##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406437
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26679411569321	31.0	31.0	33.0	28.0	34.0
2	31.322561676225344	31.0	31.0	34.0	28.0	34.0
3	30.66168926549502	31.0	31.0	34.0	27.0	34.0
4	35.002930343448064	35.0	35.0	37.0	32.0	37.0
5	34.96898658340653	35.0	35.0	37.0	32.0	37.0
6	35.01760420434163	35.0	35.0	37.0	32.0	37.0
7	32.10615667372803	35.0	35.0	37.0	17.0	37.0
8	33.59237963079149	36.0	35.0	37.0	19.0	37.0
9	36.58016371540977	39.0	35.0	39.0	32.0	39.0
10	36.185645007713376	37.0	35.0	39.0	32.0	39.0
11	36.15523931138159	37.0	35.0	39.0	32.0	39.0
12	36.268373696292414	38.0	35.0	39.0	32.0	39.0
13	32.74604182197979	37.0	34.0	39.0	11.0	39.0
14	35.23192032221476	38.0	34.0	40.0	18.0	41.0
15	36.489143458887845	38.0	34.0	40.0	30.0	41.0
16	37.093620905577005	38.0	36.0	40.0	32.0	41.0
17	37.19571790954072	39.0	36.0	40.0	32.0	41.0
18	37.0848421772624	39.0	36.0	40.0	32.0	41.0
19	37.017931438328695	39.0	36.0	40.0	32.0	41.0
20	36.90405647123662	38.0	35.0	40.0	32.0	41.0
21	36.8729938465248	38.0	35.0	40.0	32.0	41.0
22	36.918678663605924	38.0	35.0	40.0	32.0	41.0
23	36.81499469782525	38.0	35.0	40.0	32.0	41.0
24	36.673627155008035	38.0	35.0	40.0	31.0	41.0
25	36.536562862141	38.0	35.0	40.0	31.0	41.0
26	36.36732138068138	38.0	35.0	40.0	31.0	41.0
27	36.37140319409896	38.0	35.0	40.0	31.0	41.0
28	36.308229811754344	38.0	35.0	40.0	31.0	41.0
29	36.263996634164705	38.0	35.0	40.0	30.0	41.0
30	36.03190900434754	38.0	35.0	40.0	30.0	41.0
31	35.91520457045003	38.0	35.0	40.0	30.0	41.0
32	35.71318064054208	38.0	34.0	40.0	29.0	41.0
33	35.36697938425881	38.0	34.0	40.0	27.0	41.0
34	35.13235753634635	38.0	34.0	40.0	25.0	41.0
35	34.821679620703826	38.0	34.0	40.0	24.0	41.0
36	34.56235529737696	38.0	34.0	40.0	23.0	41.0
37	34.47198704842325	38.0	33.0	40.0	22.0	41.0
38	34.35991309846298	38.0	33.0	40.0	21.0	41.0
39	34.262692619028286	38.0	33.0	40.0	21.0	41.0
40	34.19618046585326	38.0	33.0	40.0	21.0	41.0
41	34.1573454188472	38.0	33.0	40.0	21.0	41.0
42	34.10985220341652	38.0	33.0	40.0	20.0	41.0
43	34.07368423642532	38.0	33.0	40.0	20.0	41.0
44	33.94415616688441	38.0	33.0	40.0	19.0	41.0
45	33.92142201620423	38.0	33.0	40.0	20.0	41.0
46	33.76809444022075	37.0	33.0	40.0	19.0	41.0
47	33.55900668492288	37.0	32.0	40.0	18.0	41.0
48	33.43740112243718	37.0	32.0	40.0	18.0	41.0
49	33.371452402217315	37.0	32.0	40.0	18.0	41.0
50	33.19574497400581	37.0	32.0	40.0	17.0	41.0
51	31.209764366925256	35.0	28.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	12.0
10	21.0
11	10.0
12	6.0
13	6.0
14	8.0
15	17.0
16	47.0
17	90.0
18	190.0
19	418.0
20	745.0
21	1271.0
22	1890.0
23	3084.0
24	4984.0
25	7151.0
26	8674.0
27	8875.0
28	8822.0
29	9503.0
30	11198.0
31	13955.0
32	18165.0
33	23913.0
34	32689.0
35	40256.0
36	46247.0
37	59588.0
38	63621.0
39	40976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.1528305739881	23.480637835630123	28.0493655843341	16.317166006047678
2	33.61062108026582	27.647089216778987	26.499801937323618	12.242487765631575
3	28.091684566119717	26.3088744381048	32.810004994624016	12.789436001151469
