##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394643
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.621886109724485	31.0	31.0	33.0	30.0	34.0
2	31.96926082560694	31.0	31.0	34.0	30.0	34.0
3	31.877377781944695	31.0	31.0	34.0	30.0	34.0
4	35.72357041680709	37.0	35.0	37.0	35.0	37.0
5	35.68378762577824	37.0	35.0	37.0	35.0	37.0
6	35.82180603735528	37.0	35.0	37.0	35.0	37.0
7	22.93734590503316	35.0	0.0	37.0	0.0	37.0
8	28.980275844244037	35.0	17.0	37.0	17.0	37.0
9	35.296541431116225	37.0	32.0	39.0	32.0	39.0
10	36.41665251885882	37.0	35.0	39.0	32.0	39.0
11	36.88803044777178	39.0	37.0	39.0	33.0	39.0
12	37.07730277744696	39.0	37.0	39.0	34.0	39.0
13	37.00155330260514	39.0	37.0	39.0	33.0	39.0
14	38.369085477254124	40.0	38.0	41.0	34.0	41.0
15	38.48099168108899	40.0	38.0	41.0	34.0	41.0
16	38.476384985923985	40.0	38.0	41.0	34.0	41.0
17	38.5364088555986	40.0	38.0	41.0	34.0	41.0
18	38.39259533299717	40.0	38.0	41.0	34.0	41.0
19	38.3936393145197	40.0	37.0	41.0	34.0	41.0
20	38.31623011177191	40.0	37.0	41.0	34.0	41.0
21	38.26542470029875	40.0	37.0	41.0	34.0	41.0
22	38.123177149981125	40.0	37.0	41.0	34.0	41.0
23	38.13560610475797	40.0	37.0	41.0	34.0	41.0
24	38.04081917074419	40.0	36.0	41.0	34.0	41.0
25	37.82586540240167	40.0	36.0	41.0	33.0	41.0
26	37.76248913575054	40.0	36.0	41.0	33.0	41.0
27	37.70756354477338	40.0	36.0	41.0	33.0	41.0
28	37.7216370238418	40.0	36.0	41.0	33.0	41.0
29	37.54080270016192	39.0	36.0	40.0	33.0	41.0
30	37.386997868960044	39.0	36.0	40.0	33.0	41.0
31	37.2465874220498	39.0	36.0	40.0	33.0	41.0
32	37.09727019103342	39.0	35.0	40.0	32.0	41.0
33	36.93875984117291	39.0	35.0	40.0	32.0	41.0
34	36.634289725143994	39.0	35.0	40.0	31.0	41.0
35	36.535537181706	38.0	35.0	40.0	31.0	41.0
36	36.373084027842886	38.0	35.0	40.0	30.0	41.0
37	36.33504205066351	38.0	35.0	40.0	30.0	41.0
38	36.163205732776206	38.0	35.0	40.0	30.0	41.0
39	35.970560734638646	38.0	35.0	40.0	30.0	41.0
40	35.72395050716724	38.0	35.0	40.0	29.0	41.0
41	35.57306476993131	38.0	35.0	40.0	29.0	41.0
42	35.54402079854451	38.0	35.0	40.0	29.0	41.0
43	35.38722592317614	38.0	35.0	40.0	28.0	40.0
44	35.086247570589116	38.0	34.0	40.0	27.0	40.0
45	35.034388041850484	38.0	34.0	40.0	27.0	40.0
46	34.965617026020986	38.0	34.0	40.0	27.0	40.0
47	34.765788320076624	37.0	34.0	39.0	27.0	40.0
48	34.598622045747675	37.0	34.0	39.0	26.0	40.0
49	34.41218519015921	37.0	33.0	39.0	26.0	40.0
50	34.43530228586343	37.0	33.0	39.0	26.0	40.0
51	34.86174339846393	38.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	5.0
16	11.0
17	28.0
18	72.0
19	135.0
20	296.0
21	514.0
22	823.0
23	1303.0
24	2132.0
25	3174.0
26	4429.0
27	5292.0
28	5766.0
29	6550.0
30	7727.0
31	9527.0
32	12601.0
33	17641.0
34	27131.0
35	36678.0
36	45827.0
37	82384.0
38	82628.0
39	41967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.29378197510155	25.338597162498765	31.946087983316566	15.421532879083122
2	28.104134622937693	26.99224362271724	32.09711055308215	12.806511201262913
3	27.06623454615944	25.824859429915136	34.89001451945175	12.218891504473664
4	25.741239550682515	27.240315931107357	33.903553338080236	13.11489118012989
5	23.528353473899195	32.3887665561026	30.767554473283447	13.315325496714753
6	20.310001697736944	41.59683562105498	27.895084924856135	10.198077756351944
7	55.97286661615688	37.61120810454005	5.263998094480328	1.1519271848227386
8	87.58194114680865	3.411437679117582	7.194350336886755	1.812270837187027
9	81.90415134691354	7.130495156381844	8.442820473187158	2.5225330235174575
10	31.194269250943258	48.680199572778434	13.238800637538231	6.886730538740077
11	23.642633975517114	25.56690477216117	35.67553459709155	15.114926655230171
