##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549384_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	861835
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9219955095813	31.0	31.0	34.0	30.0	34.0
2	32.19128139377027	33.0	31.0	34.0	30.0	34.0
3	31.93647507933653	33.0	31.0	34.0	30.0	34.0
4	35.780413884328205	37.0	35.0	37.0	35.0	37.0
5	30.817842162362865	37.0	35.0	37.0	0.0	37.0
6	33.27389349469446	37.0	35.0	37.0	17.0	37.0
7	35.44354545823737	37.0	35.0	37.0	32.0	37.0
8	35.824694982218176	37.0	35.0	37.0	35.0	37.0
9	37.772067739184415	39.0	38.0	39.0	35.0	39.0
10	37.34181832949462	39.0	37.0	39.0	34.0	39.0
11	37.3561563408309	39.0	37.0	39.0	34.0	39.0
12	37.169537092366866	39.0	37.0	39.0	34.0	39.0
13	37.11351592822292	39.0	37.0	39.0	33.0	39.0
14	38.429763237742726	40.0	38.0	41.0	34.0	41.0
15	38.50466968735315	40.0	38.0	41.0	34.0	41.0
16	38.540584914745864	40.0	38.0	41.0	34.0	41.0
17	38.42764218208822	40.0	37.0	41.0	34.0	41.0
18	38.30289672617148	40.0	37.0	41.0	34.0	41.0
19	38.20557415282508	40.0	37.0	41.0	34.0	41.0
20	38.094339403714166	40.0	36.0	41.0	34.0	41.0
21	37.98468384319504	40.0	36.0	41.0	34.0	41.0
22	38.02780230554573	40.0	35.0	41.0	34.0	41.0
23	38.05191829062407	40.0	35.0	41.0	34.0	41.0
24	38.01498082579612	40.0	35.0	41.0	34.0	41.0
25	37.94370152059269	40.0	35.0	41.0	34.0	41.0
26	37.91016377844947	40.0	35.0	41.0	34.0	41.0
27	37.387373453155185	40.0	35.0	41.0	33.0	41.0
28	37.461308719186384	40.0	35.0	41.0	33.0	41.0
29	37.59544228303562	40.0	35.0	41.0	33.0	41.0
30	37.49239587624081	40.0	35.0	41.0	33.0	41.0
31	37.405809696751696	40.0	35.0	41.0	33.0	41.0
32	37.19807967882483	40.0	35.0	41.0	33.0	41.0
33	36.964702060139125	40.0	35.0	41.0	32.0	41.0
34	36.70507231662673	40.0	35.0	41.0	31.0	41.0
35	36.51056060614851	40.0	35.0	41.0	30.0	41.0
36	36.35548683912814	40.0	35.0	41.0	30.0	41.0
37	36.25672199434926	40.0	35.0	41.0	30.0	41.0
38	36.19114795755568	39.0	35.0	41.0	30.0	41.0
39	36.11082979920751	39.0	35.0	41.0	29.0	41.0
40	35.98517929766138	39.0	35.0	41.0	29.0	41.0
41	35.888518103813375	39.0	35.0	40.0	28.0	41.0
42	35.823794577848425	39.0	35.0	40.0	27.0	41.0
43	35.74874308887432	39.0	35.0	40.0	27.0	41.0
44	35.64722597713019	39.0	35.0	40.0	27.0	41.0
45	35.63033295236327	39.0	35.0	40.0	27.0	41.0
46	35.51168611161069	38.0	35.0	40.0	26.0	41.0
47	35.411923396009676	38.0	35.0	40.0	26.0	41.0
48	35.31857025996855	38.0	35.0	40.0	25.0	41.0
49	35.25045745415306	38.0	35.0	40.0	25.0	41.0
50	35.1544529985438	38.0	35.0	40.0	24.0	41.0
51	35.16812266849223	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	0.0
11	2.0
12	2.0
13	10.0
14	11.0
15	17.0
16	52.0
17	96.0
18	243.0
19	467.0
20	907.0
21	1464.0
22	2374.0
23	3418.0
24	5562.0
25	8552.0
26	12648.0
27	14296.0
28	13791.0
29	13769.0
30	14880.0
31	17629.0
32	21889.0
33	30883.0
34	48177.0
35	78901.0
36	62079.0
37	87062.0
38	142908.0
39	279712.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.789501470699147	24.58614467966606	29.572481971607097	17.051871878027697
2	31.183811286383122	26.335319405686704	30.295938317659417	12.18493099027076
