##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222697
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65266258638419	31.0	31.0	33.0	30.0	34.0
2	32.00243829059215	31.0	31.0	34.0	30.0	34.0
3	31.845233658289064	31.0	31.0	34.0	30.0	34.0
4	35.737396552266084	37.0	35.0	37.0	35.0	37.0
5	35.72202589168242	37.0	35.0	37.0	35.0	37.0
6	35.877142485080626	37.0	35.0	37.0	35.0	37.0
7	23.01490365833397	35.0	0.0	37.0	0.0	37.0
8	29.012133077679536	35.0	17.0	37.0	17.0	37.0
9	35.31011643623398	37.0	32.0	39.0	32.0	39.0
10	36.349057239208435	37.0	35.0	39.0	32.0	39.0
11	37.017638315738424	39.0	37.0	39.0	34.0	39.0
12	37.112507128519916	39.0	37.0	39.0	34.0	39.0
13	36.97263546433046	39.0	37.0	39.0	33.0	39.0
14	38.37654301584664	40.0	38.0	41.0	34.0	41.0
15	38.486607363368165	40.0	38.0	41.0	34.0	41.0
16	38.49136719399004	40.0	38.0	41.0	34.0	41.0
17	38.520756902877004	40.0	38.0	41.0	34.0	41.0
18	38.395330875584314	40.0	37.0	41.0	34.0	41.0
19	38.372254677880704	40.0	37.0	41.0	34.0	41.0
20	38.25686919895643	40.0	37.0	41.0	34.0	41.0
21	38.20052358136841	40.0	37.0	41.0	34.0	41.0
22	38.072636811452334	40.0	36.0	41.0	34.0	41.0
23	38.05309007305891	40.0	36.0	41.0	34.0	41.0
24	37.921395438645334	40.0	36.0	41.0	34.0	41.0
25	37.71913407005932	40.0	35.0	41.0	33.0	41.0
26	37.67602617008761	39.0	35.0	41.0	33.0	41.0
27	37.606873913882986	39.0	35.0	41.0	33.0	41.0
28	37.64352011926519	39.0	36.0	41.0	33.0	41.0
29	37.46913519266088	39.0	36.0	40.0	33.0	41.0
30	37.30935306717199	39.0	35.0	40.0	33.0	41.0
31	37.13281274556909	39.0	35.0	40.0	33.0	41.0
32	36.930686089170486	39.0	35.0	40.0	32.0	41.0
33	36.716583519310994	39.0	35.0	40.0	31.0	41.0
34	36.335276182436225	38.0	35.0	40.0	30.0	41.0
35	36.174959698603935	38.0	35.0	40.0	30.0	41.0
36	35.97969887335707	38.0	35.0	40.0	30.0	41.0
37	35.92423786579972	38.0	35.0	40.0	30.0	41.0
38	35.72604480527353	38.0	35.0	40.0	29.0	41.0
39	35.52150231031401	38.0	35.0	40.0	29.0	41.0
40	35.32931292294014	38.0	35.0	40.0	27.0	41.0
41	35.182463167442755	38.0	34.0	40.0	27.0	41.0
42	35.15853379255221	38.0	34.0	40.0	27.0	41.0
43	35.012779696179116	38.0	34.0	40.0	26.0	40.0
44	34.720400364620986	38.0	34.0	40.0	25.0	40.0
45	34.708545692128766	38.0	34.0	40.0	25.0	40.0
46	34.63604359286385	38.0	34.0	40.0	25.0	40.0
47	34.46558777172571	37.0	34.0	39.0	24.0	40.0
48	34.26518992173222	37.0	33.0	39.0	24.0	40.0
49	34.06887834142355	37.0	33.0	39.0	24.0	40.0
50	34.09094419772157	37.0	33.0	39.0	24.0	40.0
51	34.48909055802278	38.0	34.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	1.0
15	9.0
16	7.0
17	21.0
18	46.0
19	103.0
20	227.0
21	341.0
22	551.0
23	929.0
24	1565.0
25	2430.0
26	3202.0
27	3534.0
28	3459.0
29	3700.0
30	4184.0
31	5178.0
32	7021.0
33	9877.0
34	15466.0
35	21034.0
36	25278.0
37	45815.0
38	45464.0
39	23253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.389695415744264	25.064549589801388	29.483558377526414	17.06219661692793
2	29.95864335846464	26.803234888660377	31.027360045263297	12.210761707611688
3	28.34299519077491	25.53918553011491	35.03145529576061	11.086363983349573
4	26.10946712349066	26.748451932446326	33.46789584053669	13.674185103526316
5	21.92620466373593	31.65107747297898	33.45622078429435	12.966497078990734
6	20.011495439992455	42.1586280910834	28.982878080979983	8.846998387944158
7	54.12960210510245	37.91743939074168	6.7477334674468	1.205225036709071
8	84.51528309766184	4.393862512741528	9.446467621925756	1.644386767670871
9	78.20087383305567	8.262347494577835	10.737010377328836	2.799768295037652
10	30.258602495767796	40.983488776229585	19.02360606564076	9.734302662361864
11	29.455268818169976	25.835103301795716	29.73142880236375	14.978199077670556
12	28.32009411891494	23.379300125282334	32.72024320040234	15.580362555400388
