##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237790
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669212330207326	31.0	31.0	33.0	30.0	34.0
2	32.04415660877245	33.0	31.0	34.0	30.0	34.0
3	31.950275453130914	33.0	31.0	34.0	30.0	34.0
4	35.80235501913453	37.0	35.0	37.0	35.0	37.0
5	35.75937591992935	37.0	35.0	37.0	35.0	37.0
6	35.89905378695487	37.0	35.0	37.0	35.0	37.0
7	22.828634509441102	35.0	0.0	37.0	0.0	37.0
8	28.891467261028637	35.0	17.0	37.0	17.0	37.0
9	35.268825434206654	37.0	32.0	39.0	32.0	39.0
10	36.46973800412128	37.0	35.0	39.0	32.0	39.0
11	37.09868371251945	39.0	37.0	39.0	34.0	39.0
12	37.168930568989445	39.0	37.0	39.0	34.0	39.0
13	36.9809243450103	39.0	37.0	39.0	33.0	39.0
14	38.40748139114345	40.0	38.0	41.0	34.0	41.0
15	38.48999957946087	40.0	38.0	41.0	34.0	41.0
16	38.51116110854115	40.0	38.0	41.0	34.0	41.0
17	38.5636864460238	40.0	38.0	41.0	34.0	41.0
18	38.422898355692	40.0	38.0	41.0	34.0	41.0
19	38.418533159510496	40.0	37.0	41.0	34.0	41.0
20	38.35612935783675	40.0	37.0	41.0	34.0	41.0
21	38.32366373691072	40.0	37.0	41.0	34.0	41.0
22	38.18086126414063	40.0	37.0	41.0	34.0	41.0
23	38.19660624921149	40.0	37.0	41.0	34.0	41.0
24	38.09569788468817	40.0	36.0	41.0	34.0	41.0
25	37.87266075108289	40.0	36.0	41.0	33.0	41.0
26	37.812544682282685	40.0	36.0	41.0	33.0	41.0
27	37.75502333992178	40.0	36.0	41.0	33.0	41.0
28	37.77319063038816	40.0	36.0	41.0	33.0	41.0
29	37.59852811304092	40.0	36.0	41.0	33.0	41.0
30	37.47962908448631	39.0	36.0	40.0	33.0	41.0
31	37.294049371294	39.0	36.0	40.0	33.0	41.0
32	37.13250767483915	39.0	35.0	40.0	32.0	41.0
33	36.97665166743766	39.0	35.0	40.0	32.0	41.0
34	36.624761344043065	39.0	35.0	40.0	31.0	41.0
35	36.510387316539806	39.0	35.0	40.0	31.0	41.0
36	36.358126918709786	39.0	35.0	40.0	30.0	41.0
37	36.337263972412636	39.0	35.0	40.0	30.0	41.0
38	36.119353210816264	38.0	35.0	40.0	30.0	41.0
39	35.9288868329198	38.0	35.0	40.0	30.0	41.0
40	35.70938643340763	38.0	35.0	40.0	29.0	41.0
41	35.580264098574375	38.0	35.0	40.0	29.0	41.0
42	35.56592371420161	38.0	35.0	40.0	29.0	41.0
43	35.40039110139198	38.0	35.0	40.0	28.0	41.0
44	35.12358803986711	38.0	34.0	40.0	27.0	40.0
45	35.08237941040414	38.0	34.0	40.0	27.0	40.0
46	34.96755961142184	38.0	34.0	40.0	27.0	40.0
47	34.796442238950334	38.0	34.0	40.0	27.0	40.0
48	34.664535935068756	37.0	34.0	39.0	26.0	40.0
49	34.405790823836156	37.0	33.0	39.0	25.0	40.0
50	34.43295344631818	37.0	34.0	39.0	26.0	40.0
51	34.84940914252071	38.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	2.0
15	4.0
16	5.0
17	18.0
18	40.0
19	109.0
20	204.0
21	329.0
22	522.0
23	853.0
24	1371.0
25	2138.0
26	2817.0
27	3295.0
28	3413.0
29	3712.0
30	4439.0
31	5339.0
32	7107.0
33	10224.0
34	15982.0
35	21599.0
36	27023.0
37	50005.0
38	50874.0
39	26362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.280415492661593	26.194120862946296	29.99074813911434	15.534715505277767
2	28.68118928466294	27.033096429622777	31.43193574162076	12.853778544093528
