##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	838315
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41439315770325	33.0	31.0	34.0	31.0	34.0
2	32.7690665203414	34.0	31.0	34.0	31.0	34.0
3	32.75333854219476	34.0	31.0	34.0	31.0	34.0
4	36.292332834316454	37.0	37.0	37.0	35.0	37.0
5	36.327104966510205	37.0	37.0	37.0	35.0	37.0
6	36.34642944477911	37.0	37.0	37.0	35.0	37.0
7	36.37224432343451	37.0	37.0	37.0	35.0	37.0
8	36.16587559568897	37.0	37.0	37.0	35.0	37.0
9	38.11264739387939	39.0	39.0	39.0	37.0	39.0
10	37.90984892313748	39.0	38.0	39.0	35.0	39.0
11	37.92465004204863	39.0	38.0	39.0	35.0	39.0
12	37.771911512975436	39.0	37.0	39.0	35.0	39.0
13	37.75244746903014	39.0	38.0	39.0	35.0	39.0
14	39.173274962275514	41.0	39.0	41.0	35.0	41.0
15	39.15298664583122	41.0	39.0	41.0	35.0	41.0
16	39.1705253991638	41.0	39.0	41.0	35.0	41.0
17	39.05672330806439	41.0	38.0	41.0	35.0	41.0
18	38.9503730697888	40.0	38.0	41.0	35.0	41.0
19	38.909283503217765	40.0	38.0	41.0	35.0	41.0
20	38.85476461711886	40.0	38.0	41.0	35.0	41.0
21	38.8312961118434	40.0	38.0	41.0	35.0	41.0
22	38.8341685404651	40.0	38.0	41.0	35.0	41.0
23	38.81103880999385	40.0	38.0	41.0	35.0	41.0
24	38.75280413686979	40.0	38.0	41.0	35.0	41.0
25	38.6494372640356	40.0	37.0	41.0	35.0	41.0
26	38.621678008863014	40.0	37.0	41.0	35.0	41.0
27	38.607091606377075	40.0	37.0	41.0	35.0	41.0
28	38.48855740383985	40.0	37.0	41.0	35.0	41.0
29	38.446625671734374	40.0	37.0	41.0	35.0	41.0
30	38.31626178703709	40.0	37.0	41.0	35.0	41.0
31	38.19550884810602	40.0	37.0	41.0	35.0	41.0
32	37.9864168003674	40.0	37.0	41.0	34.0	41.0
33	37.777460739698085	40.0	37.0	41.0	34.0	41.0
34	37.490233384825515	40.0	36.0	41.0	34.0	41.0
35	37.341860756398255	40.0	36.0	41.0	33.0	41.0
36	37.236081902387525	40.0	36.0	41.0	33.0	41.0
37	37.16257134847879	40.0	36.0	41.0	33.0	41.0
38	37.05742948652953	40.0	36.0	41.0	33.0	41.0
39	36.96276459326148	40.0	36.0	41.0	33.0	41.0
40	36.83734872929627	40.0	36.0	41.0	32.0	41.0
41	36.70578123974878	40.0	35.0	40.0	32.0	41.0
42	36.66240971472537	40.0	35.0	40.0	32.0	41.0
43	36.582518504380815	40.0	35.0	40.0	31.0	41.0
44	36.484943010682144	39.0	35.0	40.0	31.0	41.0
45	36.41652958613409	39.0	35.0	40.0	31.0	41.0
46	36.29299964810364	39.0	35.0	40.0	31.0	41.0
47	36.222911435438945	39.0	35.0	40.0	31.0	41.0
48	36.140146603603654	39.0	35.0	40.0	31.0	41.0
49	35.99034730381778	38.0	35.0	40.0	30.0	41.0
50	35.91999904570478	38.0	35.0	40.0	30.0	41.0
51	35.867356542588404	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	1.0
14	6.0
15	6.0
16	18.0
17	61.0
18	138.0
19	271.0
20	423.0
21	760.0
22	1228.0
23	1826.0
24	3134.0
25	5423.0
26	8906.0
27	10934.0
28	10621.0
29	9431.0
30	8881.0
31	9869.0
32	11872.0
33	15761.0
34	30116.0
35	69453.0
36	41419.0
37	57538.0
38	155924.0
39	384282.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.074751137698833	25.4145518092841	29.6845457852955	15.826151267721562
2	30.700273763442144	27.1485062297585	29.732856980967775	12.418363025831578
