FastQCFastQC Report
Sat 18 Jun 2016
SRR3549375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237790
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.6110433575844233No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.2897514613734808No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4050.1703183481222928No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3160.132890365448505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.13204928718617268No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTC3120.13120820892384036No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA2440.10261154800454184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT207.0219894E-445.00000444
GTTGAGC207.0219894E-445.00000442
CGGAACA207.0219894E-445.00000415
CCGGGAT302.1582164E-645.0000045
ACACGGG453.8198777E-1045.0000043
GATCGTA207.0219894E-445.0000049
CGCGGCC207.0219894E-445.00000435
CGGCCCT207.0219894E-445.00000437
GACGTAC207.0219894E-445.0000049
TAGCGAA207.0219894E-445.0000041
AGGACGC207.0219894E-445.0000047
TATATCG207.0219894E-445.00000429
ACGGGAC406.7757355E-945.0000045
AGAACGC207.0219894E-445.00000410
ATCGTAC207.0219894E-445.00000410
AGGCCGA207.0219894E-445.0000047
GTTCGAA207.0219894E-445.0000041
CGAGGAT207.0219894E-445.0000045
GTGTCAT207.0219894E-445.00000429
CGGGCTG207.0219894E-445.0000046