##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129798
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9935129971186	33.0	31.0	34.0	30.0	34.0
2	32.26428758532489	33.0	31.0	34.0	30.0	34.0
3	32.025932602967686	33.0	31.0	34.0	30.0	34.0
4	35.8195503782801	37.0	35.0	37.0	35.0	37.0
5	30.88228632182314	37.0	35.0	37.0	0.0	37.0
6	33.315536448943746	37.0	35.0	37.0	17.0	37.0
7	35.4802385244765	37.0	35.0	37.0	32.0	37.0
8	35.844173253825176	37.0	35.0	37.0	35.0	37.0
9	37.803787423535034	39.0	38.0	39.0	35.0	39.0
10	37.482911909274414	39.0	37.0	39.0	34.0	39.0
11	37.5084901154101	39.0	37.0	39.0	35.0	39.0
12	37.3304673415615	39.0	37.0	39.0	34.0	39.0
13	37.24155225812416	39.0	37.0	39.0	34.0	39.0
14	38.596588545278046	40.0	38.0	41.0	34.0	41.0
15	38.65782985870352	40.0	38.0	41.0	34.0	41.0
16	38.685318726020434	40.0	38.0	41.0	35.0	41.0
17	38.575109015547234	40.0	38.0	41.0	34.0	41.0
18	38.42714833818703	40.0	38.0	41.0	34.0	41.0
19	38.336415044915945	40.0	37.0	41.0	34.0	41.0
20	38.208516309958554	40.0	36.0	41.0	34.0	41.0
21	38.14729810936994	40.0	36.0	41.0	34.0	41.0
22	38.19214471717592	40.0	36.0	41.0	34.0	41.0
23	38.20612027920307	40.0	36.0	41.0	34.0	41.0
24	38.20284596064654	40.0	36.0	41.0	34.0	41.0
25	38.13692814989445	40.0	36.0	41.0	34.0	41.0
26	38.07423843202515	40.0	36.0	41.0	34.0	41.0
27	37.567566526448786	40.0	35.0	41.0	33.0	41.0
28	37.61118044962172	40.0	36.0	41.0	33.0	41.0
29	37.73152899120171	40.0	36.0	41.0	33.0	41.0
30	37.62560285982835	40.0	36.0	41.0	33.0	41.0
31	37.52690334211621	40.0	35.0	41.0	33.0	41.0
32	37.326576680688454	40.0	35.0	41.0	33.0	41.0
33	37.109030955792846	40.0	35.0	41.0	32.0	41.0
34	36.879073637498266	40.0	35.0	41.0	31.0	41.0
35	36.66980230820197	40.0	35.0	41.0	30.0	41.0
36	36.53166458651135	40.0	35.0	41.0	30.0	41.0
37	36.45336599947611	40.0	35.0	41.0	30.0	41.0
38	36.38227861754418	40.0	35.0	41.0	30.0	41.0
39	36.27478081326369	40.0	35.0	41.0	30.0	41.0
40	36.139277954976194	40.0	35.0	41.0	29.0	41.0
41	36.049330498158675	39.0	35.0	41.0	28.0	41.0
42	35.976840937456664	39.0	35.0	41.0	27.0	41.0
43	35.91443627790875	39.0	35.0	40.0	27.0	41.0
44	35.792284935052926	39.0	35.0	40.0	27.0	41.0
45	35.739587666990246	39.0	35.0	40.0	27.0	41.0
46	35.60752091711737	39.0	35.0	40.0	26.0	41.0
47	35.516810736683155	39.0	35.0	40.0	26.0	41.0
48	35.446771136689314	38.0	35.0	40.0	26.0	41.0
49	35.38673169078106	38.0	35.0	40.0	26.0	41.0
50	35.284025948011525	38.0	35.0	40.0	25.0	41.0
51	35.29013544122406	38.0	35.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	1.0
16	7.0
17	11.0
18	45.0
19	83.0
20	147.0
21	237.0
22	354.0
23	543.0
24	803.0
25	1293.0
26	1872.0
27	2122.0
28	1981.0
29	1980.0
30	2123.0
31	2534.0
32	3206.0
33	4301.0
34	6377.0
35	10538.0
36	9026.0
37	13118.0
38	22320.0
39	44767.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.355614108075624	24.48573937965146	29.621411732075998	16.537234780196922
2	31.52899120171343	26.111342239479807	30.220804634894222	12.138861923912541
3	27.428003513151207	25.14599608622629	35.267107351422986	12.158893049199525
4	25.176042774156766	26.981925761567975	34.675418727561286	13.16661273671397
5	19.2021448712615	41.6585771737623	27.41721752261206	11.722060432364136
6	20.545771121280758	40.796468358526326	28.74081264734433	9.916947872848581
7	81.86643862000955	4.798224934128415	11.044854312084931	2.2904821337770995
8	81.37182391100018	5.660333749364397	10.488605371423288	2.4792369682121453
9	74.68373934883434	9.669640518343888	11.721290004468482	3.9253301283532873
10	24.93721012650426	48.169463319927885	17.566526448789656	9.326800104778194
11	27.68070386292547	24.90793386646944	32.40034515169725	15.01101711890784
12	28.179941139308774	23.82394181728532	31.738547589331112	16.257569454074794
13	21.697560825282363	28.766236767900892	32.05827516602721	17.477927240789533
14	15.698238801830536	31.445014561087227	33.35875745388989	19.497989183192345
15	15.668192113900059	31.16380837917379	37.22707591796484	15.940923588961308