3	25.953847897270123	26.330198075902256	35.457912598486246	12.258041428341375
4	25.052635345902196	26.747821522935887	35.30641823181024	12.893124899351676
5	23.19605398925225	31.901970023201304	31.538264256275983	13.363711731270463
6	20.46629250341459	41.77916415667141	27.898224414450418	9.85631892546358
7	84.1724172894437	3.8714564334408905	10.20356309978946	1.7525631773259456
8	83.89853456039795	5.059911846978761	9.147993296076057	1.8935602965472407
9	76.88625397374496	8.666909216702551	10.71792822507053	3.7289085844819665
10	26.277950412434464	49.742519220102224	16.114825572726243	7.8647047947370625
11	27.732892767038642	24.01841789780691	33.68900711546376	14.55968221969069
12	29.863237565831458	22.6639151154399	31.030459910654113	16.44238740807453
13	22.479139702856322	29.236742751829564	30.965448548576607	17.3186689967375
14	15.290075926113692	32.22583396455986	32.35526025420039	20.128829855126057
15	14.598569750034295	32.25279280461402	37.02951754412124	16.119119901230444
16	17.987272087461157	29.59388773909569	35.57278588597365	16.84605428746951
17	17.1453451268318	30.6275087526765	32.003363890661625	20.223782229830075
18	16.821600472376137	30.259508657246975	36.344572147701044	16.574318722675844
19	19.5729528876377	30.731884792709184	31.342514448626112	18.352647871027
20	20.78359566511395	29.369986222362716	34.85002654133589	14.996391571187443
21	19.47609192248737	29.981212312794113	34.629345770981075	15.91334999373744
22	20.098888842499537	27.81985291924873	32.39736853092215	19.683889707329584
23	17.340737073772985	29.94649982405182	33.41643654234983	19.296326559825363
24	15.089316068542255	29.020952744493417	39.86496722592343	16.024763961040897
25	15.330752760000715	33.94595110429851	34.07585454155061	16.64744159415017
26	15.602846185503063	34.91527647721918	31.318060633532742	18.163816703745013
27	15.541890578123974	34.86123951020797	31.852943106111663	17.743926805556384
28	14.054025038320916	33.367409625260194	35.654020266844796	16.924545069574087
29	15.350315812075413	28.231750594943428	35.53175119137794	20.886182401603214
30	15.25655630640034	33.48836654479521	33.42478662555245	17.830290523252
31	18.710031432098912	32.76536862635167	31.249470664368406	17.275129277181012
32	18.722317983097046	31.66208406148047	31.965907803152753	17.64969015226973
33	19.351317822059727	32.19386507458414	29.963915711874417	18.490901391481724
34	16.64529442989807	31.024495565509387	30.75240214000704	21.57780786458551
35	18.06480857434258	29.86109040157936	31.830994315979076	20.243106708098985
36	20.038410382732028	31.320207797784843	30.627866613385184	18.013515206097946
37	16.44763603180189	34.31001473193251	32.61053422639461	16.63181500987099
38	16.168504679028768	34.42321800277938	31.180403547592494	18.227873770599356
39	17.504160130738445	33.06740306448053	32.06646666229281	17.361970142488207
40	18.982482718309942	31.47396861561585	31.66279978289784	17.880748883176373
41	16.53936766012776	29.74705212241222	32.48528297835539	21.22829723910463
