##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373442
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43296951066029	31.0	31.0	34.0	30.0	34.0
2	31.514730533791056	31.0	31.0	34.0	30.0	34.0
3	30.927129246308663	31.0	31.0	34.0	27.0	34.0
4	35.17859265963657	37.0	35.0	37.0	32.0	37.0
5	35.12201359247219	35.0	35.0	37.0	33.0	37.0
6	35.173151386292915	36.0	35.0	37.0	32.0	37.0
7	32.05245258969264	36.0	35.0	37.0	11.0	37.0
8	33.59836065573771	37.0	35.0	37.0	19.0	37.0
9	36.6327863496875	39.0	35.0	39.0	32.0	39.0
10	36.291635648909335	38.0	35.0	39.0	32.0	39.0
11	36.27792535387021	38.0	35.0	39.0	32.0	39.0
12	36.37848447683978	38.0	35.0	39.0	32.0	39.0
13	32.813995212107905	37.0	34.0	39.0	10.0	39.0
14	35.371771252296206	39.0	34.0	40.0	18.0	41.0
15	36.6515282158943	39.0	35.0	40.0	30.0	41.0
16	37.22691877185748	39.0	36.0	40.0	32.0	41.0
17	37.359249896905006	39.0	36.0	40.0	32.0	41.0
18	37.250062928111994	39.0	36.0	40.0	32.0	41.0
19	37.22699910561747	39.0	36.0	40.0	32.0	41.0
20	37.13312910706348	39.0	35.0	40.0	32.0	41.0
21	37.107874850713095	39.0	35.0	40.0	32.0	41.0
22	37.147147348182585	39.0	35.0	40.0	32.0	41.0
23	37.03912254111749	39.0	35.0	40.0	32.0	41.0
24	36.90559444304604	39.0	35.0	40.0	32.0	41.0
25	36.72278426100974	38.0	35.0	40.0	31.0	41.0
26	36.57111412213945	38.0	35.0	40.0	31.0	41.0
27	36.58795475602637	38.0	35.0	40.0	31.0	41.0
28	36.52285495471854	38.0	35.0	40.0	31.0	41.0
29	36.49326803091243	38.0	35.0	40.0	31.0	41.0
30	36.32283995908334	38.0	35.0	40.0	30.0	41.0
31	36.21336378875434	38.0	35.0	40.0	30.0	41.0
32	36.01851425388681	38.0	35.0	40.0	30.0	41.0
33	35.73352220692905	38.0	35.0	40.0	29.0	41.0
34	35.458885181634635	38.0	34.0	40.0	27.0	41.0
35	35.23071320312123	38.0	34.0	40.0	25.0	41.0
36	35.02101263382266	38.0	34.0	40.0	24.0	41.0
37	34.91355819645353	38.0	34.0	40.0	24.0	41.0
38	34.75954498958339	38.0	34.0	40.0	23.0	41.0
39	34.59099137215418	38.0	34.0	40.0	23.0	41.0
40	34.520838577342666	38.0	33.0	40.0	22.0	41.0
41	34.47314977961772	38.0	33.0	40.0	22.0	41.0
42	34.51123869302328	38.0	34.0	40.0	22.0	41.0
43	34.501652197663894	38.0	34.0	40.0	22.0	41.0
44	34.36110292896889	38.0	33.0	40.0	21.0	41.0
45	34.334766844650574	38.0	33.0	40.0	22.0	41.0
46	34.23362128523305	38.0	33.0	40.0	21.0	41.0
47	34.057792106940305	38.0	33.0	40.0	20.0	41.0
48	33.94130012157176	38.0	33.0	40.0	20.0	41.0
49	33.86695390448852	37.0	33.0	40.0	20.0	41.0
50	33.712450661682404	37.0	33.0	40.0	19.0	41.0
51	31.92517713594079	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	10.0
11	10.0
12	6.0
13	5.0
14	7.0
15	18.0
16	43.0
17	87.0
18	173.0
19	373.0
20	602.0
21	1039.0
22	1643.0
23	2558.0
24	3975.0
25	5949.0
26	7389.0
27	7565.0
28	7595.0
29	7999.0
30	9580.0
31	11939.0
32	15654.0
33	20943.0
34	28213.0
35	34877.0
36	40523.0
37	55158.0
38	61993.0
39	47496.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.0265476298863	23.776115166478327	27.21841678225802	15.97892042137735
2	33.72866469224137	27.365963121448576	25.986632462336857	12.9187397239732
3	29.223547431729692	26.191751329523726	31.527251889182256	13.057449349564324
