##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549392_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740489
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5416852917464	31.0	31.0	33.0	30.0	34.0
2	31.875394502821784	31.0	31.0	34.0	30.0	34.0
3	31.852651423586305	31.0	31.0	34.0	30.0	34.0
4	35.665847838387876	37.0	35.0	37.0	35.0	37.0
5	35.57329413401144	37.0	35.0	37.0	33.0	37.0
6	35.7406403066082	37.0	35.0	37.0	35.0	37.0
7	4.8668555508589595	0.0	0.0	0.0	0.0	35.0
8	19.579286120388012	17.0	17.0	17.0	17.0	35.0
9	31.537883749792368	32.0	32.0	32.0	27.0	37.0
10	34.910561804429236	35.0	34.0	37.0	32.0	37.0
11	36.65060790909791	37.0	35.0	39.0	33.0	39.0
12	37.09076704718098	39.0	37.0	39.0	34.0	39.0
13	36.97802128053219	39.0	37.0	39.0	33.0	39.0
14	38.40148739549136	40.0	38.0	41.0	34.0	41.0
15	38.52595919723318	40.0	38.0	41.0	34.0	41.0
16	38.545295068529036	40.0	38.0	41.0	34.0	41.0
17	38.47584231501076	40.0	38.0	41.0	34.0	41.0
18	38.35046030393429	40.0	38.0	41.0	34.0	41.0
19	38.318675902005296	40.0	38.0	41.0	34.0	41.0
20	38.280795528360315	40.0	37.0	41.0	34.0	41.0
21	38.20464044705593	40.0	37.0	41.0	34.0	41.0
22	38.08545974349383	40.0	37.0	40.0	34.0	41.0
23	37.998660344718154	39.0	37.0	40.0	34.0	41.0
24	37.849134828471456	39.0	37.0	40.0	33.0	41.0
25	37.7208439288092	39.0	36.0	40.0	33.0	41.0
26	37.50535794589791	39.0	36.0	40.0	33.0	41.0
27	37.33329732109458	39.0	36.0	40.0	33.0	41.0
28	37.19418249292022	39.0	36.0	40.0	32.0	41.0
29	37.07678304471775	38.0	36.0	40.0	32.0	41.0
30	36.85683919680103	38.0	36.0	40.0	32.0	41.0
31	36.68287847625015	38.0	35.0	40.0	31.0	41.0
32	36.399017406065454	38.0	35.0	40.0	31.0	40.0
33	36.22774004745513	38.0	35.0	40.0	30.0	40.0
34	35.99375007596332	38.0	35.0	40.0	30.0	40.0
35	35.754293446627834	38.0	35.0	40.0	30.0	40.0
36	35.62262910049981	38.0	34.0	40.0	30.0	40.0
37	35.51186715805366	38.0	34.0	39.0	30.0	40.0
38	35.17707352843864	37.0	34.0	39.0	29.0	40.0
39	35.02424343913279	37.0	34.0	39.0	29.0	40.0
40	34.70753380536375	37.0	33.0	39.0	28.0	40.0
41	34.29001376117674	36.0	33.0	38.0	27.0	40.0
42	34.08608500598928	36.0	33.0	38.0	26.0	40.0
43	33.96010474159643	36.0	33.0	38.0	26.0	40.0
44	33.651492459712436	36.0	32.0	38.0	25.0	40.0
45	33.436311680524625	35.0	32.0	38.0	24.0	39.0
46	33.26492088336221	35.0	31.0	38.0	24.0	39.0
47	33.03124691926551	35.0	31.0	38.0	24.0	39.0
48	32.69585098495724	35.0	31.0	38.0	24.0	39.0
49	32.46582190957597	34.0	31.0	38.0	24.0	39.0
50	32.31613028687799	34.0	30.0	37.0	23.0	39.0
51	32.173866188424135	34.0	30.0	37.0	23.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	6.0
15	7.0
16	36.0
17	67.0
18	151.0
19	310.0
20	676.0
21	1174.0
22	2113.0
23	3421.0
24	4988.0
25	6542.0
26	7773.0
27	9258.0
28	11533.0
29	14753.0
30	19759.0
31	27289.0
32	38743.0
33	54169.0
34	76433.0
35	128312.0
36	210343.0
37	98819.0
38	21427.0
39	2378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.83785039345622	25.83130877028558	30.60383071186743	13.727010124390773
2	28.691985971432395	27.06252219816905	31.244623485291477	13.000868345107083
3	29.921713894467032	27.03672843215767	30.42719068075285	12.614366992622442
4	26.78810893882286	28.22162111793693	31.14158346714131	13.848686476098901
5	23.544846716156485	34.14716491399602	28.301028104401283	14.00696026544621
6	21.830033937033498	44.45211205028029	25.07910313319982	8.638750879486395
7	11.995181562454	86.87542961475458	0.8451172130848669	0.2842716097065588
8	86.84774520620833	5.362267366564527	5.672872925863855	2.117114501363288
9	79.42602793559391	10.241880703156967	6.6149530918082515	3.7171382694408694
10	26.753807281404583	52.471677499598236	12.105108921266892	8.669406297730283
11	30.235425509359352	25.31111198140688	29.147360730544275	15.306101778689488
12	31.260288809151792	24.11028387997661	28.466594372097358	16.162832938774244