4	22.76884240361975	24.749961248606795	38.03221655508725	14.448979792686197
5	22.550112317530147	29.275385853158053	33.672623309393586	14.501878519918215
6	19.204206309957016	39.54536619451477	31.98872149927295	9.261705996255262
7	70.06916201034846	13.062295017431977	14.248948791571634	2.6195941806479235
8	75.60778177183671	6.059979775463356	14.227051178903496	4.105187273796431
9	70.64317471096382	6.704114044735101	16.19512987252637	6.457581371774715
10	34.14772769211464	24.675656989890193	26.476182040513045	14.70043327748212
11	24.55854166820442	26.625282639129804	30.98339964127282	17.83277605139296
12	22.64385378299712	22.865536356188045	35.63725743473158	18.853352426083255
13	17.974987513439967	33.646789047257016	34.10417850737015	14.274044931932869
14	17.62560987311687	29.671265165326975	34.85607855584014	17.847046405716014
15	16.467988888806875	27.92609924785391	39.792883029842265	15.81302883349695
16	17.287304059423725	26.654315428959467	37.28745168377879	18.770928827838016
17	17.197007162241626	27.663327895836254	34.105162669737254	21.034502272184866
18	17.954566144322488	27.508568363608628	36.37218068236898	18.164684809699907
19	19.761488250331542	30.184013758589895	32.53074892295731	17.52374906812126
20	20.543159210406532	27.718687028986043	35.02092575233062	16.717228008276805
21	18.58295381572052	29.91361514822716	34.36252112873582	17.14090990731651
22	17.65857931241496	26.27467479584782	33.272315266572676	22.794430625164537
23	17.24031030639435	29.407263610350437	33.738316147397995	19.61410993585722
24	18.02001294173513	26.905522873163612	35.533920386185315	19.540543798915948
25	16.513260357693813	30.516660638672167	32.855768544694506	20.114310458939517
26	16.651535170272393	30.674126617409343	33.62390727222177	19.050430940096497
27	18.305666068788028	30.291779537788145	32.61932353599697	18.783230857426858
28	15.789162896094597	28.753287717407616	36.067582429749265	19.389966956748523
29	18.431884892369542	26.19938637476411	34.518756904514106	20.849971828352242
30	17.704834943669006	30.614338753607573	33.672623309393586	18.00820299332984
31	18.67743340296282	29.379214982887873	33.13969938760496	18.803652226544333
32	19.493304005294792	29.02516257132102	33.31340404539941	18.16812937798478
33	17.637419821522155	30.337051006675082	31.976665510275886	20.048863661526877
34	17.944724520651416	27.78314966403157	34.483573099890016	19.78855271542699
35	19.27998681222428	27.94578249519606	32.43799161985744	20.33623907272222
36	19.599593540942383	29.5669439544136	31.479171433703137	19.354291070940885
37	16.858455307956707	29.873264491175753	35.252942030376175	18.01533817049137
38	17.515629728592625	30.202466802973156	32.13511565138016	20.146787817054058
39	18.02936248422265	28.498881745510374	33.50506966639356	19.966686103873418
40	20.159335887234676	28.2808897811961	32.63039536262692	18.929378968942295
41	18.11990542199652	27.553839832495562	31.525181024365402	22.801073721142515
42	20.093397008638487	26.577058683141548	31.84232734716573	21.487216961054234
43	18.879924809995153	27.084886464568925	32.17300590251379	21.862182822922126
