##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	154647
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495625521348618	31.0	31.0	34.0	30.0	34.0
2	31.547595491668122	31.0	31.0	34.0	30.0	34.0
3	30.75876027339683	31.0	31.0	34.0	26.0	34.0
4	35.156084502124195	37.0	35.0	37.0	32.0	37.0
5	35.169935401268695	37.0	35.0	37.0	33.0	37.0
6	35.27455430755204	37.0	35.0	37.0	33.0	37.0
7	32.1769772449514	36.0	35.0	37.0	16.0	37.0
8	33.750509224233255	37.0	35.0	37.0	19.0	37.0
9	36.751537372208965	39.0	37.0	39.0	32.0	39.0
10	36.612970183708704	38.0	35.0	39.0	32.0	39.0
11	36.601182046855094	38.0	35.0	39.0	32.0	39.0
12	36.467167161341635	38.0	35.0	39.0	32.0	39.0
13	32.64375642592485	37.0	34.0	39.0	8.0	39.0
14	35.16910124347708	39.0	34.0	40.0	18.0	41.0
15	36.6149036192102	39.0	35.0	40.0	30.0	41.0
16	37.24614121192134	39.0	35.0	41.0	32.0	41.0
17	37.40615078210376	39.0	35.0	40.0	33.0	41.0
18	37.29457409455082	39.0	36.0	40.0	32.0	41.0
19	37.220120661894505	39.0	36.0	40.0	32.0	41.0
20	37.03606924156304	38.0	35.0	40.0	32.0	41.0
21	36.95570557463126	38.0	35.0	40.0	32.0	41.0
22	36.94757091957813	38.0	35.0	40.0	32.0	41.0
23	36.86422627015073	38.0	35.0	40.0	32.0	41.0
24	36.7032532153873	38.0	35.0	40.0	32.0	41.0
25	36.56398119588482	38.0	35.0	40.0	32.0	41.0
26	36.37160113031614	38.0	35.0	40.0	31.0	41.0
27	36.33783390560438	38.0	35.0	40.0	31.0	41.0
28	36.26255278149592	38.0	35.0	40.0	31.0	41.0
29	36.22656113600652	38.0	35.0	40.0	31.0	41.0
30	36.042962359437944	38.0	35.0	40.0	30.0	41.0
31	35.86380595808519	38.0	35.0	40.0	30.0	41.0
32	35.57898956979444	38.0	34.0	40.0	29.0	41.0
33	35.245488111634884	38.0	34.0	40.0	27.0	41.0
34	34.95717990003039	38.0	34.0	40.0	25.0	41.0
35	34.68043350339806	38.0	34.0	40.0	23.0	41.0
36	34.37098036172703	38.0	34.0	40.0	21.0	41.0
37	34.2235801535109	38.0	33.0	40.0	21.0	41.0
38	34.105045684688356	38.0	33.0	40.0	20.0	41.0
39	34.0202073108434	38.0	33.0	40.0	18.0	41.0
40	33.895549218542875	37.0	33.0	40.0	18.0	41.0
41	33.86459485150051	37.0	33.0	40.0	18.0	41.0
42	33.83309731194268	37.0	33.0	40.0	18.0	41.0
43	33.77345179667242	37.0	33.0	40.0	18.0	41.0
44	33.57559474157274	37.0	33.0	40.0	17.0	41.0
45	33.573848829915875	37.0	33.0	40.0	18.0	41.0
46	33.517054970351836	37.0	33.0	40.0	18.0	41.0
47	33.40443720214424	37.0	33.0	40.0	18.0	41.0
48	33.32578711517197	37.0	33.0	40.0	17.0	41.0
49	33.2633093432139	37.0	33.0	40.0	15.0	41.0
50	33.07047016754286	36.0	32.0	40.0	15.0	41.0
51	31.432151933112184	35.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	8.0
10	9.0
11	3.0
12	2.0
13	4.0
14	5.0
15	6.0
16	22.0
17	50.0
18	98.0
19	195.0
20	328.0
21	613.0
22	902.0
23	1320.0
24	2033.0
25	2762.0
26	3533.0
27	3618.0
28	3388.0
29	3489.0
30	3901.0
31	4833.0
32	6522.0
33	9056.0
34	13201.0
35	15803.0
36	14661.0
37	20901.0
38	23589.0
39	19788.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.964713185512814	22.601796349104735	26.084566787587214	20.348923677795234
2	34.964144147639466	26.09943936836796	26.215186844878986	12.721229639113593
3	29.01446520139414	25.187038869166557	33.01777596720273	12.780719962236578
4	24.250712913926556	25.969465945023178	37.31659844678526	12.463222694265005
5	21.874333158742168	30.372396490070937	33.69674161154112	14.056528739645774
6	19.715869043693054	38.94999579687934	32.097615860637454	9.236519298790148
7	67.84677361992149	15.04264550880392	14.631386318518949	2.4791945527556307
8	74.57435320439453	6.937735617244433	15.279960167348866	3.2079510110121765
9	68.67381843812036	9.217766914327468	17.55352512496201	4.554889522590157
10	35.140028581220456	29.26406590493188	24.586315932413818	11.009589581433845
11	30.27216822828765	24.6167077279223	31.339760874766405	13.771363169023648
12	28.769390935485333	22.926406590493187	34.00259946846689	14.301603005554586
13	18.105750515690573	36.882060434408686	31.14900386040466	13.863185189496077
