##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062583
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91920631141285	33.0	31.0	34.0	30.0	34.0
2	32.23731134414911	33.0	31.0	34.0	31.0	34.0
3	32.1648718264832	33.0	31.0	34.0	30.0	34.0
4	35.95125463140291	37.0	35.0	37.0	35.0	37.0
5	32.122004586935795	37.0	35.0	37.0	0.0	37.0
6	34.02382496237941	37.0	35.0	37.0	19.0	37.0
7	35.454443558761994	37.0	35.0	37.0	32.0	37.0
8	35.69020208303728	37.0	35.0	37.0	35.0	37.0
9	37.92312506411264	39.0	38.0	39.0	35.0	39.0
10	37.58890834880663	39.0	37.0	39.0	35.0	39.0
11	37.48636953536806	39.0	37.0	39.0	35.0	39.0
12	37.401726735699704	39.0	37.0	39.0	35.0	39.0
13	37.39595871569562	39.0	37.0	39.0	35.0	39.0
14	38.76390079645543	40.0	38.0	41.0	35.0	41.0
15	38.82579337331766	40.0	38.0	41.0	35.0	41.0
16	38.80237684962022	40.0	38.0	41.0	35.0	41.0
17	38.68491214333375	40.0	38.0	41.0	35.0	41.0
18	38.607042461624175	40.0	38.0	41.0	35.0	41.0
19	38.504742688335874	40.0	37.0	41.0	35.0	41.0
20	38.42770400053455	40.0	37.0	41.0	34.0	41.0
21	38.38588703188363	40.0	37.0	41.0	34.0	41.0
22	38.248616813933594	40.0	36.0	41.0	34.0	41.0
23	38.21021793121102	40.0	36.0	41.0	34.0	41.0
24	38.176109536855	40.0	36.0	41.0	34.0	41.0
25	38.061006057879716	40.0	36.0	41.0	34.0	41.0
26	37.9946074800745	40.0	36.0	41.0	34.0	41.0
27	36.90460509908402	40.0	35.0	41.0	33.0	41.0
28	37.268875937220905	40.0	35.0	41.0	33.0	41.0
29	37.508846838317574	40.0	35.0	41.0	33.0	41.0
30	37.47374934475707	40.0	36.0	41.0	33.0	41.0
31	37.407439230629514	40.0	35.0	41.0	33.0	41.0
32	37.202775689052054	40.0	35.0	41.0	33.0	41.0
33	36.94806993900712	39.0	35.0	40.0	32.0	41.0
34	36.676776308297796	39.0	35.0	40.0	31.0	41.0
35	36.46518813118599	39.0	35.0	40.0	31.0	41.0
36	36.34136251003451	39.0	35.0	40.0	30.0	41.0
37	36.21918193684635	39.0	35.0	40.0	30.0	41.0
38	36.08223263500357	39.0	35.0	40.0	30.0	41.0
39	36.00813771724185	39.0	35.0	40.0	30.0	41.0
40	35.85484051598793	38.0	35.0	40.0	29.0	41.0
41	35.62398890251397	38.0	35.0	40.0	28.0	41.0
42	35.60617476470073	38.0	35.0	40.0	28.0	41.0
43	35.460018652660544	38.0	35.0	40.0	27.0	41.0
44	35.30092802162278	38.0	35.0	40.0	27.0	41.0
45	35.26713583785926	38.0	35.0	40.0	27.0	41.0
46	35.159940447005084	38.0	35.0	40.0	26.0	41.0
47	34.92896178463236	38.0	34.0	40.0	25.0	40.0
48	34.85545129180497	38.0	34.0	40.0	25.0	40.0
49	34.68183473667469	38.0	34.0	40.0	24.0	40.0
50	34.60970107746877	38.0	34.0	40.0	24.0	40.0
51	34.87046753053644	38.0	34.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	2.0
13	7.0
14	7.0
15	15.0
16	40.0
17	93.0
18	277.0
19	581.0
20	1148.0
21	1819.0
22	2805.0
23	4216.0
24	6311.0
25	9972.0
26	14662.0
27	16815.0
28	16402.0
29	15424.0
30	16823.0
31	19958.0
32	25626.0
33	34988.0
34	57257.0
35	89922.0
36	80820.0
37	120896.0
38	248941.0
39	276753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.93573207928228	24.461806748272842	28.688676555149108	16.91378461729578
2	30.739057560679967	27.1233400120273	30.51931002095836	11.618292406334376