42	15.84130070438916	29.05339878208072	32.76107429784747	22.34422621568265
43	17.999439351556397	29.707926018262825	31.765386519387107	20.527248110793675
44	15.73358463107543	31.361838926895018	31.631904475048163	21.272671966981385
45	15.497873710955906	34.52628188688023	29.55154088856814	20.424303513595724
46	17.135682887697346	36.51777673070386	28.675736447516748	17.67080393408206
47	16.56990510726875	30.70623810858687	33.16486046414534	19.558996319999046
48	17.113614810661865	30.134495983013544	34.31991554487276	18.431973661451842
49	17.256878381038156	29.843197366145187	33.374447552530974	19.525476700285694
50	16.879812480988647	31.411581565402024	33.43755032416216	18.271055629447165
51	16.146675175799075	30.703494509820295	31.614488587225566	21.535341727155068
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1413.0
1	1631.0
2	1849.0
3	9789.5
4	17730.0
5	11654.0
6	5578.0
7	5395.5
8	5213.0
9	5335.5
10	5458.0
11	5475.0
12	5492.0
13	5477.5
14	5463.0
15	4968.0
16	4473.0
17	4200.0
18	3927.0
19	4526.5
20	5126.0
21	5370.0
22	5614.0
23	6327.5
24	7041.0
25	9177.0
26	14850.5
27	18388.0
28	22174.5
29	25961.0
30	30969.5
31	35978.0
32	39856.5
33	43735.0
34	49526.0
35	55317.0
36	58377.5
37	61438.0
38	62094.0
39	62750.0
40	70834.5
41	78919.0
42	76059.0
43	73199.0
44	73091.0
45	72983.0
46	70436.0
47	67889.0
48	59798.0
49	51707.0
50	43017.5
51	34328.0
52	27923.5
53	21519.0
54	17430.5
55	13342.0
56	11791.5
57	10241.0
58	9128.5
59	8016.0
60	7177.5
61	6339.0
62	5398.0
63	4457.0
64	3485.5
65	2514.0
66	1996.0
67	1478.0
68	1183.5
69	889.0
70	738.5
71	588.0
72	413.0
73	238.0
74	198.0
75	131.0
76	104.0
77	73.5
78	43.0
79	53.0
80	63.0
81	37.5
82	12.0
83	17.0
84	22.0
85	13.0
86	4.0
87	4.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	838315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.07669041155528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51086170961673	15.360848836652941
2	8.360929103194156	3.3571957031558317
3	3.0613272945476346	1.8438396102323087
4	1.6392006300260478	1.3163889428583728
5	1.1137501652076296	1.1180208631346062
6	0.8371506485219562	1.0084328638924827
7	0.6788639332454242	0.9540538713537374
8	0.5481968673349488	0.8804783032054564
9	0.413854290193547	0.747794201373882
>10	4.466889494649441	19.33498110759023
>50	1.2501601456881133	18.117962583407618
>100	1.1074723353636344	32.482330452299905
>500	0.007164241522567985	0.902320251975711
>1k	0.0035821207612839927	1.2229507878464636
>5k	0.0	0.0
>10k+	5.970201268806655E-4	1.35240162102045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11283	1.3459141253586062	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCG	2273	0.271139130279191	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2205	0.26302762088236525	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTC	1960	0.23380232967321352	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1454	0.1734431568085982	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGC	1181	0.14087783231840062	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1130	0.13479420027078126	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.192869028944967E-4	0.0	0.0	0.07383859289169345	0.0
