##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1436414
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.366457720406515	33.0	31.0	34.0	31.0	34.0
2	32.70146907507167	34.0	31.0	34.0	31.0	34.0
3	32.68253790341782	34.0	31.0	34.0	31.0	34.0
4	36.23770862717851	37.0	37.0	37.0	35.0	37.0
5	36.27399482321949	37.0	37.0	37.0	35.0	37.0
6	36.296264865143335	37.0	37.0	37.0	35.0	37.0
7	36.39012011857306	37.0	37.0	37.0	35.0	37.0
8	36.21834791362379	37.0	37.0	37.0	35.0	37.0
9	38.14115498735045	39.0	39.0	39.0	37.0	39.0
10	37.87771631298497	39.0	38.0	39.0	35.0	39.0
11	37.79052974977966	39.0	38.0	39.0	35.0	39.0
12	37.729948329659834	39.0	37.0	39.0	35.0	39.0
13	37.78410124100712	39.0	38.0	39.0	35.0	39.0
14	39.1646287212461	41.0	39.0	41.0	35.0	41.0
15	39.16570710115607	41.0	39.0	41.0	35.0	41.0
16	39.172212189521964	41.0	39.0	41.0	35.0	41.0
17	39.079793151556586	41.0	38.0	41.0	35.0	41.0
18	38.97348953713902	40.0	38.0	41.0	35.0	41.0
19	38.92366824606277	40.0	38.0	41.0	35.0	41.0
20	38.88849663119407	40.0	38.0	41.0	35.0	41.0
21	38.866794670617246	40.0	38.0	41.0	35.0	41.0
22	38.8509357330129	40.0	38.0	41.0	35.0	41.0
23	38.845673322593626	40.0	38.0	41.0	35.0	41.0
24	38.79307010374446	40.0	38.0	41.0	35.0	41.0
25	38.70174476160773	40.0	38.0	41.0	35.0	41.0
26	38.67993698195646	40.0	38.0	41.0	35.0	41.0
27	38.67861981295086	40.0	38.0	41.0	35.0	41.0
28	38.54208744832618	40.0	37.0	41.0	35.0	41.0
29	38.51137137343412	40.0	37.0	41.0	35.0	41.0
30	38.361299040527314	40.0	37.0	41.0	35.0	41.0
31	38.271754522024985	40.0	37.0	41.0	35.0	41.0
32	38.08858936211983	40.0	37.0	41.0	34.0	41.0
33	37.89724480546695	40.0	37.0	41.0	34.0	41.0
34	37.660203813106804	40.0	37.0	41.0	34.0	41.0
35	37.521092108542526	40.0	37.0	41.0	34.0	41.0
36	37.438123688574464	40.0	37.0	41.0	33.0	41.0
37	37.36461632927554	40.0	36.0	41.0	33.0	41.0
38	37.28818432568883	40.0	36.0	41.0	33.0	41.0
39	37.19518258663589	40.0	36.0	41.0	33.0	41.0
40	37.064898420650316	40.0	36.0	41.0	33.0	41.0
41	36.917195878068576	40.0	36.0	40.0	33.0	41.0
42	36.887666786873424	40.0	36.0	40.0	33.0	41.0
43	36.79307358463507	40.0	35.0	40.0	32.0	41.0
44	36.68190507750551	39.0	35.0	40.0	32.0	41.0
45	36.6188751989329	39.0	35.0	40.0	32.0	41.0
46	36.521891321025834	39.0	35.0	40.0	31.0	41.0
47	36.44482092210185	39.0	35.0	40.0	31.0	41.0
48	36.344527413405885	39.0	35.0	40.0	31.0	41.0
49	36.249560363516366	39.0	35.0	40.0	31.0	41.0
50	36.17493006890771	38.0	35.0	40.0	31.0	41.0
51	36.13795952977345	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	7.0
14	9.0
15	22.0
16	33.0
17	66.0
18	177.0
19	367.0
20	647.0
21	1085.0
22	1727.0
23	2732.0
24	4435.0
25	7934.0
26	12743.0
27	16439.0
28	16926.0
29	15512.0
30	15519.0
31	17527.0
32	21500.0
33	28224.0
34	52613.0
35	108758.0
36	75278.0
37	106555.0
38	265847.0
39	663722.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.936235653509367	24.79452302748372	31.78589181113523	15.483349507871685
2	29.860820069979827	26.93109368190508	30.68885432751282	12.519231920602278
3	26.320475851669507	25.776273414210664	35.614732242932746	12.28851849118708