3	26.081727262717358	25.85398034788812	36.045090124724375	12.019202264670149
4	24.796933510135208	26.570818467827923	35.680130399225284	12.952117622811581
5	19.828192244746997	39.21444254237081	29.47421519071922	11.483150022162974
6	20.3123897144976	40.281841512615955	29.70619706884074	9.699571704045708
7	84.4713307101657	3.369430905632784	10.744007762217162	1.4152306219843531
8	84.94875223864865	4.2679960059590645	9.25104203624564	1.5322097191466455
9	79.28001859619437	7.228800009034589	10.089847099003089	3.401334295767954
10	30.82516848095631	45.29537927860694	16.200334467989794	7.679117772446952
11	24.1688413987425	25.718932074012102	35.343497872636775	14.768728654608628
12	26.934742980077793	24.02033535262657	33.207382388011105	15.837539279284535
13	21.258386403697404	27.592762165402608	32.99243447335408	18.156416957545904
14	14.868579678011034	32.03298001191436	32.7231849182605	20.375255391814097
15	14.163223014108075	32.22317691888539	37.611273660504644	16.002326406501894
16	17.519384368091718	29.42631305036877	36.19679592088335	16.857506660656156
17	16.69347241580187	30.75157422996603	32.731560734549674	19.823392619682416
18	16.39231947057312	31.85332345802634	35.56983313303525	16.184523938365285
19	19.527886292176706	29.68746912005933	32.74595960974342	18.03868497802054
20	20.600743659554123	29.064176633731197	36.685793015698536	13.649286691016139
21	19.68156840453875	32.2378581249653	33.964311493784486	14.116261976711467
22	20.49082283454563	25.948843525635173	34.02040122983334	19.539932409985855
23	17.60144854566655	30.269541297009265	35.48889827900503	16.640111878319154
24	15.742581991242096	29.34716629195084	40.18895465107196	14.7212970657351
25	15.170297285012088	34.66788006207515	33.97005222180291	16.191770431109852
26	15.420630670733487	35.41332771181169	31.340704679069773	17.825336938385046
27	15.073834232243504	37.661622668535074	32.15654682975354	15.107996269467892
28	14.649396800061737	32.40659788458878	37.59743944708319	15.34656586826629
29	15.142064196396893	28.40154604393257	36.77877398753792	19.677615772132622
30	15.59689925398769	34.50271649367626	34.17427156278615	15.726112689549899
31	18.99484557912182	32.53119991567718	31.90254314251216	16.571411362688846
32	19.069851484542856	31.987148298062362	32.2572448458144	16.685755371580385
33	18.54057518330333	31.39321822389404	32.52640029061259	17.53980630219004
34	16.791347123001216	30.017137484789423	33.36238204450852	19.82913334770084
35	18.774062826150992	29.85169158550438	34.161284342023166	17.212961246321466
36	20.972102885139325	32.1467593590336	30.748468590218366	16.13266916560871
37	16.52595609001838	34.24259563723492	33.22018138818332	16.011266884563373
38	16.591739186491786	33.18818388775277	33.01473861336008	17.20533831239536
39	17.13324982613123	32.48574464300671	33.43343531752343	16.94757021333863
40	19.289975465445995	30.632807037191444	33.05031230501523	17.02690519234733