4	24.349430433641636	25.255863025583626	36.1030628584894	14.291643682285335
5	23.154599643318104	30.146314554870635	32.69557253870749	14.003513263103775
6	20.616320606680556	39.88785407104718	30.506745358047567	8.989079964224699
7	71.89817963699852	13.38467553194338	12.375683506407956	2.3414613246501466
8	78.80661521735638	5.847762169225743	12.340604431210203	3.005018182207679
9	73.54555727529308	6.8272984827630525	14.14891736869447	5.478226873249394
10	34.773003572174524	27.35953642064899	24.42842529763658	13.439034709539902
11	25.523106667166523	28.455021127778878	29.489987735712642	16.53188446934196
12	23.828867668874953	24.188227355251954	34.28243207780592	17.700472898067172
13	19.173526277172893	33.423931962660866	32.601314260313515	14.80122749985272
14	18.427761205220623	29.832745111690706	33.7749904938384	17.964503189250273
15	17.479822837281294	28.835535370954524	38.2827855463499	15.40185624541428
16	18.924223841988848	27.698277108627313	35.435757092132114	17.94174195725173
17	18.784978657997762	28.434937687780167	33.243181002672436	19.53690265154964
18	19.6922145875397	28.905157962950067	34.2235206538097	17.179106795700537
19	21.493297486624428	30.121678868472213	31.27982390839809	17.105199736505266
20	21.900589649798363	28.010507655807327	34.08320435301867	16.005698341375634
21	20.346399173097833	30.171485799669025	33.40063517226236	16.081479854970784
22	20.130569137911642	26.509605239903383	32.670669073109075	20.689156549075893
23	18.560579688412123	30.005194916479667	33.26299666347117	18.17122873163704
24	18.917529361989278	28.097000337401788	34.488086503392765	18.49738379721617
25	17.95325646285099	31.18235227960433	32.413065482725564	18.451325774819118
26	17.89247058445488	30.843343812426028	33.02494095468641	18.239244648432688
27	18.83719560199442	31.113265246008755	31.98408320435302	18.06545594764381
28	16.858039534921083	29.178024967732608	34.6187627529844	19.345172744361907
29	18.62324002120811	27.46289919184238	33.240770989872594	20.673089797076923
30	18.852726795593426	30.147921230070533	32.906582548293976	18.092769426042064
31	19.397121909158585	29.304952308524484	31.687919409171972	19.61000637314496
32	19.765050529935035	29.478205450913396	32.6031887147134	18.153555304438175
33	19.715511377938206	29.923522260484898	30.922338676420967	19.43862768515593
34	17.306569694892378	30.261727390063246	31.727818509969417	20.70388440507495
35	18.478103694817403	29.722420081297763	32.09628268914584	19.703193534738997
36	19.134966072375363	30.539146641245495	30.515046513247036	19.810840773132107
37	18.263612555631127	31.43701029878803	32.41493993712544	17.884437208455395
38	17.62844029327178	31.877774861959825	30.22718387326546	20.26660097150294
39	18.74079509000059	31.006956903615556	31.785658817165718	18.466589189218137
40	19.12827159237579	31.30204958199667	30.572886820443333	18.996792005184204
41	17.404576882086108	30.738106586832757	31.088094001210365	20.76922252987077
