##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549367_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	123041
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42108727984981	33.0	31.0	34.0	31.0	34.0
2	32.75556928178412	34.0	31.0	34.0	31.0	34.0
3	32.7290740484879	34.0	31.0	34.0	31.0	34.0
4	36.281930413439426	37.0	37.0	37.0	35.0	37.0
5	36.3309547224096	37.0	37.0	37.0	35.0	37.0
6	36.3500459196528	37.0	37.0	37.0	35.0	37.0
7	36.374582456254416	37.0	37.0	37.0	35.0	37.0
8	36.17976121780545	37.0	37.0	37.0	35.0	37.0
9	38.12590924976227	39.0	39.0	39.0	37.0	39.0
10	37.89541697482953	39.0	38.0	39.0	35.0	39.0
11	37.95895677050739	39.0	38.0	39.0	35.0	39.0
12	37.81678464901943	39.0	38.0	39.0	35.0	39.0
13	37.81336302533302	39.0	38.0	39.0	35.0	39.0
14	39.261790785185426	41.0	39.0	41.0	35.0	41.0
15	39.23514113181785	41.0	39.0	41.0	35.0	41.0
16	39.221487146560904	41.0	39.0	41.0	35.0	41.0
17	39.104274185027755	41.0	39.0	41.0	35.0	41.0
18	38.99027153550443	40.0	38.0	41.0	35.0	41.0
19	38.920132313619035	40.0	38.0	41.0	35.0	41.0
20	38.82723644963874	40.0	38.0	41.0	35.0	41.0
21	38.794694451443014	40.0	38.0	41.0	35.0	41.0
22	38.798498061621736	40.0	38.0	41.0	35.0	41.0
23	38.77227915897953	40.0	38.0	41.0	35.0	41.0
24	38.73383668858348	40.0	38.0	41.0	35.0	41.0
25	38.634950951308916	40.0	37.0	41.0	35.0	41.0
26	38.60564364723954	40.0	37.0	41.0	35.0	41.0
27	38.5874058240749	40.0	37.0	41.0	35.0	41.0
28	38.456481985679574	40.0	37.0	41.0	35.0	41.0
29	38.41128566900464	40.0	37.0	41.0	35.0	41.0
30	38.25439487650458	40.0	37.0	41.0	35.0	41.0
31	38.096520671971135	40.0	37.0	41.0	35.0	41.0
32	37.84987930852318	40.0	36.0	41.0	34.0	41.0
33	37.58560154745166	40.0	36.0	41.0	34.0	41.0
34	37.290740484879024	40.0	36.0	41.0	33.0	41.0
35	37.0627920774376	40.0	36.0	41.0	33.0	41.0
36	36.96224835623898	40.0	36.0	41.0	32.0	41.0
37	36.86918994481514	40.0	36.0	41.0	32.0	41.0
38	36.78972862704302	40.0	36.0	41.0	31.0	41.0
39	36.67602669029023	40.0	36.0	41.0	31.0	41.0
40	36.571630594679824	40.0	35.0	41.0	31.0	41.0
41	36.407945319039996	40.0	35.0	40.0	30.0	41.0
42	36.356913549142156	40.0	35.0	40.0	30.0	41.0
43	36.26737428987086	39.0	35.0	40.0	30.0	41.0
44	36.18841686917369	39.0	35.0	40.0	30.0	41.0
45	36.10698872733479	39.0	35.0	40.0	30.0	41.0
46	35.978503100592484	39.0	35.0	40.0	29.0	41.0
47	35.89153209092904	39.0	35.0	40.0	29.0	41.0
48	35.80318755536772	38.0	35.0	40.0	29.0	41.0
49	35.64862119131021	38.0	35.0	40.0	28.0	41.0
50	35.55227119415479	38.0	35.0	40.0	28.0	41.0
51	35.488975219642235	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	5.0
16	4.0
17	10.0
18	25.0
19	31.0
20	85.0
21	127.0
22	196.0
23	339.0
24	532.0
25	1031.0
26	1563.0
27	1898.0
28	1809.0
29	1494.0
30	1356.0
31	1554.0
32	1803.0
33	2310.0
34	4351.0
35	9040.0
36	6056.0
37	8500.0
38	22954.0
39	55964.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.22732259978381	23.761185295958256	28.935883160897585	17.075608943360344
2	32.48917027657448	26.088051950162956	29.73886753196089	11.68391024130168
3	27.064149348591123	24.998984078477907	35.98068936370804	11.956177209222941
4	25.141213091571103	26.071797205809442	35.785632431465935	13.001357271153518
5	22.72575808063979	31.87961736331792	32.15513528010988	13.239489275932412
6	20.345250770068514	40.68725059126633	29.042351736413064	9.925146902252095
7	82.53915361546152	3.971846782779724	11.62539316162905	1.8636064401297128
8	82.26119748701652	4.9178729041539	10.94025568712868	1.8806739217008965
9	75.179005372193	8.867775782056388	12.326785380482928	3.6264334652676746
10	25.46386976698824	47.02172446582846	18.24676327403061	9.267642493152689
11	27.73221934152031	24.183808649149473	33.19625165595208	14.887720353378143
12	27.99148251395876	23.05816760266903	32.80451231703253	16.145837566339676