41	16.208145622506663	30.246578384935574	34.29962647623762	19.24564951632014
42	16.934959433757175	29.655753950514924	32.757817506961814	20.65146910876609
43	18.08169338301102	31.676866654181367	31.187399007889265	19.054040954918346
44	16.218874196180437	32.688175888377664	31.581627035252772	19.511322880189123
45	15.906522125800995	35.24279985657591	30.252130892363233	18.598547125259863
46	16.919337124723434	35.68812977433292	29.920768542316225	17.47176455862742
47	16.27910478522619	31.11502819073898	34.57856939175575	18.02729763227908
48	18.119149280573847	30.17176070010531	35.278561768821824	16.43052825049902
49	16.949264198655538	31.72843909605179	33.847520617212965	17.474776088079707
50	16.192429203177543	32.744359734721904	33.034125334209186	18.029085727891374
51	16.314207925404414	31.051974292831712	31.89943750276449	20.73438027899938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1497.0
1	1688.0
2	1879.0
3	12625.5
4	23372.0
5	16008.5
6	8645.0
7	8411.5
8	8178.0
9	8265.5
10	8353.0
11	8347.0
12	8341.0
13	8110.5
14	7880.0
15	7377.0
16	6874.0
17	6638.5
18	6403.0
19	7369.5
20	8336.0
21	8243.0
22	8150.0
23	10064.0
24	11978.0
25	13558.5
26	20774.0
27	26409.0
28	29913.5
29	33418.0
30	40233.5
31	47049.0
32	55759.0
33	64469.0
34	69227.5
35	73986.0
36	76213.0
37	78440.0
38	83229.0
39	88018.0
40	89731.5
41	91445.0
42	92287.0
43	93129.0
44	89078.5
45	85028.0
46	82896.0
47	80764.0
48	69029.0
49	57294.0
50	50086.0
51	42878.0
52	33902.0
53	24926.0
54	20517.5
55	16109.0
56	13204.5
57	10300.0
58	9456.0
59	8612.0
60	7560.5
61	6509.0
62	5330.0
63	4151.0
64	3130.0
65	2109.0
66	1559.0
67	1009.0
68	843.5
69	678.0
70	521.5
71	365.0
72	298.5
73	232.0
74	145.5
75	61.5
76	64.0
77	46.0
78	28.0
79	31.5
80	35.0
81	23.5
82	12.0
83	17.0
84	22.0
85	14.5
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1062583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.099431470599818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30101080454675	17.856094016877236
2	8.330144729492973	3.848432146382024
3	3.2073651193898085	2.2226493232961126
4	1.71378083367945	1.583494516928235
5	1.1503854487346197	1.3286624918910284
6	0.7852777577666525	1.0883681850550246
7	0.6441892921306134	1.0416284485365717
8	0.48619126496020226	0.8984593445241945
9	0.411437485174806	0.8553574793908223
>10	4.127642633245084	20.262447454650125
>50	0.9945715338297366	16.59238154216566
>100	0.8408840587037207	29.323770606045674
>500	0.0037689026535525335	0.5341584514405269
>1k	0.0029313687305408593	1.2579131659783795
>5k	0.0	0.0
>10k+	4.187669615058371E-4	1.306182826838362
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13503	1.2707713185699376	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	3126	0.2941887833703344	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC	2893	0.27226108454586606	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	2309	0.21730067204161932	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1348	0.1268606781776106	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1242	0.11688498686690829	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8233088615195234E-4	0.0	0.0	0.06088936111343773	0.0
