FastQCFastQC Report
Sat 18 Jun 2016
SRR3549414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821502
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59860.728665298441148No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.2999384055060122No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG16620.20231234981777282No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC15300.1862442209513793No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC12920.15727289769227587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA207.0321094E-445.0000049
CGACGGT207.0321094E-445.00000428
CCGTGCC207.0321094E-445.00000417
CATAGCG207.0321094E-445.0000041
GCGATCG207.0321094E-445.0000049
CCGCAAC207.0321094E-445.00000412
GTACGAG207.0321094E-445.0000041
CCGACGG253.889831E-545.02
TATCGCG351.211356E-745.01
CGGACGG253.889831E-545.02
ACAGCGG600.044.9999962
CGCACGG302.164743E-644.9999962
TATCGAG302.164743E-644.9999961
CGGTTTT9200.043.5326081
CGGTTTA2550.042.3529361
TACGGGA4150.042.2891544
AAGTGCG700.041.7857131
GACGGGT650.041.5384644
GCACGGG1850.041.3513533
CGTTTTT28850.041.1005171
Produced by FastQC (version 0.11.5)