##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	210907
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375511481363823	31.0	31.0	34.0	30.0	34.0
2	31.45555149900193	31.0	31.0	34.0	30.0	34.0
3	30.919305665530306	31.0	31.0	34.0	27.0	34.0
4	35.14587472203388	37.0	35.0	37.0	32.0	37.0
5	35.07935725224862	35.0	35.0	37.0	32.0	37.0
6	35.10715623473853	36.0	35.0	37.0	32.0	37.0
7	31.955430592630876	35.0	35.0	37.0	11.0	37.0
8	33.462317514354666	36.0	35.0	37.0	18.0	37.0
9	36.513411124334425	39.0	35.0	39.0	32.0	39.0
10	36.3793899680902	38.0	35.0	39.0	32.0	39.0
11	36.333862792605274	38.0	35.0	39.0	32.0	39.0
12	36.26158923127255	38.0	35.0	39.0	32.0	39.0
13	32.57381215417222	37.0	33.0	39.0	11.0	39.0
14	34.995410299326245	38.0	33.0	40.0	18.0	41.0
15	36.32208983106298	38.0	34.0	40.0	30.0	41.0
16	36.98066920491022	38.0	35.0	40.0	32.0	41.0
17	37.101276866106865	39.0	35.0	40.0	32.0	41.0
18	36.996775830105214	39.0	36.0	40.0	32.0	41.0
19	36.95716121323616	38.0	35.0	40.0	32.0	41.0
20	36.8615930244136	38.0	35.0	40.0	32.0	41.0
21	36.790357835444055	38.0	35.0	40.0	32.0	41.0
22	36.86208139132414	38.0	35.0	40.0	32.0	41.0
23	36.76277221713836	38.0	35.0	40.0	32.0	41.0
24	36.60903621027278	38.0	35.0	40.0	31.0	41.0
25	36.437576751838485	38.0	35.0	40.0	31.0	41.0
26	36.28241357565183	38.0	35.0	40.0	31.0	41.0
27	36.25310207816715	38.0	35.0	40.0	31.0	41.0
28	36.21458747220339	38.0	35.0	40.0	31.0	41.0
29	36.15443299653402	38.0	35.0	40.0	30.0	41.0
30	35.914000009482855	38.0	35.0	40.0	30.0	41.0
31	35.769647285296365	38.0	34.0	40.0	30.0	41.0
32	35.53669626897163	38.0	34.0	40.0	29.0	41.0
33	35.25463355886718	38.0	34.0	40.0	27.0	41.0
34	34.923089323730366	38.0	34.0	40.0	25.0	41.0
35	34.72846799774308	38.0	34.0	40.0	24.0	41.0
36	34.47066242467059	38.0	33.0	40.0	23.0	41.0
37	34.29806028249418	37.0	33.0	40.0	23.0	41.0
38	34.32793600971044	37.0	33.0	40.0	23.0	41.0
39	34.080869767243385	37.0	33.0	40.0	21.0	41.0
40	34.00834491031592	37.0	33.0	40.0	21.0	41.0
41	33.793719506702004	37.0	33.0	40.0	20.0	41.0
42	33.95573878534141	37.0	33.0	40.0	21.0	41.0
43	33.971617822073235	37.0	33.0	40.0	21.0	41.0
44	33.85449510921875	37.0	33.0	40.0	20.0	41.0
45	33.854950286145076	37.0	33.0	40.0	20.0	41.0
46	33.74040216778011	37.0	33.0	40.0	20.0	41.0
47	33.496024314034145	37.0	32.0	40.0	20.0	41.0
48	33.405135912985344	37.0	32.0	40.0	20.0	41.0
49	33.346114638205464	36.0	32.0	40.0	19.0	41.0
50	33.126093491444095	36.0	32.0	40.0	18.0	41.0
51	31.171701271176396	35.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	10.0
11	4.0
12	4.0
13	4.0
14	3.0
15	16.0
16	19.0
17	53.0
18	97.0
19	241.0
20	387.0
21	649.0
22	974.0
23	1488.0
24	2346.0
25	3361.0
26	4283.0
27	4677.0
28	4770.0
29	5290.0
30	6385.0
31	7815.0
32	10209.0
33	13601.0
34	18668.0
35	22140.0
36	21537.0
37	28297.0
38	30079.0
39	23493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.466883507896846	22.792036300359875	24.945117990393868	18.795962201349408
2	30.367413125216324	30.430474095217324	26.350476750416057	12.85163602915029
3	28.52679142939779	28.939769661509573	30.653795274694534	11.8796436343981
4	24.250499035119745	24.680072259337056	37.62938167059415	13.440047034949053