3	26.518777072206568	26.455696202531648	34.861852895411914	12.163673829849868
4	25.937591992934944	27.54236931746499	33.52495899743471	12.995079692165357
5	22.522814247865764	33.23478699693007	30.872198158038604	13.370200597165566
6	20.7666428361159	42.75369023087598	26.9296438033559	9.550023129652214
7	55.24538458303545	38.17906556205055	5.376172252828126	1.1993776020858742
8	86.38504562849573	4.5456074687749695	7.415366499852811	1.6539804028764877
9	79.3998906598259	8.778754363093485	8.821649354472434	2.9997056226081837
10	26.136507001976533	51.95214264687329	14.215904789940703	7.69544556120947
11	28.199671979477692	24.515328651331007	32.91938264855545	14.365616720635854
12	30.851171201480298	23.43454308423399	29.645064973295764	16.069220740989948
13	22.39370873459775	30.472685983430758	29.46423314689432	17.669372135077168
14	15.3715463223853	32.807098700534084	31.27086925438412	20.550485722696497
15	14.784053156146179	33.312166197064634	35.93717145380378	15.966609192985407
16	18.294713823121242	30.387316539804026	34.60742672105639	16.710542916018337
17	17.440178308591612	31.299886454434585	30.941166575549854	20.318768661423945
18	17.10374700365869	31.226292106480507	35.457336305143194	16.21262458471761
19	20.33222591362126	31.11106438454098	30.309096261407127	18.247613440430634
20	20.976071323436646	30.21783926994407	33.73438748475546	15.071701921863829
21	19.63244879936078	30.659825896799695	33.72303292821397	15.984692375625553
22	20.516842592203204	28.50624500609782	31.15942638462509	19.817486017073886
23	17.411581647672318	30.69262794903066	32.44459396946886	19.45119643382817
24	14.996425417385087	29.63581311241011	39.811598469237566	15.55616300096724
25	15.302577904874049	34.796669330081166	33.57836746709281	16.322385297951975
26	15.787459523108623	36.36822406324908	29.82715841709071	18.017157996551582
27	15.448925522519872	36.34004794146095	30.874300853694436	17.33672568232474
28	14.153244459396946	34.07081878968838	35.14487573068674	16.631061020227932
29	15.3702847049918	29.022246520038692	34.98507086084361	20.62239791412591
30	15.236973800412128	34.40178308591614	32.749064300433155	17.61217881323857
31	19.251440346524245	33.44043063207031	30.66403128811136	16.644097733294082
32	19.06892636359813	32.03877370789352	31.685100298582785	17.207199629925565
33	19.541191807897725	32.9833045964927	29.41923545985954	18.05626813575003
34	16.56671853315951	31.196854367298876	30.78851087093654	21.44791622860507
35	18.366205475419488	29.888977669372135	32.07451953404264	19.670297321165734
36	20.574876992304134	31.46053240254006	30.755288279574415	17.209302325581397
37	16.306404810967663	35.111653139324616	32.36469153454729	16.217250515160437
38	16.33710416754279	34.58051221666176	30.99962151478195	18.0827621010135
39	17.26775726481349	33.6965389629505	32.043399638336346	16.992304133899662
40	19.20686319862063	31.937844316413646	31.18549981075739	17.669792674208335
41	16.3295344631818	30.201438243828587	32.30161066487236	21.167416628117248
