	20	7.0303096E-4	45.0	10
TGCGCGG	55	1.8189894E-12	45.0	2
GTCACGG	20	7.0303096E-4	45.0	2
GCGGGTT	35	1.210501E-7	45.0	5
GGACGTA	20	7.0303096E-4	45.0	8
GCGATAA	20	7.0303096E-4	45.0	9
CTCGATC	25	3.8883372E-5	45.0	16
GGATTCG	25	3.8883372E-5	45.0	8
CGTGGCG	30	2.1635806E-6	44.999996	27
CGTGCGG	30	2.1635806E-6	44.999996	2
TAAACGG	30	2.1635806E-6	44.999996	2
>>END_MODULE