FastQCFastQC Report
Sat 18 Jun 2016
SRR3549387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394643
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30600.7753843347025032No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.3585519064065497No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG5490.13911307181427265No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC5070.12847054173012062No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC253.8861333E-545.00000429
CAACGGG351.2092096E-745.0000043
GACCCGC351.2092096E-745.00000431
GTTACGG253.8861333E-545.0000042
AGCGGGC351.2092096E-745.0000044
GAGACGG351.2092096E-745.0000042
TACGCGG253.8861333E-545.0000042
CTACGGG351.2092096E-745.0000043
CGGGAGA700.045.0000046
GGACGGC253.8861333E-545.0000048
GCTAGCG253.8861333E-545.0000041
GGGCCGA351.2092096E-745.0000047
TATAGCG502.1827873E-1145.0000041
ACCCGCA351.2092096E-745.00000432
TCACGAC453.8380676E-1045.025
CTCGTAC207.027651E-445.029
CGACGGT406.7957444E-945.028
AGCCAGC207.027651E-445.036
GTCTCGT207.027651E-445.027
TTCACGA207.027651E-445.028