##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	982186
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.783436131241945	31.0	31.0	34.0	30.0	34.0
2	32.04409551754963	33.0	31.0	34.0	30.0	34.0
3	31.852061625802037	33.0	31.0	34.0	30.0	34.0
4	35.685403782990186	37.0	35.0	37.0	33.0	37.0
5	28.379062621540115	35.0	32.0	37.0	0.0	37.0
6	31.95567641974127	37.0	32.0	37.0	17.0	37.0
7	34.950725219052195	37.0	33.0	37.0	32.0	37.0
8	35.609167713650976	37.0	35.0	37.0	35.0	37.0
9	37.661903142581956	39.0	37.0	39.0	35.0	39.0
10	37.24509512454871	39.0	37.0	39.0	34.0	39.0
11	37.10837254858041	39.0	37.0	39.0	33.0	39.0
12	37.02236439941111	39.0	37.0	39.0	33.0	39.0
13	36.70696589037107	39.0	35.0	39.0	33.0	39.0
14	38.118203680361965	40.0	37.0	41.0	33.0	41.0
15	38.28981883268546	40.0	37.0	41.0	33.0	41.0
16	38.28650479644385	40.0	37.0	41.0	34.0	41.0
17	38.24868914849122	40.0	37.0	41.0	34.0	41.0
18	38.07787425192377	40.0	37.0	41.0	33.0	41.0
19	38.11893368465851	40.0	37.0	41.0	34.0	41.0
20	38.13385244749976	40.0	36.0	41.0	34.0	41.0
21	38.083300922635836	40.0	36.0	41.0	34.0	41.0
22	37.90317007165649	40.0	36.0	41.0	33.0	41.0
23	37.90630695204371	40.0	36.0	41.0	33.0	41.0
24	37.858735514454494	40.0	36.0	41.0	33.0	41.0
25	37.749551510610004	40.0	36.0	41.0	33.0	41.0
26	37.659321146911076	40.0	36.0	41.0	33.0	41.0
27	37.56019328314596	40.0	35.0	41.0	33.0	41.0
28	37.53337046139937	40.0	36.0	41.0	33.0	41.0
29	37.482451389044435	39.0	36.0	41.0	33.0	41.0
30	37.28041430034637	39.0	35.0	41.0	33.0	41.0
31	37.21176844304439	39.0	35.0	40.0	32.0	41.0
32	37.04994064260741	39.0	35.0	40.0	32.0	41.0
33	36.900939333283105	39.0	35.0	40.0	31.0	41.0
34	36.672021389024074	39.0	35.0	40.0	31.0	41.0
35	36.498093029222574	39.0	35.0	40.0	30.0	41.0
36	36.41560865253628	39.0	35.0	40.0	30.0	41.0
37	36.28877218775263	39.0	35.0	40.0	30.0	41.0
38	36.158312173050724	38.0	35.0	40.0	30.0	41.0
39	36.033372497673554	38.0	35.0	40.0	30.0	41.0
40	35.87337632586903	38.0	35.0	40.0	29.0	41.0
41	35.73947093524037	38.0	35.0	40.0	29.0	41.0
42	35.689810280333866	38.0	35.0	40.0	29.0	41.0
43	35.549866318599534	38.0	35.0	40.0	28.0	41.0
44	35.35565361347036	38.0	35.0	40.0	27.0	41.0
45	35.27543153740738	38.0	34.0	40.0	27.0	41.0
46	35.14631953621819	38.0	34.0	40.0	27.0	41.0
47	35.00865212902648	38.0	34.0	40.0	26.0	41.0
48	34.91700757290371	38.0	34.0	40.0	26.0	40.0
49	34.85500506014136	38.0	34.0	40.0	26.0	40.0
50	34.74993127574614	38.0	34.0	40.0	26.0	40.0
51	35.0236462340127	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	3.0
13	2.0
14	4.0
15	13.0
16	31.0
17	72.0
18	212.0
19	447.0
20	835.0
21	1335.0
22	2101.0
23	3482.0
24	5417.0
25	8349.0
26	11714.0
27	13925.0
28	15234.0
29	16628.0
30	19479.0
31	23977.0
32	30894.0
33	43690.0
34	62021.0
35	85956.0
36	86958.0
37	142475.0
38	200953.0
39	205975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.097006066060807	25.20734361923302	31.668645246419718	16.027005068286453
2	28.2766197033963	27.12907738452798	31.737064059149695	12.857238852926024
