##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	809060
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.794997898796133	31.0	31.0	34.0	30.0	34.0
2	32.06078411984278	33.0	31.0	34.0	30.0	34.0
3	31.781633006204732	33.0	31.0	34.0	30.0	34.0
4	35.66353051689615	37.0	35.0	37.0	33.0	37.0
5	28.47166959187205	35.0	32.0	37.0	0.0	37.0
6	32.013111512125185	37.0	32.0	37.0	17.0	37.0
7	34.94642918942971	37.0	33.0	37.0	32.0	37.0
8	35.56981929646751	37.0	35.0	37.0	33.0	37.0
9	37.59611524485205	39.0	37.0	39.0	35.0	39.0
10	37.2560514671347	39.0	37.0	39.0	34.0	39.0
11	37.28245741972165	39.0	37.0	39.0	34.0	39.0
12	37.09338120782142	39.0	37.0	39.0	33.0	39.0
13	36.666417818208785	39.0	35.0	39.0	32.0	39.0
14	38.13459446765382	40.0	37.0	41.0	33.0	41.0
15	38.274047660247696	40.0	37.0	41.0	33.0	41.0
16	38.27647764071886	40.0	37.0	41.0	33.0	41.0
17	38.20589449484587	40.0	37.0	41.0	33.0	41.0
18	38.04623513707265	40.0	37.0	41.0	33.0	41.0
19	38.05742713766593	40.0	37.0	41.0	34.0	41.0
20	38.03237707957383	40.0	36.0	41.0	34.0	41.0
21	37.97899661335377	40.0	36.0	41.0	34.0	41.0
22	37.827084517835516	40.0	35.0	41.0	33.0	41.0
23	37.81914691123032	40.0	35.0	41.0	33.0	41.0
24	37.72961585049317	40.0	35.0	41.0	33.0	41.0
25	37.616536474427114	39.0	35.0	41.0	33.0	41.0
26	37.54065582280671	39.0	35.0	41.0	33.0	41.0
27	37.420123353026966	39.0	35.0	41.0	33.0	41.0
28	37.38976342916471	39.0	35.0	41.0	33.0	41.0
29	37.346739426000546	39.0	35.0	41.0	33.0	41.0
30	37.14922131856723	39.0	35.0	41.0	32.0	41.0
31	37.031935826761924	39.0	35.0	40.0	32.0	41.0
32	36.81857340617507	39.0	35.0	40.0	31.0	41.0
33	36.622202308852245	39.0	35.0	40.0	31.0	41.0
34	36.31507181173213	39.0	35.0	40.0	30.0	41.0
35	36.07273502583245	39.0	35.0	40.0	30.0	41.0
36	35.974131708402346	39.0	35.0	40.0	29.0	41.0
37	35.880790052653694	39.0	35.0	40.0	29.0	41.0
38	35.7261204360616	38.0	35.0	40.0	28.0	41.0
39	35.631693570316166	38.0	35.0	40.0	27.0	41.0
40	35.4717103799471	38.0	35.0	40.0	27.0	41.0
41	35.332712036190145	38.0	35.0	40.0	26.0	41.0
42	35.289302400316416	38.0	35.0	40.0	26.0	41.0
43	35.138531134897285	38.0	35.0	40.0	25.0	41.0
44	34.969883568585765	38.0	34.0	40.0	24.0	41.0
45	34.895021382839346	38.0	34.0	40.0	24.0	41.0
46	34.766866487034335	38.0	34.0	40.0	24.0	41.0
47	34.62964922255457	38.0	34.0	40.0	24.0	41.0
48	34.52876671693076	38.0	34.0	40.0	23.0	40.0
49	34.455406273947546	37.0	34.0	40.0	24.0	40.0
50	34.35742219365684	37.0	34.0	40.0	24.0	40.0
51	34.639648480953205	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	4.0
14	3.0
15	11.0
16	48.0
17	108.0
18	237.0
19	531.0
20	877.0
21	1461.0
22	2335.0
23	3513.0
24	5666.0
25	8656.0
26	12217.0
27	14108.0
28	14273.0
29	14808.0
30	16463.0
31	19739.0
32	25143.0
33	35711.0
34	51339.0
35	69371.0
36	67272.0
37	112452.0
38	163166.0
39	169540.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.33893654364324	24.52586952759993	29.157911650557438	17.97728227819939
2	31.890959879366175	25.55051541294836	29.96366153313722	12.594863174548241
3	28.34919536251947	25.38649791115616	33.9314760339159	12.332830692408473
