FastQCFastQC Report
Sat 18 Jun 2016
SRR3549364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85000
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8721.0258823529411765No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.24588235294117647No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1700.2No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1510.1776470588235294No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT1260.14823529411764708No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1250.14705882352941177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC351.1942575E-745.0000045
CTTGGTC351.1942575E-745.00000429
CGGGATC351.1942575E-745.0000046
GACGGGA750.045.0000044
AGGGACC700.045.0000046
ATGGGCT453.765308E-1045.05
CTATGGG453.765308E-1045.03
ACACGGG206.99644E-445.03
CGCGGGA206.99644E-445.04
CGACGGG253.860296E-545.03
ATGGCCT253.860296E-545.011
TGGACGG206.99644E-445.02
TATGGCC253.860296E-545.010
GACCGAT206.99644E-445.09
ATAGCGG206.99644E-445.02
GGATCTA253.860296E-545.08
GCACGGG206.99644E-445.03
AATGGGC206.99644E-445.04
AGGGTGC206.99644E-445.06
TAACGGG206.99644E-445.03