4	25.36636373636013	26.1542981341034	35.36007028614313	13.11926784339334
5	24.012297290335518	31.021975558578518	31.598271807431562	13.367455343654406
6	19.978641255237	40.60368389614693	28.67084280715727	10.7468320414588
7	84.93811672679325	3.2430761604941196	9.986187826072427	1.8326192866402025
8	84.78767263476963	3.752469691885487	9.117636001876896	2.3422216714679753
9	79.1646419486304	6.765319747649355	10.574736809861223	3.495301493859013
10	31.02796269042212	46.155982885157066	15.35379075948856	7.462263664932254
11	22.777625392122328	24.827661106059953	36.67403687237802	15.720676629439703
12	24.95610596944892	23.25283657775544	34.99095664620367	16.80010080659197
13	21.174675267715294	26.999458373421593	34.688328016853085	17.137538342010032
14	15.140899489979908	30.10295082058515	35.18616499143005	19.56998469800489
15	13.844198121154486	31.010975944261194	39.05726343519348	16.087562499390845
16	17.53547375617336	29.621752503108432	36.35114945969616	16.491624281022048
17	17.193232591717987	30.223320017766465	32.211256643279725	20.372190747235823
18	16.195191636951463	30.669500575739306	36.82907574000253	16.306232047306697
19	19.077926001835124	28.297204009429038	34.71241577985177	17.912454208884068
20	20.583063100192565	29.03703249898706	35.7728342942912	14.607070106529177
21	19.692024722677445	29.334370174615398	36.029654403257	14.94395069945016
22	18.688832049812937	25.142124763473483	36.09412049729396	20.074922689419626
23	15.535214777912218	29.69276267148608	36.59028664437968	18.18173590622202
24	15.934890637378917	27.903097574933135	39.87610814152466	16.285903646163295
25	15.224719335790379	33.00559587973941	34.64690541863279	17.122779365837427
26	15.024985832775231	33.633548545196575	32.93173138106423	18.409734240963957
27	15.65614091759061	34.14043583535109	32.75302245731384	17.45040078974446
28	13.540385988997599	31.50205999106107	38.11220163546164	16.845352384479682
29	14.91679975271753	27.25077867522873	36.66610044179463	21.166321130259103
30	16.003603417956107	31.718641004612874	34.80076078345101	17.47699479398001
31	19.378883803694478	30.41671829987733	32.72802966275739	17.476368233670794
32	18.967024827104165	29.226462565806237	34.1986363262959	17.6078762807937
33	17.252825438905496	30.383092896616155	33.551469144689484	18.812612519788864
34	16.364571773875777	29.85650376562746	33.89092559665946	19.887998863837307
35	17.217041883468138	28.223269892941726	35.172798371500136	19.386889852089997
36	20.10332675677068	29.31626954346031	32.50406916111929	18.07633453864972
37	15.917764655593722	32.81205836200427	34.22536956615572	17.04480741624629
38	15.9918380077053	32.07807776866558	33.667730890954836	18.262353332674287
39	16.819176087116947	31.93953832251705	33.01207033626795	18.22921525409805
40	19.00949169250648	29.912685340020357	33.350552138868046	17.727270828605125
41	15.687677786487738	29.117789161063595	34.10674081427778	21.087792238170888
42	16.87333874495793	28.89438560192257	32.746617618597426	21.485658034522082
43	17.37841597199693	28.357493034737896	33.499673492461085	20.764417500804086