4	25.639260376239093	26.33068004845129	34.60423207178701	13.425827503522605
5	18.1347489679381	45.55150421476776	25.384520307517366	10.929226509776777
6	20.790423454379155	41.30892640842459	28.728771660939856	9.171878476256397
7	81.28148715793637	4.858848540281314	11.23365386992312	2.6260104318591946
8	80.20529997775195	5.8345487355696735	11.179764170766074	2.7803871159122933
9	73.43831112649248	9.988875979531803	12.172150396756729	4.4006624972189945
10	25.378093095691295	48.54658492571626	17.257805354361853	8.817516624230588
11	28.224606333275652	23.8737547277087	32.9444046177045	14.957234321311152
12	29.258275035226067	22.54023187402665	32.33715670036833	15.864336390378957
13	21.296689985909577	28.456480359923862	32.637752453464515	17.60907720070205
14	15.850987565817121	31.012162262378563	33.64558870788322	19.491261463921095
15	15.814772699181766	29.62400810817492	39.070526289768374	15.490692902874942
16	19.114157170049193	29.11601117346056	36.31189281388278	15.457938842607472
17	18.244505969890984	28.967072899414138	32.66828170963835	20.120139421056535
18	17.294638222134328	30.85914518082713	35.181074333176774	16.665142263861764
19	20.799817071663412	29.058289867253357	32.32306627444195	17.81882678664129
20	21.445257459273726	28.58440659530814	35.61688873507528	14.353447210342868
21	19.886287790769536	30.13979185721702	35.08948656465528	14.88443378735817
22	20.227795219143204	26.42090821447112	34.14122561985514	19.210070946530543
23	18.162559019108595	28.557090945047335	35.64037277828591	17.639977257558154
24	16.497045954564555	28.632610684003655	39.04160383655106	15.828739524880724
25	15.611326724841174	31.583195313079376	35.40973475391195	17.395743208167502
26	16.240699082886312	34.05767186611624	32.370528761772924	17.331100289224533
27	16.7908436951524	33.34017254591748	33.46822238152918	16.400761377400936
28	14.310805131881443	32.355202333572294	36.5301708155143	16.803821719031966
29	16.46157268929375	28.433737918077767	36.49037154228364	18.614317850344843
30	16.266407930190592	31.628432996316715	35.06266531530418	17.042493758188513
31	20.415543964600893	29.70397745531852	33.024744765530365	16.85573381455022
32	18.903913183200256	29.0018045633204	35.286876127852075	16.80740612562727
33	19.484092650730478	29.777643190863472	33.392949843027715	17.345314315378342
34	16.569722888290116	29.78320520109757	34.492003065285644	19.155068845326674
35	18.704793216819517	27.87593009171137	35.16920871134403	18.250067980125085
36	20.498356116975256	30.27167329987887	31.91469112303167	17.31527946011421
37	16.71396435369441	32.903863743109284	33.50690925271302	16.87526265048328
38	16.505697970484263	31.937062764195485	32.67507972214669	18.88215954317356
39	18.67710676587645	30.385509109336763	33.612340246706054	17.325043878080738
40	18.707512421822855	29.74352952809433	33.493807628606035	18.055150421476775
41	16.44179665290584	30.689071267891133	33.207920302573356	19.66121177662967
