##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549397_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1141572
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36920930085881	33.0	31.0	34.0	31.0	34.0
2	32.71377977035176	34.0	31.0	34.0	31.0	34.0
3	32.66806386281373	34.0	31.0	34.0	31.0	34.0
4	36.24209861489245	37.0	37.0	37.0	35.0	37.0
5	36.2818805997344	37.0	37.0	37.0	35.0	37.0
6	36.30649490351901	37.0	37.0	37.0	35.0	37.0
7	36.34666670170607	37.0	37.0	37.0	35.0	37.0
8	36.12219991380307	37.0	37.0	37.0	35.0	37.0
9	38.02690500467776	39.0	39.0	39.0	35.0	39.0
10	37.83621094420676	39.0	38.0	39.0	35.0	39.0
11	37.902625502377425	39.0	38.0	39.0	35.0	39.0
12	37.74643474086611	39.0	37.0	39.0	35.0	39.0
13	37.716890393247205	39.0	38.0	39.0	35.0	39.0
14	39.12749611938625	41.0	39.0	41.0	35.0	41.0
15	39.11191234543244	41.0	39.0	41.0	35.0	41.0
16	39.12957395591342	41.0	39.0	41.0	35.0	41.0
17	38.986237398955126	41.0	38.0	41.0	35.0	41.0
18	38.86866093422053	40.0	38.0	41.0	35.0	41.0
19	38.796748693906295	40.0	37.0	41.0	35.0	41.0
20	38.692151699586184	40.0	37.0	41.0	35.0	41.0
21	38.67155028329356	40.0	37.0	41.0	35.0	41.0
22	38.664402245324865	40.0	37.0	41.0	35.0	41.0
23	38.64208477432873	40.0	37.0	41.0	35.0	41.0
24	38.56657135949375	40.0	37.0	41.0	35.0	41.0
25	38.48371193406986	40.0	37.0	41.0	35.0	41.0
26	38.455876633274116	40.0	36.0	41.0	35.0	41.0
27	38.44392381733259	40.0	36.0	41.0	35.0	41.0
28	38.31245422978139	40.0	36.0	41.0	35.0	41.0
29	38.27639956130669	40.0	36.0	41.0	35.0	41.0
30	38.106800096708746	40.0	36.0	41.0	35.0	41.0
31	37.95513905386607	40.0	36.0	41.0	34.0	41.0
32	37.68697287599906	40.0	36.0	41.0	34.0	41.0
33	37.41770996485548	40.0	36.0	41.0	33.0	41.0
34	37.102887071511915	40.0	36.0	41.0	33.0	41.0
35	36.91563563226848	40.0	36.0	41.0	32.0	41.0
36	36.802978699547644	40.0	35.0	41.0	31.0	41.0
37	36.72879765796638	40.0	35.0	41.0	31.0	41.0
38	36.64287929276471	40.0	35.0	41.0	31.0	41.0
39	36.564979694666654	40.0	35.0	41.0	31.0	41.0
40	36.44047769216484	40.0	35.0	41.0	30.0	41.0
41	36.290054416191005	40.0	35.0	40.0	30.0	41.0
42	36.25395857641918	40.0	35.0	40.0	30.0	41.0
43	36.14143041349998	39.0	35.0	40.0	30.0	41.0
44	36.04647801452734	39.0	35.0	40.0	29.0	41.0
45	35.9877607369487	39.0	35.0	40.0	29.0	41.0
46	35.876360842767696	39.0	35.0	40.0	29.0	41.0
47	35.793364763676756	39.0	35.0	40.0	29.0	41.0
48	35.70091768193333	38.0	35.0	40.0	28.0	41.0
49	35.59328539943166	38.0	35.0	40.0	27.0	41.0
50	35.517132515513694	38.0	35.0	40.0	27.0	41.0
51	35.48484633470338	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	8.0
14	10.0
15	24.0
16	44.0
17	100.0
18	213.0
19	416.0
20	735.0
21	1283.0
22	2034.0
23	3141.0
24	5150.0
25	8812.0
26	14287.0
27	18579.0
28	18068.0
29	15712.0
30	14572.0
31	15463.0
32	17956.0
33	23000.0
34	44703.0
35	89578.0
36	56032.0
37	78607.0
38	207394.0
39	505637.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.97084020981594	23.83958261064567	29.007368786200082	18.18220839333831
2	33.907979522973584	25.22101102689975	28.483967721703056	12.387041728423611