3	26.863445416652244	25.780656616974788	34.91904791964048	12.436850046732491
4	25.567458709450147	27.110445475704193	33.94866145516226	13.3734343596834
5	18.72099581952909	45.99403778917639	24.60236655786175	10.682599833432771
6	20.333724976735567	41.02746322997884	28.205451920511997	10.433359872773588
7	84.95692261954456	4.257340259380606	8.639911381347321	2.145825739727506
8	85.16380807708519	4.288087999625326	8.113025435100887	2.4350784881885916
9	79.60966660082714	7.892293313079193	9.288566524059599	3.209473562034075
10	30.921128991861014	47.918622338335105	13.890953444663232	7.269295225140655
11	23.55704520325071	25.599020959370222	35.54428590918624	15.299647928192828
12	25.80315744675652	24.50513446536603	33.52929078606293	16.162417301814525
13	21.326714084704935	28.31724337345472	32.83084873944446	17.52519380239588
14	15.455117462476558	31.03699299318052	34.057398496822394	19.45049104752053
15	14.526881873698057	31.873087174934277	37.5703787266363	16.029652224731365
16	18.286658535145076	30.352499424752544	35.30858717187987	16.052254868222516
17	17.94792432390606	30.755987155182417	31.14644273080659	20.149645790104927
18	16.7752340188111	31.81413703718033	35.67918907416722	15.731439869841354
19	20.082346928178573	28.669926062884223	33.70359585658928	17.544131152347926
20	21.326612270995515	29.522615879273378	34.76011671923648	14.39065513049463
21	20.303486305037946	29.879371117079657	34.893085423738476	14.924057154143918
22	19.573889263337087	25.85946042806556	35.10913411512687	19.457516193470482
23	16.155493969573993	30.43639392131429	35.70504975635979	17.70306235275192
24	16.100412752777988	28.71492772244768	39.629968254485405	15.554691270288926
25	15.656912234546205	33.92351346893562	34.194439749701175	16.225134546816996
26	15.531477744541258	35.04967490882582	31.71201788663247	17.70682946000045
27	15.756995110905676	35.661575302437626	31.801206696084037	16.78022289057266
28	14.098653411879218	32.50504486930174	37.525987949329355	15.87031376948969
29	15.309320230587689	28.661170083874133	36.01649789347435	20.013011792063825
30	16.33152987316048	32.970129893930476	34.07725217015922	16.621088062749827
31	20.36131649198828	30.796712638950257	32.4596359549006	16.38233491416086
32	19.755524920941657	29.774604810086885	33.69504350499803	16.774826763973422
33	17.86647335637038	31.516841005675094	33.014011602690324	17.602674035264197
34	16.9591095780229	30.468974308328566	33.577550484327816	18.99436562932072
35	17.82890409759455	29.004689539455867	35.237012134157894	17.929394228791693
36	21.039395796722822	30.15365725025606	32.15714742421497	16.649799528806152
37	16.506547639652773	33.65441983493962	33.61909047777101	16.2199420476366
38	16.455640784942975	33.149322022508976	32.70633057282429	17.68870661972376
39	17.18880130647352	33.249812153706124	32.0716239083025	17.48976263151786
40	19.28290568181587	31.081892839034563	32.29072701097348	17.344474468176088
