##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835200
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.373342911877394	33.0	31.0	34.0	31.0	34.0
2	32.707969348659006	34.0	31.0	34.0	31.0	34.0
3	32.67146791187739	34.0	31.0	34.0	31.0	34.0
4	36.253110632183905	37.0	37.0	37.0	35.0	37.0
5	36.290365181992335	37.0	37.0	37.0	35.0	37.0
6	36.31742935823755	37.0	37.0	37.0	35.0	37.0
7	36.367595785440614	37.0	37.0	37.0	35.0	37.0
8	36.186367337164754	37.0	37.0	37.0	35.0	37.0
9	38.12755028735632	39.0	39.0	39.0	37.0	39.0
10	37.773117816091954	39.0	38.0	39.0	35.0	39.0
11	37.84058908045977	39.0	38.0	39.0	35.0	39.0
12	37.684454022988504	39.0	37.0	39.0	35.0	39.0
13	37.705755507662836	39.0	37.0	39.0	35.0	39.0
14	39.097984913793105	41.0	39.0	41.0	35.0	41.0
15	39.074351053639845	41.0	38.0	41.0	35.0	41.0
16	39.0797653256705	41.0	38.0	41.0	35.0	41.0
17	38.9664846743295	41.0	38.0	41.0	35.0	41.0
18	38.851745689655175	40.0	38.0	41.0	35.0	41.0
19	38.781994731800765	40.0	37.0	41.0	35.0	41.0
20	38.694977250957855	40.0	37.0	41.0	35.0	41.0
21	38.65200670498084	40.0	37.0	41.0	35.0	41.0
22	38.65684985632184	40.0	37.0	41.0	35.0	41.0
23	38.64115301724138	40.0	37.0	41.0	35.0	41.0
24	38.57123563218391	40.0	37.0	41.0	35.0	41.0
25	38.46979885057471	40.0	36.0	41.0	35.0	41.0
26	38.44746048850575	40.0	36.0	41.0	35.0	41.0
27	38.44676484674329	40.0	36.0	41.0	35.0	41.0
28	38.32832495210728	40.0	36.0	41.0	35.0	41.0
29	38.30028376436782	40.0	36.0	41.0	35.0	41.0
30	38.13927562260536	40.0	36.0	41.0	35.0	41.0
31	38.001423611111115	40.0	36.0	41.0	34.0	41.0
32	37.753983477011495	40.0	36.0	41.0	34.0	41.0
33	37.46156130268199	40.0	36.0	41.0	33.0	41.0
34	37.117295258620686	40.0	35.0	41.0	33.0	41.0
35	36.93340517241379	40.0	35.0	41.0	33.0	41.0
36	36.81029454022988	40.0	35.0	41.0	32.0	41.0
37	36.7130471743295	40.0	35.0	41.0	31.0	41.0
38	36.61267480842912	40.0	35.0	41.0	31.0	41.0
39	36.50707255747126	40.0	35.0	41.0	31.0	41.0
40	36.4037344348659	40.0	35.0	40.0	31.0	41.0
41	36.26721623563218	39.0	35.0	40.0	30.0	41.0
42	36.23278376436782	39.0	35.0	40.0	30.0	41.0
43	36.13664870689655	39.0	35.0	40.0	30.0	41.0
44	36.0383596743295	39.0	35.0	40.0	30.0	41.0
45	35.997929837164754	39.0	35.0	40.0	30.0	41.0
46	35.88027059386973	39.0	35.0	40.0	29.0	41.0
47	35.81267001915709	38.0	35.0	40.0	29.0	41.0
48	35.69909722222222	38.0	35.0	40.0	29.0	41.0
49	35.5771719348659	38.0	35.0	40.0	28.0	41.0
50	35.481228448275864	38.0	35.0	40.0	27.0	41.0
51	35.42428999042146	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	6.0
12	3.0
13	5.0
14	9.0
15	8.0
16	24.0
17	72.0
18	141.0
19	296.0
20	533.0
21	831.0
22	1389.0
23	1988.0
24	3538.0
25	6559.0
26	11021.0
27	13429.0
28	12574.0
29	10607.0
30	9983.0
31	10470.0
32	12533.0
33	16973.0
34	33720.0
35	71261.0
36	44422.0
37	59498.0
38	151179.0
39	362116.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.2496408045977	24.198275862068964	29.377993295019156	17.174090038314176
2	32.13050766283525	26.231920498084293	29.77538314176245	11.862188697318008
3	27.297892720306514	25.27801724137931	36.233596743295024	11.190493295019158