14	14.23887951269666	35.28034814771706	33.889438527743835	16.591333811842453
15	14.770412617121572	27.3144645547602	43.93101709053522	13.984105737583011
16	15.537967112197457	26.122718190459565	42.03379309006964	16.305521607273338
17	15.644015079503642	25.659081650468487	34.98871623762504	23.708187032402826
18	17.62788802886574	27.015719671251304	37.98974438560075	17.366647914282204
19	22.289472152709074	27.17220508642263	33.96768123532949	16.570641525538807
20	23.70042742503896	26.27791033773691	34.87684856479596	15.144813672428176
21	18.89529056496408	28.66463623607312	35.53964836045962	16.900424838503174
22	18.369577166062065	26.287609846941745	34.19723628651057	21.14557670048562
23	17.203049525694	29.197462608392016	34.351135165893936	19.248352700020046
24	16.795023505143973	26.11107877941376	39.92511978893868	17.168777926503587
25	15.545726719561323	29.62941408498063	36.60659437299139	18.21826482246665
26	15.263147684727151	32.54831972168875	34.26965928857334	17.918873305010766
27	16.382471046965026	33.09019896926549	33.96186152980659	16.565468453962893
28	14.228533369544834	30.221084146475523	39.15497875807484	16.395403725904803
29	15.47524361933953	28.00571624409138	37.422646414091446	19.096393722477643
30	15.778514940477345	31.74002728795256	35.26094912930739	17.220508642262704
31	20.839718843559847	30.194572154648974	31.97540204465654	16.990306957134635
32	20.375435669621783	29.966957005308863	32.92466067883632	16.732946646233035
33	19.643446041630295	31.201381210110767	31.66307784826088	17.492094899998058
34	16.1322237094803	29.79624564330378	33.70126804917005	20.370262598045873
35	17.624008225183807	29.55763771686486	33.0190692350967	19.79928482285463
36	20.53127445084612	30.748090813271517	32.34850983206917	16.3721249038132
37	18.034620781521788	32.36014924311497	33.39411692435029	16.21111305101295
38	17.75074847879364	32.2062503637316	31.195561504587864	18.847439652886898
39	20.034659579558607	30.65109572122317	30.82374698506922	18.490497714148997
40	20.60757725659082	29.031277684015855	31.357219991335107	19.003925068058223
41	16.4717065316495	28.640710780034528	31.615226936183692	23.272355752132277
42	18.52606258123339	29.577683369221518	31.233712907460216	20.662541142084876
43	20.839072209612862	27.33515684106384	31.487840048626875	20.337930900696424
44	17.851623374523914	29.7425750257037	32.46813711226212	19.937664487510265
45	17.076309272084167	32.89814868700977	29.76779374963627	20.257748291269795
46	18.109630319372506	33.02941537824853	30.33812489088052	18.522829411498446
47	17.511493918407727	29.018991639023067	32.65436768899493	20.81514675357427
48	18.16782737460151	28.053567156168562	34.13386615970565	19.64473930952427
49	18.71099989007223	27.544019605941273	34.1603781515322	19.5846023524543
50	16.78338409409817	30.19586542254295	33.09472540689441	19.926025076464466
51	16.740059619649912	28.94204219933138	30.985405471816456	23.33249270920225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	352.5
2	465.0
3	2572.5
4	4680.0
5	3991.5
6	3303.0
7	2499.0
8	1695.0
9	1641.5
10	1588.0
11	1516.5
12	1445.0
13	1467.0
14	1489.0
15	1395.0
16	1301.0
17	1255.5
18	1210.0
19	1223.5
20	1237.0
21	1275.5
22	1314.0
23	1260.5
24	1207.0
25	1625.0
26	2473.5
27	2904.0
28	3341.5
29	3779.0
30	4062.0
31	4345.0
32	4807.0
33	5269.0
34	5705.0
35	6141.0
36	6240.5
37	6340.0
38	7029.0
39	7718.0
40	9203.0
41	10688.0
42	12297.0
43	13906.0
44	15069.5
45	16233.0
46	15248.5
47	14264.0
48	13040.5
49	11817.0
50	10188.0
51	8559.0
52	7434.0
53	6309.0
54	5279.5
55	4250.0
56	3615.0
57	2980.0
58	2686.5
59	2393.0
60	1957.0
61	1521.0
62	1223.5
63	926.0
64	703.0
65	480.0
66	355.0
67	230.0
68	196.0
69	162.0
70	126.0
71	90.0
72	65.0
73	40.0
74	40.0
75	27.5
76	15.0
77	13.5
78	12.0
79	12.5
80	13.0
81	9.0
82	5.0
83	3.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	154647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.412798179078806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98461881408826	36.6589717226975
