##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549404_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320124
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.554685059539427	31.0	31.0	34.0	30.0	34.0
2	31.674576101760568	31.0	31.0	34.0	30.0	34.0
3	31.243049568292285	31.0	31.0	34.0	28.0	34.0
4	35.35557471479802	37.0	35.0	37.0	33.0	37.0
5	35.23957903812273	37.0	35.0	37.0	33.0	37.0
6	35.26739013632218	37.0	35.0	37.0	32.0	37.0
7	32.0925953692944	36.0	35.0	37.0	11.0	37.0
8	33.638886806362535	37.0	35.0	37.0	19.0	37.0
9	36.6071865901963	39.0	35.0	39.0	32.0	39.0
10	36.467999900038734	38.0	35.0	39.0	32.0	39.0
11	36.440507428371504	38.0	35.0	39.0	32.0	39.0
12	36.44838250178056	38.0	35.0	39.0	32.0	39.0
13	32.72592495408029	37.0	34.0	39.0	8.0	39.0
14	35.25053104422037	39.0	34.0	40.0	18.0	41.0
15	36.62758181204783	39.0	35.0	40.0	30.0	41.0
16	37.2464263847759	39.0	36.0	40.0	32.0	41.0
17	37.392519773587736	39.0	36.0	40.0	32.0	41.0
18	37.30681860778948	39.0	36.0	40.0	32.0	41.0
19	37.32542702202896	39.0	36.0	40.0	32.0	41.0
20	37.21491359598156	39.0	35.0	40.0	32.0	41.0
21	37.160059851807425	39.0	35.0	40.0	32.0	41.0
22	37.137980907398386	39.0	35.0	40.0	32.0	41.0
23	37.06034536617061	39.0	35.0	40.0	32.0	41.0
24	36.946967425122764	39.0	35.0	40.0	32.0	41.0
25	36.77468730866789	38.0	35.0	40.0	31.0	41.0
26	36.627269433094675	38.0	35.0	40.0	31.0	41.0
27	36.62279929027502	38.0	35.0	40.0	31.0	41.0
28	36.53505828991266	38.0	35.0	40.0	31.0	41.0
29	36.50092464170134	38.0	35.0	40.0	31.0	41.0
30	36.38698754232735	38.0	35.0	40.0	30.0	41.0
31	36.25912458922168	38.0	35.0	40.0	30.0	41.0
32	36.06941997475978	38.0	35.0	40.0	30.0	41.0
33	35.86800739713361	38.0	35.0	40.0	29.0	41.0
34	35.631761442441054	38.0	35.0	40.0	28.0	41.0
35	35.5113143656833	38.0	35.0	40.0	27.0	41.0
36	35.329025627569315	38.0	34.0	40.0	26.0	41.0
37	35.24740413089928	38.0	34.0	40.0	25.0	41.0
38	35.15726718396621	38.0	34.0	40.0	25.0	41.0
39	35.019736102260374	38.0	34.0	40.0	24.0	41.0
40	34.94519623645837	38.0	34.0	40.0	24.0	41.0
41	34.88703752295985	38.0	34.0	40.0	24.0	41.0
42	34.903018830203294	38.0	34.0	40.0	24.0	41.0
43	34.85538416363659	38.0	34.0	40.0	24.0	41.0
44	34.6605128012895	38.0	34.0	40.0	23.0	41.0
45	34.62731941372718	38.0	34.0	40.0	23.0	41.0
46	34.53625157751372	38.0	34.0	40.0	23.0	41.0
47	34.36637990278767	38.0	33.0	40.0	23.0	41.0
48	34.30617510714598	38.0	33.0	40.0	23.0	41.0
49	34.20643875498244	37.0	33.0	40.0	22.0	41.0
50	34.030847421624124	37.0	33.0	40.0	22.0	41.0
51	32.148376878959404	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	13.0
10	13.0
11	8.0
12	5.0
13	4.0
14	9.0
15	13.0
16	32.0
17	67.0
18	123.0
19	289.0
20	500.0
21	800.0
22	1281.0
23	1883.0
24	2921.0
25	4106.0
26	5181.0
27	5840.0
28	6079.0
29	6727.0
30	7984.0
31	10272.0
32	13277.0
33	18504.0
34	25948.0
35	31074.0
36	33700.0
37	46784.0
38	52962.0
39	43716.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.44633954342692	23.250990241281503	26.39383488898052	15.908835326311054
2	32.939423473404055	27.35596206469993	26.59688120853169	13.107733253364321
3	30.973622721195536	25.9643138283915	29.801576888955527	13.260486561457435
4	26.773375317064634	26.475053416800993	31.908260549037248	14.843310717097125
5	23.744548987267432	32.13473529007509	29.266784121153055	14.853931601504417
6	22.758056253201882	40.89821444190376	27.191025977433746	9.15270332746061
7	71.91557021654108	14.93202633979333	10.473441541402707	2.6789619022628735
8	77.39126088640653	7.427746748135097	11.594881983231497	3.586110382226887
9	70.2990091339606	9.92865264709925	13.8277667403881	5.944571478552061
10	35.21041846284565	31.005485374417415	21.28706376279192	12.497032399945022
11	29.30302007971911	26.1495545476128	29.214929214929214	15.332496157738875
12	30.277954792517896	22.992340468068623	30.809936149741972	15.919768589671502
13	19.854806262573252	35.112019092601614	28.59766840349365	16.435506241331485