44	17.77446443114185	28.536771996644006	31.3775566692993	22.311206902914844
45	17.807433870439944	28.799789389253437	31.31309403425377	22.079682706052843
46	20.266363544657597	29.799206273050928	30.445554907648663	19.48887527464281
47	17.218658734317987	27.098910778300205	34.580513093050094	21.101917394331718
48	17.99245639545612	27.120562350376566	33.303070340544785	21.58391091362253
49	18.48724402551933	25.594372559584883	34.74806673605011	21.170316678845676
50	17.546630843156503	25.92062238428096	33.81754121795014	22.7152055546124
51	17.484628614028743	25.7619262025849	30.964208475114223	25.789236708272128
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	260.0
1	396.0
2	532.0
3	4428.0
4	8324.0
5	7364.0
6	6404.0
7	5054.5
8	3705.0
9	3688.0
10	3671.0
11	3665.0
12	3659.0
13	3692.5
14	3726.0
15	3628.5
16	3531.0
17	3419.0
18	3307.0
19	3200.0
20	3093.0
21	3002.5
22	2912.0
23	2946.5
24	2981.0
25	3477.5
26	4662.0
27	5350.0
28	6328.5
29	7307.0
30	8178.0
31	9049.0
32	10556.0
33	12063.0
34	13238.0
35	14413.0
36	15986.0
37	17559.0
38	19025.5
39	20492.0
40	23961.5
41	27431.0
42	31509.0
43	35587.0
44	43592.5
45	51598.0
46	48076.5
47	44555.0
48	40967.0
49	37379.0
50	31737.0
51	26095.0
52	21712.5
53	17330.0
54	14454.0
55	11578.0
56	9612.5
57	7647.0
58	6251.5
59	4856.0
60	3817.5
61	2779.0
62	2182.0
63	1585.0
64	1179.5
65	774.0
66	572.0
67	370.0
68	285.0
69	200.0
70	163.0
71	126.0
72	113.5
73	101.0
74	76.0
75	48.0
76	45.0
77	29.0
78	13.0
79	13.5
80	14.0
81	10.0
82	6.0
83	4.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	406437.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.72817023755988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0431825695892	44.35345092262124
2	8.80925159572326	9.642284419924774
3	3.1402682018126398	5.155833982211947
4	1.5882882642702976	3.4769644205321337
5	0.9849728231108505	2.6952880171290294
6	0.7281132816993517	2.390898457984231
7	0.552830358166855	2.117877576797279
8	0.44854288123871944	1.9638344930622595
9	0.3967917936145402	1.9544119946822955
>10	2.257897587616879	19.45786990713884
>50	0.03221085796907007	1.1777228917755884
>100	0.012796094261567203	1.5191828402924985
>500	0.0026474677782552833	0.9521757970550503
>1k	0.0022062231485460693	3.142204278792877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4914	1.2090434679913493	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	2154	0.5299714346872947	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	2041	0.5021688478165128	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2028	0.49897032012341397	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	1875	0.4613261095815588	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	756	0.1860066873832845	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	699	0.1719823736520051	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	679	0.1670615618164685	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	654	0.16091054702204768	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	599	0.14737831447432198	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.13679856902791823	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	497	0.12228217411308517	Illumina Single End Adapter 2 (95% over 21bp)
