FastQCFastQC Report
Sat 18 Jun 2016
SRR3549418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247027
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38591.5621774138049687No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.6436543373801245No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG13370.541236383067438No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC11830.4788950195727592RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC11820.47849020552409255No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6660.2696061564120521No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5390.21819477223137554No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.20200221028470572No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4360.17649892521870078No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4110.16637857400203215No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.1522100822986961No Hit
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT3520.1424945451306942Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT3400.13763677654669326RNA PCR Primer, Index 41 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTC3400.13763677654669326No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC3270.13237419391402558Illumina PCR Primer Index 11 (96% over 25bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2800.11334793362668859No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT2760.11172867743202158RNA PCR Primer, Index 41 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG207.0225215E-445.0000046
GGTACCT406.7775545E-945.0000048
CATGCGG207.0225215E-445.0000042
GACGTTT207.0225215E-445.00000428
CGGTTTA1350.045.0000041
TTTCGAC207.0225215E-445.00000424
TTATGCC207.0225215E-445.00000423
CTACGGG406.7775545E-945.0000043
CGGGCTC207.0225215E-445.0000046
CCATAGT207.0225215E-445.00000414
TCAGACG207.0225215E-445.00000422
GGGCCGC207.0225215E-445.0000047
GCTAACC207.0225215E-445.00000433
AAGGCGA207.0225215E-445.0000046
TCGACGT207.0225215E-445.00000426
TTGGGCG207.0225215E-445.0000045
ATGGGCC351.206754E-745.05
TAACGGG502.1827873E-1145.03
ACGGGGC253.8818864E-545.05
ACGGGCT253.8818864E-545.05
Produced by FastQC (version 0.11.5)