##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87548
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.584319459039612	31.0	31.0	34.0	30.0	34.0
2	31.63866678850459	31.0	31.0	34.0	30.0	34.0
3	30.91960981404487	31.0	31.0	34.0	27.0	34.0
4	35.26570567003244	37.0	35.0	37.0	33.0	37.0
5	35.25485447982821	37.0	35.0	37.0	33.0	37.0
6	35.33666103166263	37.0	35.0	37.0	33.0	37.0
7	31.99779549504272	36.0	35.0	37.0	10.0	37.0
8	33.62991730250834	37.0	35.0	37.0	17.0	37.0
9	36.71652122264358	39.0	35.0	39.0	32.0	39.0
10	36.67305935029926	38.0	35.0	39.0	32.0	39.0
11	36.622321469365374	38.0	35.0	39.0	32.0	39.0
12	36.34269200895509	38.0	35.0	39.0	32.0	39.0
13	32.46436240690821	37.0	33.0	39.0	8.0	39.0
14	34.96448805226847	38.0	33.0	40.0	18.0	41.0
15	36.46221501347832	38.0	34.0	40.0	30.0	41.0
16	37.13711335495957	38.0	35.0	40.0	32.0	41.0
17	37.268721158678666	39.0	35.0	40.0	32.0	41.0
18	37.15502352994928	39.0	35.0	40.0	32.0	41.0
19	37.0954562068808	38.0	35.0	40.0	32.0	41.0
20	36.90859871156394	38.0	35.0	40.0	32.0	41.0
21	36.81965321880568	38.0	35.0	40.0	32.0	41.0
22	36.84062457166355	38.0	35.0	40.0	32.0	41.0
23	36.74769269429341	38.0	35.0	40.0	32.0	41.0
24	36.59666697126148	38.0	35.0	40.0	32.0	41.0
25	36.463642801663084	38.0	35.0	40.0	32.0	41.0
26	36.302245625257	38.0	35.0	40.0	31.0	41.0
27	36.25825832686069	38.0	35.0	40.0	31.0	41.0
28	36.191951843560105	38.0	35.0	40.0	31.0	41.0
29	36.258246904555214	38.0	35.0	40.0	31.0	41.0
30	36.08493626353543	38.0	35.0	40.0	31.0	41.0
31	35.874308950518575	38.0	35.0	40.0	30.0	41.0
32	35.56252570018732	37.0	34.0	40.0	30.0	41.0
33	35.22818339653676	37.0	34.0	40.0	27.0	41.0
34	34.919872527070865	37.0	34.0	40.0	25.0	41.0
35	34.64965504637456	37.0	34.0	40.0	23.0	41.0
36	34.357712340658836	37.0	34.0	40.0	22.0	41.0
37	34.15658838579979	37.0	33.0	40.0	21.0	41.0
38	34.05294238589117	37.0	33.0	40.0	21.0	41.0
39	33.88967195138667	37.0	33.0	40.0	19.0	41.0
40	33.762096221501345	37.0	33.0	40.0	18.0	41.0
41	33.73118746287751	37.0	33.0	40.0	18.0	41.0
42	33.66584045323708	37.0	33.0	40.0	18.0	41.0
43	33.606193174030246	37.0	33.0	40.0	18.0	41.0
44	33.3817677159958	36.0	33.0	40.0	17.0	41.0
45	33.382738611961436	36.0	33.0	40.0	18.0	41.0
46	33.27046877141682	36.0	33.0	40.0	17.0	41.0
47	33.144058116690275	36.0	32.0	40.0	17.0	41.0
48	33.0987458308585	36.0	32.0	40.0	17.0	41.0
49	33.0305660894595	36.0	32.0	40.0	15.0	41.0
50	32.79635171563028	36.0	32.0	40.0	15.0	41.0
51	31.065643989582856	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	3.0
13	1.0
14	0.0
15	1.0
16	9.0
17	31.0
18	41.0
19	100.0
20	160.0
21	263.0
22	506.0
23	742.0
24	1120.0
25	1548.0
26	1967.0
27	2053.0
28	2043.0
29	2053.0
30	2327.0
31	2865.0
32	3773.0
33	5537.0
34	8226.0
35	9780.0
36	8127.0
37	11098.0
38	12785.0
39	10381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.71234065883858	21.79261662173893	26.370676657376524	19.124366062045965
2	34.49993146616713	27.48092474985151	25.790423539087133	12.22872024489423
3	29.12002558596427	26.66651436926029	32.23488828985243	11.978571754923014
4	25.24101064558871	25.886370905103483	35.10988257869968	13.762735870608122
5	23.30036094485311	30.66089459496505	32.213185909443965	13.825558550737881
6	20.152373555078356	40.25677342714854	30.456435326906384	9.134417690866725
7	67.88161922602458	15.349294101521451	14.369260291497236	2.399826380956732
8	73.91830767122036	8.477635125873807	14.299584228080594	3.3044729748252384
9	68.22086169872527	8.96079864759903	17.059213231598665	5.759126422077032
10	38.391510942568644	27.19308265180244	22.929136016813633	11.486270388815278
11	34.80604925298122	22.517933019600676	29.799652761913464	12.876364965504639
12	32.2828619728606	23.251245031297117	30.83337140768493	13.632521588157353
13	17.321926257595834	40.24649335221821	29.170283730068075	13.261296660117877
14	14.068853657422215	36.943162607940785	33.38739891259652	15.600584822040481
15	14.259606158907115	25.91149997715539	46.30374194727464	13.525151916662859
16	15.751359254351899	24.704162288116233	42.88047699547677	16.6640014620551
17	16.642299081646637	24.74185589619409	33.712934618723445	24.90291040343583
