FastQCFastQC Report
Sat 18 Jun 2016
SRR3549385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835200
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141881.6987547892720305No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG21470.2570641762452107No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19410.2323994252873563No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15870.19001436781609196No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15060.18031609195402298No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC13220.15828544061302682No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC11030.13206417624521072No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC10470.12535919540229884No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC9200.11015325670498084Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT8600.10296934865900384Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAC650.045.0000045
TACGGGT650.045.0000044
AGCGTTC253.889887E-545.021
TATCACG207.0321787E-445.01
AGCGTCA253.889887E-545.017
CGGCGAA207.0321787E-445.031
GTCGCGG207.0321787E-445.02
GTACCCG207.0321787E-445.01
GCGACGA207.0321787E-445.016
TACTTCG207.0321787E-445.017
GTCGAAG253.889887E-545.01
GTCTGCG253.889887E-545.01
ACGCATG351.2113924E-745.01
CCGATCA253.889887E-545.011
GGGTCCG207.0321787E-445.07
CGGCCCT207.0321787E-445.011
ATGGACG351.2113924E-745.038
GACCGTA207.0321787E-445.012
CCGGATG253.889887E-545.01
CCGTGTA207.0321787E-445.022