FastQCFastQC Report
Sat 18 Jun 2016
SRR3549404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320124
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.9040246904324574No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.3573615224100661No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7040.21991478302157913No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG6940.21679099349002262No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC6790.21210530919268783No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6140.19180067723757044No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5730.17899314015818868No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC5540.1730579400482313No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4580.14306956054528872No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4480.13994577101373218No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4300.13432294985693044No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.11339355999550176No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC3570.11151928627656782No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA3210.10027364396296436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT302.1605774E-645.0000046
GGCCGAT302.1605774E-645.0000048
ACGGGAT650.045.0000045
GAGGGCC302.1605774E-645.0000045
ATATGCG207.0256524E-445.01
ACGGGTA253.8844788E-545.05
GCATTCG207.0256524E-445.029
CCCCGCT207.0256524E-445.034
CCTCCGC253.8844788E-545.045
GCGGGTA351.2082637E-745.05
CGGGATC406.7884685E-945.06
CGGGAAC207.0256524E-445.06
AATTCGT253.8844788E-545.031
TCGTACA253.8844788E-545.034
TCGTAAG207.0256524E-445.01
GCGGCTA750.045.024
TTACGTG207.0256524E-445.023
ACGTAGG207.0256524E-445.02
CGGTAGA207.0256524E-445.019
AGGGCCG207.0256524E-445.06