3	27.747422650507346	25.48028334890089	35.30292921498895	11.469364785602812
4	25.675448316673144	26.376858679445604	34.11279421234923	13.834898791532021
5	18.946782156677322	40.67356280494526	29.01309415375333	11.366560884624088
6	19.896848004548435	41.181664703800614	29.4565665121514	9.464920779499558
7	80.60139121757645	5.240678320096074	11.752597655003568	2.4053328073239078
8	80.81686169626437	5.513004229347844	11.169191318523847	2.5009427558639414
9	74.73193824804052	8.902632174372124	12.421055074347178	3.944374503240179
10	29.881821926470842	39.338968596076974	20.35760905509755	10.421600422354626
11	28.566604976590646	25.683106395075622	30.22341863581776	15.526869992515968
12	27.42056194051066	23.33230838849664	33.10204389471302	16.145085776279682
13	21.4619967859277	29.470142196592153	32.78875886915709	16.279102148323055
14	16.51557432687231	32.185743210707386	33.33027783740507	17.96840462501523
15	16.455005888598166	31.252153834550693	37.97873142770948	14.314108849141657
16	18.882616742183828	28.410078495303626	36.696583452749074	16.010721309763472
17	18.18689192246776	30.016998613423684	31.88498958617369	19.911119877934873
18	18.059721408390235	30.679306363747123	35.116930735001475	16.144041492861163
19	21.532775995405153	30.164938764380654	31.714307262991177	16.587977977223016
20	21.81217982560467	28.258889462600152	35.35247466162316	14.576456050172018
21	19.830826086199796	30.8061287833518	34.26317102461608	15.099874105832322
22	20.525854716970183	26.543479900444982	33.213318094530855	19.717347288053976
23	18.269158249548926	30.046934738087916	34.06789002535288	17.616016987010276
24	16.11723821845249	29.909321389825198	39.10400482691002	14.869435564812289
25	16.33909042914247	32.546020990096714	35.061583713819935	16.053304866940888
26	16.09124716448044	34.75119947553766	32.664837236826074	16.492716123155823
27	16.863784831203187	35.96836981556795	31.71871645964715	15.449128893581717
28	15.513758434039	32.467235607743945	36.057946126578756	15.961059831638307
29	16.21574895426619	28.699345002233606	36.77560089808374	18.309305145416467
30	15.884595079104468	34.49604622694599	33.455243753154605	16.16411494079493
31	20.145039363683303	30.931674856556068	32.52258262892549	16.400703150835135
32	19.742061995625612	31.050375071794484	33.3880615198965	15.819501412683401
33	19.16944658780393	32.17077514837527	31.09330672344474	17.566471540376057
34	16.45280129027018	30.871918638718547	33.06004049499034	19.615239576020933
35	18.968131951011504	29.328467746146302	33.8701723647798	17.83322793806239
36	21.107752644067602	32.2059326901321	30.732216723618787	15.954097942181509
37	17.35424994343465	34.83149326727274	31.704676649242604	16.10958014005001
38	17.523771951707694	33.951858534406234	30.76029634442788	17.764073169458193
39	18.82448496521956	33.13198001937726	30.8200525622654	17.22348245313778
40	18.813461973579628	31.588529126805014	32.35456903003475	17.243439869580605
41	17.38557844599024	30.8476680571107	32.159868188226284	19.606885308672776
