FastQCFastQC Report
Sat 18 Jun 2016
SRR3549405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273268
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32351.183819547111261No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC14600.5342740459914809No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG13020.47645534786363564No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.44791193992710454No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC9060.3315426614166313No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6010.2199306175622466No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4170.15259745012222434No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4150.1518655678674415No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC3910.14308298081004728No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.13832574615395876No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3660.13393445262526166No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3590.13137286473352167No Hit
GAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT3510.12844533571439026No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.1277134534596074No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT3240.11856492527482179No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT3090.11307580836395041No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGG650.045.0000042
CGGGAAT502.1827873E-1145.0000046
AAATCGG207.023839E-445.02
CAACGAG207.023839E-445.014
CGGTTTA1350.045.01
CTACGGG207.023839E-445.03
TCAGACG207.023839E-445.022
GCGGGAC406.7830115E-945.05
CAAATCG207.023839E-445.01
CGAATGG207.023839E-445.02
CGGGACA406.7830115E-945.06
GGGCACA551.8189894E-1245.07
GCTACGA453.8198777E-1045.010
GGATTAC207.023839E-445.08
CACAACG207.023839E-445.012
AGCTACG453.8198777E-1045.09
AGGGCCC207.023839E-445.06
AGGGACG453.8198777E-1045.06
TAGACGG302.1594096E-644.9999962
TTAACGG302.1594096E-644.9999962
Produced by FastQC (version 0.11.5)