14	16.38052754557609	32.809473828891306	31.087953418050507	19.7220452074821
15	16.781622121427947	28.257800102460294	39.12171533530756	15.838862440804188
16	18.223250990241283	26.60718971398583	37.04814384426035	18.121415451512537
17	18.090489935150128	26.821794054803767	32.56519348752359	22.52252252252252
18	18.667141482675465	28.59423223500893	33.92372955479752	18.81489672751809
19	24.014132023840762	27.318788969274404	30.753707938173957	17.913371068710873
20	24.879734103035073	27.042333595731655	32.20751958616036	15.87041271507291
21	21.563206757381515	29.06030163311717	31.702715197860833	17.67377641164049
22	18.91173420299634	27.780172683085304	31.043283227749246	22.26480988616911
23	19.39717109620022	28.906923567117744	32.397758368632154	19.29814696804988
24	18.427546825605077	27.053579238045256	35.75989304144644	18.758980894903225
25	17.673464032687335	30.059601904262102	32.58487336157239	19.68206070147818
26	17.438242680961128	32.15816371156177	31.087953418050507	19.315640189426595
27	18.910484687183715	31.860466569204434	30.493496270195298	18.73555247341655
28	16.102822656220713	30.65780759955517	34.514438155214854	18.72493158900926
29	17.84902100436081	28.423048568679636	33.4807761992228	20.24715422773675
30	17.67939923279729	30.596581324736665	31.99572665592083	19.72829278654521
31	21.560395346803112	30.07615798877935	29.298334395421776	19.065112268995765
32	21.09057740125701	30.73121665354675	29.977133860629007	18.20107208456723
33	21.13587234946458	29.476390398720497	29.031875148380003	20.35586210343492
34	18.48408741612625	29.327073259112098	31.79924029438593	20.38959903037573
35	20.06972298234434	26.780560032987218	31.279129337381768	21.870587647286676
36	20.555784633454536	31.121690344991315	29.47732753557996	18.845197485974186
37	18.696192725318937	31.62868138596294	31.393460034236732	18.28166585448139
38	18.759918031762695	31.22914870487686	28.981269757968786	21.02966350539166
39	20.333058439854558	29.050930264522496	31.572765553348077	19.04324574227487
40	20.718846447001788	28.135659931776434	31.809236420886904	19.33625720033487
41	17.112431432819783	27.715197860828926	32.54020317127113	22.632167535080157
42	18.12984968324774	28.304032187527334	30.853981582136925	22.712136547088
43	19.841061588634403	27.275368294785768	30.885219477452488	21.998350639127338
44	18.747110494683312	29.830003373692694	29.835626194849496	21.5872599367745
45	17.90524921592883	30.591270882533017	28.98876685284452	22.514713048693633
46	18.80646249578288	32.03914733040947	28.382751683722557	20.771638490085092
47	18.073621471679722	28.058189951393835	32.809473828891306	21.058714748035136
48	18.74211243143282	27.22882383076558	32.9391110944509	21.089952643350703
49	18.71962114680561	27.856393147655282	32.83852507153478	20.585460634004324
50	17.491659481950744	28.439604653196888	32.19783583861254	21.87090002623983
51	17.898376878959404	27.428121602878885	30.20673239119841	24.466769126963303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	522.5
2	801.0
3	3143.0
4	5485.0
5	4788.5
6	4092.0
7	3236.0
8	2380.0
9	2284.0
10	2188.0
11	2041.0
12	1894.0
13	1901.0
14	1908.0
15	1869.5
16	1831.0
17	1739.0
18	1647.0
19	1718.0
20	1789.0
21	1862.5
22	1936.0
23	2241.0
24	2546.0
25	3167.0
26	4470.0
27	5152.0
28	6110.5
29	7069.0
30	8140.0
31	9211.0
32	10019.5
33	10828.0
34	11939.0
35	13050.0
36	13489.0
37	13928.0
38	15630.0
39	17332.0
40	19267.0
41	21202.0
42	22647.5
43	24093.0
44	25039.5
45	25986.0
46	28242.5
47	30499.0
48	29010.0
49	27521.0
50	25747.5
51	23974.0
52	20751.0
53	17528.0
54	15033.0
55	12538.0
56	10740.5
57	8943.0
58	7860.5
59	6778.0
60	5972.5
61	5167.0
62	4077.5
63	2988.0
64	2265.0
65	1542.0
66	1179.0
67	816.0
68	683.5
69	551.0
70	435.0
71	319.0
72	274.0
73	229.0
74	200.5
75	128.0
76	84.0
77	64.5
78	45.0
79	37.0
80	29.0
81	20.5
82	12.0
83	8.0
84	4.0
85	3.5
86	3.0
87	1.5