18	18.27226207337689	27.608854571206653	37.381779138301276	16.73710421711518
19	24.259834605016675	26.744186046511626	32.50217023804085	16.49380911043085
20	25.550555124046237	25.752729931009277	33.655823091332756	15.040891853611733
21	18.610362315529766	30.820806871658974	34.56732306848814	16.001507744323114
22	17.854205692877052	26.741901585416	32.07040709096724	23.33348563073971
23	17.85991684561612	29.656873943436747	33.013889523461415	19.469319687485722
24	16.762233289167085	25.354091469822272	40.91812491433271	16.965550326677935
25	15.447525928633436	29.5951934938548	36.057933933385115	18.89934664412665
26	14.385251519166628	33.91853611732992	34.51478046328871	17.18143190021474
27	17.38132224608215	33.45479051491753	33.788321834879156	15.375565404121167
28	13.112806688902086	30.893909626719058	40.11171014757618	15.881573536802668
29	15.502352994928497	26.697354594051266	38.70905103486088	19.091241376159367
30	17.240827888701055	31.937908347420844	34.0578882441632	16.7633755197149
31	23.343765705670034	29.532370813725038	31.06067528669987	16.06318819390506
32	23.107323982272582	28.458674098780097	32.55813953488372	15.875862384063598
33	22.536208708365695	30.114908393110067	29.42271668113492	17.926166217389316
34	17.168867364188788	28.985242381322244	33.93566957554713	19.910220678941837
35	20.12039109973957	27.001187919769727	32.531868232284005	20.346552748206697
36	23.366610316626307	29.144012427468358	30.803673413441768	16.68570384246356
37	18.4470233471924	33.80659752364417	31.707177776762464	16.03920135240097
38	18.822817197423127	33.5678713391511	27.39754191986111	20.21176954356467
39	20.23232969342532	30.512404623749255	30.74998857769452	18.505277105130897
40	22.67099191300772	27.292456709462236	31.69118654909307	18.34536482843697
41	17.104902453511215	27.630556951615116	31.45817151733906	23.806369077534608
42	20.102115410974548	27.349568236852928	30.69401928085165	21.854297071320875
43	20.497327180518116	26.681363366381873	29.98812080230274	22.833188650797275
44	17.600630511262395	31.747155845935943	30.698588203042902	19.95362543975876
45	16.967834787773565	33.4776351258738	28.98866907296569	20.56586101338694
46	19.355096632704345	33.03901859551332	29.08347420843423	18.522410563348107
47	18.826243889066568	27.27418102069722	33.3359985379449	20.563576552291316
48	19.57440489788459	26.60369168913053	34.21323159866588	19.608671814319003
49	18.190021473934298	26.18449307808288	35.012792982135515	20.612692465847307
50	16.54863617672591	31.106364508612415	32.515877004614616	19.829122310047058
51	17.82108100699045	29.41700552839585	30.13661077351853	22.62530269109517
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	161.0
2	195.0
3	1293.0
4	2391.0
5	2039.5
6	1688.0
7	1342.5
8	997.0
9	958.0
10	919.0
11	898.0
12	877.0
13	865.5
14	854.0
15	787.0
16	720.0
17	721.5
18	723.0
19	726.0
20	729.0
21	663.0
22	597.0
23	621.5
24	646.0
25	933.0
26	1303.0
27	1386.0
28	1703.5
29	2021.0
30	2147.5
31	2274.0
32	2411.5
33	2549.0
34	2809.0
35	3069.0
36	3358.0
37	3647.0
38	4204.0
39	4761.0
40	5330.0
41	5899.0
42	6588.5
43	7278.0
44	7166.0
45	7054.0
46	7004.5
47	6955.0
48	7390.0
49	7825.0
50	7103.5
51	6382.0
52	5650.0
53	4918.0
54	4055.5
55	3193.0
56	2617.5
57	2042.0
58	1693.0
59	1344.0
60	1145.0
61	946.0
62	732.5
63	519.0
64	423.5
65	328.0
66	256.5
67	185.0
68	165.0
69	145.0
70	102.5
71	60.0
72	48.0
73	36.0
74	32.0
75	18.5
76	9.0
77	7.0
78	5.0
79	3.5
80	2.0
81	1.5
82	1.0
83	2.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	87548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.24896057020149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41907151819323	37.836386896331156
2	8.529627612982647	8.230913327546032
3	3.3877038895859477	4.903595741764518
4	1.9341398167657016	3.732809430255403
5	1.2428683033072132	2.998355188011148
6	0.9256409649392771	2.6796728651711064
7	0.7315167728036741	2.470644674921186
8	0.620250467555219	2.394115228217663
9	0.49241258492933404	2.1382555855073786
>10	3.6481143912312683	26.834422259788916
>50	0.040245259345185955	1.4186503403847033