41	15.682467475610526	30.02333570219897	34.273854443048464	20.020342379142036
42	16.928870906325276	30.000834872417244	32.20113094668423	20.86916327457325
43	17.620491434412628	29.617608070161864	32.91871397067358	19.84318652475193
44	16.038000948903772	32.49435442981268	32.5157353087908	18.951909312492745
45	15.63033885638769	34.94898115020984	31.172914295255683	18.247765698146786
46	17.48395925008094	33.86273068441212	30.540956600888226	18.112353464618717
47	16.62658600305848	31.34559034643133	32.904867306192514	19.122956344317675
48	17.471436163822332	29.539822396165288	34.73883765396778	18.249903786044598
49	16.551142044378558	31.475606453360154	33.46494452170974	18.508306980551545
50	16.45075372689083	32.713253905064825	33.23087480375408	17.605117564290268
51	17.162126114605584	31.24540565636244	31.21822139594741	20.37424683308457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	707.0
1	1073.0
2	1439.0
3	9623.0
4	17807.0
5	11973.5
6	6140.0
7	5688.5
8	5237.0
9	5575.0
10	5913.0
11	6307.0
12	6701.0
13	6678.5
14	6656.0
15	6724.0
16	6792.0
17	6548.0
18	6304.0
19	6348.5
20	6393.0
21	7118.0
22	7843.0
23	9165.0
24	10487.0
25	13676.0
26	21128.0
27	25391.0
28	28571.5
29	31752.0
30	38898.0
31	46044.0
32	51682.0
33	57320.0
34	61984.5
35	66649.0
36	69341.0
37	72033.0
38	74464.0
39	76895.0
40	83596.5
41	90298.0
42	90657.5
43	91017.0
44	91088.5
45	91160.0
46	84012.5
47	76865.0
48	66045.5
49	55226.0
50	46971.5
51	38717.0
52	30790.5
53	22864.0
54	17600.0
55	12336.0
56	9875.5
57	7415.0
58	6335.0
59	5255.0
60	4679.5
61	4104.0
62	3251.5
63	2399.0
64	1913.0
65	1427.0
66	1094.5
67	762.0
68	611.0
69	460.0
70	366.0
71	272.0
72	202.5
73	133.0
74	97.5
75	42.5
76	23.0
77	18.5
78	14.0
79	8.5
80	3.0
81	2.5
82	2.0
83	1.5
84	1.0
85	0.5
86	0.0
87	1.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	982186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.60931936156472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23299332813428	17.52590446648825
2	8.803278188482029	4.156788123611383
3	3.8096830711783913	2.6983207288139197
4	2.175320577839736	2.054313529440073
5	1.450467938328831	1.7122280389857865
6	1.055957312704413	1.4958260056690886
7	0.781882007324264	1.2921791409785883
8	0.6081516017567437	1.1486436308897667
9	0.5040419598773609	1.0710078842136244
>10	4.8287770963131695	24.404635676492532
>50	1.0729355754171273	18.332010046594817
>100	0.6699262225011837	21.746757615384148
>500	0.003951072085524675	0.5534739279471126
>1k	0.0021950400475137083	0.8175659819563339
>5k	4.390080095027417E-4	0.9903452025345806
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9555	0.9728299935042853	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2444	0.2488327058215043	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	1578	0.16066203346413002	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	1342	0.13663399804110424	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1305	0.132866890792579	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1219	0.12411091178249334	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.126959659372054E-4	0.0	0.0	0.07330587078211255	0.0