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	320124.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.70193534880112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8394513216861	35.5741357188985
2	9.197119320531007	8.406523051642354
3	3.6082119739831082	4.947068110792382
4	1.959121557657378	3.5814258707400013
5	1.2223034375988324	2.7930816340879603
6	0.8367327929894456	2.294418480565526
7	0.5818949745665509	1.861560855520295
8	0.4628039153342868	1.6920827694223681
9	0.41048696802615803	1.688404398683118
>10	3.81212003940072	31.307362844360455
>50	0.04334210375236909	1.3169344828590657
>100	0.02099383150505378	2.10554765849359
>500	0.004063322226784603	1.1816817521870537
>1k	0.001354440742261534	1.2497723717473344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2894	0.9040246904324574	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1144	0.3573615224100661	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	704	0.21991478302157913	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	694	0.21679099349002262	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	679	0.21210530919268783	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	614	0.19180067723757044	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	573	0.17899314015818868	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	554	0.1730579400482313	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	458	0.14306956054528872	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	448	0.13994577101373218	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	430	0.13432294985693044	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	363	0.11339355999550176	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	357	0.11151928627656782	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	321	0.10027364396296436	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13932101310742087	0.0
2	0.0	0.0	0.0	0.4948082617985531	0.0
3	0.0	0.0	0.0	0.6391273381564644	0.0
4	0.0	0.0	0.0	0.9433844385300696	0.0
5	0.0	0.0	0.0	1.7596306431257887	0.0
6	0.0	0.0	0.0	2.1604128400244904	0.0
7	0.0	0.0	0.0	2.5368294785770513	0.0
8	0.0	0.0	0.0	3.314028314028314	0.0
9	0.0	0.0	0.0	3.5911084454773774	0.0
10	0.0	0.0	0.0	4.424223113543502	0.0
11	0.0	0.0	0.0	5.288263297972036	0.0
12	0.0	0.0	0.0	6.236645799752596	0.0
13	0.0	0.0	0.0	6.574952206020167	0.0
14	0.0	0.0	0.0	6.741137809098974	0.0
15	0.0	0.0	0.0	7.034149267158976	0.0
16	0.0	0.0	0.0	7.596743761792306	0.0
17	0.0	0.0	0.0	8.145905961439942	0.0
18	0.0	0.0	0.0	8.864065174744786	0.0
19	0.0	0.0	0.0	9.262973097924554	0.0
20	0.0	0.0	0.0	9.738101485674301	0.0
21	0.0	0.0	0.0	10.293823643338207	0.0
22	0.0	0.0	0.0	10.924829128712624	0.0
23	0.0	0.0	0.0	11.519911034474141	0.0
24	0.0	0.0	0.0	11.987854706301308	0.0
25	0.0	0.0	0.0	12.41737576689033	0.0
26	0.0	0.0	0.0	12.794104784396046	0.0
27	0.0	0.0	0.0	13.16958428608914	0.0
28	0.0	0.0	0.0	13.577863577863578	0.0
29	0.0	0.0	0.0	14.045807249690744	0.0
30	0.0	0.0	0.0	14.542177406255076	0.0
31	0.0	0.0	0.0	15.057915057915057	0.0
32	0.0	0.0	0.0	15.584273593982331	0.0
33	0.0	0.0	0.0	16.05190488685634	0.0
34	0.0	0.0	0.0	16.531406579950268	0.0
35	0.0	0.0	0.0	17.04495757893816	0.0
36	0.0	0.0	0.0	17.62410815808874	0.0
37	0.0	0.0	0.0	18.18514075795629	0.0
38	0.0	0.0	0.0	18.684322325099025	0.0
39	0.0	0.0	0.0	19.176943934225488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1605774E-6	45.000004	6
GGCCGAT	30	2.1605774E-6	45.000004	8
ACGGGAT	65	0.0	45.000004	5
GAGGGCC	30	2.1605774E-6	45.000004	5
ATATGCG	20	7.0256524E-4	45.0	1
ACGGGTA	25	3.8844788E-5	45.0	5
GCATTCG	20	7.0256524E-4	45.0	29
CCCCGCT	20	7.0256524E-4	45.0	34
CCTCCGC	25	3.8844788E-5	45.0	45
GCGGGTA	35	1.2082637E-7	45.0	5
CGGGATC	40	6.7884685E-9	45.0	6
CGGGAAC	20	7.0256524E-4	45.0	6
AATTCGT	25	3.8844788E-5	45.0	31
TCGTACA	25	3.8844788E-5	45.0	34
TCGTAAG	20	7.0256524E-4	45.0	1
GCGGCTA	75	0.0	45.0	24
TTACGTG	20	7.0256524E-4	45.0	23
ACGTAGG	20	7.0256524E-4	45.0	2
CGGTAGA	20	7.0256524E-4	45.0	19
AGGGCCG	20	7.0256524E-4	45.0	6
>>END_MODULE