FastQCFastQC Report
Sat 18 Jun 2016
SRR3549392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740489
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.23592517917214165No Hit
AAGGAAAGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15770.21296737696306092No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15230.20567489861429405No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13310.17974608670756756No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG12240.16529617590538145No Hit
GAATCTATCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC10390.14031268526608767No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7840.10587598195246654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.0316583E-445.00000436
AGCGTAA302.1644519E-645.0000041
AACCGCG302.1644519E-645.00000425
CGTATAG600.045.0000042
CTGTACG302.1644519E-645.00000425
CGACGAA207.0316583E-445.00000417
GGGTCGA207.0316583E-445.00000411
GCGCGAG207.0316583E-445.0000042
ACCGCGT302.1644519E-645.00000426
ATTACGC302.1644519E-645.00000429
CGCGAAG207.0316583E-445.0000042
CTCGCTA207.0316583E-445.0000041
TCGTATA302.1644519E-645.0000041
CCGAATA406.8102963E-945.0000041
ATTGCGG207.0316583E-445.0000042
TATACGC207.0316583E-445.00000413
TAAGCGC302.1644519E-645.00000419
ACCCCGA302.1644519E-645.00000424
TATCGTC207.0316583E-445.00000425
GTGCGAA207.0316583E-445.0000041