##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821502
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.612856694201596	31.0	31.0	34.0	30.0	34.0
2	31.751219108413615	31.0	31.0	34.0	30.0	34.0
3	31.362800090565816	31.0	31.0	34.0	28.0	34.0
4	35.40710065221996	37.0	35.0	37.0	33.0	37.0
5	35.28877592507383	37.0	35.0	37.0	33.0	37.0
6	35.3234392612556	37.0	35.0	37.0	33.0	37.0
7	32.17876402005108	37.0	35.0	37.0	11.0	37.0
8	33.76633897422039	37.0	35.0	37.0	19.0	37.0
9	36.80292683401866	39.0	37.0	39.0	32.0	39.0
10	36.53740343906649	38.0	35.0	39.0	32.0	39.0
11	36.433113979028654	38.0	35.0	39.0	32.0	39.0
12	36.596366168311214	39.0	35.0	39.0	32.0	39.0
13	32.921957585008926	38.0	35.0	39.0	8.0	39.0
14	35.43956435894252	39.0	35.0	40.0	18.0	41.0
15	36.79776677354407	39.0	35.0	41.0	30.0	41.0
16	37.39957541186753	39.0	36.0	41.0	32.0	41.0
17	37.53728779722995	39.0	36.0	41.0	32.0	41.0
18	37.46701529637177	39.0	36.0	40.0	32.0	41.0
19	37.51008275086366	39.0	36.0	40.0	32.0	41.0
20	37.445748153991104	39.0	36.0	40.0	32.0	41.0
21	37.40834349764212	39.0	36.0	40.0	32.0	41.0
22	37.46643586990658	39.0	36.0	40.0	33.0	41.0
23	37.38177509001804	39.0	35.0	40.0	32.0	41.0
24	37.29160854142778	39.0	35.0	40.0	32.0	41.0
25	37.13883715438307	39.0	35.0	40.0	32.0	41.0
26	37.01289832526275	39.0	35.0	40.0	32.0	41.0
27	37.01881188359858	39.0	35.0	40.0	32.0	41.0
28	36.94247122952835	39.0	35.0	40.0	31.0	41.0
29	36.920435981896574	39.0	35.0	40.0	31.0	41.0
30	36.78297800857454	39.0	35.0	40.0	31.0	41.0
31	36.667918033066265	39.0	35.0	40.0	31.0	41.0
32	36.54069618820161	39.0	35.0	40.0	30.0	41.0
33	36.345541459424325	39.0	35.0	40.0	30.0	41.0
34	36.1634518236109	39.0	35.0	40.0	30.0	41.0
35	36.02873030132611	39.0	35.0	40.0	29.0	41.0
36	35.886308250010345	38.0	35.0	40.0	29.0	41.0
37	35.78342840309579	38.0	35.0	40.0	27.0	41.0
38	35.65924611260837	38.0	35.0	40.0	27.0	41.0
39	35.480639121998486	38.0	35.0	40.0	26.0	41.0
40	35.42215600205477	38.0	35.0	40.0	26.0	41.0
41	35.357849402679484	38.0	34.0	40.0	25.0	41.0
42	35.33747574564639	38.0	34.0	40.0	25.0	41.0
43	35.32092070378405	38.0	34.0	40.0	25.0	41.0
44	35.18093200016555	38.0	34.0	40.0	24.0	41.0
45	35.14344700317224	38.0	34.0	40.0	24.0	41.0
46	35.048064399112846	38.0	34.0	40.0	24.0	41.0
47	34.842641892533436	38.0	34.0	40.0	23.0	41.0
48	34.76086485486341	38.0	34.0	40.0	23.0	41.0
49	34.66723270302446	38.0	34.0	40.0	23.0	41.0
50	34.521732144291796	38.0	33.0	40.0	23.0	41.0
51	32.657603998529524	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	20.0
11	21.0
12	0.0
13	7.0
14	13.0
15	32.0
16	53.0
17	139.0
18	282.0
19	587.0
20	987.0
21	1629.0
22	2603.0
23	4126.0
24	6389.0
25	9467.0
26	11817.0
27	13338.0
28	14376.0
29	16062.0
30	19434.0
31	24466.0
32	31586.0
33	42130.0
34	57951.0
35	71200.0
36	87679.0
37	125710.0
38	149230.0
39	130141.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.604427012959192	23.75806753970167	29.502545337686335	15.134960109652805
