FastQCFastQC Report
Sat 18 Jun 2016
SRR3549367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123041
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20821.6921188872002015No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCG2440.19830788111279982No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTC2050.16661112962345886No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2050.16661112962345886No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1990.16173470631740638No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1820.14791817361692444No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1730.14060353865784575No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC1710.13897806422249495No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA1390.11297047325688186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.10321762664477696No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA207.008717E-445.0000045
GTGCAGG406.730261E-945.0000042
TCCGCCC207.008717E-445.00000435
CTCGTCC406.730261E-945.00000437
GGGCTCT207.008717E-445.0000047
GTCGAAG207.008717E-445.0000041
TTGTGAG207.008717E-445.0000041
GGATGTC207.008717E-445.0000048
GATCCGG207.008717E-445.0000049
GATTTTC207.008717E-445.0000049
TCCTCGT406.730261E-945.00000435
TTCCGCC207.008717E-445.00000434
CCTCGTC406.730261E-945.00000436
GGATACG207.008717E-445.0000048
CCTCGGG207.008717E-445.0000043
GTCAGGG207.008717E-445.0000043
GTCAGCG207.008717E-445.0000041
AGCGGGT207.008717E-445.0000044
AAACGCG207.008717E-445.0000041
GTCCGGG207.008717E-445.0000043