4	25.41522988505747	25.637691570881227	35.52753831417624	13.419540229885058
5	22.240900383141764	30.406369731800766	34.41068007662835	12.942049808429118
6	19.669659961685824	40.987188697318004	30.106082375478927	9.23706896551724
7	81.94384578544062	4.031010536398467	12.277777777777779	1.7473659003831417
8	82.04597701149426	4.584650383141763	11.438936781609195	1.9304358237547892
9	75.96875	7.642121647509578	12.832255747126437	3.5568726053639845
10	30.154214559386972	38.26005747126437	21.23419540229885	10.351532567049809
11	28.250239463601535	25.11075191570881	30.958692528735632	15.680316091954023
12	27.083931992337163	22.40110153256705	34.169659961685824	16.345306513409962
13	21.623922413793103	28.518917624521073	33.82375478927203	16.033405172413794
14	16.33764367816092	31.30795019157088	34.250119731800766	18.104286398467433
15	16.095545977011493	30.670737547892717	38.98515325670498	14.248563218390805
16	18.5731561302682	27.648347701149422	37.36123084291188	16.4172653256705
17	17.714798850574713	29.56968390804598	32.754190613026815	19.96132662835249
18	17.699233716475096	29.884578544061302	36.1665469348659	16.2496408045977
19	21.059865900383144	29.75610632183908	32.54801245210728	16.6360153256705
20	21.327825670498086	27.666307471264368	36.43666187739464	14.569204980842912
21	19.517840038314176	30.462284482758623	34.98575191570881	15.03412356321839
22	20.045378352490424	25.977610153256702	33.95222701149425	20.02478448275862
23	17.73575191570881	29.528855363984675	34.84674329501915	17.888649425287355
24	15.927322796934865	29.266882183908045	39.55268199233716	15.253113026819923
25	16.09087643678161	31.979645593869733	35.58141762452107	16.348060344827587
26	15.566930076628353	33.97377873563218	33.762811302681996	16.69647988505747
27	16.7915469348659	34.39080459770115	33.00694444444445	15.810704022988507
28	15.14655172413793	31.5727969348659	36.949592911877396	16.331058429118777
29	16.021671455938698	27.946240421455936	37.473419540229884	18.55866858237548
30	15.677562260536398	33.9485153256705	34.149664750957854	16.22425766283525
31	19.619612068965516	30.569683908045974	32.99556992337165	16.815134099616856
32	19.50299329501916	30.559985632183906	33.78029214559387	16.156728927203066
33	18.950311302681992	31.834530651340998	31.38876915708812	17.82638888888889
34	16.24509099616858	30.45785440613027	33.36554118773946	19.931513409961685
35	18.685105363984675	29.274185823754788	34.050407088122604	17.99030172413793
36	20.855483716475096	31.898706896551726	30.95821360153257	16.28759578544061
37	17.31381704980843	34.313098659003835	32.025383141762454	16.347701149425287
38	17.451628352490424	33.35572318007663	31.125478927203066	18.067169540229884
39	18.78436302681992	32.827346743295024	31.081537356321835	17.30675287356322
40	18.66822318007663	31.202945402298848	32.83429118773946	17.294540229885058
41	17.41846264367816	30.310344827586206	32.47318007662835	19.79801245210728
42	17.12559865900383	29.773587164750957	33.23838601532567	19.86242816091954