42	16.908108860744804	30.483329175538245	32.744318808124525	19.86424315559243
43	18.927636960671126	29.350165634953328	31.54884635690126	20.173351047474284
44	15.988211200519823	31.8949682943951	33.11631576809946	19.00050473698562
45	16.379933513955688	34.64653907070379	31.089477684243505	17.884049731097022
46	17.475850945946732	35.2577929650107	30.04310569888668	17.22325039015589
47	17.586777051291723	31.552559364611554	32.16369722742753	18.6969663566692
48	17.16337814082742	30.9409573758318	34.2433296396642	17.652334843676574
49	17.948911334536195	30.750781762170256	33.06212906182738	18.238177841466175
50	17.47028143438129	32.862786960381044	32.31279769329396	17.354133911943702
51	17.627388073123047	31.51368881514443	30.523592102896725	20.335331008835798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	581.0
1	1092.0
2	1603.0
3	10666.5
4	19730.0
5	13643.0
6	7556.0
7	7059.5
8	6563.0
9	6768.5
10	6974.0
11	7248.0
12	7522.0
13	7329.5
14	7137.0
15	6963.0
16	6789.0
17	6055.0
18	5321.0
19	5390.5
20	5460.0
21	5676.5
22	5893.0
23	7504.0
24	9115.0
25	10905.5
26	15777.0
27	18858.0
28	24447.5
29	30037.0
30	33491.0
31	36945.0
32	41607.0
33	46269.0
34	48694.0
35	51119.0
36	53837.5
37	56556.0
38	60031.5
39	63507.0
40	66026.5
41	68546.0
42	73027.5
43	77509.0
44	76731.5
45	75954.0
46	72150.0
47	68346.0
48	61842.0
49	55338.0
50	47042.5
51	38747.0
52	31128.0
53	23509.0
54	19051.5
55	14594.0
56	12272.5
57	9951.0
58	8930.0
59	7909.0
60	7046.5
61	6184.0
62	5034.0
63	3884.0
64	3073.5
65	2263.0
66	1726.0
67	1189.0
68	945.0
69	701.0
70	550.0
71	399.0
72	333.5
73	268.0
74	218.0
75	107.5
76	47.0
77	50.0
78	53.0
79	36.0
80	19.0
81	12.5
82	6.0
83	7.5
84	9.0
85	8.5
86	8.0
87	4.0
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	861835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.277555779462006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64597455935953	20.36159734755231
2	9.090961106035458	4.959583973176036
3	3.8374090306378346	3.1402541664550423
4	2.345837943792265	2.5595490144548783
5	1.5643799301656671	2.1336230402682435
6	1.1577570752114201	1.8948469918887778
7	0.8208473278304871	1.5673496139922911
8	0.6688012126868436	1.4594609907549763
9	0.5465961006574865	1.3418825062068915
>10	4.089910102595554	22.339444704127104
>50	0.6796404001440101	13.352839559891269
>100	0.5458630807569386	21.995852489669055
>500	0.003011065063277045	0.5126365851605345
>1k	0.0025809129113803244	1.0971995211343917
>5k	0.0	0.0
>10k+	4.3015215189672073E-4	1.2838794952681545
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10942	1.2696165739381668	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2099	0.24355009949700349	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1756	0.20375129810230497	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1744	0.20235892021094526	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	1361	0.15791885917838103	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1358	0.15757076470554107	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	1033	0.11986053014788214	No Hit
TAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	890	0.10326802694251219	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001276346400413072	0.0	0.0	0.06393335151160025	0.0
2	0.001276346400413072	0.0	0.0	0.2529486502636816	0.0
3	0.001276346400413072	0.0	0.0	0.33347450498065173	0.0
4	0.001276346400413072	0.0	0.0	0.44927393294540136	0.0
5	0.001276346400413072	0.0	0.0	0.817557885210046	0.0
6	0.001276346400413072	0.0	0.0	1.0645889294354487	0.0
7	0.001276346400413072	0.0	0.0	1.2704287943747934	0.0
8	0.001276346400413072	0.0	0.0	1.6095888424118305	0.0
9	0.001276346400413072	0.0	0.0	1.716337814082742	0.0
10	0.001276346400413072	0.0	0.0	2.1607384244083843	0.0
11	0.001276346400413072	0.0	0.0	2.549908045043425	0.0
12	0.001276346400413072	0.0	0.0	2.9289829259661073	0.0
13	0.001276346400413072	0.0	0.0	3.0529045582971217	0.0
14	0.001276346400413072	0.0	0.0	3.100013343621459	0.0
15	0.001276346400413072	0.0	0.0	3.186688867358601	0.0
16	0.001276346400413072	0.0	0.0	3.3766324180382554	0.0
17	0.001276346400413072	0.0	0.0	3.607883179494915	0.0
18	0.001276346400413072	0.0	0.0	3.8789327423462727	0.0
19	0.001392377891359715	0.0	0.0	4.054488388148544	0.0
20	0.001392377891359715	0.0	0.0	4.229579907987028	0.0
21	0.001392377891359715	0.0	0.0	4.460134480498007	0.0
22	0.001392377891359715	0.0	0.0	4.729675633967059	0.0
23	0.001392377891359715	0.0	0.0	5.011168031003614	0.0
24	0.0015084093823063578	0.0	0.0	5.2311637378384495	0.0
25	0.001624440873253001	0.0	0.0	5.420411099572424	0.0
26	0.001624440873253001	0.0	0.0	5.60548132763232	0.0
27	0.0018565038551462867	0.0	0.0	5.794844720857241	0.0
28	0.0018565038551462867	0.0	0.0	6.005209813943504	0.0
29	0.0018565038551462867	0.0	0.0	6.225205520778339	0.0
30	0.0018565038551462867	0.0	0.0	6.468639588784396	0.0
31	0.0018565038551462867	0.0	0.0	6.7104492159172	0.0
32	0.0018565038551462867	0.0	0.0	6.95620391374219	0.0
33	0.0019725353460929296	0.0	0.0	7.196505131492687	0.0
34	0.0020885668370395727	0.0	0.0	7.426711609530827	0.0
35	0.002436661309879501	0.0	0.0	7.684185487941427	0.0
36	0.002436661309879501	0.0	0.0	7.945604437044214	0.0
37	0.002436661309879501	0.0	0.0	8.211780677275813	0.0
38	0.002436661309879501	0.0	0.0	8.493853231767101	0.0
39	0.002436661309879501	0.0	0.0	8.779870856950577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCA	35	1.211447E-7	45.000004	11
CGGCAGG	35	1.211447E-7	45.000004	2
TAGCGCG	45	3.8380676E-10	45.000004	1
GCGGGCG	45	3.8380676E-10	45.000004	5
CGTACAT	45	3.8380676E-10	45.000004	35
AACCGAT	20	7.032303E-4	45.0	23
TCGTTAC	25	3.8899903E-5	45.0	31
CCGGGAC	25	3.8899903E-5	45.0	5
GCACGAA	25	3.8899903E-5	45.0	45
TACGGGT	80	0.0	45.0	4
ATCACGC	20	7.032303E-4	45.0	10
ACGGGCC	20	7.032303E-4	45.0	5
CTCGGTT	20	7.032303E-4	45.0	23
CTAGTCG	20	7.032303E-4	45.0	1
CTACGCC	25	3.8899903E-5	45.0	23
CTACGAT	20	7.032303E-4	45.0	35
ACAGCGT	25	3.8899903E-5	45.0	15
TGTTACG	25	3.8899903E-5	45.0	1
AGCGATG	20	7.032303E-4	45.0	1
GGGCCGT	20	7.032303E-4	45.0	7
>>END_MODULE