12	26.064062963235124	24.309312467217207	33.439336311552466	16.1872882579952
13	21.395032979173582	28.393003296650388	32.87072113277063	17.341242591405397
14	15.295849666660754	31.08226929148623	34.12197859837879	19.49990244347423
15	14.348157702024361	32.16704717934944	37.640855152631616	15.843939965994583
16	18.42323314996085	30.365672265819992	35.228041546410296	15.983053037808856
17	17.85259082259156	30.952024994742082	30.805056722151413	20.390327460514946
18	16.695849159873607	31.919481658106186	35.83593272907413	15.54873645294608
19	20.160499489411947	28.545546227856565	33.7710284991752	17.52292578355628
20	21.302797718444264	29.560640883025926	34.80208694947079	14.334474449059023
21	20.370562761787237	29.896133974250144	34.973634398684375	14.759668865278238
22	19.667395595512907	25.800787040439083	34.94981540278175	19.582001961266258
23	15.946057576087755	30.639590718700195	35.73381511898096	17.6805365862311
24	16.008392395152075	28.801727130596515	39.81725255484071	15.372627919410709
25	15.59840159333879	34.02518225332769	34.163788537995096	16.21262761533842
26	15.347288562067488	35.310647851349195	31.610848285665778	17.731215300917537
27	15.692664002655565	35.9806204595039	31.59184376765836	16.734871770182163
28	14.01925284370938	32.612766475016656	37.499208145083024	15.868772536190937
29	15.158763743433939	28.542758898548815	36.088059334639155	20.21041802337809
30	16.308156992522356	33.11524593113269	34.14655777500171	16.43003930134324
31	20.4970061549299	31.017147143114155	32.207336757525155	16.278509944430787
32	19.72440914953515	29.918432608712177	33.674738941270974	16.6824193004817
33	17.864246926969436	31.42004292487134	33.08306494730681	17.632645200852416
34	16.838002954569067	30.474124715248973	33.62786113018601	19.060011199995948
35	17.744898553882877	28.919808535815918	35.29645781123699	18.03883509906422
36	21.083865670998854	30.07148232706522	32.21772589403587	16.62692610790005
37	16.279016731577652	33.91495604888469	33.70615974437656	16.099867475161094
38	16.40698048616091	33.210268521169766	32.66369858327653	17.719052409392795
39	17.114201949610152	33.25081149291892	32.089508745879186	17.54547781159174
40	19.281730576749112	31.006758006603434	32.40777107411002	17.30374034253743
41	15.57179526812841	29.96733756838459	34.335082593635256	20.12578456985174
42	16.712826529293565	29.832785580892097	32.43311043145324	21.0212774583611
43	17.477314940338484	29.548984778648048	33.09928213600647	19.874418145007006
44	15.750437737398105	32.5453637844837	32.67206057119979	19.032137906918408
45	15.45751476651049	34.973634398684375	31.147898227005165	18.42095260779996
46	17.327559338440057	33.907861028828584	30.532405237138377	18.232174395592978
47	16.388736148873793	31.43169902924922	32.96827765854202	19.211287163334966
48	17.249514117822944	29.425582108386568	34.99086516167777	18.334038612112717
49	16.41255514477642	31.421816679885367	33.60328195356309	18.562346221775123
50	16.29726106886477	32.7508659725372	33.30199699475222	17.649875963845805
51	17.079993817196808	31.110902765284067	31.271554290840076	20.53754912667905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	401.5
2	551.0
3	3597.0
4	6643.0
5	4491.5
6	2340.0
7	2278.5
8	2217.0
9	2353.0
10	2489.0
11	2558.5
12	2628.0
13	2672.5
14	2717.0
15	2669.5
16	2622.0
17	2563.0
18	2504.0
19	2668.5
20	2833.0
21	3106.5
22	3380.0
23	4068.0
24	4756.0
25	6327.0
26	9452.0
27	11006.0
28	12994.0
29	14982.0
30	17516.0
31	20050.0
32	22207.5
33	24365.0
34	26019.5
35	27674.0
36	28722.0
37	29770.0
38	31258.5
39	32747.0
40	34513.0
41	36279.0