42	15.39425543546827	29.32503469447832	32.41852054333656	22.86218932671685
43	17.75684427435973	30.196391774254593	31.643887463728497	20.402876487657178
44	15.321922704907692	32.014382438285885	31.591740611463898	21.07195424534253
45	15.262626687413263	35.93675091467261	28.856554102359222	19.9440682955549
46	16.68068463770554	37.82370999621515	28.142478657639092	17.353126708440218
47	16.553261280962197	31.251524454350477	32.653601917658435	19.54161234702889
48	16.899364985911937	30.71870137516296	34.323562807519245	18.058370831405863
49	17.147062534168807	30.69262794903066	33.09222423146474	19.0680852853358
50	16.724841246477983	32.74948483956432	32.94209176163842	17.583582152319273
51	15.984692375625553	31.923545985953993	31.354556541486183	20.737205096934268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	387.0
1	403.0
2	419.0
3	2037.5
4	3656.0
5	2549.5
6	1443.0
7	1430.5
8	1418.0
9	1409.5
10	1401.0
11	1431.0
12	1461.0
13	1374.0
14	1287.0
15	1228.5
16	1170.0
17	1121.0
18	1072.0
19	1322.0
20	1572.0
21	1695.0
22	1818.0
23	2013.0
24	2208.0
25	3145.0
26	5055.5
27	6029.0
28	7498.0
29	8967.0
30	10438.5
31	11910.0
32	13284.5
33	14659.0
34	16003.5
35	17348.0
36	18124.5
37	18901.0
38	19820.0
39	20739.0
40	21845.5
41	22952.0
42	22265.0
43	21578.0
44	20668.5
45	19759.0
46	18230.0
47	16701.0
48	14057.5
49	11414.0
50	9489.5
51	7565.0
52	6126.5
53	4688.0
54	3900.5
55	3113.0
56	2717.5
57	2322.0
58	2128.5
59	1935.0
60	1702.5
61	1470.0
62	1213.5
63	957.0
64	776.5
65	596.0
66	462.5
67	329.0
68	279.5
69	230.0
70	164.5
71	99.0
72	75.0
73	51.0
74	42.5
75	26.5
76	19.0
77	15.5
78	12.0
79	10.0
80	8.0
81	6.0
82	4.0
83	4.0
84	4.0
85	3.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	237790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.78724925354304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21541067355108	21.61739349846503
2	8.559056946550287	5.270196391774254
3	3.942138261689137	3.6410277976365704
4	2.5010586130120616	3.0800285966609193
5	1.7880315261784754	2.7524286134824845
6	1.3850756054583453	2.558560074014887
7	1.1624253848570532	2.505151604356785
8	0.9862175415590979	2.4290340216157116
9	0.8359627914600664	2.316329534463182
>10	8.493491237416166	49.39063879894024
>50	0.10381237279569451	2.0518104209596704
>100	0.024587140925296073	1.486605828672358
>500	0.0013659522736275593	0.2897514613734808
>1k	0.0013659522736275593	0.6110433575844233
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1453	0.6110433575844233	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	689	0.2897514613734808	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	405	0.1703183481222928	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	316	0.132890365448505	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	314	0.13204928718617268	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAAGAGTCGTATGCCGTCTTC	312	0.13120820892384036	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	244	0.10261154800454184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.20539131166155E-4	0.0	0.0	0.029858278312797006	0.0