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	484	0.11908364641998637	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4604059177683136E-4	0.0	0.0	0.2701525697709608	0.0
2	2.4604059177683136E-4	0.0	0.0	0.8328474031645741	0.0
3	2.4604059177683136E-4	0.0	0.0	1.1229292608694583	0.0
4	2.4604059177683136E-4	0.0	0.0	1.7035850574627802	0.0
5	2.4604059177683136E-4	0.0	0.0	3.1896702317948415	0.0
6	2.4604059177683136E-4	0.0	0.0	3.9351732248786404	0.0
7	2.4604059177683136E-4	0.0	0.0	4.632206221382404	0.0
8	2.4604059177683136E-4	0.0	0.0	6.04177277167187	0.0
9	2.4604059177683136E-4	0.0	0.0	6.455859087632278	0.0
10	2.4604059177683136E-4	0.0	0.0	7.995089029788135	0.0
11	2.4604059177683136E-4	0.0	0.0	9.503563897971887	0.0
12	2.4604059177683136E-4	0.0	0.0	11.143178401572692	0.0
13	2.4604059177683136E-4	0.0	0.0	11.65272846714251	0.0
14	2.4604059177683136E-4	0.0	0.0	11.886220988738723	0.0
15	2.4604059177683136E-4	0.0	0.0	12.340165880566976	0.0
16	2.4604059177683136E-4	0.0	0.0	13.166370187753575	0.0
17	2.4604059177683136E-4	0.0	0.0	14.143397377699372	0.0
18	2.4604059177683136E-4	0.0	0.0	15.269771206853706	0.0
19	2.4604059177683136E-4	0.0	0.0	15.983781004190071	0.0
20	2.4604059177683136E-4	0.0	0.0	16.63923314068355	0.0
21	2.4604059177683136E-4	0.0	0.0	17.53309861060878	0.0
22	2.4604059177683136E-4	0.0	0.0	18.480600929541357	0.0
23	2.4604059177683136E-4	0.0	0.0	19.47632720446219	0.0
24	2.4604059177683136E-4	0.0	0.0	20.192059285940996	0.0
25	2.4604059177683136E-4	0.0	0.0	20.831026702785426	0.0
26	2.4604059177683136E-4	0.0	0.0	21.409468134052755	0.0
27	2.4604059177683136E-4	0.0	0.0	22.006608650295124	0.0
28	2.4604059177683136E-4	0.0	0.0	22.656401853177737	0.0
29	2.4604059177683136E-4	0.0	0.0	23.328092668728488	0.0
30	2.4604059177683136E-4	0.0	0.0	24.06719860642609	0.0
31	2.4604059177683136E-4	0.0	0.0	24.768660333581835	0.0
32	2.4604059177683136E-4	0.0	0.0	25.43075556605329	0.0
33	2.4604059177683136E-4	0.0	0.0	26.09211267674941	0.0
34	2.4604059177683136E-4	0.0	0.0	26.73354049951161	0.0
35	2.4604059177683136E-4	0.0	0.0	27.406461518021242	0.0
36	2.4604059177683136E-4	0.0	0.0	28.106693042218104	0.0
37	2.4604059177683136E-4	0.0	0.0	28.781828426053732	0.0
38	2.4604059177683136E-4	0.0	0.0	29.48402827498481	0.0
39	2.4604059177683136E-4	0.0	0.0	30.17884690616258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCT	65	0.0	45.000004	11
CCGGCGC	30	2.1620253E-6	45.000004	21
TCGATTG	20	7.027898E-4	45.000004	1
TACGGCA	30	2.1620253E-6	45.000004	7
CGACAAT	30	2.1620253E-6	45.000004	20
ACGGGCG	20	7.027898E-4	45.000004	5
GCGATGC	30	2.1620253E-6	45.000004	9
CTACCGG	20	7.027898E-4	45.000004	2
TCGAAAG	20	7.027898E-4	45.000004	1
TGCGTAG	40	6.7957444E-9	45.000004	1
CGTGGGC	35	1.2093369E-7	45.0	4
TCGCATG	35	1.2093369E-7	45.0	1
ACCGGCG	25	3.8863407E-5	45.0	20
GCGCGAC	25	3.8863407E-5	45.0	9
CGCGACC	25	3.8863407E-5	45.0	10
CTCGGGC	25	3.8863407E-5	45.0	4
TAGGCGA	25	3.8863407E-5	45.0	6
TGCACGG	25	3.8863407E-5	45.0	2
CGCTTGG	25	3.8863407E-5	45.0	2
GTTGCGG	25	3.8863407E-5	45.0	2
>>END_MODULE