2	30.62536670635008	26.2632349038712	30.00637856024696	13.105019829531761
3	28.935778610398998	25.996650038587855	32.12408490788823	12.943486443124911
4	25.120571830622445	27.23523497203902	33.59005821044867	14.054134986889869
5	24.12264364542022	31.77545520278709	30.06079108754452	14.04111006424817
6	21.84924686732351	41.0252196586253	27.306689454194878	9.818844019856312
7	76.78435353778809	12.765032830108753	8.467538727842415	1.9830749042607323
8	84.58932540638976	4.14362959554572	8.765772937862598	2.5012720602019227
9	79.27333104484225	6.548127697802318	10.289201973945286	3.889339283410144
10	33.107770887958885	42.074151980153424	15.754678625249847	9.063398506637842
11	21.564889677687944	25.37437522976207	36.27952214358577	16.781212948964214
12	24.050580522019423	23.04194025090627	35.39918344690579	17.50829578016852
13	19.637079398467687	32.44836896319181	32.21160751891048	15.70294411943002
14	17.09052442964229	28.93030083919455	34.88390776894031	19.09526696222286
15	16.060946899703225	29.590189676957575	38.03313929850445	16.315724124834755
16	20.369396544378468	29.32616110490297	34.06248554477043	16.241956805948128
17	20.1190015362105	28.38678420746389	32.978860672280774	18.515353584044835
18	19.860207278862376	28.62111108676546	34.063946283758284	17.454735350613877
19	21.77767065691867	29.154767730328107	31.853117825641323	17.2144437871119
20	22.33336011354811	29.10936309345565	33.09596324780706	15.461313545189178
21	21.44510908068392	28.17802026044976	34.41050660862664	15.966364050239685
22	20.440364113538372	24.869811637707517	34.47100554837359	20.218818700380524
23	18.373540173974014	28.06651718437691	35.13771116807993	18.422231473569145
24	18.234891698376877	28.411008129012465	35.07246482662246	18.2816353459882
25	18.69843287052253	30.306803878748926	32.14356142772628	18.851201823002256
26	18.2706798035793	31.267483219760877	31.462248418141403	18.999588558518422
27	17.99739988460162	30.476858242584925	33.52030792377864	18.005433949034817
28	16.242808903691046	30.069068608475696	34.406367848161054	19.28175463967221
29	17.745422409196813	27.955135836553048	33.843131240094365	20.456310514155778
30	18.80713619686866	28.658359930955733	34.0417917424425	18.4927121297331
31	19.326185450552767	29.615387424498053	32.022563548232384	19.035863576716793
32	20.069701595370432	30.01976866763562	31.568517179507783	18.342012557486164
33	19.308534854449533	29.003946429832183	32.64276897682537	19.044749738892904
34	18.215049993791858	28.967549683384824	33.217082855549954	19.60031746727336
35	18.01699813268866	29.301450270358444	32.30253852090439	20.379013076048505
36	18.962461442577133	30.134801862929123	31.358535950003773	19.54420074448997
37	18.417118887111656	31.569734461997655	31.753422389720292	18.259724261170394
38	18.434160841969952	30.940399414730578	31.05981482698764	19.56562491631183
39	19.26824280403456	29.43267332276732	31.240824733232543	20.058259139965575