13	22.150767938483895	30.63327071705319	28.754647266873647	18.461314077589268
14	16.31529975462161	33.43196185223548	29.89565003666496	20.35708835647795
15	16.553250622223963	31.8154624849255	35.7335490466435	15.897737846207033
16	20.011640956178958	31.109982727629987	33.10515078549445	15.773225530696608
17	19.14640190468731	30.868520666748594	28.809610946280095	21.175466482284005
18	18.170155127219985	33.09907372020381	31.677850717566365	17.05292043500984
19	22.185744825378904	31.03314161317724	28.31966443795924	18.461449123484616
20	22.799258327942752	30.483909956798815	31.995478663423764	14.721353051834665
21	21.06905031675015	32.289473577595345	31.288918538965465	15.35255756668904
22	21.437320473362874	28.00932897045061	30.462167567647864	20.091182988538655
23	19.18732081097761	30.407879117718156	32.044635369330265	18.360164701973964
24	17.17864816357839	30.447177473264286	36.14138765059305	16.232786712564266
25	16.401594081748684	33.52257764801368	32.047336287237215	18.02849198300042
26	17.012541712300923	36.5361268026939	28.466054188515965	17.985277296489212
27	17.527066573575027	35.85346980171211	29.84338727516547	16.776076349547395
28	14.91892519672811	34.609291967875286	33.22372108160959	17.248061753787024
29	17.132597513264884	30.403017465485643	33.13972253470342	19.324662486546053
30	16.905720409081027	33.79537035661569	31.70499494253122	17.59391429177206
31	21.70349593309286	31.555229044590803	29.4471626182158	17.294112404100535
32	20.049993990457658	30.552783363426062	32.13741189943402	17.259810746682263
33	20.509555172325314	31.42950131602225	30.255817439556832	17.8051260720956
34	17.2411744131243	30.94279590918974	32.05854509655106	19.7574845811349
35	19.415548374114945	28.676995877048817	33.39644478175908	18.51101096707716
36	21.42759716889785	31.16035484659462	29.91415132432757	17.49789666017996
37	17.058052179033044	34.21549813704187	31.772247798414295	16.95420188551079
38	16.938806653441173	33.03074049715796	30.905793333864512	19.124659515536354
39	19.382056992068755	31.251645871849547	31.782241194670007	17.584055941411687
40	19.363285612615446	30.495658949694054	31.783456607728137	18.357598829962363
41	16.77688662491948	31.234630089035758	31.688114205612777	20.300369080431985
42	16.46574088203876	30.534957305240184	31.549827208776904	21.44947460394415
43	19.33857221376685	29.269442219938448	30.602480252913956	20.78950531338075
44	16.797413601012305	32.670167956580045	30.754001747493888	19.778416694913766
45	16.268438828936013	34.64710481857259	30.090656309546798	18.993800042944596
46	17.912082421210847	35.6130881079935	28.518722087701505	17.956107383094142
47	18.732486235447116	30.469325000101282	31.28486716210504	19.513321602346558
48	18.004858951314603	30.345082776381556	34.07383499282231	17.576223279481532
49	18.050909601628113	31.912155345994336	31.715528522368324	18.321406530009224
50	16.606728796781585	32.76618558817214	32.014114996981725	18.61297061806455
51	18.095339701197453	30.881350026806608	30.664871456564512	20.358438815431423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	604.0
1	886.5
2	1169.0
3	2820.5
4	4472.0
5	4145.5
6	3819.0
7	3172.5
8	2526.0
9	2664.5
10	2803.0
11	2661.0
12	2519.0
13	2469.5
14	2420.0
15	2651.0
16	2882.0
17	3173.5
18	3465.0
19	4066.5
20	4668.0
21	5863.0
22	7058.0
23	8168.5
24	9279.0
25	12227.0
26	19418.0
27	23661.0
28	29889.0
29	36117.0
30	39478.5
31	42840.0
32	45939.5
33	49039.0
34	50274.0
35	51509.0
36	55320.0
37	59131.0
38	62347.5
39	65564.0
40	66712.5
41	67861.0
42	66247.5
43	64634.0
44	61071.0
45	57508.0
46	53400.0
47	49292.0
48	41554.5
49	33817.0
50	27892.0
51	21967.0
52	17811.5
53	13656.0
54	11950.0
55	10244.0
56	9610.0
57	8976.0
58	8505.0
59	8034.0
60	6815.5
61	5597.0
62	4438.5
63	3280.0
64	2625.0
65	1970.0
66	1556.0