3	27.475621336192546	25.446577176034452	34.97711927061981	12.100682217153189
4	25.013752965209378	25.12859460463291	36.6730263180947	13.184626112063015
5	23.196346791967567	30.652118307036265	32.820531687883026	13.331003213113146
6	20.370419036206215	40.711142179380715	29.844022102854662	9.074416681558413
7	80.84387143342688	3.750792766465891	13.18226095244102	2.223074847666201
8	79.61959473427869	5.534123121450071	12.378019082458225	2.4682630618130084
9	72.81862204048453	8.828440080870939	13.533706152568564	4.819231726075972
10	25.83840528674494	46.58024198210888	18.798463872624765	8.782888858521408
11	28.088110079784716	22.941610340828262	33.937237423482706	15.033042155904313
12	28.62289894986913	21.28485982487307	33.57335323571356	16.518887989544243
13	21.447705444772648	26.841846156002426	34.436811694750745	17.273636704474182
14	15.638085026612424	30.14045544214469	34.86560637436797	19.355853156874904
15	15.11774990977354	28.522335866682084	40.7129817479756	15.646932475568779
16	18.529974456276083	28.01978324626042	37.38958208505465	16.060660212408852
17	17.67256029405066	28.257262792009612	34.13669921827094	19.93347769566878
18	16.582221708310996	29.746086974803166	36.51280865333067	17.15888266355517
19	19.970444264575516	28.35300795744815	33.679172229171705	17.99737554880463
20	21.07637538411944	27.699260318227847	37.0357717253051	14.188592572347606
21	19.469293220226145	29.486007014888244	36.3777317593634	14.666968005522211
22	19.46324892341438	25.530496543363014	35.696478189724345	19.309776343498264
23	17.7281853444198	27.799210211883263	36.85926073870067	17.613343704996268
24	16.24505506441994	27.81147400251583	39.67178592327072	16.27168500979351
25	15.261148661670049	31.037113734394328	36.03898834239102	17.662749261544608
26	15.655341932002537	32.566758820293416	34.092023980966594	17.685875266737447
27	16.678229669263086	32.07576920246817	34.61104512023771	16.634956008031033
28	13.825233975605569	31.36166619363474	37.64256656610358	17.170533264656108
29	16.211329640180384	27.193378954634483	37.556369637657546	19.038921767527583
30	16.2439162838612	30.491725445263196	36.22977788523195	17.034580385643654
31	19.721314117725385	28.61878182015677	33.96264098979302	17.69726307232483
32	18.672584821631926	28.103439818075426	35.99115955892401	17.232815801368638
33	19.212279207969363	28.84478596181406	34.06775919521502	17.875175635001558
34	16.244967465915423	28.82183515363026	35.21748956701811	19.715707813436207
35	18.313080559088696	27.108933996278818	35.43131751654735	19.146667928085133
36	19.751097609261613	29.665321153637265	32.536537336234595	18.047043900866523
37	16.523618308788233	31.677896794945916	34.607453581552456	17.191031314713396
38	16.06609131968899	30.56513299204956	33.83176882404263	19.537006864218814
39	18.228810797742064	29.341995073460104	34.92166941725971	17.507524711538125
40	18.13490520089841	28.642871408899307	34.72028045537206	18.50194293483022
41	16.05776946175975	29.941694435392595	33.752054184930955	20.248481917916695