>100	0.026041050164532092	2.824736144743455
>500	0.0	0.0
>1k	0.0023673681967756443	1.5374423173573353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1346	1.5374423173573353	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	491	0.5608351989765614	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	279	0.318682322840042	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	254	0.29012655914469776	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	238	0.2718508703796774	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	235	0.2684241787362361	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	213	0.24329510668433316	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	170	0.19417919312834103	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	166	0.18961027093708593	TruSeq Adapter, Index 22 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	162	0.18504134874583084	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	135	0.15420112395485905	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	130	0.1484899712157902	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99	0.1130808242335633	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	97	0.11079636313793577	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	94	0.10736967149449445	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	92	0.10508521039886691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14506327957234888	0.0
2	0.0	0.0	0.0	0.5002969799424316	0.0
3	0.0	0.0	0.0	0.6544981038972907	0.0
4	0.0	0.0	0.0	0.9777493489285878	0.0
5	0.0	0.0	0.0	1.8686891762233289	0.0
6	0.0	0.0	0.0	2.226207337689039	0.0
7	0.0	0.0	0.0	2.5974322657285147	0.0
8	0.0	0.0	0.0	3.361584502215927	0.0
9	0.0	0.0	0.0	3.5751816146571023	0.0
10	0.0	0.0	0.0	4.487823822360305	0.0
11	0.0	0.0	0.0	5.190295609265775	0.0
12	0.0	0.0	0.0	6.154338191620597	0.0
13	0.0	0.0	0.0	6.422762370356833	0.0
14	0.0	0.0	0.0	6.534700964042583	0.0
15	0.0	0.0	0.0	6.779138301274729	0.0
16	0.0	0.0	0.0	7.214328139991776	0.0
17	0.0	0.0	0.0	7.648375748161008	0.0
18	0.0011422305478137706	0.0	0.0	8.265180243980446	0.0
19	0.0011422305478137706	0.0	0.0	8.553022342029514	0.0
20	0.0011422305478137706	0.0	0.0	8.89340704527802	0.0
21	0.0011422305478137706	0.0	0.0	9.30575227303879	0.0
22	0.0011422305478137706	0.0	0.0	9.719239731347376	0.0
23	0.0011422305478137706	0.0	0.0	10.116735961986567	0.0
24	0.0011422305478137706	0.0	0.0	10.437702745922238	0.0
25	0.0011422305478137706	0.0	0.0	10.727829305066935	0.0
26	0.0011422305478137706	0.0	0.0	11.017955864211633	0.0
27	0.0011422305478137706	0.0	0.0	11.268104354182848	0.0
28	0.0011422305478137706	0.0	0.0	11.528532919084387	0.0
29	0.0011422305478137706	0.0	0.0	11.818659478229085	0.0
30	0.0011422305478137706	0.0	0.0	12.221866861607348	0.0
31	0.0011422305478137706	0.0	0.0	12.617078631150912	0.0
32	0.0011422305478137706	0.0	0.0	13.007721478503221	0.0
33	0.0011422305478137706	0.0	0.0	13.373235253803628	0.0
34	0.0011422305478137706	0.0	0.0	13.693059807191483	0.0
35	0.0011422305478137706	0.0	0.0	14.112258418239138	0.0
36	0.0011422305478137706	0.0	0.0	14.577146251199341	0.0
37	0.0011422305478137706	0.0	0.0	15.004340476081692	0.0
38	0.0011422305478137706	0.0	0.0	15.356147484808334	0.0
39	0.0011422305478137706	0.0	0.0	15.741079179421574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGCA	20	6.9975737E-4	45.000004	5
GGCACCG	20	6.9975737E-4	45.000004	8
TATACGG	20	6.9975737E-4	45.000004	2
TGACTAT	20	6.9975737E-4	45.000004	23
TGGTAAG	20	6.9975737E-4	45.000004	11
GGGTATA	20	6.9975737E-4	45.000004	7
ATGACGG	20	6.9975737E-4	45.000004	2
CTGGGCT	20	6.9975737E-4	45.000004	5
GGACCGA	30	2.1425094E-6	45.000004	8
GACGGGA	30	2.1425094E-6	45.000004	4
ATTGGGA	20	6.9975737E-4	45.000004	4
GGCGATG	40	6.692062E-9	45.000004	8
AGGGCAA	20	6.9975737E-4	45.000004	6
GCTACGA	20	6.9975737E-4	45.000004	10
ATTACGG	20	6.9975737E-4	45.000004	2
CGTGGGA	20	6.9975737E-4	45.000004	4
AAAAGTC	20	6.9975737E-4	45.000004	42
AGCGGTT	20	6.9975737E-4	45.000004	28
GTGGGAC	20	6.9975737E-4	45.000004	5
GACCGAT	30	2.1425094E-6	45.000004	9
>>END_MODULE