FastQCFastQC Report
Sat 18 Jun 2016
SRR3549371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062583
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135031.2707713185699376No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG31260.2941887833703344No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC28930.27226108454586606No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC23090.21730067204161932No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13480.1268606781776106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.11688498686690829No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA406.8139343E-945.012
CGGGTAC950.045.06
TCGTTAA207.0330466E-445.025
CGAACCC253.8906066E-545.034
GCGACCG207.0330466E-445.01
CGCGGGT406.8139343E-945.04
CGCATAT351.2118107E-745.022
CAACCGG207.0330466E-445.02
CACGCCG207.0330466E-445.026
CGCGAGG700.045.02
ATGTCGC207.0330466E-445.042
TCGATAG207.0330466E-445.01
AAAGTCG207.0330466E-445.025
CCGTAGA253.8906066E-545.038
TACGCGG351.2118107E-745.02
TCGCTTG207.0330466E-445.014
TGCGACC207.0330466E-445.07
CGCTAAG253.8906066E-545.030
TCTCGAT253.8906066E-545.035
GATATCG502.1827873E-1145.09