42	16.105727634538848	30.050799693471436	32.993968308901685	20.849504363088027
43	18.840135465849254	28.850517884952907	32.01839171384076	20.29095493535708
44	16.525844807554446	31.835834177934885	32.07450621709144	19.563814797419226
45	16.10461523249203	33.29555286381727	31.64029861814946	18.959533285541244
46	17.6295948384545	34.23293698860406	30.404914345042393	17.732553827899046
47	18.0183175537043	29.725978295800065	33.13215336316219	19.12355078733345
48	17.651101278026353	29.527352730329028	35.09937458284923	17.722171408795393
49	17.51464662694979	30.957160161174695	33.49269522655921	18.035497985316294
50	16.295948384545028	31.423380219019602	33.71764764047166	18.563023755963712
51	17.542703878575136	29.799026030207898	32.34877512174623	20.30949496947074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	673.0
1	1218.0
2	1763.0
3	10242.5
4	18722.0
5	13029.0
6	7336.0
7	6851.0
8	6366.0
9	6180.0
10	5994.0
11	5925.0
12	5856.0
13	5561.5
14	5267.0
15	4948.0
16	4629.0
17	4749.5
18	4870.0
19	5011.0
20	5152.0
21	5581.5
22	6011.0
23	6981.0
24	7951.0
25	9808.0
26	14404.5
27	17144.0
28	21668.0
29	26192.0
30	32277.5
31	38363.0
32	41531.0
33	44699.0
34	47681.0
35	50663.0
36	52535.0
37	54407.0
38	58186.0
39	61965.0
40	65789.5
41	69614.0
42	69757.5
43	69901.0
44	68638.0
45	67375.0
46	65413.0
47	63451.0
48	54815.0
49	46179.0
50	39993.5
51	33808.0
52	27183.5
53	20559.0
54	16911.5
55	13264.0
56	11609.5
57	9955.0
58	9484.0
59	9013.0
60	8467.0
61	7921.0
62	6509.0
63	5097.0
64	4013.5
65	2930.0
66	2359.0
67	1788.0
68	1413.5
69	1039.0
70	850.0
71	661.0
72	528.5
73	396.0
74	290.0
75	136.5
76	89.0
77	74.0
78	59.0
79	54.0
80	49.0
81	34.5
82	20.0
83	14.0
84	8.0
85	7.0
86	6.0
87	4.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	809060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.027276242711178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69749175306765	17.94777268988597
2	7.890761755719228	3.791870249401731
3	3.5390398824933036	2.5510046667191615
4	2.1301258253481814	2.047244865494956
5	1.5370062302095095	1.846503664000601
6	1.1425130900706415	1.6470886575624515
7	0.8750048021952765	1.4716787467231336
8	0.6872645003014735	1.321047520044189
9	0.5884381380471095	1.2724709125144065
>10	5.159691691592511	26.22117070517825
>50	1.2656654381544163	22.075310909834233
>100	0.48120557839976896	15.125298837692663
>500	0.0021059325094081793	0.352427761289072
>1k	0.003158898764112269	1.2689170400181915
>5k	5.264831273520448E-4	1.0601927736409593
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8381	1.035893506044051	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2066	0.25535806985884857	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1876	0.23187402664820905	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1722	0.21283959162484858	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	1519	0.18774874545769163	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1458	0.18020913158480212	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	1390	0.17180431611994168	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	940	0.11618421377895334	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.652015919709292E-4	0.0	0.0	0.03225965935777322	0.0