43	18.982758620689655	28.541666666666664	32.07602969348659	20.399545019157088
44	15.96324233716475	31.285201149425284	33.38900862068965	19.362547892720304
45	16.44073275862069	33.624161877394634	31.386015325670495	18.549090038314176
46	17.526819923371647	34.37631704980843	30.646072796934863	17.450790229885058
47	17.294659961685824	30.882303639846743	32.947796934865906	18.87523946360153
48	17.115541187739463	30.189535440613025	34.69420498084291	18.0007183908046
49	17.853208812260537	29.825550766283527	33.70174808429119	18.61949233716475
50	17.363026819923373	31.699233716475096	32.95749521072797	17.980244252873565
51	17.49736590038314	30.534961685823752	30.971503831417625	20.99616858237548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	617.0
1	1165.5
2	1714.0
3	11967.0
4	22220.0
5	14726.0
6	7232.0
7	6979.0
8	6726.0
9	6941.0
10	7156.0
11	7326.0
12	7496.0
13	7451.0
14	7406.0
15	7171.0
16	6936.0
17	6092.0
18	5248.0
19	5360.5
20	5473.0
21	5502.5
22	5532.0
23	7004.5
24	8477.0
25	10087.0
26	14479.5
27	17262.0
28	22747.0
29	28232.0
30	31253.5
31	34275.0
32	38228.0
33	42181.0
34	44505.0
35	46829.0
36	49333.0
37	51837.0
38	55039.0
39	58241.0
40	60402.0
41	62563.0
42	66981.5
43	71400.0
44	71559.0
45	71718.0
46	70921.0
47	70124.0
48	64639.0
49	59154.0
50	50589.5
51	42025.0
52	33681.0
53	25337.0
54	20576.5
55	15816.0
56	13167.5
57	10519.0
58	9296.5
59	8074.0
60	7156.0
61	6238.0
62	5089.0
63	3940.0
64	3189.5
65	2439.0
66	1852.0
67	1265.0
68	1016.0
69	767.0
70	597.0
71	427.0
72	371.5
73	316.0
74	228.5
75	99.0
76	57.0
77	52.5
78	48.0
79	33.0
80	18.0
81	14.0
82	10.0
83	9.5
84	9.0
85	4.5
86	0.0
87	3.0
88	6.0
89	3.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	835200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.541191592381853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.63496447529539	20.339832748141916
2	9.068462507685771	4.8137560172963925
3	3.4312049950659187	2.7320480750034655
4	1.8882478565815703	2.004653925417433
5	1.2341325468626256	1.6377674188337563
6	0.8710237938516701	1.3870805636484291
7	0.6785713878859174	1.2607065250492018
8	0.5446855765491403	1.1565283395838164
9	0.4029462937482611	0.9625207305431498
>10	3.8856620375812057	21.488948148313934
>50	0.7405534809146398	14.20489131196779
>100	0.6127468214089588	24.408018510151614
>500	0.0031725057321469754	0.6151563567502925
>1k	0.0031725057321469754	1.2814354064256679
>5k	0.0	0.0
>10k+	4.532151045924251E-4	1.7066559228731226
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14188	1.6987547892720305	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	2147	0.2570641762452107	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1941	0.2323994252873563	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1587	0.19001436781609196	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1506	0.18031609195402298	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	1322	0.15828544061302682	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	1103	0.13206417624521072	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC	1047	0.12535919540229884	No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	920	0.11015325670498084	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	860	0.10296934865900384	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3946360153256704E-4	0.0	0.0	0.10835727969348659	0.0