13	21.6873150513927	30.157119314584392	31.91197007593277	16.243595558090142
14	16.32711711428533	32.75302316600583	32.679829544178865	18.24003017552998
15	16.27817168619245	31.88188435407751	37.97132426570632	13.868619694023717
16	18.817047378276314	28.88588530604364	36.654737154070325	15.642330161609722
17	18.019551228799667	30.519495098721585	31.1881165889078	20.272837083570952
18	18.03751285378788	31.23975626074891	34.95826167393364	15.764469211529567
19	21.83460037629604	30.651064001760236	31.02870716713741	16.485628454806307
20	21.764550038842014	28.50330269379471	35.386197389277804	14.34594987808547
21	19.88262078070203	31.421168673129856	33.821739852804484	14.874470693363628
22	20.90104491753369	26.575122251310074	32.79882530972577	19.725007521430463
23	18.05861776314903	30.73458555795543	33.7049892903811	17.50180738851444
24	15.771204820900147	30.454833248764018	39.451811205359746	14.322150724976087
25	16.248535004961898	32.955540487747925	35.07366511448291	15.722259392807267
26	15.80353574587893	35.733754832799725	31.959119341526826	16.50359007979452
27	16.795915526477682	36.32603941678604	31.663201569846024	15.214843486890258
28	15.240887843123168	33.123481681387716	35.933128870168886	15.702501605320233
29	15.852930214596514	29.124325877762164	36.76744635087136	18.25529755676996
30	15.67780437096144	35.49666138295532	32.917372034647975	15.908162211435267
31	20.559325002132944	31.452152476234524	32.11493643829957	15.873586083332958
32	19.839063840105613	31.480891076215666	33.1728761501053	15.50716893357342
33	19.236900362375785	32.741797150388194	30.789368514169478	17.231933973066543
34	16.1897106831255	31.20203684827366	32.87875454092332	19.729497927677517
35	18.809862728281026	29.342559621368945	34.20836383067576	17.639213819674264
36	21.557542310852863	32.35652029439103	30.481775686246337	15.60416170850977
37	16.973286573236283	35.69828062344801	31.48134011684037	15.847092686475344
38	17.14212584812548	34.669977592872826	30.810024382905922	17.37787217609577
39	18.625307031527143	33.608894596694164	30.851785161003516	16.914013210775177
40	18.73307678145642	31.926788416548046	32.36145974126279	16.978675060732744
41	16.9243411451434	30.89040265472817	32.38391177249806	19.80134442763037
42	16.374266379879387	30.745362532948356	32.86752852530569	20.012842561866574
43	18.61408101590951	29.525768196248713	31.483585319963897	20.37656546787788
44	15.548480671046311	32.40007723498745	33.27166508754047	18.77977700642577
45	15.941391217663462	35.67044010471627	30.841008186010587	17.547160491609677
46	17.14616721374783	36.10915279505337	29.812705155435417	16.93197483576339
47	17.297044863648814	31.789381985388214	32.20609168511475	18.707481465848215
48	16.70206603591427	31.270291023228868	34.56759633044001	17.460046610416843
49	17.442084985428632	31.3654876356664	33.303097931269846	17.889329447635127
50	17.109794923146698	33.45891502804258	32.34933564439575	17.08195440441497
51	17.37697409484636	31.96630399152211	30.523087423719225	20.133634489912303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	164.0
1	268.5
2	373.0
3	2421.5
4	4470.0
5	3187.5
6	1905.0
7	1814.5
8	1724.0
9	1783.0
10	1842.0
11	1867.5
12	1893.0
13	1857.5
14	1822.0
15	1723.5
16	1625.0
17	1481.5
18	1338.0
19	1389.5
20	1441.0
21	1659.0
22	1877.0
23	2258.0
24	2639.0
25	3359.5
26	5113.0
27	6146.0
28	7606.5
29	9067.0
30	9857.5
31	10648.0
32	11861.5
33	13075.0
34	13787.5
35	14500.0
36	15014.5
37	15529.0
38	16310.0
39	17091.0
40	17811.5
41	18532.0
42	19078.0
43	19624.0
44	19143.0
45	18662.0
46	17307.0
47	15952.0
48	14141.0
49	12330.0
50	10332.5
51	8335.0
52	6691.5
53	5048.0
54	4183.0
55	3318.0
56	2860.5