2	8.652015919709292E-4	0.0	0.0	0.15351148246112772	0.0
3	8.652015919709292E-4	0.0	0.0	0.20270437297604627	0.0
4	8.652015919709292E-4	0.0	0.0	0.2905841346748078	0.0
5	8.652015919709292E-4	0.0	0.0	0.6309791610016563	0.0
6	8.652015919709292E-4	0.0	0.0	0.7956146639309817	0.0
7	8.652015919709292E-4	0.0	0.0	0.9644525745927373	0.0
8	8.652015919709292E-4	0.0	0.0	1.234024670605394	0.0
9	8.652015919709292E-4	0.0	0.0	1.306701604330952	0.0
10	8.652015919709292E-4	0.0	0.0	1.6993795268583294	0.0
11	8.652015919709292E-4	0.0	0.0	1.9274219464563815	0.0
12	9.888018193953477E-4	0.0	0.0	2.272266580970509	0.0
13	9.888018193953477E-4	0.0	0.0	2.362989147900032	0.0
14	9.888018193953477E-4	0.0	0.0	2.4006872172644798	0.0
15	0.0011124020468197661	0.0	0.0	2.4659481373445726	0.0
16	0.0011124020468197661	0.0	0.0	2.581267149531555	0.0
17	0.0011124020468197661	0.0	0.0	2.716485798333869	0.0
18	0.0011124020468197661	0.0	0.0	2.8737052876177294	0.0
19	0.0011124020468197661	0.0	0.0	2.9790126813833337	0.0
20	0.0011124020468197661	0.0	0.0	3.1000173040318395	0.0
21	0.0011124020468197661	0.0	0.0	3.245865572392653	0.0
22	0.0011124020468197661	0.0	0.0	3.4128494796430426	0.0
23	0.0011124020468197661	0.0	0.0	3.5824289916693446	0.0
24	0.0011124020468197661	0.0	0.0	3.717524040244234	0.0
25	0.001359602501668603	0.0	0.0	3.836427459026525	0.0
26	0.001359602501668603	0.0	0.0	3.953476874397449	0.0
27	0.001359602501668603	0.0	0.0	4.069290287494129	0.0
28	0.001359602501668603	0.0	0.0	4.199812127654315	0.0
29	0.001359602501668603	0.0	0.0	4.348874001928164	0.0
30	0.001359602501668603	0.0	0.0	4.521790720094925	0.0
31	0.001359602501668603	0.0	0.0	4.6923590339406225	0.0
32	0.001359602501668603	0.0	0.0	4.847477319358267	0.0
33	0.0014832027290930215	0.0	0.0	4.997404395224088	0.0
34	0.0014832027290930215	0.0	0.0	5.159197092922651	0.0
35	0.0014832027290930215	0.0	0.0	5.330754208587744	0.0
36	0.0014832027290930215	0.0	0.0	5.505895730848144	0.0
37	0.0014832027290930215	0.0	0.0	5.68981286925568	0.0
38	0.00160680295651744	0.0	0.0	5.893506044051121	0.0
39	0.00160680295651744	0.0	0.0	6.091884409067313	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	20	7.032046E-4	45.000004	43
GTCGACA	30	2.164701E-6	45.000004	43
GCGAACG	20	7.032046E-4	45.000004	1
ATTACCG	20	7.032046E-4	45.000004	24
CGTTCGA	30	2.164701E-6	45.000004	38
TATTGCG	40	6.8102963E-9	45.000004	1
CTAACCG	20	7.032046E-4	45.000004	26
CCGTACA	20	7.032046E-4	45.000004	30
TGACCGA	20	7.032046E-4	45.000004	34
GCGATAT	20	7.032046E-4	45.000004	9
GCGGCTA	20	7.032046E-4	45.000004	24
CGAGACT	20	7.032046E-4	45.000004	12
AACTCGA	20	7.032046E-4	45.000004	38
ACGTAAC	20	7.032046E-4	45.000004	10
CAATCGT	20	7.032046E-4	45.000004	41
CTAGGCG	20	7.032046E-4	45.000004	1
GCGTATG	65	0.0	45.000004	1
CCGTTCG	30	2.164701E-6	45.000004	37
TCGCGGG	40	6.8102963E-9	45.000004	3
TACGTGT	20	7.032046E-4	45.000004	23
>>END_MODULE