42	18.365904210024585	29.530957953310015	31.679350474772523	20.423787361892877
43	19.37489623556001	28.85535103175326	30.941083220419767	20.828669512266966
44	18.010025653247357	30.181125850868405	30.04830736767691	21.760541128207326
45	17.86140819725687	30.671161786837047	29.969580282881946	21.49784973302414
46	19.296436929965026	31.955698609154837	28.863919966152707	19.883944494727427
47	17.472325019681772	29.5234601357105	32.1546585547421	20.849556289865628
48	18.047514741244957	29.460532023714524	31.287589505197595	21.20436372984292
49	17.972536565249758	29.218191847730036	32.43716561072402	20.372105976296183
50	17.429748126884494	29.385821626919306	31.58455663797859	21.599873608217607
51	17.27015172369471	29.205874004530823	30.048575146876892	23.475399124897574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	180.0
1	371.0
2	562.0
3	3495.0
4	6428.0
5	5793.5
6	5159.0
7	4133.5
8	3108.0
9	3040.0
10	2972.0
11	3015.0
12	3058.0
13	3004.0
14	2950.0
15	2957.0
16	2964.0
17	2804.5
18	2645.0
19	2530.0
20	2415.0
21	2603.5
22	2792.0
23	3006.5
24	3221.0
25	3788.0
26	5095.5
27	5836.0
28	6789.5
29	7743.0
30	8888.5
31	10034.0
32	11289.5
33	12545.0
34	14276.0
35	16007.0
36	17299.5
37	18592.0
38	20542.5
39	22493.0
40	24160.0
41	25827.0
42	27994.0
43	30161.0
44	35499.0
45	40837.0
46	37537.5
47	34238.0
48	32332.0
49	30426.0
50	27013.0
51	23600.0
52	20906.0
53	18212.0
54	15510.5
55	12809.0
56	10577.0
57	8345.0
58	6860.0
59	5375.0
60	4372.0
61	3369.0
62	2696.0
63	2023.0
64	1466.0
65	909.0
66	716.5
67	524.0
68	406.0
69	288.0
70	239.0
71	190.0
72	146.0
73	102.0
74	83.0
75	45.5
76	27.0
77	28.0
78	29.0
79	24.0
80	19.0
81	11.5
82	4.0
83	3.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	373442.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.99086763719673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07108707775751	41.900455092667045
2	9.024657047701702	9.564488141717108
3	3.4465203912504307	5.479023175849532
4	1.8880651314340997	4.002008378809233
5	1.281413761473615	3.3951613511365357
6	0.8994936169831592	2.8598968318834754
7	0.7082114938349134	2.6270119070263083
8	0.5964615480776189	2.528561195588684
9	0.5038604895421136	2.4030004058045358
>10	2.5454093452859485	20.412697841813895
>50	0.018902338186254107	0.6935117296226562
>100	0.012933178759015111	1.4545031258295793
>500	4.97429952269812E-4	0.14471225372969174
>1k	0.00248714976134906	2.5349685685217582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3502	0.9377627583399831	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	1728	0.46272245757038577	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1697	0.454421302370917	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.39658099517461876	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	1209	0.32374505277928034	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	549	0.14701078079059132	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTC	465	0.1245173279920309	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	424	0.11353838079273355	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	424	0.11353838079273355	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	396	0.1060405631932134	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6777919998286214E-4	0.0	0.0	0.12103619839225369	0.0