5	24.44963894038605	28.587481686240857	31.309060391546982	15.653818981826111
6	19.8518778418924	39.859274466945145	30.06159112784308	10.227256563319377
7	67.51601416738183	14.01755276022133	15.394937104979919	3.071495967416918
8	72.10429241324375	8.309349618552254	14.659067740757775	4.92729022744622
9	67.10967393211226	7.125889610112514	16.4650770244705	9.299359433304726
10	36.56445732005102	25.86637712356631	23.89868520248261	13.67048035390006
11	29.803657536260058	23.222557809840357	30.51202662785019	16.4617580260494
12	28.80084587045475	20.39761601084838	33.40240011000109	17.399138008695775
13	17.715391143963927	35.373411029505895	32.346958612089686	14.564239214440489
14	15.52911946971888	32.1473445641918	33.08330211894342	19.2402338471459
15	13.367977354947916	25.504132153034277	46.000369831252634	15.127520660765171
16	15.314807000241812	25.474735309875918	38.9731018885101	20.23735580137217
17	15.153598505502424	25.16986159776584	35.150563992660274	24.525975904071462
18	16.25029041236185	26.70418715357954	37.527915147434655	19.51760728662396
19	19.713902336100748	27.706050534121673	32.403381585248475	20.176665544529104
20	21.29421972717833	26.364226886732066	35.69772458951101	16.643828796578585
21	17.723925711332484	29.99189216099987	35.037718046342704	17.246464081324945
22	17.08193658816445	24.941798991972767	33.19757049315575	24.77869392670703
23	16.734390039211597	29.129426714144145	33.93296571474631	20.203217531897945
24	17.897461914493118	24.821840906181396	37.10355749216479	20.177139687160693
25	15.945890842883356	30.3787925483744	33.10700925052274	20.5683073582195
26	15.292048153925663	30.246980896793374	32.458382130512504	22.002588818768462
27	17.976169591336465	31.879453977345467	30.614915578904444	19.529460852413624
28	13.867723688640016	28.141787612549606	36.36958469846899	21.620904000341383
29	17.296249057641518	25.330121807242058	33.468305935791605	23.905323199324823
30	17.447026414486004	30.97478983627855	32.344587898931756	19.23359585030369
31	20.89025020506669	27.31867600411556	29.95443489310454	21.836638897713208
32	24.812832196181255	26.800438107791585	29.2778333578307	19.10889633819646
33	21.672585547184305	27.991010255705124	29.311023342041754	21.025380855068825
34	18.866135310824202	25.132878472502096	32.76088512946465	23.24010108720905
35	21.5469377498139	25.803790296196905	30.89892701522472	21.750344938764478
36	21.116890382965003	26.508366246734344	31.525743574181988	20.84899979611867
37	18.978981257141772	28.896148539403622	33.68735983158454	18.437510371870065
38	20.39809015347997	27.786180638859783	29.24179851783013	22.573930689830117
39	22.83470913720265	25.828919855670986	33.166277079471044	18.170093927655316
40	23.70618329405852	24.52787247459781	32.74523842262229	19.02070580872138
41	17.637157609752165	25.922800096725098	34.14870061211814	22.2913416814046
42	20.538436372429555	24.809039055128565	32.516227531566045	22.136297040875835
43	20.87792249664544	24.902445153551092	32.59920249209367	21.620429857709798
44	18.76040150398043	26.779101689370194	30.86004731943463	23.600449487214746
45	18.040178846600636	28.672827359926412	29.339946042568528	23.947047750904428
46	21.917717287714492	28.787095734138745	29.790855685207223	19.504331292939543
