Basic Statistics
Measure | Value |
---|---|
Filename | SRR3549409_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 87548 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1346 | 1.5374423173573353 | No Hit |
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 491 | 0.5608351989765614 | No Hit |
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 279 | 0.318682322840042 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC | 254 | 0.29012655914469776 | No Hit |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 238 | 0.2718508703796774 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG | 235 | 0.2684241787362361 | No Hit |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 213 | 0.24329510668433316 | No Hit |
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 170 | 0.19417919312834103 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC | 166 | 0.18961027093708593 | TruSeq Adapter, Index 22 (95% over 21bp) |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 162 | 0.18504134874583084 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC | 135 | 0.15420112395485905 | No Hit |
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 130 | 0.1484899712157902 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 99 | 0.1130808242335633 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 97 | 0.11079636313793577 | No Hit |
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 94 | 0.10736967149449445 | No Hit |
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT | 92 | 0.10508521039886691 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGGGCA | 20 | 6.9975737E-4 | 45.000004 | 5 |
GGCACCG | 20 | 6.9975737E-4 | 45.000004 | 8 |
TATACGG | 20 | 6.9975737E-4 | 45.000004 | 2 |
TGACTAT | 20 | 6.9975737E-4 | 45.000004 | 23 |
TGGTAAG | 20 | 6.9975737E-4 | 45.000004 | 11 |
GGGTATA | 20 | 6.9975737E-4 | 45.000004 | 7 |
ATGACGG | 20 | 6.9975737E-4 | 45.000004 | 2 |
CTGGGCT | 20 | 6.9975737E-4 | 45.000004 | 5 |
GGACCGA | 30 | 2.1425094E-6 | 45.000004 | 8 |
GACGGGA | 30 | 2.1425094E-6 | 45.000004 | 4 |
ATTGGGA | 20 | 6.9975737E-4 | 45.000004 | 4 |
GGCGATG | 40 | 6.692062E-9 | 45.000004 | 8 |
AGGGCAA | 20 | 6.9975737E-4 | 45.000004 | 6 |
GCTACGA | 20 | 6.9975737E-4 | 45.000004 | 10 |
ATTACGG | 20 | 6.9975737E-4 | 45.000004 | 2 |
CGTGGGA | 20 | 6.9975737E-4 | 45.000004 | 4 |
AAAAGTC | 20 | 6.9975737E-4 | 45.000004 | 42 |
AGCGGTT | 20 | 6.9975737E-4 | 45.000004 | 28 |
GTGGGAC | 20 | 6.9975737E-4 | 45.000004 | 5 |
GACCGAT | 30 | 2.1425094E-6 | 45.000004 | 9 |