2	7.126959659372054E-4	0.0	0.0	0.2870128468538546	0.0
3	7.126959659372054E-4	0.0	0.0	0.37681253856194247	0.0
4	7.126959659372054E-4	0.0	0.0	0.5195553591682227	0.0
5	7.126959659372054E-4	0.0	0.0	0.9774116104281674	0.0
6	7.126959659372054E-4	0.0	0.0	1.2481342637748858	0.0
7	7.126959659372054E-4	0.0	0.0	1.5119335848810713	0.0
8	7.126959659372054E-4	0.0	0.0	1.9415874386317866	0.0
9	7.126959659372054E-4	0.0	0.0	2.06569835041428	0.0
10	7.126959659372054E-4	0.0	0.0	2.51714033798079	0.0
11	7.126959659372054E-4	0.0	0.0	2.904236061194112	0.0
12	7.126959659372054E-4	0.0	0.0	3.3065020271109544	0.0
13	7.126959659372054E-4	0.0	0.0	3.4266422042260833	0.0
14	8.145096753568061E-4	0.0	0.0	3.4778545000641428	0.0
15	0.0010181370941960077	0.0	0.0	3.5633780159766073	0.0
16	0.0011199508036156085	0.0	0.0	3.729741617168235	0.0
17	0.0011199508036156085	0.0	0.0	3.947622955326181	0.0
18	0.0011199508036156085	0.0	0.0	4.206637032089645	0.0
19	0.0011199508036156085	0.0	0.0	4.378396759880512	0.0
20	0.0011199508036156085	0.0	0.0	4.545473057038076	0.0
21	0.0011199508036156085	0.0	0.0	4.767325129863386	0.0
22	0.00132357822245481	0.0	0.0	5.054948858973758	0.0
23	0.00132357822245481	0.0	0.0	5.3168137196009715	0.0
24	0.00132357822245481	0.0	0.0	5.520033883602495	0.0
25	0.00132357822245481	0.0	0.0	5.68690655334122	0.0
26	0.00132357822245481	0.0	0.0	5.840441627145978	0.0
27	0.00132357822245481	0.0	0.0	6.004259885602116	0.0
28	0.00132357822245481	0.0	0.0	6.186200984334943	0.0
29	0.00132357822245481	0.0	0.0	6.392170118490795	0.0
30	0.00132357822245481	0.0	0.0	6.637846599320292	0.0
31	0.0014253919318744108	0.0	0.0	6.851655389101453	0.0
32	0.0014253919318744108	0.0	0.0	7.0603734934116344	0.0
33	0.0014253919318744108	0.0	0.0	7.260030177583472	0.0
34	0.0015272056412940116	0.0	0.0	7.474348035911731	0.0
35	0.0015272056412940116	0.0	0.0	7.71697010545864	0.0
36	0.0015272056412940116	0.0	0.0	7.93383330652239	0.0
37	0.0015272056412940116	0.0	0.0	8.15303822290279	0.0
38	0.0016290193507136122	0.0	0.0	8.394642155355504	0.0
39	0.0016290193507136122	0.0	0.0	8.633802558782145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGG	35	1.2116834E-7	45.000004	2
GTCTCGC	35	1.2116834E-7	45.000004	31
TCTCGCG	35	1.2116834E-7	45.000004	32
CTCGCGG	35	1.2116834E-7	45.000004	2
ACCCGAT	35	1.2116834E-7	45.000004	32
CGGAATA	25	3.8903898E-5	45.0	9
CGTGGCA	20	7.032784E-4	45.0	37
CTCGTTG	25	3.8903898E-5	45.0	1
GGTCGAA	20	7.032784E-4	45.0	9
CGACGGT	55	1.8189894E-12	45.0	28
CCCTATA	20	7.032784E-4	45.0	43
GCGCGAG	45	3.8380676E-10	45.0	1
CGGTTGG	25	3.8903898E-5	45.0	10
CGTTGAT	20	7.032784E-4	45.0	25
CCTACGG	20	7.032784E-4	45.0	2
CATTCGT	20	7.032784E-4	45.0	16
GCGTTCC	25	3.8903898E-5	45.0	28
ACGGGCG	25	3.8903898E-5	45.0	5
TATTGCG	25	3.8903898E-5	45.0	1
CCCGTCC	20	7.032784E-4	45.0	13
>>END_MODULE