SRR3549391_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1436414
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23323	1.623696232423243	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	4872	0.33917798072143546	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	3184	0.22166311383765405	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2364	0.16457650788700195	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	2198	0.15301995107260163	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	2028	0.14118492300966157	Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1878	0.13074225118942032	Illumina PCR Primer Index 10 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1729	0.12036919718131402	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	1705	0.11869836969007543	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1552	0.10804684443342935	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1437	0.10004079603791106	Illumina PCR Primer Index 10 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCCGG	35	1.2122109E-7	45.000004	2
GAGTACG	35	1.2122109E-7	45.000004	1
GCCATCG	35	1.2122109E-7	45.000004	23
TTGAGCG	35	1.2122109E-7	45.000004	1
CCTCCGC	35	1.2122109E-7	45.000004	45
CGGTCTA	75	0.0	45.000004	31
ATACGAG	35	1.2122109E-7	45.000004	1
ATACCCG	35	1.2122109E-7	45.000004	30
TCGCGCA	35	1.2122109E-7	45.000004	34
CGGACAA	20	7.0338754E-4	45.0	6
CGGGTCA	20	7.0338754E-4	45.0	6
CGAACAT	20	7.0338754E-4	45.0	13
CGTATCG	25	3.891294E-5	45.0	14
GGTCGAA	25	3.891294E-5	45.0	9
CGCGGGC	40	6.8175723E-9	45.0	4
GACCGAC	20	7.0338754E-4	45.0	9
GCCGGTC	20	7.0338754E-4	45.0	28
CGTCTAC	55	1.8189894E-12	45.0	11
TACGGGT	45	3.8562575E-10	45.0	4
CGACACT	20	7.0338754E-4	45.0	18