FastQCFastQC Report
Sat 18 Jun 2016
SRR3549381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222697
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18180.8163558557142665No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.3677642716336547No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3880.17422776238566304No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3400.1526738123998078No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3030.13605930928571106No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG2310.10372838430692825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT351.2060264E-745.0000046
CGCGGGA207.021023E-445.0000044
CGACGGT207.021023E-445.00000428
ATAGCGG207.021023E-445.0000042
ACGGGTC207.021023E-445.0000045
GGCCCTC207.021023E-445.0000048
GAGACCG207.021023E-445.0000046
CTACGGG207.021023E-445.0000043
TTGGTCC207.021023E-445.00000416
CGGGACC207.021023E-445.0000046
CGGGAAT207.021023E-445.0000046
GGCGATA406.7720975E-945.0000048
AGGGCGA700.045.0000046
TTCGAAG207.021023E-445.0000042
CCCATAG351.2060264E-745.00000420
TCCGGGA207.021023E-445.0000044
ACGGGTA253.8806447E-545.05
GACCCGC453.8198777E-1045.031
ACGGGAC551.8189894E-1245.05
TGACCCG453.8198777E-1045.030