40	19.92338424008706	28.471141884012457	32.746724901460986	18.8587489744395
41	18.327283439358638	28.821475784599425	31.105097735611114	21.74614304043082
42	18.484312880552938	29.097555453303826	31.838388707513797	20.57974295862944
43	18.508171617354552	28.91885838378969	31.672838288890347	20.900131709965404
44	18.57061820908531	29.523117411765277	31.07965653157266	20.826607847576756
45	18.13982193591738	29.14941168737264	30.913740928202245	21.797025448507735
46	18.79106806800227	30.162068990702394	30.641556563465457	20.405306377829877
47	17.123147600371027	29.359514645125635	32.89133806125852	20.62599969324481
48	17.74870907191948	29.315692475490017	32.198217411521824	20.737381041068677
49	18.55479353671689	28.722632446421308	32.43631786654202	20.28625615031978
50	17.458995839328452	28.585566438061015	32.26675041570197	21.688687306908566
51	17.60640874885271	28.375220023810044	30.896333788596987	23.122037438740257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	669.0
2	1049.0
3	5972.0
4	10895.0
5	9617.0
6	8339.0
7	6474.0
8	4609.0
9	4641.5
10	4674.0
11	4709.0
12	4744.0
13	4742.5
14	4741.0
15	4767.0
16	4793.0
17	4739.5
18	4686.0
19	4868.0
20	5050.0
21	5567.5
22	6085.0
23	6868.5
24	7652.0
25	9272.0
26	13307.5
27	15723.0
28	18641.5
29	21560.0
30	24296.0
31	27032.0
32	30700.0
33	34368.0
34	38315.5
35	42263.0
36	44684.5
37	47106.0
38	50368.0
39	53630.0
40	57130.0
41	60630.0
42	63920.5
43	67211.0
44	73378.5
45	79546.0
46	75924.5
47	72303.0
48	67759.5
49	63216.0
50	55299.5
51	47383.0
52	41425.5
53	35468.0
54	30409.5
55	25351.0
56	21842.0
57	18333.0
58	15570.5
59	12808.0
60	10699.0
61	8590.0
62	6721.5
63	4853.0
64	3613.0
65	2373.0
66	1849.5
67	1326.0
68	1060.5
69	795.0
70	643.0
71	491.0
72	378.5
73	266.0
74	215.5
75	135.0
76	105.0
77	77.5
78	50.0
79	38.5
80	27.0
81	22.5
82	18.0
83	13.0
84	8.0
85	5.0
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.02361837686442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.57120632191099	34.78060360038915
2	10.391257145898788	9.564865066774225
3	4.2721383419936005	5.898577941152512
4	2.2042749347451775	4.057948335775992
5	1.2744767556711758	2.9328015916597234
6	0.800301765487206	2.209966982466841
7	0.6116123393171322	1.9704029029519101
8	0.4875923063147325	1.795260978345954
9	0.42978096266513816	1.7802067510207562
>10	3.9187503247867697	31.453900790955664
>50	0.025739334240564026	0.7694069694321779
>100	0.010038184236026336	0.8407098201215216
>500	0.0015443360360654133	0.4131880868414419
>1k	0.0010295573573769422	0.8230592968389733
>5k	2.5738933934423556E-4	0.7091008852731857
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5986	0.728665298441148	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2464	0.2999384055060122	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	1662	0.20231234981777282	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	1530	0.1862442209513793	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	1292	0.15727289769227587	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.217282489878296E-4	0.0	0.0	0.12391935746961054	0.0