13	21.72690404011671	27.788298209539906	33.18324786046927	17.301549889874106
14	15.535471915865443	31.278191822238117	33.875699969928725	19.310636291967718
15	15.016945570988533	30.519095260929284	38.465227038141755	15.998732129940427
16	18.922960639136548	28.93669589811526	36.01644980128575	16.12389366146244
17	17.287733357173625	29.72505099926041	33.69445957038711	19.29275607317886
18	17.104054745978985	30.209442380994954	36.33910647670289	16.347396396323177
19	20.525678432392453	29.151258523581568	33.37911752992905	16.943945514096928
20	21.20431400915142	29.14394388862249	35.533683893986556	14.118058208239532
21	19.764143659430598	29.589323883908612	35.70273323526304	14.943799221397747
22	19.80234230866134	26.2319064376915	34.742890581188384	19.222860672458776
23	17.52261441308182	29.327622499817135	35.57025706878195	17.579506018319098
24	15.284336115603741	28.96595443795158	39.57461334026869	16.17509610617599
25	15.928023992002666	32.821579798603715	34.750205216147464	16.500190993246154
26	16.132021033639194	34.05287668338196	32.81914158695069	16.995960696028153
27	16.458741395144706	33.92040051690087	33.4433237701254	16.177534317829018
28	14.786128201168717	32.01696995310506	37.223364569533736	15.97353727619249
29	15.860566802935608	27.91914890158565	37.50782259572013	18.712461699758617
30	15.75734917629083	32.698043741517054	34.9208800318593	16.623727050332814
31	19.194414869840134	31.070131094513208	32.988190928227176	16.747263107419478
32	19.029429214652026	30.75560179127283	33.848879641745434	16.36608935232971
33	18.78804626100243	30.78973675441519	32.53874724685267	17.883469737729698
34	17.10811843206736	29.32680976259946	33.55304329451159	20.012028510821597
35	18.946530018449135	28.605099113303694	33.7741078177193	18.674263050527873
36	20.169699531050625	31.727635503612618	31.32207963199259	16.78058533334417
37	16.162092310693183	33.59286741817768	33.54897960842321	16.69606066270593
38	16.44411212522655	32.677725311075164	32.76550093058411	18.112661633114165
39	17.448655326273357	31.60491218374363	32.92317195081315	18.02326053916987
40	18.970912134979397	30.120854024268333	32.69398005542868	18.214253785323592
41	16.918750660348987	30.03470387919474	32.845961915133984	20.20058354532229
42	16.59609398493185	29.861590851829877	33.01501125641047	20.527303906827804
43	17.91679196365439	30.10703749156785	32.67366162498679	19.302508919790963
44	16.429482855308393	31.527702148064467	32.24941279736023	19.79340219926691
45	15.8443120585821	33.76191675945417	31.16765956063426	19.226111621329476
46	16.75213953072553	33.999236027015385	31.40010240488943	17.84852203736966
47	17.372258027811867	30.09159548443202	33.65626092115636	18.879885566599754
48	17.130875074162272	29.867280012353604	35.278484407636476	17.723360505847644
49	17.500670508204582	30.03064019310636	33.96347558943767	18.50521370925139
50	16.466056030103786	31.22942758917759	33.53028665241668	18.774229728301947
51	17.294235254915026	29.1171235604392	32.85977844783446	20.72886273681131
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	212.0
2	264.0
3	1718.5
4	3173.0
5	2123.5
6	1074.0
7	1048.0
8	1022.0
9	990.5
10	959.0
11	982.5
12	1006.0
13	987.5
14	969.0
15	917.5
16	866.0
17	855.5
18	845.0
19	787.0
20	729.0
21	870.0
22	1011.0
23	1103.5
24	1196.0
25	1522.5
26	2308.5
27	2768.0
28	3490.5
29	4213.0
30	4826.0
31	5439.0
32	5896.0
33	6353.0
34	6952.5
35	7552.0
36	7933.0
37	8314.0
38	8452.5
39	8591.0
40	9283.5
41	9976.0
42	10108.0
43	10240.0
44	10055.0
45	9870.0
46	9702.0
47	9534.0
48	8634.5
49	7735.0
50	6710.5
51	5686.0
52	4727.0
53	3768.0
54	2975.5
55	2183.0
56	1892.5
57	1602.0
58	1475.5
59	1349.0
60	1183.0
61	1017.0
62	861.0
63	705.0
64	548.0
65	391.0
66	328.5
67	266.0
68	216.0
69	166.0
70	124.5
71	83.0
72	69.5
73	56.0
74	45.0
75	22.5
76	11.0
77	11.0