42	15.97735403461192	29.299860192786788	33.64824995707673	21.074535815524555
43	18.429060979070965	28.216529487408593	32.71129635274866	20.643113180771778
44	16.4708840090682	30.445385836373003	32.848826004842444	20.234904149716357
45	15.87197303367637	31.47887299268027	32.738364290644824	19.91078968299853
46	17.71469517472398	32.65689768144278	31.40529024888487	18.22311689494837
47	17.344153500611437	28.670727733336136	34.55673404743634	19.428384718616083
48	17.33986117389004	28.334437074490264	35.93798726668138	18.387714484938314
49	17.202594317309817	29.288997978226515	34.75242910653029	18.755978597933375
50	15.915597088926498	29.654634136086027	35.05578272767727	19.3739860473102
51	17.06383828615278	28.199009786504924	33.23574859929991	21.501403328042386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1089.0
1	2034.5
2	2980.0
3	18584.0
4	34188.0
5	22391.0
6	10594.0
7	10175.5
8	9757.0
9	9632.5
10	9508.0
11	9335.5
12	9163.0
13	8741.0
14	8319.0
15	7720.5
16	7122.0
17	7305.5
18	7489.0
19	7331.0
20	7173.0
21	7872.5
22	8572.0
23	9690.0
24	10808.0
25	13055.5
26	18879.0
27	22455.0
28	28185.5
29	33916.0
30	42332.0
31	50748.0
32	54708.5
33	58669.0
34	62249.0
35	65829.0
36	68219.0
37	70609.0
38	75293.0
39	79977.0
40	85831.5
41	91686.0
42	91690.5
43	91695.0
44	91175.0
45	90655.0
46	94079.0
47	97503.0
48	85069.0
49	72635.0
50	64132.5
51	55630.0
52	44866.0
53	34102.0
54	28126.0
55	22150.0
56	19116.0
57	16082.0
58	15129.0
59	14176.0
60	12994.5
61	11813.0
62	9884.5
63	7956.0
64	6278.0
65	4600.0
66	3637.5
67	2675.0
68	2131.5
69	1588.0
70	1320.5
71	1053.0
72	827.5
73	602.0
74	435.0
75	209.5
76	151.0
77	127.0
78	103.0
79	108.0
80	113.0
81	71.0
82	29.0
83	24.0
84	19.0
85	13.0
86	7.0
87	7.0
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1141572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.466718208653468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55714747630009	16.4343071173206
2	8.917902856581208	3.8287621526874913
3	3.263587958005653	2.1017556913098643
4	1.6702194690326009	1.4341652275331862
5	1.1080001116983091	1.1892563086492087
6	0.8026645349610173	1.0338344032852824
7	0.6324665057479342	0.9503886178711828
8	0.5097059253268189	0.8753370774617429
9	0.4153201518870408	0.8024004600240836
>10	4.1061262554382365	18.8603963318371
>50	1.016624659208893	15.8462522485034
>100	0.9883509377710049	31.976624645269403
>500	0.008605045478105765	1.318124031176379
>1k	0.0028683484927019215	1.5513136745963712
>5k	0.0	0.0
>10k+	4.097640703859888E-4	1.7970820124747031
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20430	1.7896374473094996	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	4239	0.3713300606532045	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	3130	0.2741833191423756	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	2826	0.24755337376880301	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2327	0.2038417200141559	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2241	0.1963082486255795	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1833	0.16056805878210048	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7519700903666173E-4	0.0	0.0	0.06657486343393146	0.0