16	19.009537897348185	29.76008875329358	35.194687129231575	16.035686220126657
17	17.736791013729025	30.29476571287693	32.790181666897794	19.178261606496246
18	17.63124239202453	30.68845436755574	35.482056734310234	16.19824650610949
19	20.7098722630549	29.576726914128106	32.63224394828888	17.081156874528112
20	21.361654262777545	29.694602382163055	34.60608021695249	14.337663138106905
21	20.147459899228032	30.164563398511536	34.53751213423936	15.15046456802108
22	20.288448204132575	26.52583244734125	34.176951879073634	19.008767469452533
23	17.8492734864944	29.661473982649966	34.84568329250066	17.643569238354985
24	15.453242731012804	29.440361176597484	38.88195503782801	16.2244410545617
25	16.348479945761877	33.01437618453289	34.196212576464966	16.440931293240265
26	16.554954621796945	34.54521641319589	31.983543660148843	16.91628530485832
27	16.53030092913604	35.31564430884914	32.251652567836175	15.902402194178647
28	15.0133284025948	32.656897640949786	36.32182314057227	16.00795081588314
29	15.911647328926486	28.626018890892002	36.656959275181435	18.805374505000078
30	16.111188153900677	32.868765312254425	34.29405691921293	16.725989614631967
31	19.654386046009954	31.328679948843586	32.49433735496695	16.522596650179512
32	19.241436693939814	30.888765620425584	33.627636789472874	16.242160896161728
33	19.3801137151574	30.98429867948659	32.09294442133161	17.542643184024406
34	17.26991170896316	30.05670349312008	32.988181636080675	19.685203161836085
35	18.97486864204379	28.8633106827532	33.84720873973405	18.31461193546896
36	20.167491024515016	31.764742137783326	31.28553598668701	16.782230851014653
37	16.48176397170989	34.04135656943867	32.92423612074146	16.552643338109988
38	16.769133576788548	33.362609593368155	32.0436370360098	17.824619793833495
39	17.5757715835375	32.195411331453485	32.44580039754079	17.78301668746822
40	19.22602813602675	30.74469560393843	32.08292885868811	17.946347401346706
41	17.122759980893388	30.384905776668365	32.68771475677592	19.804619485662336
42	16.85927364057998	30.591380452703433	32.27168369312316	20.27766221359343
43	18.05574816252947	30.409559469329267	32.34410391531456	19.190588452826702
44	16.407032465831524	32.16151250404475	31.881847177922616	19.549607852201113
45	16.185919659779042	34.53905299003066	30.516648946825065	18.758378403365228
46	16.82614524106689	34.75092066133531	30.726975762338405	17.695958335259405
47	17.64279881045933	30.638376554338283	32.90728670703709	18.811537928165304
48	17.41552258124162	30.5952325921817	34.41963666620441	17.569608160372272
49	17.593491425137522	30.89030647621689	33.24242284164625	18.273779256999337
50	16.670518806144933	32.158430792462134	32.75474198369774	18.416308417695188
51	17.527234626111344	30.03513151204179	32.40650857486248	20.03112528698439
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151.0
1	214.5
2	278.0
3	1541.5
4	2805.0
5	1973.0
6	1141.0
7	1049.0
8	957.0
9	988.5
10	1020.0
11	999.5
12	979.0
13	974.5
14	970.0
15	907.5
16	845.0
17	839.5
18	834.0
19	810.0
20	786.0
21	908.5
22	1031.0
23	1182.0
24	1333.0
25	1715.5
26	2551.0
27	3004.0
28	3795.0
29	4586.0
30	5280.5
31	5975.0
32	6464.0
33	6953.0
34	7617.5
35	8282.0
36	8683.0
37	9084.0
38	9386.5
39	9689.0
40	10429.5
41	11170.0
42	11200.5
43	11231.0
44	10855.5
45	10480.0
46	10073.5
47	9667.0
48	8510.0
49	7353.0
50	6506.5
51	5660.0
52	4575.0
53	3490.0
54	2803.0
55	2116.0
56	1900.5
57	1685.0
58	1525.5
59	1366.0
60	1207.5
61	1049.0
62	876.0
63	703.0
64	544.5
65	386.0
66	324.5
67	263.0
68	208.5
69	154.0
70	126.0
71	98.0
72	79.0
73	60.0
74	47.0
75	24.0
76	14.0
77	11.5
78	9.0
79	7.0
80	5.0
81	4.0
82	3.0
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	129798.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.52167213670472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92775748323427	42.35042142405892
2	10.773676462570197	12.178924174486509
3	4.883866746633226	8.28132945037674
4	2.754757101575705	6.228139108460839