2	1.217282489878296E-4	0.0	0.0	0.3667672142003306	0.0
3	1.217282489878296E-4	0.0	0.0	0.4909300281679168	0.0
4	1.217282489878296E-4	0.0	0.0	0.7542282307285922	0.0
5	1.217282489878296E-4	0.0	0.0	1.418864470202142	0.0
6	1.217282489878296E-4	0.0	0.0	1.7514260464368925	0.0
7	2.434564979756592E-4	0.0	0.0	2.0749797322465433	0.0
8	2.434564979756592E-4	0.0	0.0	2.774795435677576	0.0
9	2.434564979756592E-4	0.0	0.0	3.0116786082078923	0.0
10	2.434564979756592E-4	0.0	0.0	3.7124681376308275	0.0
11	2.434564979756592E-4	0.0	0.0	4.395607070950527	0.0
12	2.434564979756592E-4	0.0	0.0	5.1583562791082676	0.0
13	2.434564979756592E-4	0.0	0.0	5.400717222843037	0.0
14	2.434564979756592E-4	0.0	0.0	5.521228189340988	0.0
15	2.434564979756592E-4	0.0	0.0	5.74094767876402	0.0
16	2.434564979756592E-4	0.0	0.0	6.114409946658681	0.0
17	2.434564979756592E-4	0.0	0.0	6.588906661213241	0.0
18	2.434564979756592E-4	0.0	0.0	7.105034436921638	0.0
19	2.434564979756592E-4	0.0	0.0	7.487626323490387	0.0
20	2.434564979756592E-4	0.0	0.0	7.867053275585452	0.0
21	2.434564979756592E-4	0.0	0.0	8.348245043834343	0.0
22	2.434564979756592E-4	0.0	0.0	8.93107989998807	0.0
23	2.434564979756592E-4	0.0	0.0	9.490421204087149	0.0
24	2.434564979756592E-4	0.0	0.0	9.911722673834026	0.0
25	2.434564979756592E-4	0.0	0.0	10.26315212866189	0.0
26	2.434564979756592E-4	0.0	0.0	10.59693098738652	0.0
27	2.434564979756592E-4	0.0	0.0	10.974045102750814	0.0
28	2.434564979756592E-4	0.0	0.0	11.365401423246688	0.0
29	2.434564979756592E-4	0.0	0.0	11.816648042244571	0.0
30	2.434564979756592E-4	0.0	0.0	12.33606248067564	0.0
31	2.434564979756592E-4	0.0	0.0	12.81506314044275	0.0
32	2.434564979756592E-4	0.0	0.0	13.270448519906221	0.0
33	2.434564979756592E-4	0.0	0.0	13.721329954157142	0.0
34	2.434564979756592E-4	0.0	0.0	14.195583212213725	0.0
35	2.434564979756592E-4	0.0	0.0	14.699781619521316	0.0
36	2.434564979756592E-4	0.0	0.0	15.222969633671008	0.0
37	2.434564979756592E-4	0.0	0.0	15.727411497476574	0.0
38	2.434564979756592E-4	0.0	0.0	16.28115330212221	0.0
39	2.434564979756592E-4	0.0	0.0	17.086263940927715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	20	7.0321094E-4	45.000004	9
CGACGGT	20	7.0321094E-4	45.000004	28
CCGTGCC	20	7.0321094E-4	45.000004	17
CATAGCG	20	7.0321094E-4	45.000004	1
GCGATCG	20	7.0321094E-4	45.000004	9
CCGCAAC	20	7.0321094E-4	45.000004	12
GTACGAG	20	7.0321094E-4	45.000004	1
CCGACGG	25	3.889831E-5	45.0	2
TATCGCG	35	1.211356E-7	45.0	1
CGGACGG	25	3.889831E-5	45.0	2
ACAGCGG	60	0.0	44.999996	2
CGCACGG	30	2.164743E-6	44.999996	2
TATCGAG	30	2.164743E-6	44.999996	1
CGGTTTT	920	0.0	43.532608	1
CGGTTTA	255	0.0	42.352936	1
TACGGGA	415	0.0	42.289154	4
AAGTGCG	70	0.0	41.785713	1
GACGGGT	65	0.0	41.538464	4
GCACGGG	185	0.0	41.351353	3
CGTTTTT	2885	0.0	41.100517	1
>>END_MODULE