78	11.0
79	8.0
80	5.0
81	2.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	123041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.326127063336614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4747687196135	39.18124852691379
2	10.516208678158291	11.215773603920644
3	5.1377013701553045	8.219211482351412
4	3.09542316309268	6.602677156395023
5	2.2251687927697255	5.932981689030486
6	1.61553350707939	5.169008704415601
7	1.2131742185237682	4.528571776887379
8	0.8672061938945026	3.699579814858462
9	0.5059972871229786	2.428458806414122
>10	1.3107158642342218	9.040076072203576
>50	0.018289058570710073	0.6721336790175633
>100	0.018289058570710073	1.6181598003917392
>500	0.0	0.0
>1k	0.0015240882142258394	1.6921188872002015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2082	1.6921188872002015	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCG	244	0.19830788111279982	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTC	205	0.16661112962345886	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	205	0.16661112962345886	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	199	0.16173470631740638	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	182	0.14791817361692444	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	173	0.14060353865784575	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC	171	0.13897806422249495	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	139	0.11297047325688186	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	127	0.10321762664477696	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07802277289683926	0.0
2	0.0	0.0	0.0	0.31371656602270787	0.0
3	0.0	0.0	0.0	0.4421290464154225	0.0
4	0.0	0.0	0.0	0.6209312343040125	0.0
5	0.0	0.0	0.0	1.0890678716850481	0.0
6	0.0	0.0	0.0	1.3905933794426248	0.0
7	0.0	0.0	0.0	1.6222234864801164	0.0
8	0.0	0.0	0.0	1.983078811127998	0.0
9	0.0	0.0	0.0	2.1196186636974668	0.0
10	0.0	0.0	0.0	2.676343657805122	0.0
11	0.0	0.0	0.0	3.224941279736023	0.0
12	0.0	0.0	0.0	3.7719134272315733	0.0
13	0.0	0.0	0.0	3.9531538267731894	0.0
14	0.0	0.0	0.0	4.023049227493274	0.0
15	0.0	0.0	0.0	4.150648970668314	0.0
16	0.0	0.0	0.0	4.3741517055290515	0.0
17	0.0	0.0	0.0	4.628538454661454	0.0
18	0.0	0.0	0.0	4.896741736494339	0.0
19	0.0	0.0	0.0	5.1397501645792865	0.0
20	0.0	0.0	0.0	5.351061841174893	0.0
21	0.0	0.0	0.0	5.577002787688657	0.0
22	0.0	0.0	0.0	5.836265960127112	0.0
23	0.0	0.0	0.0	6.10934566526605	0.0
24	0.0	0.0	0.0	6.323908290732358	0.0
25	0.0	0.0	0.0	6.522216171845157	0.0
26	0.0	0.0	0.0	6.711583943563528	0.0
27	0.0	0.0	0.0	6.909079087458652	0.0
28	0.0	0.0	0.0	7.100885070830048	0.0
29	0.0	0.0	0.0	7.326826017343812	0.0
30	0.0	0.0	0.0	7.569021708211084	0.0
31	0.0	0.0	0.0	7.782771596459717	0.0
32	0.0	0.0	0.0	8.011150754626506	0.0
33	0.0	0.0	0.0	8.232215277834218	0.0
34	0.0	0.0	0.0	8.461407173218683	0.0
35	0.0	0.0	0.0	8.69385001747385	0.0
36	0.0	0.0	0.0	8.960427824871385	0.0
37	0.0	0.0	0.0	9.21969099730984	0.0
38	0.0	0.0	0.0	9.510650921237636	0.0
39	0.0	0.0	0.0	9.791045261335652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	20	7.008717E-4	45.000004	5
GTGCAGG	40	6.730261E-9	45.000004	2
TCCGCCC	20	7.008717E-4	45.000004	35
CTCGTCC	40	6.730261E-9	45.000004	37
GGGCTCT	20	7.008717E-4	45.000004	7
GTCGAAG	20	7.008717E-4	45.000004	1
TTGTGAG	20	7.008717E-4	45.000004	1
GGATGTC	20	7.008717E-4	45.000004	8
GATCCGG	20	7.008717E-4	45.000004	9
GATTTTC	20	7.008717E-4	45.000004	9
TCCTCGT	40	6.730261E-9	45.000004	35
TTCCGCC	20	7.008717E-4	45.000004	34
CCTCGTC	40	6.730261E-9	45.000004	36
GGATACG	20	7.008717E-4	45.000004	8
CCTCGGG	20	7.008717E-4	45.000004	3
GTCAGGG	20	7.008717E-4	45.000004	3
GTCAGCG	20	7.008717E-4	45.000004	1
AGCGGGT	20	7.008717E-4	45.000004	4
AAACGCG	20	7.008717E-4	45.000004	1
GTCCGGG	20	7.008717E-4	45.000004	3
>>END_MODULE