5	1.9423695545499153	5.489298756529377
6	1.3685186194863965	4.641057643415153
7	1.0277520309688675	4.066318433257832
8	0.6733547789106373	3.0447310436216273
9	0.5534049397524671	2.81514353071696
>10	1.0645548225287607	7.813679717715219
>50	0.013630663540701162	0.5131049785050616
>100	0.014993729894771277	1.3836885005932296
>500	0.0	0.0
>1k	0.0013630663540701161	1.1941632382625311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	1.1941632382625311	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	227	0.1748871323132868	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	202	0.15562643492195566	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	193	0.14869258386107645	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	190	0.1463813001741167	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	189	0.14561087227846348	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCG	164	0.12635017488713232	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTC	160	0.12326846330451932	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.704278956532459E-4	0.0	0.0	0.05778209217399344	0.0
2	7.704278956532459E-4	0.0	0.0	0.18721397864373873	0.0
3	7.704278956532459E-4	0.0	0.0	0.2534707776699179	0.0
4	7.704278956532459E-4	0.0	0.0	0.366723678330945	0.0
5	7.704278956532459E-4	0.0	0.0	0.6633384181574447	0.0
6	7.704278956532459E-4	0.0	0.0	0.833602983096812	0.0
7	7.704278956532459E-4	0.0	0.0	0.9923111296013806	0.0
8	7.704278956532459E-4	0.0	0.0	1.2480931909582582	0.0
9	7.704278956532459E-4	0.0	0.0	1.3374628268540347	0.0
10	7.704278956532459E-4	0.0	0.0	1.6926300867501811	0.0
11	7.704278956532459E-4	0.0	0.0	2.0223732260897704	0.0
12	7.704278956532459E-4	0.0	0.0	2.366754495446771	0.0
13	7.704278956532459E-4	0.0	0.0	2.4838595355860646	0.0
14	7.704278956532459E-4	0.0	0.0	2.5370190603861387	0.0
15	7.704278956532459E-4	0.0	0.0	2.632552119447141	0.0
16	7.704278956532459E-4	0.0	0.0	2.7812447033082175	0.0
17	7.704278956532459E-4	0.0	0.0	2.949968412456278	0.0
18	7.704278956532459E-4	0.0	0.0	3.141034530578283	0.0
19	7.704278956532459E-4	0.0	0.0	3.272007272839335	0.0
20	7.704278956532459E-4	0.0	0.0	3.4230111403873713	0.0
21	7.704278956532459E-4	0.0	0.0	3.587882710057166	0.0
22	7.704278956532459E-4	0.0	0.0	3.773555832909598	0.0
23	7.704278956532459E-4	0.0	0.0	3.968474090509869	0.0
24	7.704278956532459E-4	0.0	0.0	4.124100525431825	0.0
25	7.704278956532459E-4	0.0	0.0	4.262007118753756	0.0
26	7.704278956532459E-4	0.0	0.0	4.378341730997396	0.0
27	7.704278956532459E-4	0.0	0.0	4.487742492180157	0.0
28	7.704278956532459E-4	0.0	0.0	4.624108229710782	0.0
29	7.704278956532459E-4	0.0	0.0	4.795913650441455	0.0
30	7.704278956532459E-4	0.0	0.0	4.9838980569808475	0.0
31	7.704278956532459E-4	0.0	0.0	5.140294919798456	0.0
32	7.704278956532459E-4	0.0	0.0	5.2866762199725725	0.0
33	7.704278956532459E-4	0.0	0.0	5.443073082790182	0.0
34	0.0015408557913064917	0.0	0.0	5.6372209124948	0.0
35	0.0015408557913064917	0.0	0.0	5.815960184286353	0.0
36	0.0015408557913064917	0.0	0.0	6.01704186505185	0.0
37	0.0015408557913064917	0.0	0.0	6.194240281052096	0.0
38	0.0015408557913064917	0.0	0.0	6.386076827069754	0.0
39	0.0015408557913064917	0.0	0.0	6.584847224148292	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	20	7.0101465E-4	45.000004	5
CGGGTGT	20	7.0101465E-4	45.000004	6
CAGTTGG	20	7.0101465E-4	45.000004	2
GGACTGC	20	7.0101465E-4	45.000004	8
GGACTCA	20	7.0101465E-4	45.000004	8
TACGGGA	80	0.0	45.000004	4
TAACGGG	30	2.1505912E-6	45.000004	3
ACGGGCA	20	7.0101465E-4	45.000004	5
TATTGCG	20	7.0101465E-4	45.000004	1
TACGAAT	40	6.7357178E-9	45.000004	12
CGGGACC	40	6.7357178E-9	45.000004	6
CGGGACA	30	2.1505912E-6	45.000004	6
CGTAAGG	20	7.0101465E-4	45.000004	2
ATACGGG	40	6.7357178E-9	45.000004	3
GTGACGG	20	7.0101465E-4	45.000004	2
AGGGCCG	20	7.0101465E-4	45.000004	6
TGAGCGG	30	2.1505912E-6	45.000004	2
ATGCATA	30	2.1505912E-6	45.000004	26
AGTACGG	20	7.0101465E-4	45.000004	2
CCAGGGA	25	3.871639E-5	45.0	4
>>END_MODULE