2	2.3946360153256704E-4	0.0	0.0	0.42397030651340994	0.0
3	2.3946360153256704E-4	0.0	0.0	0.5739942528735632	0.0
4	2.3946360153256704E-4	0.0	0.0	0.7816091954022989	0.0
5	2.3946360153256704E-4	0.0	0.0	1.3773946360153257	0.0
6	2.3946360153256704E-4	0.0	0.0	1.76772030651341	0.0
7	2.3946360153256704E-4	0.0	0.0	2.0839319923371646	0.0
8	2.3946360153256704E-4	0.0	0.0	2.5880028735632186	0.0
9	2.3946360153256704E-4	0.0	0.0	2.7494013409961684	0.0
10	2.3946360153256704E-4	0.0	0.0	3.4280411877394634	0.0
11	2.3946360153256704E-4	0.0	0.0	4.085129310344827	0.0
12	2.3946360153256704E-4	0.0	0.0	4.697318007662835	0.0
13	2.3946360153256704E-4	0.0	0.0	4.889008620689655	0.0
14	2.3946360153256704E-4	0.0	0.0	4.965397509578544	0.0
15	2.3946360153256704E-4	0.0	0.0	5.101293103448276	0.0
16	2.3946360153256704E-4	0.0	0.0	5.389128352490421	0.0
17	2.3946360153256704E-4	0.0	0.0	5.747964559386973	0.0
18	2.3946360153256704E-4	0.0	0.0	6.149185823754789	0.0
19	2.3946360153256704E-4	0.0	0.0	6.4263649425287355	0.0
20	2.3946360153256704E-4	0.0	0.0	6.683668582375479	0.0
21	2.3946360153256704E-4	0.0	0.0	7.005866858237548	0.0
22	2.3946360153256704E-4	0.0	0.0	7.392480842911877	0.0
23	2.3946360153256704E-4	0.0	0.0	7.810464559386973	0.0
24	2.3946360153256704E-4	0.0	0.0	8.121527777777779	0.0
25	2.3946360153256704E-4	0.0	0.0	8.399904214559387	0.0
26	2.3946360153256704E-4	0.0	0.0	8.676724137931034	0.0
27	2.3946360153256704E-4	0.0	0.0	8.953544061302683	0.0
28	2.3946360153256704E-4	0.0	0.0	9.240900383141762	0.0
29	2.3946360153256704E-4	0.0	0.0	9.543702107279694	0.0
30	2.3946360153256704E-4	0.0	0.0	9.865900383141762	0.0
31	2.3946360153256704E-4	0.0	0.0	10.192887931034482	0.0
32	2.3946360153256704E-4	0.0	0.0	10.524545019157088	0.0
33	2.3946360153256704E-4	0.0	0.0	10.836087164750959	0.0
34	2.3946360153256704E-4	0.0	0.0	11.136973180076629	0.0
35	2.3946360153256704E-4	0.0	0.0	11.490421455938698	0.0
36	2.3946360153256704E-4	0.0	0.0	11.825909961685824	0.0
37	2.3946360153256704E-4	0.0	0.0	12.153735632183908	0.0
38	2.3946360153256704E-4	0.0	0.0	12.511494252873563	0.0
39	2.3946360153256704E-4	0.0	0.0	12.86374521072797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGAC	65	0.0	45.000004	5
TACGGGT	65	0.0	45.000004	4
AGCGTTC	25	3.889887E-5	45.0	21
TATCACG	20	7.0321787E-4	45.0	1
AGCGTCA	25	3.889887E-5	45.0	17
CGGCGAA	20	7.0321787E-4	45.0	31
GTCGCGG	20	7.0321787E-4	45.0	2
GTACCCG	20	7.0321787E-4	45.0	1
GCGACGA	20	7.0321787E-4	45.0	16
TACTTCG	20	7.0321787E-4	45.0	17
GTCGAAG	25	3.889887E-5	45.0	1
GTCTGCG	25	3.889887E-5	45.0	1
ACGCATG	35	1.2113924E-7	45.0	1
CCGATCA	25	3.889887E-5	45.0	11
GGGTCCG	20	7.0321787E-4	45.0	7
CGGCCCT	20	7.0321787E-4	45.0	11
ATGGACG	35	1.2113924E-7	45.0	38
GACCGTA	20	7.0321787E-4	45.0	12
CCGGATG	25	3.889887E-5	45.0	1
CCGTGTA	20	7.0321787E-4	45.0	22
>>END_MODULE