44	15.928276945226097	31.09535273256874	32.904232345270934	20.072137976934226
45	15.465875437025817	33.03650618832732	31.914545527960602	19.58307284668626
46	17.609129401412126	32.152081502965025	31.391158816330112	18.847630279292737
47	16.078442566001165	29.918533236239693	34.06385624200266	19.93916795575649
48	17.02886493726739	28.293375029761613	35.25292847326746	19.42483155970354
49	16.591247370187144	29.60406957882616	34.44570994156281	19.358973109423886
50	16.193242338211686	30.439413706633324	34.32729004312127	19.040053912033716
51	16.705350964276317	29.390203659947616	31.703255468131054	22.20118990764501
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1105.0
1	1756.0
2	2407.0
3	17579.0
4	32751.0
5	20763.5
6	8776.0
7	8446.0
8	8116.0
9	8545.0
10	8974.0
11	9518.0
12	10062.0
13	10047.0
14	10032.0
15	10181.0
16	10330.0
17	9774.0
18	9218.0
19	9206.5
20	9195.0
21	10185.5
22	11176.0
23	12713.5
24	14251.0
25	18605.0
26	28505.0
27	34051.0
28	38775.0
29	43499.0
30	52366.0
31	61233.0
32	68310.5
33	75388.0
34	82703.5
35	90019.0
36	93834.5
37	97650.0
38	100178.5
39	102707.0
40	112801.5
41	122896.0
42	124164.5
43	125433.0
44	127974.0
45	130515.0
46	130199.5
47	129884.0
48	114547.5
49	99211.0
50	84434.0
51	69657.0
52	54123.0
53	38589.0
54	29845.0
55	21101.0
56	16565.0
57	12029.0
58	10121.5
59	8214.0
60	7166.5
61	6119.0
62	4980.0
63	3841.0
64	3067.5
65	2294.0
66	1718.5
67	1143.0
68	910.5
69	678.0
70	587.0
71	496.0
72	365.0
73	234.0
74	178.5
75	76.5
76	30.0
77	25.5
78	21.0
79	11.5
80	2.0
81	2.0
82	2.0
83	1.5
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1436414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.529813426432977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4263901752261	15.074300908738634
2	10.57059464153037	4.340246715941413
3	4.140383176207492	2.5500388236444533
4	2.239544118844905	1.8390969168060454
5	1.374301383152556	1.4107075493905377
6	0.9124557573977932	1.123952787555077
7	0.6572729371004954	0.9445583538241752
8	0.5188477805142372	0.852147850454091
9	0.38973976632511786	0.7201156218764629
>10	3.7861599142653475	16.746661894326405
>50	0.8997269025516597	13.332961361708934
>100	1.0729872716656137	36.64829232898644
>500	0.007162343517156942	1.021308775734314
>1k	0.004092767724089681	1.762538358891002
>5k	0.0	0.0
>10k+	3.410639770074734E-4	1.633071752121994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23323	1.623696232423243	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	4872	0.33917798072143546	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	3184	0.22166311383765405	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2364	0.16457650788700195	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	2198	0.15301995107260163	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	2028	0.14118492300966157	Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1878	0.13074225118942032	Illumina PCR Primer Index 10 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1729	0.12036919718131402	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	1705	0.11869836969007543	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1552	0.10804684443342935	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1437	0.10004079603791106	Illumina PCR Primer Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.96178121349416E-5	0.0	0.0	0.15100103452068833	0.0