2	8.394170753455194	7.791939061216836
3	3.4329023629068214	4.779918136142311
4	1.8488074008024966	3.432332990617341
5	1.090893892108783	2.5315719024617356
6	0.7857779759251002	2.1882092766106034
7	0.5781876950512705	1.878471616002897
8	0.43189924208649133	1.6036521885325936
9	0.4193602318323673	1.7517313623930628
>10	3.969293357111012	31.55250344332577
>50	0.03901025412394115	1.2098521148163237
>100	0.02229157378510923	2.1177261763888082
>500	0.0	0.0
>1k	0.0027864467231386537	2.503120008794222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2779	1.7969957386822895	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1092	0.7061242701119324	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	325	0.21015603277140843	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	285	0.18429067489185047	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	271	0.17523779963400518	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	271	0.17523779963400518	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	256	0.16553829042917095	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	253	0.1635983885882041	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	224	0.1448460041255246	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	215	0.13902629860262405	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	189	0.12221381598091137	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	177	0.11445420861704399	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	165	0.10669460125317658	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	161	0.10410806546522079	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18235077305088362	0.0
2	0.0	0.0	0.0	0.5819705522900541	0.0
3	0.0	0.0	0.0	0.762381423499971	0.0
4	0.0	0.0	0.0	1.0359075830762963	0.0
5	0.0	0.0	0.0	1.7588443358099413	0.0
6	0.0	0.0	0.0	2.206315027126294	0.0
7	0.0	0.0	0.0	2.54709111718947	0.0
8	0.0	0.0	0.0	3.374135935388336	0.0
9	0.0	0.0	0.0	3.6463688270706838	0.0
10	0.0	0.0	0.0	4.45466126080687	0.0
11	0.0	0.0	0.0	5.244847944027366	0.0
12	0.0	0.0	0.0	6.145609032182971	0.0
13	0.0	0.0	0.0	6.452760157002722	0.0
14	0.0	0.0	0.0	6.603425866651148	0.0
15	0.0	0.0	0.0	6.834920819673191	0.0
16	0.0	0.0	0.0	7.308903502816091	0.0
17	0.0	0.0	0.0	7.849489482498853	0.0
18	0.0	0.0	0.0	8.725678480668877	0.0
19	0.0	0.0	0.0	9.101372803869458	0.0
20	0.0	0.0	0.0	9.55595646860269	0.0
21	0.0	0.0	0.0	10.012480035176887	0.0
22	0.0	0.0	0.0	10.49034252200172	0.0
23	0.0	0.0	0.0	10.994070366706111	0.0
24	0.0	0.0	0.0	11.43701462039354	0.0
25	0.0	0.0	0.0	11.755158522312104	0.0
26	0.0	0.0	0.0	12.116626898678927	0.0
27	0.0	0.0	0.0	12.453523185060169	0.0
28	0.0	0.0	0.0	12.79171273933539	0.0
29	0.0	0.0	0.0	13.19844549199144	0.0
30	0.0	0.0	0.0	13.656908960406604	0.0
31	0.0	0.0	0.0	14.064288347009642	0.0
32	0.0	0.0	0.0	14.520165279636851	0.0
33	0.0	0.0	0.0	14.900386040466353	0.0
34	0.0	0.0	0.0	15.311645230751324	0.0
35	0.0	0.0	0.0	15.746183243127899	0.0
36	0.0	0.0	0.0	16.213052952853918	0.0
37	0.0	0.0	0.0	16.669576519428116	0.0
38	0.0	0.0	0.0	17.10993423732759	0.0
39	0.0	0.0	0.0	17.594909697569303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	35	1.202825E-7	45.0	5
TCGCAGG	25	3.875107E-5	45.0	2
TTAATCG	20	7.0143363E-4	45.0	20
CTCAACC	20	7.0143363E-4	45.0	36
TATGGGT	40	6.7502697E-9	45.0	4
AGCCATA	20	7.0143363E-4	45.0	45
CGTTGAG	25	3.875107E-5	45.0	25
GGGTACT	20	7.0143363E-4	45.0	7
GGGTACC	25	3.875107E-5	45.0	7
TACGGTT	25	3.875107E-5	45.0	33
TGCGGGC	40	6.7502697E-9	45.0	4
TCGATGG	20	7.0143363E-4	45.0	2
GACTTAT	20	7.0143363E-4	45.0	9
GAAAGGC	20	7.0143363E-4	45.0	4
CTACGAA	20	7.0143363E-4	45.0	11
GGTATGC	25	3.875107E-5	45.0	8
GAACGTT	25	3.875107E-5	45.0	22
CGAATAT	20	7.0143363E-4	45.0	14
GCGATGG	45	3.8016879E-10	45.0	9
CGTACGG	25	3.875107E-5	45.0	31
>>END_MODULE