2	1.7519700903666173E-4	0.0	0.0	0.3139530401936978	0.0
3	1.7519700903666173E-4	0.0	0.0	0.43983209118653926	0.0
4	1.7519700903666173E-4	0.0	0.0	0.6466521603543184	0.0
5	1.7519700903666173E-4	0.0	0.0	1.3109116201168214	0.0
6	1.7519700903666173E-4	0.0	0.0	1.6371284509430855	0.0
7	1.7519700903666173E-4	0.0	0.0	1.9474899524515317	0.0
8	1.7519700903666173E-4	0.0	0.0	2.459590809865694	0.0
9	1.7519700903666173E-4	0.0	0.0	2.6056175168977513	0.0
10	1.7519700903666173E-4	0.0	0.0	3.3141142214420114	0.0
11	1.7519700903666173E-4	0.0	0.0	3.8356757173441536	0.0
12	1.7519700903666173E-4	0.0	0.0	4.512549361757296	0.0
13	1.7519700903666173E-4	0.0	0.0	4.6897611363978795	0.0
14	2.627955135549926E-4	0.0	0.0	4.764745456265571	0.0
15	2.627955135549926E-4	0.0	0.0	4.8984207741605434	0.0
16	2.627955135549926E-4	0.0	0.0	5.106379623887061	0.0
17	2.627955135549926E-4	0.0	0.0	5.340530426464559	0.0
18	2.627955135549926E-4	0.0	0.0	5.616465715697301	0.0
19	2.627955135549926E-4	0.0	0.0	5.838352727642234	0.0
20	2.627955135549926E-4	0.0	0.0	6.066459233407968	0.0
21	2.627955135549926E-4	0.0	0.0	6.331269512566881	0.0
22	2.627955135549926E-4	0.0	0.0	6.640842627534663	0.0
23	2.627955135549926E-4	0.0	0.0	6.934210019166553	0.0
24	2.627955135549926E-4	0.0	0.0	7.165032078572355	0.0
25	2.627955135549926E-4	0.0	0.0	7.371063761199469	0.0
26	2.627955135549926E-4	0.0	0.0	7.576131860276881	0.0
27	2.627955135549926E-4	0.0	0.0	7.776382041605785	0.0
28	2.627955135549926E-4	0.0	0.0	8.005189335407666	0.0
29	3.5039401807332346E-4	0.0	0.0	8.249151170491217	0.0
30	3.5039401807332346E-4	0.0	0.0	8.519918147957378	0.0
31	3.5039401807332346E-4	0.0	0.0	8.780874092917486	0.0
32	3.5039401807332346E-4	0.0	0.0	9.039026885733007	0.0
33	3.5039401807332346E-4	0.0	0.0	9.2745792643828	0.0
34	3.5039401807332346E-4	0.0	0.0	9.52589937384589	0.0
35	4.379925225916543E-4	0.0	0.0	9.812609279134387	0.0
36	4.379925225916543E-4	0.0	0.0	10.074266012130641	0.0
37	4.379925225916543E-4	0.0	0.0	10.350288899867902	0.0
38	4.379925225916543E-4	0.0	0.0	10.628589348722638	0.0
39	4.379925225916543E-4	0.0	0.0	10.931417378842509	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	20	7.033265E-4	45.000004	6
AGCGTAT	30	2.1654887E-6	45.000004	44
GCGAACG	20	7.033265E-4	45.000004	1
CGGCCCT	35	1.2119199E-7	45.000004	37
TCGGGTG	20	7.033265E-4	45.000004	5
GGTCCAC	20	7.033265E-4	45.000004	42
CGCTAGC	20	7.033265E-4	45.000004	26
GGACGTA	35	1.2119199E-7	45.000004	8
GTTCCGT	30	2.1654887E-6	45.000004	19
TCATCGG	20	7.033265E-4	45.000004	42
CGCAATC	20	7.033265E-4	45.000004	20
ATCGGAT	20	7.033265E-4	45.000004	44
TTCGCGG	60	0.0	45.000004	2
CATCACG	35	1.2119199E-7	45.000004	14
GCGTAGT	20	7.033265E-4	45.000004	3
GCGTAAG	70	0.0	45.000004	1
GTCGGAA	20	7.033265E-4	45.000004	13
TACCGGG	80	0.0	45.000004	3
CGTAATC	25	3.8907896E-5	45.0	19
CGTAAGT	25	3.8907896E-5	45.0	16
>>END_MODULE