2	6.96178121349416E-5	0.0	0.0	0.6421546991327013	0.0
3	6.96178121349416E-5	0.0	0.0	0.8879751937811801	0.0
4	6.96178121349416E-5	0.0	0.0	1.2371085216379123	0.0
5	6.96178121349416E-5	0.0	0.0	2.2165615205644054	0.0
6	6.96178121349416E-5	0.0	0.0	2.7945982147208257	0.0
7	6.96178121349416E-5	0.0	0.0	3.3051056311063522	0.0
8	6.96178121349416E-5	0.0	0.0	4.141076319222731	0.0
9	6.96178121349416E-5	0.0	0.0	4.3828589807673834	0.0
10	6.96178121349416E-5	0.0	0.0	5.216741134519713	0.0
11	6.96178121349416E-5	0.0	0.0	6.080419711865799	0.0
12	6.96178121349416E-5	0.0	0.0	6.873575445519188	0.0
13	6.96178121349416E-5	0.0	0.0	7.111598745208554	0.0
14	6.96178121349416E-5	0.0	0.0	7.203772728475217	0.0
15	6.96178121349416E-5	0.0	0.0	7.376216049133467	0.0
16	6.96178121349416E-5	0.0	0.0	7.691584738104752	0.0
17	6.96178121349416E-5	0.0	0.0	8.115557214006547	0.0
18	2.088534364048248E-4	0.0	0.0	8.5780979578311	0.0
19	2.088534364048248E-4	0.0	0.0	8.92792746380918	0.0
20	2.088534364048248E-4	0.0	0.0	9.239884879985853	0.0
21	2.088534364048248E-4	0.0	0.0	9.618466542375666	0.0
22	2.088534364048248E-4	0.0	0.0	10.124170329723881	0.0
23	2.088534364048248E-4	0.0	0.0	10.559977833688617	0.0
24	2.088534364048248E-4	0.0	0.0	10.904516385944442	0.0
25	2.088534364048248E-4	0.0	0.0	11.200183234081539	0.0
26	2.088534364048248E-4	0.0	0.0	11.482065755415917	0.0
27	2.088534364048248E-4	0.0	0.0	11.768682287975473	0.0
28	2.784712485397664E-4	0.0	0.0	12.060380920820878	0.0
29	2.784712485397664E-4	0.0	0.0	12.399698137166583	0.0
30	2.784712485397664E-4	0.0	0.0	12.781830307975277	0.0
31	2.784712485397664E-4	0.0	0.0	13.11690083778075	0.0
32	2.784712485397664E-4	0.0	0.0	13.429693667703043	0.0
33	2.784712485397664E-4	0.0	0.0	13.729537584568238	0.0
34	2.784712485397664E-4	0.0	0.0	14.053956589117066	0.0
35	2.784712485397664E-4	0.0	0.0	14.40650118976841	0.0
36	2.784712485397664E-4	0.0	0.0	14.738369300215677	0.0
37	3.48089060674708E-4	0.0	0.0	15.051788690447182	0.0
38	3.48089060674708E-4	0.0	0.0	15.391175524605023	0.0
39	3.48089060674708E-4	0.0	0.0	15.741910062140859	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCCGG	35	1.2122109E-7	45.000004	2
GAGTACG	35	1.2122109E-7	45.000004	1
GCCATCG	35	1.2122109E-7	45.000004	23
TTGAGCG	35	1.2122109E-7	45.000004	1
CCTCCGC	35	1.2122109E-7	45.000004	45
CGGTCTA	75	0.0	45.000004	31
ATACGAG	35	1.2122109E-7	45.000004	1
ATACCCG	35	1.2122109E-7	45.000004	30
TCGCGCA	35	1.2122109E-7	45.000004	34
CGGACAA	20	7.0338754E-4	45.0	6
CGGGTCA	20	7.0338754E-4	45.0	6
CGAACAT	20	7.0338754E-4	45.0	13
CGTATCG	25	3.891294E-5	45.0	14
GGTCGAA	25	3.891294E-5	45.0	9
CGCGGGC	40	6.8175723E-9	45.0	4
GACCGAC	20	7.0338754E-4	45.0	9
GCCGGTC	20	7.0338754E-4	45.0	28
CGTCTAC	55	1.8189894E-12	45.0	11
TACGGGT	45	3.8562575E-10	45.0	4
CGACACT	20	7.0338754E-4	45.0	18
>>END_MODULE