57	2403.0
58	2103.5
59	1804.0
60	1608.5
61	1413.0
62	1147.0
63	881.0
64	696.0
65	511.0
66	376.0
67	241.0
68	193.0
69	145.0
70	125.5
71	106.0
72	89.0
73	72.0
74	55.0
75	25.0
76	12.0
77	11.0
78	10.0
79	6.5
80	3.0
81	3.5
82	4.0
83	3.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	222697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.65476409650781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16098416644401	28.666753481187442
2	9.049405806024419	6.996052932908841
3	3.9694248574050626	4.603115443854206
4	2.2768723208995967	3.5204784976896857
5	1.6948758756084246	3.275751357225288
6	1.1326278126924014	2.626887654526105
7	0.9037789110509625	2.445475242145157
8	0.7411451738438484	2.2919033484959384
9	0.616846531835554	2.14596514546671
>10	5.363428319180326	39.017588921269706
>50	0.0673768339858044	1.7018639676331517
>100	0.020910051926628953	1.5240438802498462
>500	0.0011616695514793862	0.3677642716336547
>1k	0.0011616695514793862	0.8163558557142665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1818	0.8163558557142665	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	819	0.3677642716336547	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	388	0.17422776238566304	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	340	0.1526738123998078	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	303	0.13605930928571106	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	231	0.10372838430692825	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04670022496935298	0.0
2	0.0	0.0	0.0	0.18231049363035873	0.0
3	0.0	0.0	0.0	0.23664440921970212	0.0
4	0.0	0.0	0.0	0.3237582904125336	0.0
5	0.0	0.0	0.0	0.580609527743975	0.0
6	0.0	0.0	0.0	0.7386718276402466	0.0
7	0.0	0.0	0.0	0.8720368931777258	0.0
8	0.0	0.0	0.0	1.2056740773337764	0.0
9	0.0	0.0	0.0	1.3058101366430621	0.0
10	0.0	0.0	0.0	1.670880164528485	0.0
11	0.0	0.0	0.0	1.991046129943376	0.0
12	0.0	0.0	0.0	2.335011248467649	0.0
13	0.0	0.0	0.0	2.4463733233945675	0.0
14	0.0	0.0	0.0	2.483643695245109	0.0
15	0.0	0.0	0.0	2.5590825201956022	0.0
16	0.0	0.0	0.0	2.716246738842463	0.0
17	0.0	0.0	0.0	2.9349295230739525	0.0
18	0.0	0.0	0.0	3.1616950385501377	0.0
19	0.0	0.0	0.0	3.3062861197052498	0.0
20	0.0	0.0	0.0	3.4490810383615407	0.0
21	0.0	0.0	0.0	3.634085775740131	0.0
22	0.0	0.0	0.0	3.8599532099669056	0.0
23	0.0	0.0	0.0	4.104231309806599	0.0
24	0.0	0.0	0.0	4.307197672173402	0.0
25	0.0	0.0	0.0	4.477384068936717	0.0
26	0.0	0.0	0.0	4.643080059452979	0.0
27	0.0	0.0	0.0	4.796651953102197	0.0
28	0.0	0.0	0.0	4.97671724360903	0.0
29	0.0	0.0	0.0	5.171151834106432	0.0
30	0.0	0.0	0.0	5.379506683969699	0.0
31	0.0	0.0	0.0	5.5963933057023665	0.0
32	0.0	0.0	0.0	5.805646236815044	0.0
33	0.0	0.0	0.0	6.022981899172418	0.0
34	0.0	0.0	0.0	6.229540586536864	0.0
35	0.0	0.0	0.0	6.450917614516586	0.0
36	0.0	0.0	0.0	6.686214901862171	0.0
37	0.0	0.0	0.0	6.9188179454595256	0.0
38	0.0	0.0	0.0	7.154564273429817	0.0
39	0.0	0.0	0.0	7.395699088896572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGT	35	1.2060264E-7	45.000004	6
CGCGGGA	20	7.021023E-4	45.000004	4
CGACGGT	20	7.021023E-4	45.000004	28
ATAGCGG	20	7.021023E-4	45.000004	2
ACGGGTC	20	7.021023E-4	45.000004	5
GGCCCTC	20	7.021023E-4	45.000004	8
GAGACCG	20	7.021023E-4	45.000004	6
CTACGGG	20	7.021023E-4	45.000004	3
TTGGTCC	20	7.021023E-4	45.000004	16
CGGGACC	20	7.021023E-4	45.000004	6
CGGGAAT	20	7.021023E-4	45.000004	6
GGCGATA	40	6.7720975E-9	45.000004	8
AGGGCGA	70	0.0	45.000004	6
TTCGAAG	20	7.021023E-4	45.000004	2
CCCATAG	35	1.2060264E-7	45.000004	20
TCCGGGA	20	7.021023E-4	45.000004	4
ACGGGTA	25	3.8806447E-5	45.0	5
GACCCGC	45	3.8198777E-10	45.0	31
ACGGGAC	55	1.8189894E-12	45.0	5
TGACCCG	45	3.8198777E-10	45.0	30
>>END_MODULE