FastQCFastQC Report
Sat 18 Jun 2016
SRR3549400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373785
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39001.043380552991693No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.45427184076407556No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC5680.15195901387161068No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG5040.13483687146354187No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5000.13376673756303759No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.1249381328838771No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4600.12306539855799457No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4470.11958746338135559No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC4320.11557446125446445No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4150.11102639217732119No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.10968872480169081No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC253.885738E-545.025
CGGGTCA207.0271717E-445.06
CGACGGT253.885738E-545.028
CATGCGG253.885738E-545.02
AAGCGTT207.0271717E-445.06
CGACAAT351.2089913E-745.020
ATCGTAG207.0271717E-445.01
AACGGGC406.7939254E-945.04
GCCGATT253.885738E-545.09
CTAACGG207.0271717E-445.02
CGAATAT551.8189894E-1245.014
GCGATTC207.0271717E-445.09
TCGTTGA600.044.99999624
CGTTGAT600.044.99999625
GCGATAT302.1615579E-644.9999969
GCTACGG302.1615579E-644.9999962
TCGAAAG302.1615579E-644.9999961
CGGTTTA1200.043.1249961
CGGTTTT8100.042.7777751
CGTTTTT22050.041.9387741
Produced by FastQC (version 0.11.5)