2	1.192869028944967E-4	0.0	0.0	0.29356506802335636	0.0
3	1.192869028944967E-4	0.0	0.0	0.40688762577312826	0.0
4	1.192869028944967E-4	0.0	0.0	0.6023988596172083	0.0
5	1.192869028944967E-4	0.0	0.0	1.1804631910439394	0.0
6	1.192869028944967E-4	0.0	0.0	1.517329404817998	0.0
7	1.192869028944967E-4	0.0	0.0	1.787991387485611	0.0
8	1.192869028944967E-4	0.0	0.0	2.246172381503373	0.0
9	1.192869028944967E-4	0.0	0.0	2.3791772782307365	0.0
10	1.192869028944967E-4	0.0	0.0	2.9267041625164767	0.0
11	1.192869028944967E-4	0.0	0.0	3.3558984391308755	0.0
12	1.192869028944967E-4	0.0	0.0	3.8355510756696467	0.0
13	1.192869028944967E-4	0.0	0.0	3.9759517603764696	0.0
14	1.192869028944967E-4	0.0	0.0	4.034879490406351	0.0
15	1.192869028944967E-4	0.0	0.0	4.141879842302714	0.0
16	1.192869028944967E-4	0.0	0.0	4.303275021918968	0.0
17	2.385738057889934E-4	0.0	0.0	4.503199871170145	0.0
18	2.385738057889934E-4	0.0	0.0	4.718035583283133	0.0
19	2.385738057889934E-4	0.0	0.0	4.8987552411682955	0.0
20	2.385738057889934E-4	0.0	0.0	5.064206175482963	0.0
21	2.385738057889934E-4	0.0	0.0	5.266993910403607	0.0
22	2.385738057889934E-4	0.0	0.0	5.519166423122574	0.0
23	2.385738057889934E-4	0.0	0.0	5.741397923215021	0.0
24	2.385738057889934E-4	0.0	0.0	5.941919206980669	0.0
25	2.385738057889934E-4	0.0	0.0	6.1160780852066345	0.0
26	2.385738057889934E-4	0.0	0.0	6.295127726451274	0.0
27	3.578607086834901E-4	0.0	0.0	6.455330037038584	0.0
28	3.578607086834901E-4	0.0	0.0	6.62686460340087	0.0
29	3.578607086834901E-4	0.0	0.0	6.812355737401812	0.0
30	3.578607086834901E-4	0.0	0.0	7.041386590959245	0.0
31	3.578607086834901E-4	0.0	0.0	7.247514359160936	0.0
32	3.578607086834901E-4	0.0	0.0	7.445292044160012	0.0
33	3.578607086834901E-4	0.0	0.0	7.638894687557779	0.0
34	3.578607086834901E-4	0.0	0.0	7.8390581106147454	0.0
35	3.578607086834901E-4	0.0	0.0	8.05961959406667	0.0
36	3.578607086834901E-4	0.0	0.0	8.281731807256222	0.0
37	3.578607086834901E-4	0.0	0.0	8.496567519369211	0.0
38	3.578607086834901E-4	0.0	0.0	8.715816846889295	0.0
39	3.578607086834901E-4	0.0	0.0	8.936259043438326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTAC	45	3.8380676E-10	45.000004	30
TCGAGAG	45	3.8380676E-10	45.000004	1
TCGCCCT	20	7.0321944E-4	45.0	30
GCCCCGA	25	3.8898987E-5	45.0	1
CGGGTTG	20	7.0321944E-4	45.0	6
CGGAATA	20	7.0321944E-4	45.0	32
CGGGTAT	40	6.8121153E-9	45.0	6
TAATACG	25	3.8898987E-5	45.0	1
TCCGCCA	20	7.0321944E-4	45.0	23
CACGGGC	35	1.2114106E-7	45.0	4
CAACGAG	20	7.0321944E-4	45.0	1
TCCGATT	20	7.0321944E-4	45.0	45
TCCGATA	20	7.0321944E-4	45.0	12
TTTCGGA	20	7.0321944E-4	45.0	29
TTTCGCG	35	1.2114106E-7	45.0	1
CGGCATA	20	7.0321944E-4	45.0	6
GACCGTC	20	7.0321944E-4	45.0	9
CGCATGG	80	0.0	45.0	2
TTATGCG	40	6.8121153E-9	45.0	1
TAGCGGA	40	6.8121153E-9	45.0	3
>>END_MODULE