42	36011.0
43	35743.0
44	34775.5
45	33808.0
46	31560.5
47	29313.0
48	24923.5
49	20534.0
50	17301.0
51	14068.0
52	11019.0
53	7970.0
54	6179.0
55	4388.0
56	3546.0
57	2704.0
58	2356.0
59	2008.0
60	1746.0
61	1484.0
62	1158.5
63	833.0
64	650.0
65	467.0
66	367.0
67	267.0
68	218.0
69	169.0
70	132.0
71	95.0
72	76.0
73	57.0
74	40.0
75	12.5
76	2.0
77	2.0
78	2.0
79	2.0
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.521592360484835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19069629512926	23.07087293195112
2	8.407541124504393	5.300381681612795
3	3.6623884603369996	3.4633294833745976
4	2.23268703877169	2.8151140281879687
5	1.4883593042321057	2.345772763696963
6	1.1466978274755968	2.16874448870036
7	0.9976077096733769	2.2012328492000766
8	0.7827555228137955	1.973896040644372
9	0.6564430380547142	1.862291686809504
>10	7.166133916145842	47.45928879364459
>50	0.24053364998743101	4.541087679358623
>100	0.024938271403379134	1.3954436843595321
>500	0.0016089207357018794	0.2677791260555453
>1k	0.0016089207357018794	1.1347647624039443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3060	0.7753843347025032	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1415	0.3585519064065497	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	549	0.13911307181427265	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	507	0.12847054173012062	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06917644554698804	0.0
2	0.0	0.0	0.0	0.2807600793628672	0.0
3	0.0	0.0	0.0	0.35525778995193125	0.0
4	0.0	0.0	0.0	0.4827147573883231	0.0
5	0.0	0.0	0.0	0.9030946957123274	0.0
6	0.0	0.0	0.0	1.1281081889201126	0.0
7	0.0	0.0	0.0	1.318913549714552	0.0
8	0.0	0.0	0.0	1.807709752865248	0.0
9	0.0	0.0	0.0	1.935420113875072	0.0
10	0.0	0.0	0.0	2.4150941483822086	0.0
11	0.0	0.0	0.0	2.8552387854339236	0.0
12	0.0	0.0	0.0	3.2817001695203007	0.0
13	0.0	0.0	0.0	3.408650349809828	0.0
14	0.0	0.0	0.0	3.462876574524317	0.0
15	0.0	0.0	0.0	3.562460248883168	0.0
16	0.0	0.0	0.0	3.7492113125026925	0.0
17	0.0	0.0	0.0	3.9836003679274685	0.0
18	0.0	0.0	0.0	4.260812937262285	0.0
19	0.0	0.0	0.0	4.4409757679725725	0.0
20	0.0	0.0	0.0	4.626459863724937	0.0
21	0.0	0.0	0.0	4.861609099870009	0.0
22	0.0	0.0	0.0	5.1722696208978745	0.0
23	0.0	0.0	0.0	5.441373595882861	0.0
24	0.0	0.0	0.0	5.661826004768867	0.0
25	0.0	0.0	0.0	5.844269377640044	0.0
26	0.0	0.0	0.0	6.030767047686137	0.0
27	0.0	0.0	0.0	6.210169697676127	0.0
28	0.0	0.0	0.0	6.387291805505229	0.0
29	0.0	0.0	0.0	6.619653712342547	0.0
30	0.0	0.0	0.0	6.871780317907577	0.0
31	0.0	0.0	0.0	7.093246301087312	0.0
32	0.0	0.0	0.0	7.319526762162258	0.0
33	0.0	0.0	0.0	7.5270560988032225	0.0
34	0.0	0.0	0.0	7.748015294836092	0.0
35	0.0	0.0	0.0	8.012051398352435	0.0
36	0.0	0.0	0.0	8.244920092336619	0.0
37	0.0	0.0	0.0	8.487164348537792	0.0
38	0.0	0.0	0.0	8.7438520384246	0.0
39	0.0	0.0	0.0	9.006621174073784	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	25	3.8861333E-5	45.000004	29
CAACGGG	35	1.2092096E-7	45.000004	3
GACCCGC	35	1.2092096E-7	45.000004	31
GTTACGG	25	3.8861333E-5	45.000004	2
AGCGGGC	35	1.2092096E-7	45.000004	4
GAGACGG	35	1.2092096E-7	45.000004	2
TACGCGG	25	3.8861333E-5	45.000004	2
CTACGGG	35	1.2092096E-7	45.000004	3
CGGGAGA	70	0.0	45.000004	6
GGACGGC	25	3.8861333E-5	45.000004	8
GCTAGCG	25	3.8861333E-5	45.000004	1
GGGCCGA	35	1.2092096E-7	45.000004	7
TATAGCG	50	2.1827873E-11	45.000004	1
ACCCGCA	35	1.2092096E-7	45.000004	32
TCACGAC	45	3.8380676E-10	45.0	25
CTCGTAC	20	7.027651E-4	45.0	29
CGACGGT	40	6.7957444E-9	45.0	28
AGCCAGC	20	7.027651E-4	45.0	36
GTCTCGT	20	7.027651E-4	45.0	27
TTCACGA	20	7.027651E-4	45.0	28
>>END_MODULE