67	1142.0
68	964.5
69	787.0
70	607.5
71	428.0
72	338.0
73	248.0
74	188.5
75	105.0
76	81.0
77	71.0
78	61.0
79	42.0
80	23.0
81	17.0
82	11.0
83	10.0
84	9.0
85	8.5
86	8.0
87	6.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	740489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.84992870646419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46999349866711	16.78784113508931
2	8.396460836164358	3.8371706298594863
3	3.659952425911482	2.5088895600338414
4	2.162077060929768	1.9761322680050732
5	1.500429856348099	1.7142357623302185
6	1.138336196906816	1.5606540565984997
7	0.9171526178481493	1.4669810351544048
8	0.733356793878709	1.3405720365223723
9	0.6316311823644084	1.2989454737225772
>10	5.417221278099349	25.24972255172316
>50	1.359933523653107	22.73328662062918
>100	0.6069222974556661	17.91567152317141
>500	0.0029692871695482684	0.4644874089118652
>1k	0.0035631446034579224	1.145409938248628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1747	0.23592517917214165	No Hit
AAGGAAAGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1577	0.21296737696306092	No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1523	0.20567489861429405	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1331	0.17974608670756756	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	1224	0.16529617590538145	No Hit
GAATCTATCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	1039	0.14031268526608767	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	784	0.10587598195246654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0025658720116031432	0.0
2	0.0	0.0	0.0	0.015125140278923793	0.0
3	0.0	0.0	0.0	0.02133725146491035	0.0
4	0.0	0.0	0.0	0.03254606077875566	0.0
5	0.0	0.0	0.0	0.07724625213878937	0.0
6	0.0	0.0	0.0	0.09358680547584096	0.0
7	0.0	0.0	0.0	0.11613946999887911	0.0
8	0.0	0.0	0.0	0.3681351107173773	0.0
9	0.0	0.0	0.0	0.434847783019059	0.0
10	0.0	0.0	0.0	0.7870474780854273	0.0
11	0.0	0.0	0.0	0.9526137457815038	0.0
12	0.0	0.0	0.0	1.224866270802132	0.0
13	0.0	0.0	0.0	1.2994116050339708	0.0
14	0.0	0.0	0.0	1.3291217020104282	0.0
15	0.0	0.0	0.0	1.385705932161045	0.0
16	0.0	0.0	0.0	1.4794277835322334	0.0
17	0.0	0.0	0.0	1.591110738984644	0.0
18	0.0	0.0	0.0	1.7239959000066172	0.0
19	0.0	0.0	0.0	1.8058337125872228	0.0
20	0.0	0.0	0.0	1.9113045568536466	0.0
21	0.0	0.0	0.0	2.0379776066896333	0.0
22	0.0	0.0	0.0	2.1859879079905307	0.0
23	0.0	0.0	0.0	2.3383196779425486	0.0
24	0.0	0.0	0.0	2.449732541604264	0.0
25	0.0	0.0	0.0	2.5518272384869998	0.0
26	0.0	0.0	0.0	2.6590536793929416	0.0
27	0.0	0.0	0.0	2.760068009112897	0.0
28	0.0	0.0	0.0	2.889847114541877	0.0
29	0.0	0.0	0.0	3.0120636498313953	0.0
30	0.0	0.0	0.0	3.167501475376407	0.0
31	0.0	0.0	0.0	3.3132159964563956	0.0
32	0.0	0.0	0.0	3.455014186571306	0.0
33	0.0	0.0	0.0	3.5843881543142437	0.0
34	0.0	0.0	0.0	3.7237555183128985	0.0
35	0.0	0.0	0.0	3.892157749811273	0.0
36	0.0	0.0	0.0	4.043139060809816	0.0
37	0.0	0.0	0.0	4.21046092514541	0.0
38	0.0	0.0	0.0	4.384264992457687	0.0
39	0.0	0.0	0.0	4.5752198884791	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0316583E-4	45.000004	36
AGCGTAA	30	2.1644519E-6	45.000004	1
AACCGCG	30	2.1644519E-6	45.000004	25
CGTATAG	60	0.0	45.000004	2
CTGTACG	30	2.1644519E-6	45.000004	25
CGACGAA	20	7.0316583E-4	45.000004	17
GGGTCGA	20	7.0316583E-4	45.000004	11
GCGCGAG	20	7.0316583E-4	45.000004	2
ACCGCGT	30	2.1644519E-6	45.000004	26
ATTACGC	30	2.1644519E-6	45.000004	29
CGCGAAG	20	7.0316583E-4	45.000004	2
CTCGCTA	20	7.0316583E-4	45.000004	1
TCGTATA	30	2.1644519E-6	45.000004	1
CCGAATA	40	6.8102963E-9	45.000004	1
ATTGCGG	20	7.0316583E-4	45.000004	2
TATACGC	20	7.0316583E-4	45.000004	13
TAAGCGC	30	2.1644519E-6	45.000004	19
ACCCCGA	30	2.1644519E-6	45.000004	24
TATCGTC	20	7.0316583E-4	45.000004	25
GTGCGAA	20	7.0316583E-4	45.000004	1
>>END_MODULE