FastQCFastQC Report
Sat 18 Jun 2016
SRR3549409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87548
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13461.5374423173573353No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.5608351989765614No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2790.318682322840042No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC2540.29012655914469776No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2380.2718508703796774No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG2350.2684241787362361No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2130.24329510668433316No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.19417919312834103No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC1660.18961027093708593TruSeq Adapter, Index 22 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1620.18504134874583084No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1350.15420112395485905No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.1484899712157902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.1130808242335633No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT970.11079636313793577No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA940.10736967149449445No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT920.10508521039886691No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCA206.9975737E-445.0000045
GGCACCG206.9975737E-445.0000048
TATACGG206.9975737E-445.0000042
TGACTAT206.9975737E-445.00000423
TGGTAAG206.9975737E-445.00000411
GGGTATA206.9975737E-445.0000047
ATGACGG206.9975737E-445.0000042
CTGGGCT206.9975737E-445.0000045
GGACCGA302.1425094E-645.0000048
GACGGGA302.1425094E-645.0000044
ATTGGGA206.9975737E-445.0000044
GGCGATG406.692062E-945.0000048
AGGGCAA206.9975737E-445.0000046
GCTACGA206.9975737E-445.00000410
ATTACGG206.9975737E-445.0000042
CGTGGGA206.9975737E-445.0000044
AAAAGTC206.9975737E-445.00000442
AGCGGTT206.9975737E-445.00000428
GTGGGAC206.9975737E-445.0000045
GACCGAT302.1425094E-645.0000049