2	2.6777919998286214E-4	0.0	0.0	0.39711655357458453	0.0
3	2.6777919998286214E-4	0.0	0.0	0.5323450495659299	0.0
4	2.6777919998286214E-4	0.0	0.0	0.9005414495423654	0.0
5	2.6777919998286214E-4	0.0	0.0	1.866688803080532	0.0
6	2.6777919998286214E-4	0.0	0.0	2.2260484894575328	0.0
7	2.6777919998286214E-4	0.0	0.0	2.600403811033574	0.0
8	2.6777919998286214E-4	0.0	0.0	3.3338510397866337	0.0
9	2.6777919998286214E-4	0.0	0.0	3.5245098301744315	0.0
10	2.6777919998286214E-4	0.0	0.0	4.552246399708657	0.0
11	2.6777919998286214E-4	0.0	0.0	5.249543436464029	0.0
12	2.6777919998286214E-4	0.0	0.0	6.16936498840516	0.0
13	2.6777919998286214E-4	0.0	0.0	6.422148553188983	0.0
14	2.6777919998286214E-4	0.0	0.0	6.546398101981031	0.0
15	2.6777919998286214E-4	0.0	0.0	6.81953288596355	0.0
16	2.6777919998286214E-4	0.0	0.0	7.255209644335666	0.0
17	2.6777919998286214E-4	0.0	0.0	7.739086658704698	0.0
18	2.6777919998286214E-4	0.0	0.0	8.285891785069703	0.0
19	2.6777919998286214E-4	0.0	0.0	8.656765977045968	0.0
20	2.6777919998286214E-4	0.0	0.0	9.018535676222815	0.0
21	2.6777919998286214E-4	0.0	0.0	9.538830661789515	0.0
22	2.6777919998286214E-4	0.0	0.0	10.107861461753098	0.0
23	2.6777919998286214E-4	0.0	0.0	10.730983660113218	0.0
24	2.6777919998286214E-4	0.0	0.0	11.182459391284322	0.0
25	2.6777919998286214E-4	0.0	0.0	11.598052709657726	0.0
26	2.6777919998286214E-4	0.0	0.0	11.965177992834228	0.0
27	2.6777919998286214E-4	0.0	0.0	12.359348975209002	0.0
28	2.6777919998286214E-4	0.0	0.0	12.804398005580518	0.0
29	2.6777919998286214E-4	0.0	0.0	13.300057304748796	0.0
30	2.6777919998286214E-4	0.0	0.0	13.78982546151745	0.0
31	2.6777919998286214E-4	0.0	0.0	14.329132770282936	0.0
32	2.6777919998286214E-4	0.0	0.0	14.826934303051075	0.0
33	2.6777919998286214E-4	0.0	0.0	15.30733018782033	0.0
34	2.6777919998286214E-4	0.0	0.0	15.810755083788111	0.0
35	2.6777919998286214E-4	0.0	0.0	16.335334536554537	0.0
36	2.6777919998286214E-4	0.0	0.0	16.864466235720673	0.0
37	2.6777919998286214E-4	0.0	0.0	17.414752491685455	0.0
38	2.6777919998286214E-4	0.0	0.0	17.997975589248128	0.0
39	2.6777919998286214E-4	0.0	0.0	18.58253758281072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGCGG	35	1.2089913E-7	45.000004	2
TGTAGCG	30	2.1615506E-6	45.000004	1
ACGTAGG	35	1.2089913E-7	45.000004	2
CACGGGC	20	7.0271624E-4	45.0	4
ATTCGGA	25	3.8857303E-5	45.0	33
CGGGCTT	25	3.8857303E-5	45.0	6
GCGGGCC	20	7.0271624E-4	45.0	5
CTCGAGG	20	7.0271624E-4	45.0	2
TGGGACG	25	3.8857303E-5	45.0	6
CGGTTTA	230	0.0	44.999996	1
CGGTTTT	740	0.0	44.087837	1
CGTTTTT	2005	0.0	42.41895	1
CTCGTCC	125	0.0	41.399998	37
TCCTCGT	125	0.0	41.399998	35
CTCCTCG	125	0.0	41.399998	34
CACGGGA	60	3.6379788E-12	41.250004	4
TCGATGG	55	6.002665E-11	40.909092	2
TACGGCT	155	0.0	40.64516	7
AGCGGGC	45	1.922308E-8	40.0	4
AGGGACG	45	1.922308E-8	40.0	6
>>END_MODULE