47	17.463621406591532	25.692840920405676	35.80013939793369	21.043398275069105
48	19.563599121887844	25.493701015139376	32.823471956834055	22.119227906138725
49	19.69493663083729	23.551612796161344	35.933373477409475	20.820077095591895
50	18.870876737140065	24.730331378285218	33.602962443162156	22.795829441412565
51	18.322767855026147	24.713262243548105	29.588396781519815	27.375573119905933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161.0
1	251.5
2	342.0
3	2354.0
4	4366.0
5	3843.0
6	3320.0
7	2601.0
8	1882.0
9	1801.0
10	1720.0
11	1633.0
12	1546.0
13	1533.5
14	1521.0
15	1409.0
16	1297.0
17	1256.0
18	1215.0
19	1196.5
20	1178.0
21	1256.0
22	1334.0
23	1377.0
24	1420.0
25	1879.0
26	2650.5
27	2963.0
28	3498.5
29	4034.0
30	4619.0
31	5204.0
32	5790.0
33	6376.0
34	6527.0
35	6678.0
36	7499.5
37	8321.0
38	8675.5
39	9030.0
40	10495.5
41	11961.0
42	13521.0
43	15081.0
44	15172.5
45	15264.0
46	16086.5
47	16909.0
48	21972.5
49	27036.0
50	23242.5
51	19449.0
52	17271.5
53	15094.0
54	12155.5
55	9217.0
56	7320.5
57	5424.0
58	4506.5
59	3589.0
60	3033.0
61	2477.0
62	1951.0
63	1425.0
64	1080.0
65	735.0
66	560.0
67	385.0
68	316.0
69	247.0
70	198.0
71	149.0
72	119.5
73	90.0
74	75.0
75	44.0
76	28.0
77	24.0
78	20.0
79	15.5
80	11.0
81	6.0
82	1.0
83	3.5
84	6.0
85	4.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	210907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.73342752966948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62733917370794	35.95897717951514
2	8.632004561712716	7.895423101177296
3	3.5280700844953605	4.840522125865903
4	1.8858535068166502	3.449861787422893
5	1.1850085532113421	2.709725139516469
6	0.7765279145715618	2.130796986349434
7	0.6220517339692084	1.991399052663022
8	0.5007516458452128	1.8320871284499805
9	0.4634285418070603	1.9074758068722233
>10	3.7105385931263286	28.448083752554442
>50	0.03421284536830646	1.0848383410697606
>100	0.0269555751386657	2.706406141095364
>500	0.0020735057798973618	0.569445300535307
>1k	0.0051837644497434035	4.474958156912763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2450	1.161649447386763	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	2068	0.9805269621207452	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	2037	0.9658285405415656	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC	1841	0.8728965847506247	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1042	0.494056622113064	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	694	0.32905498632098507	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	507	0.24039031421432197	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	497	0.2356488878984576	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTC	465	0.2204763236876917	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	462	0.2190538957929324	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	365	0.17306206052904835	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	307	0.14556178789703517	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.13655307789689294	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTA	269	0.1275443678967507	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	253	0.11995808579136776	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	239	0.11332008894915768	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	227	0.10763037737012048	No Hit
GAATCTATCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	222	0.10525966421218831	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.7414263158643386E-4	0.0	0.0	0.34422755053175097	0.0
2	4.7414263158643386E-4	0.0	0.0	1.1995808579136775	0.0
3	4.7414263158643386E-4	0.0	0.0	1.540015267392737	0.0
4	4.7414263158643386E-4	0.0	0.0	2.282048485825506	0.0
5	4.7414263158643386E-4	0.0	0.0	4.450776882701854	0.0
6	4.7414263158643386E-4	0.0	0.0	5.220784516398223	0.0
7	4.7414263158643386E-4	0.0	0.0	6.066180828516835	0.0
8	4.7414263158643386E-4	0.0	0.0	7.484815582223445	0.0
9	4.7414263158643386E-4	0.0	0.0	7.882147107492876	0.0
10	4.7414263158643386E-4	0.0	0.0	9.853158026997681	0.0
11	4.7414263158643386E-4	0.0	0.0	11.118644710701874	0.0
12	4.7414263158643386E-4	0.0	0.0	13.289269678104567	0.0
13	4.7414263158643386E-4	0.0	0.0	13.840223416008003	0.0
14	4.7414263158643386E-4	0.0	0.0	14.09483800916992	0.0
15	4.7414263158643386E-4	0.0	0.0	14.588420488651396	0.0
16	4.7414263158643386E-4	0.0	0.0	15.223297472345632	0.0
17	4.7414263158643386E-4	0.0	0.0	15.824036186565642	0.0
18	0.0014224278947593016	0.0	0.0	16.547103699734954	0.0
19	0.0014224278947593016	0.0	0.0	16.93732308553059	0.0
20	0.0014224278947593016	0.0	0.0	17.327542471326225	0.0
21	0.0014224278947593016	0.0	0.0	17.840564798702747	0.0
22	0.0014224278947593016	0.0	0.0	18.388199538185077	0.0
23	0.0014224278947593016	0.0	0.0	18.928247995562025	0.0
24	0.0014224278947593016	0.0	0.0	19.326053663463043	0.0
25	0.0014224278947593016	0.0	0.0	19.663169074520997	0.0
26	0.0014224278947593016	0.0	0.0	19.97942220978915	0.0
27	0.0014224278947593016	0.0	0.0	20.295201202425712	0.0
28	0.0014224278947593016	0.0	0.0	20.64132532348381	0.0
29	0.0014224278947593016	0.0	0.0	21.035337850332137	0.0
30	0.0018965705263457354	0.0	0.0	21.45874722033882	0.0
31	0.0018965705263457354	0.0	0.0	21.88737215929296	0.0
32	0.0018965705263457354	0.0	0.0	22.25909998245672	0.0
33	0.0018965705263457354	0.0	0.0	22.62656052193621	0.0
34	0.0018965705263457354	0.0	0.0	23.003503914047425	0.0
35	0.0018965705263457354	0.0	0.0	23.408895864053825	0.0
36	0.0018965705263457354	0.0	0.0	23.820925810902434	0.0
37	0.0018965705263457354	0.0	0.0	24.230110901961528	0.0
38	0.0018965705263457354	0.0	0.0	24.64308913407331	0.0
39	0.0018965705263457354	0.0	0.0	25.097791917764702	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	50	2.1827873E-11	45.0	5
AAATAGG	35	1.2056262E-7	45.0	2
GGTCAAG	20	7.0201774E-4	45.0	8
CTGCGGG	25	3.8799437E-5	45.0	3
CAGGGCA	20	7.0201774E-4	45.0	5
AATGCGG	20	7.0201774E-4	45.0	2
AATTGAC	20	7.0201774E-4	45.0	14
TACGAGG	25	3.8799437E-5	45.0	2
TACCTGA	35	1.2056262E-7	45.0	7
GGCGGGC	20	7.0201774E-4	45.0	4
GGCATGA	35	1.2056262E-7	45.0	8
TAGGCGG	25	3.8799437E-5	45.0	2
CCACGTG	25	3.8799437E-5	45.0	45
TGTACAC	20	7.0201774E-4	45.0	17
AGTACGG	20	7.0201774E-4	45.0	2
TTGGGAC	80	0.0	45.0	5
GGCACCG	65	0.0	44.999996	8
GGCCGAT	30	2.1570486E-6	44.999996	8
CAGGGTC	30	2.1570486E-6	44.999996	5
ACAGGGT	30	2.1570486E-6	44.999996	4
>>END_MODULE