FastQCFastQC Report
Sat 18 Jun 2016
SRR3549374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565179
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15180.2685874740568917No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10470.18525104435939763No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.18330475831550713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8350.14774080424078034No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6390.11306152564054928No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6010.10633799203438202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.030238E-445.00000432
AGGTACT406.8048394E-945.0000047
GCGACTG207.030238E-445.00000443
CGTTGAT302.163535E-645.00000425
GGCCGAT302.163535E-645.0000048
GCAAACG207.030238E-445.00000410
TACGGAG302.163535E-645.00000440
AGGCGCT207.030238E-445.0000047
TCGGCCC207.030238E-445.00000441
CTGCGAA207.030238E-445.0000041
TACGCTA207.030238E-445.0000041
AAGCCGT207.030238E-445.00000437
TCTACGG207.030238E-445.00000438
GTTCGAA207.030238E-445.0000041
CGCTATC207.030238E-445.00000413
CGTAGCT207.030238E-445.00000415
GAGCGAT600.045.0000046
CGAGGCG207.030238E-445.0000045
CAGGCGA207.030238E-445.0000046
CTCGCAG207.030238E-445.0000042