2	4.20539131166155E-4	0.0	0.0	0.13709575676016653	0.0
3	4.20539131166155E-4	0.0	0.0	0.17115942638462509	0.0
4	4.20539131166155E-4	0.0	0.0	0.24727700912569914	0.0
5	4.20539131166155E-4	0.0	0.0	0.5214685226460322	0.0
6	4.20539131166155E-4	0.0	0.0	0.6514151141763741	0.0
7	4.20539131166155E-4	0.0	0.0	0.7590731317549098	0.0
8	4.20539131166155E-4	0.0	0.0	1.0412548887673998	0.0
9	4.20539131166155E-4	0.0	0.0	1.1135876193279786	0.0
10	4.20539131166155E-4	0.0	0.0	1.402498002439127	0.0
11	4.20539131166155E-4	0.0	0.0	1.6203372723831952	0.0
12	4.20539131166155E-4	0.0	0.0	1.8798099163127129	0.0
13	4.20539131166155E-4	0.0	0.0	1.9672820555952732	0.0
14	4.20539131166155E-4	0.0	0.0	1.996719794776904	0.0
15	4.20539131166155E-4	0.0	0.0	2.0467639513856764	0.0
16	4.20539131166155E-4	0.0	0.0	2.143067412422726	0.0
17	4.20539131166155E-4	0.0	0.0	2.2519870473947603	0.0
18	4.20539131166155E-4	0.0	0.0	2.373943395432945	0.0
19	4.20539131166155E-4	0.0	0.0	2.4534252912233483	0.0
20	4.20539131166155E-4	0.0	0.0	2.5518314479162285	0.0
21	4.20539131166155E-4	0.0	0.0	2.6679002481180873	0.0
22	4.20539131166155E-4	0.0	0.0	2.81635056141974	0.0
23	4.20539131166155E-4	0.0	0.0	2.9673241095083895	0.0
24	4.20539131166155E-4	0.0	0.0	3.08213129231675	0.0
25	4.20539131166155E-4	0.0	0.0	3.1788552924849656	0.0
26	4.20539131166155E-4	0.0	0.0	3.28230791875184	0.0
27	4.20539131166155E-4	0.0	0.0	3.372303292821397	0.0
28	8.4107826233231E-4	0.0	0.0	3.4631397451532866	0.0
29	8.4107826233231E-4	0.0	0.0	3.56995668446949	0.0
30	0.001261617393498465	0.0	0.0	3.7091551368854874	0.0
31	0.001261617393498465	0.0	0.0	3.8374195718911643	0.0
32	0.001261617393498465	0.0	0.0	3.950124059043694	0.0
33	0.001261617393498465	0.0	0.0	4.061566928802725	0.0
34	0.001261617393498465	0.0	0.0	4.195718911644729	0.0
35	0.001261617393498465	0.0	0.0	4.3374405988477225	0.0
36	0.001261617393498465	0.0	0.0	4.4779006686572185	0.0
37	0.001261617393498465	0.0	0.0	4.624668825434207	0.0
38	0.001261617393498465	0.0	0.0	4.782370999621515	0.0
39	0.001261617393498465	0.0	0.0	4.935026704234829	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	7.0219894E-4	45.000004	44
GTTGAGC	20	7.0219894E-4	45.000004	42
CGGAACA	20	7.0219894E-4	45.000004	15
CCGGGAT	30	2.1582164E-6	45.000004	5
ACACGGG	45	3.8198777E-10	45.000004	3
GATCGTA	20	7.0219894E-4	45.000004	9
CGCGGCC	20	7.0219894E-4	45.000004	35
CGGCCCT	20	7.0219894E-4	45.000004	37
GACGTAC	20	7.0219894E-4	45.000004	9
TAGCGAA	20	7.0219894E-4	45.000004	1
AGGACGC	20	7.0219894E-4	45.000004	7
TATATCG	20	7.0219894E-4	45.000004	29
ACGGGAC	40	6.7757355E-9	45.000004	5
AGAACGC	20	7.0219894E-4	45.000004	10
ATCGTAC	20	7.0219894E-4	45.000004	10
AGGCCGA	20	7.0219894E-4	45.000004	7
GTTCGAA	20	7.0219894E-4	45.000004	1
CGAGGAT	20	7.0219894E-4	45.000004	5
GTGTCAT	20	7.0219894E-4	45.000004	29
CGGGCTG	20	7.0219894E-4	45.000004	6
>>END_MODULE