2	2.8233088615195234E-4	0.0	0.0	0.23725205466302396	0.0
3	2.8233088615195234E-4	0.0	0.0	0.31555182042249874	0.0
4	2.8233088615195234E-4	0.0	0.0	0.4526705207969636	0.0
5	2.8233088615195234E-4	0.0	0.0	0.9229396668307323	0.0
6	2.8233088615195234E-4	0.0	0.0	1.1197242944786432	0.0
7	2.8233088615195234E-4	0.0	0.0	1.315661929468098	0.0
8	2.8233088615195234E-4	0.0	0.0	1.582558727177077	0.0
9	2.8233088615195234E-4	0.0	0.0	1.6675403239088147	0.0
10	2.8233088615195234E-4	0.0	0.0	2.2131918165451547	0.0
11	2.8233088615195234E-4	0.0	0.0	2.5777751008627092	0.0
12	2.8233088615195234E-4	0.0	0.0	3.1098747109637555	0.0
13	2.8233088615195234E-4	0.0	0.0	3.235982506778294	0.0
14	2.8233088615195234E-4	0.0	0.0	3.2925427943040684	0.0
15	2.8233088615195234E-4	0.0	0.0	3.391452714752636	0.0
16	2.8233088615195234E-4	0.0	0.0	3.554357636062312	0.0
17	2.8233088615195234E-4	0.0	0.0	3.7379668223564653	0.0
18	2.8233088615195234E-4	0.0	0.0	3.942186163339711	0.0
19	2.8233088615195234E-4	0.0	0.0	4.130312643812295	0.0
20	3.764411815359365E-4	0.0	0.0	4.26790189566368	0.0
21	3.764411815359365E-4	0.0	0.0	4.440688397988675	0.0
22	3.764411815359365E-4	0.0	0.0	4.654695209691854	0.0
23	3.764411815359365E-4	0.0	0.0	4.881406911271872	0.0
24	3.764411815359365E-4	0.0	0.0	5.042523736969253	0.0
25	3.764411815359365E-4	0.0	0.0	5.173525268143759	0.0
26	3.764411815359365E-4	0.0	0.0	5.2951157697798665	0.0
27	3.764411815359365E-4	0.0	0.0	5.440798507034274	0.0
28	3.764411815359365E-4	0.0	0.0	5.5905279869901925	0.0
29	3.764411815359365E-4	0.0	0.0	5.754750452435245	0.0
30	3.764411815359365E-4	0.0	0.0	5.950970418310852	0.0
31	3.764411815359365E-4	0.0	0.0	6.123851030931231	0.0
32	3.764411815359365E-4	0.0	0.0	6.2954140994162335	0.0
33	3.764411815359365E-4	0.0	0.0	6.472906116510428	0.0
34	3.764411815359365E-4	0.0	0.0	6.638257905500088	0.0
35	3.764411815359365E-4	0.0	0.0	6.832595665468015	0.0
36	3.764411815359365E-4	0.0	0.0	7.02900385193439	0.0
37	3.764411815359365E-4	0.0	0.0	7.229647001693044	0.0
38	3.764411815359365E-4	0.0	0.0	7.4248317543194275	0.0
39	5.646617723039047E-4	0.0	0.0	7.619169514287354	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	40	6.8139343E-9	45.0	12
CGGGTAC	95	0.0	45.0	6
TCGTTAA	20	7.0330466E-4	45.0	25
CGAACCC	25	3.8906066E-5	45.0	34
GCGACCG	20	7.0330466E-4	45.0	1
CGCGGGT	40	6.8139343E-9	45.0	4
CGCATAT	35	1.2118107E-7	45.0	22
CAACCGG	20	7.0330466E-4	45.0	2
CACGCCG	20	7.0330466E-4	45.0	26
CGCGAGG	70	0.0	45.0	2
ATGTCGC	20	7.0330466E-4	45.0	42
TCGATAG	20	7.0330466E-4	45.0	1
AAAGTCG	20	7.0330466E-4	45.0	25
CCGTAGA	25	3.8906066E-5	45.0	38
TACGCGG	35	1.2118107E-7	45.0	2
TCGCTTG	20	7.0330466E-4	45.0	14
TGCGACC	20	7.0330466E-4	45.0	7
CGCTAAG	25	3.8906066E-5	45.0	30
TCTCGAT	25	3.8906066E-5	45.0	35
GATATCG	50	2.1827873E-11	45.0	9
>>END_MODULE